Substituted aminothiazoles as dgkzeta inhibitors for immune activation

ABSTRACT

The present invention covers aminothiazole compounds of general formula (I), in which R1, R2, R3 and R4 are as defined herein, methods of preparing said compounds, intermediate compounds useful for preparing said compounds, pharmaceutical compositions and combinations comprising said compounds and the use of said compounds for manufacturing pharmaceutical compositions for the treatment and/or prophylaxis of diseases, in particular of diacylglycerol kinase zeta (DGKζ) regulated disorders, as a sole agent or in combination with other active ingredients.

The present invention covers substituted aminothiazole compounds of general formula (I) as described and defined herein, methods of preparing said compounds, intermediate compounds useful for preparing said compounds, pharmaceutical compositions and combinations comprising said compounds, and the use of said compounds for manufacturing pharmaceutical compositions for the treatment or prophylaxis of diseases, in particular of diacylglycerol kinase zeta (DGKzeta, DGKζ) regulated disorders, as a sole agent or in combination with other active ingredients.

The compounds of general formula (I) inhibit DGKζ and, by this, enhance T cell mediated immune response. This is a new strategy to use the patient's own immune system to overcome immunoevasive strategies utilized by many neoplastic disorders, respectively cancer and by this enhancing anti-tumor immunity. Furthermore, said compounds are used in particular to treat disorders such as viral infections or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling.

The present invention further relates to the use of the compounds of general formula (I) for manufacturing pharmaceutical compositions for enhancement of T cell mediated immune response.

The present invention further relates to the use of the compounds of general formula (I) for manufacturing pharmaceutical compositions for the treatment of cancer.

The present invention further relates to the use of the compounds of general formula (I) for manufacturing pharmaceutical compositions for the treatment or prophylaxis of virus infections, lymphoproliferative disorders, asthma, eye diseases, and type 2 diabetes/insulin resistance.

BACKGROUND

Diacylglycerol kinases (DGKs) represent a family of enzymes that catalyze phosphorylation of the membrane lipid sn-1,2 diacylglycerol (DAG) to form phosphatidic acid (PA) (T. O. Eichmann and A. Lass, Cell. Mol. Life Sci. 2015, 72, 3931-3952). In T cells, DAG is formed downstream of the T cell receptor (TCR) after activation of the gamma 1 isoform of phospholipase C (PLCγ1) and cleavage of phosphatidylinositol 4,5-biphosphate (PIP2) into DAG and an additional second messenger, inositol 1,4,5-triphosphate (IP3) (S. Krishna and X. P. Zhong, Front. Immunol. 2013, 4, 178). Whereas, IP3 is important in facilitating release of calcium from the endoplasmic reticulum, DAG interacts with other proteins important in TCR signal transduction, such as Protein kinase Cθ (E. J. Quann et al., Nat. Immunol. 2011, 12 (7), 647-654) and the Ras activating protein RasGRP1 (S. Krishna and X. P. Zhong, Front. Immunol. 2013, 4, 178). Although, three isoforms of DGKζ are known to be present within T cells [DGKα (DGKalpha), DGKδ (DGKdelta), and DGKζ (DGKzeta)], only two, DGKα and DGKζ, are thought to play an important role in facilitating DAG metabolism downstream of the TCR (R. P. Joshi and G. A. Koretzky, Int. J. Mol. Sci. 2013, 14 (4), 6649-6673).

Targeting the activity of DGKζ in T cells, either by germline deletion, or with chemical inhibitors, results in enhanced and sustained signaling downstream of T cells, as assessed by prolonged phosphorylation of downstream molecules, such as extracellular signal-related kinases 1/2 (ERK1/2) and NFκB (X. P. Zhong et al., Nat. Immunol. 2003, 4, 882-890; B. A. Olenchock et al., Nat. Immunol. 2006, 7 (11), 1174-1181; M. J. Riese et al., J. Biol. Chem. 2011, 286, 5254-5265; E. M. Wesley et al., ImmunoHorizons 2018, 2 (4), 107-118).

Deletion of DGKζ in T cells leads to enhanced production of effector cytokines, such as IL2, IFNγ and enhanced proliferation (X. P. Zhong et al., Nat. Immunol. 2003, 4, 882-890; B. A. Olenchock et al., Nat. Immunol. 2006, 7 (11), 1174-1181, E. M. Riese et al., J. Biol. Chem. 2011, 286, 5254-5264).

Adoptive transfer of DGK; deficient T cell reduced leukaemia burden after inoculation of C1498. SIY leukaemia cells compared to control. Also, DGKζ deficient T cells are at least partially resistant to PD1 mediated inhibitory signals (W. Jing et al., Cancer Res. 2017, 77 (20), 5676-5686). In addition, DGKζ deficient mice have reduced tumor sizes compared to control after orthotopic tumor injection of a pancreatic tumor model (E. M. Wesley et al., ImmunoHorizons, 2018, 2 (4), 107-118). Also, S. Wee et al. inoculated C57BL/6 mice with a variety of syngeneic tumor cell lines—MC38 colon carcinoma, B16F1 melanoma, and C1498 leukemia—and analysed survival and tumor growth between mice deficient in DGKζ in the presence or absence of anti-PD1 treatment. DGKζ−/− mice suppressed growth of subcutaneously implanted tumor cells in the three model systems and the combination of DGKζ-deficiency and anti-PD1 was additive in tumor control (S. Wee et al., Proceedings of the American Association for Cancer Research Annual Meeting 2019; Cancer Res. 2019, 79 (13 Suppl): Abstract nr 936).

These findings suggest that DGKζ might serve as a useful target for enhancing T cell anti-tumor activity.

Additionally, the adoptive transfer of CAR (chimeric antigen receptor)-T cells deficient in DGKζ demonstrated increased efficacy compared to wild type CAR T cells in the treatment of murine mesothelioma (M. J. Riese et al., Cancer Res. 2013, 73 (12), 3566-3577) and a glioblastoma xenograft mouse model in combination with DGKα knockout (I. Y. Jung et al., Cancer Res. 2018, 78 (16), 4692-4703).

Also, DGKζ-deficient mice mounted a more robust immune response to lymphocytic choriomeningitis virus infection than did wild-type mice (X. P. Zhong et al., Nat. Immunol. 2003, 4, 882-890).

DGKζ is also relevant in natural killer (NK) cells. Upon stimulation through multiple activating receptors, NK cells from mice lacking DGKζ display increased cytokine production and degranulation in an ERK-dependent manner. Additionally, they have improved cytotoxic functions against tumor cell lines. (E. Yang et al. J. Immunol. 2016, 197(3), 934-41.) Apart from immune-cell regulation, DGKα also plays a role in cancer, mediating numerous aspects of cancer cell progression including proliferation, apoptosis, survival, invasion and tumorigenicity, e.g. in osteosarcoma, colon cancer, breast cancer, prostate cancer, glioma and leukemia models (W. Yu et al., Front. Oncol. 2019, 8:655; K. Cai et al., BMC Cancer 2014, 14:208; J. Diao et al., Mol. Neurobiol. 2016; 53, 5425-35; H. Li et al. Pharmazie 2019, 74(7): 418-422).

In addition, DGKζ knock-out diminished both airway inflammation and airway hyperresponsiveness in mice and also reduced bronchoconstriction of human airway samples in vitro by blocking T helper 2 (TH2) differentiation (B. A. Singh et al., Sci. Signal. 2019, 12: eaax3332).

Taken together, the findings from these studies argue that restraining DGKζ activity in T cells and tumor cells may prove valuable in generating more vigorous immune responses against pathogens and tumors and in ameliorating Th2 driven (auto-) immune diseases (in re-balancing the immune-system).

Furthermore, inhibition of DGKα has the potential to reverse the life-threatening Epstein-Barr virus (EBV)-associated immunopathology that occurs in X-linked lymphoproliferative disease (XLP-1) patients (E. Ruffo et al., Sci. Transl. Med. 2016, 8: (321):321ra7; S. Velnati et al., Eur. J. Med. Chem. 2019, 164, 378-390). Based on the underlying mode of action, it can be assumed that inhibition of DGKζ would have a similar effect.

The DGKα-inhibitor II (R59949) could suppress retinal neovascularization and protect retinal astrocytes in an Oxygen-Induced Retinopathy Model (L. Yang et al., J. Mol. Neurosci. 2015, 56, 78-88). Also, based on the underlying mode of action, it can be assumed that inhibition of DGK; would have a similar effect.

In a DGKζ knock-out mouse it was shown that DGKζ deficiency increases protection against insulin resistance (B. Benziane et al., J. Lipid Res. 2017, 58 (12), 2324-2333).

In summary, inhibiting DGKζ activity has a therapeutic potential in targeting tumors directly as well as addressing virus infections, lymphoproliferative disorders, asthma, eye diseases, and type 2 diabetes/insulin resistance.

PRIOR ART

WO2020/006016 and WO2020/006018 describe Naphthyridinone compounds as T cell activators, which inhibit the activity of DGKα and/or DGKζ, for treatment of viral infections and proliferative disorders, such as cancer. WO2021/041588 describes pyridopyrimidinyl compounds as T cell activators, which inhibit the activity of DGKα and/or DGKζ, for treatment of viral infections and proliferative disorders, such as cancer.

-   2,4,5-trisubstituted triazole derivatives featuring a substituted     amino group attached to C-2 of the thiazole core have been disclosed     in published patent applications in various technical contexts but     not in the context of DGKζ inhibition.

WO 2014/181287 discloses heterocyclyl compounds as inhibitors of Interleukin 17 and Tumour Necrosis Factor alpha.

WO 2014/173904 discloses compounds having antibacterial activity.

WO 2009/149054 discloses small molecule inhibitors for the treatment or prevention of Dengue fever infection.

WO 2007/130075 discloses aminothiazole derivatives as human stearoyl-CoA desaturase inhibitors.

WO 2012/064715 discloses compositions and methods relating to heat shock transcription factor activating compounds and targets thereof.

WO 2005/103022 discloses substituted thiazole and pyrimidine derivatives as melanocortin receptor modulators.

CN 106109467 discloses the medical application of aromatic compounds, including thiazoles, in treating pyrazinamide-resistant tuberculosis.

WO 2015/199206 discloses compounds derived from a six-membered ring as TRPV4 inhibitors.

WO 2015/046193 discloses aromatic heterocyclic amine derivatives as TRPV4 inhibitors.

CN 103159695 discloses thiazole compounds capable of restraining human immunodeficiency (HIV) virus replication and effective against drug-resistant HIV virus strains.

WO 2013/056684 discloses thiazole derivatives as dihydroorotate dehydrogenase (DHODH) inhibitors.

WO 2013/033037 discloses compounds of various chemotypes, inter alia thiazole derivatives, as antiprion compounds.

WO 2012/075393 discloses compounds of various chemotypes, inter alia thiazole derivatives, as activators of proteasomal degradation.

JP 2011032254 discloses compounds of various chemotypes, inter alia thiazole derivatives, as pest controlling agents.

WO 2009/041790 discloses 2,4,5-trisubstituted thiazole derivatives as inhibitors of the sphingosylphosphorylcholine (SPC) receptor for treatment of inflammatory diseases.

WO 2008/090382 discloses thiazole and oxazole derivatives for use in the treatment of prion diseases, cancer, and conditions of the central nervous system as well as in the regulation of stem cells.

WO 2007/022415 discloses substituted 2-aminothiazoles for treating neurodegenerative diseases.

WO 2006/122011 discloses thiazole compounds and methods of use in treating viral infections, particularly hepatitis C virus infections.

WO 2006/078287 discloses derivatives of various 5-membered heteroarenes, inter alia thiazoles, as inhibitors of phosphodiesterase 4B.

WO 2005/102318, WO 2005/102325, WO 2005/102326, WO 2005/102346, WO 2005/102455, WO 2005/112920, WO 2005/115304, WO 2005/115385 all deal with c-Kit inhibitors of various chemotypes, including 2-aminothiazole derivatives, and various uses thereof.

EP 1543824 and US 2005/0137239 disclose thiazole derivatives to counter glycation.

WO 2004/014884 discloses thiazole derivatives as neuropeptide Y receptor ligands.

WO 2019/133445 discloses aminothiazole derivatives as inhibitors of Vanin-1.

WO 2021/043966 discloses substituted five-membered nitrogen containing heteroaryl compounds as inhibitors of the NOD-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3).

2,4,5-trisubstituted triazole derivatives structurally related to the compounds of the present invention but yet structurally distinct have also been disclosed in several scientific publications.

D. Kikelj and U. Urleb, Science of Synthesis (2002), 11, 627-833, is a general review on the synthesis of thiazoles. By far most of the specific compounds disclosed are, besides having the thiazole core in common with the compounds of the present invention, structurally distant from these. Several individual compounds disclosed therein, namely those disclosed on pp 651, 681-683, and 719, and including {4-methyl-2-[methyl(phenyl)amino]-1,3-thiazol-5-yl}(phenyl)methanone, are somewhat structurally related to the compounds of the present invention but yet structurally distinct.

2,4,5-trisubstituted triazole derivatives structurally related to the compounds of the present invention but yet structurally distinct have also been disclosed in several journal articles.

None of the journal articles listed below, which also disclose some compounds which are somewhat structurally related to the compounds of the present invention but yet structurally distinct, disclose a therapeutic of pharmaceutical application of the compounds disclosed therein.

S. Titus et al., Tetrahedron Lett. 2014, 55, 5465-5467, disclose a four-component synthesis of 4-hydrazinothiazole derivatives.

T. N. Birkinshaw et al., J. Chem. Soc. Perkin Trans. 1, 1988, 2209-2212, disclose spectrometric and chemical studies on 5-acyl and 5-nitroso-2-(N,N-disubstituted amino)thiazoles.

R. A. Funnell et al., J. Chem. Soc. Perkin Trans. 1, 1987, 2311-2315 disclose a study on the formation of isomeric 2-(N,N-disubstituted amino) thiazol-5-yl ketones.

J. C. Brindley et al., J. Chem. Soc. Perkin Trans. 1, 1987, 1153-1158 disclose the conversion of N′-substituted N-acyl and N-imidoyl thioureas into 2-(N,N-disubstituted amino) thiazol-5-yl ketones.

G. D. Meakins et al., J. Chem. Soc. Chem. Comm. 1984, 837-838, disclose a chemical reaction unexpectedly yielding 5-benzoyl-4-methyl-2-(N-methyl-N-phenylamino)thiazole.

K. Akiba et al., Tetrahedron Lett. 1975, 7, 459-462, disclose the synthesis of certain 2-arylaminothiazoles by 1,3-cycloaddition of 4-aryl-3-arylimino-5-imino-1,2-4-thiadiazolidine (also known as Hector's base) and acetylenes.

Finally, three structures which are somewhat structurally related to the compounds of the present invention but yet structurally distinct, are disclosed in the database Chemical Abstracts Registry (CAS Registry®), all without a reference and without a technical application, having the CAS Registry numbers 1349635-19-3, 1349215-57-1, and 1348293-02-6.

However, the state of the art does not describe:

-   -   the specific substituted aminothiazole compounds of general         formula (I) of the present invention as described and defined         herein, i.e. compounds having a 2-aminothiazole core bearing:         -   an optionally substituted benzoyl or 6-membered heteroaroyl             group attached to C-5,         -   a —NH₂ or methyl group attached to C₁₋₄,         -   and (i) an optionally substituted phenyl or 5- or 6-membered             heteroaryl group and (ii) an alkyl group laterally             substituted with a group —S(═O)₂—NH₂, or, particularly,             —C(═O)—NH₂, both (i) and (ii) being attached to the nitrogen             atom attached to C-2, said laterally substituted alkyl group             being essential for potent inhibition of DGKζ as shown in             comparative experiments, infra, or stereoisomers, tautomers,             N-oxides, hydrates, solvates, salts thereof, or mixtures of             same, as described and defined herein, and as hereinafter             referred to as “compounds of general formula (I)” or             “compounds of the present invention”,     -   or their pharmacological activity.

WO 2021/028382, relating to a different chemotype ([1,2,4]triazolo[1.5-c]quinazolin-5-amine compounds) according to formula (I) and abstract, discloses a single compound name N²-(4-amino-5-benzoyl-1,3-thiazol-2-yl)-N²-(3-methylphenyl)alaninamide, without providing a synthesis protocol and without biological data relating to said compound name.

It is desirable to provide novel compounds having prophylactic and therapeutic properties.

Accordingly, it is an object of the present invention to provide compounds and pharmaceutical compositions comprising these compounds used for prophylactic and therapeutic use in DGKζ regulated disorders in a T cell immune-stimulatory or immune-modifying manner. DGKζ regulated disorders comprise conditions with dysregulated immune responses, particularly in an immunologically suppressed tumor microenvironment in cancer, autoimmune diseases, viral infections as well as other disorders associated with aberrant DGKζ signalling. Said compounds can be used as sole agent or in combination with other active ingredients.

It has now been found, and this constitutes the basis of the present invention, that the compounds of the present invention have surprising and advantageous properties.

In particular, the compounds of the present invention have surprisingly been found to effectively inhibit the DGKζ protein and, by this, enhance T-cell mediated immunity. Accordingly, they provide novel structures for the treatment diseases of mammals, including humans, in particular of cancers, and may therefore be used for the treatment or prophylaxis of hyperproliferative disorders, such as cancer, for example.

DESCRIPTION OF THE INVENTION

In accordance with a first aspect, the present invention covers compounds of general formula (I):

in which:

-   -   R¹ represents a phenyl or 6-membered heteroaryl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from hydroxy, cyano, nitro, C₁-C₆-alkyl,         (phenyl)-(C₁-C₃-alkyl)-, C₁-C₆-haloalkyl, C₁-C₆-alkoxy,         (phenyl)-(C₁-C₃-alkoxy)-, C₁-C₆-haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₃-alkyl)-             and (phenyl)-(C₁-C₃-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a halogen atom or a group selected from cyano,             methyl, ethyl, trifluoromethyl, methoxy, ethoxy,             dimethylamino and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or 6-membered heteroaryl group together form a             bivalent group selected from —(CH₂)₃—, —(CH₂)₄—, (CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —(CH₂)₂O—CH₂—, —O—CH₂—O—,             —O—CH₂—CH₂—O—, —O—CF₂—O—, —O—CH₂—CF₂—O—, and —O—CF₂—CF₂—O—,             or     -   R¹ represents a 5-membered heteroaryl group optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from cyano,         C₁-C₃-alkyl, and C₁-C₃-alkoxy;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or 6-membered heteroaryl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₆-alkyl, (phenyl)-(C₁-C₃-alkyl)-, (5- or         6-membered heteroaryl)-(C₁-C₃-alkyl)-,         (C₃-C₇-cycloalkyl)-(C₁-C₃-alkyl)-, ((R⁹)O)—(C₁-C₆-alkyl)-,         C₁-C₆-haloalkyl, C₃-C₇-cycloalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         ((R¹⁰)(R¹¹)N)—(C₁-C₃-alkyl)-, —C(═O)—N(R¹²)(R¹³), —S(═O)˜-R¹⁴,         —C(═O)R¹⁴, —C(═O)—OR⁷, and a 5- or 6-membered heteroaryl group         which itself is optionally substituted with one or two         substituents selected from a halogen atom and a methyl group,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or 6-membered heteroaryl group together form a             bivalent group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —(CH₂)₂—O—CH₂—, —O—CH₂—O—,             —O—CH₂—CH₂—O—, —O—CF₂—O—, —O—CH₂—CF₂—O—, and —O—CF₂—CF₂—O—;     -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl and         (phenyl)-(C₁-C₃-alkyl)-, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from oxo, hydroxy, C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl and C₁-C₄-alkoxy;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a group selected from —C(═O)—NH₂ and —S(═O)₂—NH₂;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, (5- or 6-membered heteroaryl)-(C₁-C₃-alkyl)-,         (phenyl)-(C₁-C₃-alkyl)-, C₁-C₆-haloalkyl, C₂-C₄-hydroxyalkyl,         (C₁-C₃-alkoxy)-C₂-C₃-alkyl-,         ((C₁-C₃-alkyl)-C(═O)—O)—C₂-C₃-alkyl-, —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹), —C(═O)—N(R²⁰)(R²¹), phenyl and         5- or 6-membered heteroaryl group,         -   wherein the phenyl group within said             (phenyl)-(C₁-C₃-alkyl)-group and said phenyl group itself,             and the 5- or 6-membered heteroaryl group within said (5- or             6-membered heteroaryl)-(C₁-C₃-alkyl)-group and said 5- or             6-membered heteroaryl group itself are optionally             substituted one or two times, each substituent independently             selected from a halogen atom or a group selected from cyano,             methyl, ethyl, trifluoromethyl, methoxy, ethoxy,             dimethylamino and trifluoromethoxy;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₂-C₄-hydroxyalkyl,         (C₁-C₃-alkoxy)-C₂-C₃-alkyl-, ((R²²)(R²³)N)—C₂-C₃-alkyl,         (C₃-C₇-cycloalkyl)-(C₁-C₃-alkyl)-, (C₁-C₄-alkyl)-C(═O)—,         C₃-C₇-cycloalkyl, (C₃-C₇-cycloalkyl)-C(═O)—,         (phenyl)-(C₁-C₃-alkyl)-, (phenyl)-(C₁-C₃-alkyl)-C(═O)—,         (phenyl)-(C₁-C₃-alkyl)-O—C(═O)—, phenyl and a 5- or 6-membered         heteroaryl group,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₇-cycloalkyl within             said (C₃-C₇-cycloalkyl)-(C₁-C₃-alkyl)- and             (C₃-C₇-cycloalkyl)-C(═O)— groups are optionally substituted             one or two times, each substituent independently selected             from a fluorine atom or a group selected from cyano,             C₁-C₂-alkyl and C₁-C₂-haloalkyl,         -   and wherein said phenyl and said 5- or 6-membered heteroaryl             group, and the phenyl groups within said             (phenyl)-(C₁-C₃-alkyl)-, (phenyl)-(C₁-C₃-alkyl)-C(═O)— and             (phenyl)-(C₁-C₃-alkyl)-O—C(═O)— groups, are optionally             substituted one or two times, each substituent independently             selected from a halogen atom or a group selected from cyano,             methyl, ethyl, trifluoromethyl, methoxy, ethoxy,             dimethylamino and trifluoromethoxy, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 5- to 11-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₄-alkyl, C₁-C₄-haloalkyl,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₇-cycloalkyl, C₁-C₄-alkoxy,         —N(R²²)(R²³), and a monocyclic 4- to 7-membered heterocycloalkyl         group;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₄-haloalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, oxo, hydroxy,             C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl and             C₁-C₄-alkoxy,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₃-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             halogen atom or a group selected from cyano, methyl, ethyl,             trifluoromethyl, methoxy, ethoxy, dimethylamino and             trifluoromethoxy, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl and C₁-C₄-alkoxy;     -   R¹⁴ represents a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl and phenyl, wherein the phenyl group is         optionally substituted one or two times, each substituent         independently selected from a halogen atom or a group selected         from cyano, methyl, ethyl, trifluoromethyl, methoxy, ethoxy,         dimethylamino and trifluoromethoxy;     -   R¹⁷ represents a C₁-C₄-alkyl group; R¹⁸ and R¹⁹ represent,         independently from each occurrence, a hydrogen atom or a         C₁-C₄-alkyl group;     -   R²⁰ represents a hydrogen atom or a group selected from C₁-C₆         alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₅-C₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 5- to         11-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³), C₃-C₇cycloalkyl,             bicyclic C₅-C₁₁-cycloalkyl, adamantyl, monocyclic 4- to             7-membered heterocycloalkyl, bicyclic 5- to 11-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a halogen atom             or a group selected from cyano, methyl, ethyl,             trifluoromethyl, methoxy, ethoxy, dimethylamino and             trifluoromethoxy, and wherein C₃-C₇-cycloalkyl, bicyclic             C₅-C₁₁-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl and bicyclic 5- to 11-membered             heterocycloalkyl are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, oxo, hydroxy,             C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl and             C₁-C₄-alkoxy,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₄-alkyl,             C₁-C₄-haloalkyl, C₁-C₄-alkoxy, C₁-C₄-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵)     -   R²¹ represents a hydrogen atom or a C₁-C₄-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₄-alkyl, C₁-C₄-haloalkyl, (phenyl)-(C₁-C₃-alkyl)-,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₄-alkoxy,         C₁-C₃-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₄alkyl group, and     -   n represents an integer 0, 1, or 2, and stereoisomers,         tautomers, N-oxides, hydrates, solvates, and salts thereof, and         mixtures of same.

Definitions

The term “substituted” means that one or more hydrogen atoms on the designated atom or group are replaced with a selection from the indicated group, provided that the designated atom's normal valency under the existing circumstances is not exceeded. Combinations of substituents and/or variables are permissible.

The term “optionally substituted” means that the number of substituents can be equal to or different from zero. Unless otherwise indicated, it is possible that optionally substituted groups are substituted with as many optional substituents as can be accommodated by replacing a hydrogen atom with a non-hydrogen substituent on any available carbon or nitrogen atom. Commonly, it is possible for the number of optional substituents, when present, to be 1, 2, 3 or 4, in particular 1, 2 or 3.

When groups in the compounds according to the invention are substituted, it is possible for said groups to be mono-substituted or poly-substituted with substituent(s), unless otherwise specified. Within the scope of the present invention, the meanings of all groups which occur repeatedly are independent from one another. It is possible that groups in the compounds according to the invention are substituted with one, two or three identical or different substituents, particularly with one substituent.

As used herein, an oxo substituent represents an oxygen atom, which is bound to a carbon atom or to a sulfur atom via a double bond.

Should a composite substituent be composed of more than one part, e.g. (C₁-C₂-alkoxy)-(C₁-C₆-alkyl)-, it is possible for a given part to be attached at any suitable position of said composite substituent, e.g. it is possible for the C₁-C₂-alkoxy part to be attached to any suitable carbon atom of the C₁-C₅-alkyl part of said (C₁-C₂-alkoxy)-(C₁-C₆-alkyl)-group. A hyphen at the beginning or at the end of such a composite substituent indicates the point of attachment of said composite substituent to the rest of the molecule. Should a ring, comprising carbon atoms and optionally one or more heteroatoms, such as nitrogen, oxygen or sulfur atoms for example, be substituted with a substituent, it is possible for said substituent to be bound at any suitable position of said ring, be it bound to a suitable carbon atom and/or to a suitable heteroatom.

The term “comprising” when used in the specification includes “consisting of”.

If within the present text any item is referred to as “as mentioned herein”, it means that it may be mentioned anywhere in the present text.

The terms as mentioned in the present text have the following meanings:

The term “halogen atom” means a fluorine, chlorine, bromine or iodine atom, particularly a fluorine, chlorine or bromine atom.

The term “C₁-C₆-alkyl” means a linear or branched, saturated, monovalent hydrocarbon group having 1, 2, 3, 4, 5 or 6 carbon atoms, e.g. a methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, isobutyl, tert-butyl, pentyl, isopentyl, 2-methylbutyl, 1-methylbutyl, 1-ethylpropyl, 1,2-dimethylpropyl, neo-pentyl, 1,1-dimethylpropyl, hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1-ethylbutyl, 2-ethylbutyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2,3-dimethylbutyl, 1,2-dimethylbutyl or 1,3-dimethylbutyl group, or an isomer thereof. Particularly, said group has 1, 2, 3 or 4 carbon atoms (“C₁-C₄-alkyl”), e.g. a methyl, ethyl, propyl, isopropyl, butyl, sec-butyl isobutyl, or tert-butyl group, more particularly 1, 2 or 3 carbon atoms (“C₁-C₃-alkyl”), e.g. a methyl, ethyl, n-propyl or isopropyl group, more particularly 1 or 2 carbon atoms (“C₁-C₂-alkyl”), e.g. a methyl or ethyl group.

The term “C₁-C₄-hydroxyalkyl” means a linear or branched, saturated, monovalent hydrocarbon group in which the term “C₁-C₄-alkyl” is defined supra, and in which 1 or 2 hydrogen atoms are replaced with a hydroxy group, e.g. a hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl, 1,2-dihydroxyethyl, 3-hydroxypropyl, 2-hydroxypropyl, 1-hydroxypropyl, 1-hydroxypropan-2-yl, 2-hydroxypropan-2-yl, 2,3-dihydroxypropyl, 1,3-dihydroxypropan-2-yl, 3-hydroxy-2-methyl-propyl, 2-hydroxy-2-methyl-propyl, 1-hydroxy-2-methyl-propyl, 1-hydroxybutyl, 2-hydroxybutyl, 3-hydroxybutyl, 4-hydroxybutyl group, or an isomer thereof.

The term “C₁-C₆-haloalkyl” means a linear or branched, saturated, monovalent hydrocarbon group in which the term “C₁-C₆-alkyl” is as defined supra, and in which one or more of the hydrogen atoms are replaced, identically or differently, with a halogen atom. Particularly, said halogen atom is a fluorine atom. Said C₁-C₆-haloalkyl group is, for example, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, pentafluoroethyl, 3,3,3-trifluoropropyl or 1,3-difluoropropan-2-yl.

The term “C₁-C₆-alkoxy” means a linear or branched, saturated, monovalent group of formula (C₁-C₆-alkyl)-O—, in which the term “C₁-C₆-alkyl” is as defined supra, e.g. a methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, sec-butoxy, isobutoxy, tert-butoxy, pentyloxy, isopentyloxy or n-hexyloxy group, or an isomer thereof.

The term “C₁-C₆-haloalkoxy” means a linear or branched, saturated, monovalent C₁-C₆-alkoxy group, as defined supra, in which one or more of the hydrogen atoms is replaced, identically or differently, with a halogen atom. Particularly, said halogen atom is a fluorine atom. Said C₁-C₆-haloalkoxy group is, for example, fluoromethoxy, difluoromethoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy or pentafluoroethoxy.

The term “C₃-C₄-alkenyl” means a linear or branched, monovalent hydrocarbon group, which contains one or two double bonds, and which has 3 or 4 carbon atoms. Said alkenyl group is, for example, a prop-2-en-1-yl (or “allyl”), prop-1-en-1-yl, but-3-enyl, but-2-enyl or but-1-enyl group.

The term “C₃-C₄-alkynyl” means a linear or branched, monovalent hydrocarbon group which contains one triple bond, and which contains 3 or 4 carbon atoms. Said C₁-C₄-alkynyl group is, for example, a prop-1-ynyl, prop-2-ynyl (or “propargyl”), but-1-ynyl, but-2-ynyl or but-3-ynyl group.

The term “C₃-C₇-cycloalkyl” means a saturated, monovalent, monocyclic hydrocarbon ring which contains 3, 4, 5, 6 or 7 carbon ring atoms (“C₃-C₇-cycloalkyl”). Said C₃-C₇-cycloalkyl group is for example, a cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl group.

The term “bicyclic C₆-C₁₁-cycloalkyl” means a spirocycloalkyl, fused C₆-C₁₀-cycloalkyl or bridged C₇-C₁₀-cycloalkyl group as defined below:

-   -   The term “spirocycloalkyl” means a bicyclic, saturated,         monovalent C₅-C₁₁ hydrocarbon group in which the two rings share         one common ring carbon atom, and wherein said bicyclic         hydrocarbon group contains 5, 6, 7, 8, 9, 10 or 11 carbon atoms,         it being possible for said spirocycloalkyl group to be attached         to the rest of the molecule via any one of the carbon atoms         except the spiro carbon atom. Said spirocycloalkyl group is, for         spiro[2.6]nonyl, spiro[3.3]heptyl, spiro[3.4]octyl,         spiro[3.5]nonyl, spiro[3.6]decyl, spiro[4.4]nonyl,         spiro[4.5]decyl, spiro[4.6]undecyl or spiro[5.5]undecyl.     -   The term “fused C₆-C₁₀-cycloalkyl” means a bicyclic, saturated,         monovalent hydrocarbon group, in which the two rings share two         adjacent ring atoms, such as bicyclo[4.2.0]octyl,         octahydropentalenyl or decalinyl.     -   The term “bridged C₇-C₁₀-cycloalkyl” means a bicyclic,         saturated, monovalent hydrocarbon group which the two rings         share two common ring atoms which are not adjacent, e.g.         bicyclo[2.2.1]heptyl (also known as norbornyl).

The term “bicyclic C₅-C₁₁-cycloalkyl” means a spirocycloalkyl, fused C₅-C₁₀-cycloalkyl or bridged C₅-C₁₀-cycloalkyl group as defined below:

-   -   The term “spirocycloalkyl” means a bicyclic, saturated,         monovalent C₅-C₁₁ hydrocarbon group in which the two rings share         one common ring carbon atom, and wherein said bicyclic         hydrocarbon group contains 5, 6, 7, 8, 9, 10 or 11 carbon atoms,         it being possible for said spirocycloalkyl group to be attached         to the rest of the molecule via any one of the carbon atoms         except the spiro carbon atom. Said spirocycloalkyl group is, for         spiro[2.6]nonyl, spiro[3.3]heptyl, spiro[3.4]octyl,         spiro[3.5]nonyl, spiro[3.6]decyl, spiro[4.4]nonyl,         spiro[4.5]decyl, spiro[4.6]undecyl or spiro[5.5]undecyl.     -   The term “fused C₅-C₁₀-cycloalkyl” means a bicyclic, saturated,         monovalent hydrocarbon group, in which the two rings share two         adjacent ring atoms, such as bicyclo[4.2.0]octyl,         octahydropentalenyl or decalinyl.     -   The term “bridged C₅-C₁₀-cycloalkyl” means a bicyclic,         saturated, monovalent hydrocarbon group which the two rings         share two common ring atoms which are not adjacent, e.g.         bicyclo[1.1.1]pentyl or bicyclo[2.2.1]heptyl (also known as         norbornyl).

The term “monocyclic 4- to 7-membered heterocycloalkyl” means a monocyclic, saturated heterocycle with 4, 5, 6 or 7 ring atoms in total, which contains one or two identical or different ring heteroatoms from the series N, O and S.

Said monocyclic heterocycloalkyl group, without being limited thereto, can be a 4-membered ring, such as azetidinyl, oxetanyl or thietanyl, for example; or a 5-membered ring, such as tetrahydrofuranyl, 1,3-dioxolanyl, thiolanyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, 1,1-dioxidothiolanyl, 1,2-oxazolidinyl, 1,3-oxazolidinyl or 1,3-thiazolidinyl, for example; or a 6-membered ring, such as tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, morpholinyl, dithianyl, thiomorpholinyl, piperazinyl, 1,3-dioxanyl, 1,4-dioxanyl or 1,2-oxazinanyl, for example, or a 7-membered ring, such as azepanyl, 1,4-diazepanyl or 1,4-oxazepanyl, for example.

The term “monocyclic nitrogen containing 4- to 7-membered heterocycloalkyl group” means a monocyclic, saturated heterocycle with 4, 5, 6 or 7 ring atoms in total, which contains one ring nitrogen atom and optionally one further ring heteroatom from the series N, O and S.

Said monocyclic nitrogen containing 4- to 7-membered heterocycloalkyl group, without being limited thereto, can be a 4-membered ring, such as azetidinyl, for example; or a 5-membered ring, such as pyrrolidinyl, imidazolidinyl, pyrazolidinyl, 1,2-oxazolidinyl, 1,3-oxazolidinyl or 1,3-thiazolidinyl, for example; or a 6-membered ring, such as piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or 1,2-oxazinanyl, for example, or a 7-membered ring, such as azepanyl, 1,4-diazepanyl or 1,4-oxazepanyl, for example.

The term “monocyclic nitrogen containing 4- to 7-membered heterocycloalkyl group which is optionally benzocondensed” means a monocyclic, saturated heterocycle with 4, 5, 6 or 7 ring atoms in total, which contains one ring nitrogen atom and optionally one further ring heteroatom from the series N, O and S, in which two adjacent ring carbon atoms may be shared with a benzene ring optionally fused thereto, such group being one of the aforementioned monocyclic nitrogen containing 4- to 7-membered heterocycloalkyl groups, such as pyrrolidinyl, piperidinyl, and the like, or benzocondensed groups e.g. 3,4-dihydroquinolin-1 (2H)-yl, 3,4-dihydroisoquinolin-2 (1H)-yl, 1,3-dihydro-2H-isoindol-2-yl or 2,3-dihydro-1H-indol-1-yl.

The term “bicyclic 6-11 membered heterocycloalkyl” means a 6- to 11-membered heterospirocycloalkyl, a 6- to 10-membered fused heterocycloalkyl or a 7- to 10-membered bridged heterocycloalkyl group as defined below:

-   -   The term “6- to 11-membered heterospirocycloalkyl” means a         bicyclic, saturated heterocycle with 6, 7, 8, 9, 10 or 11 ring         atoms in total, in which the two rings share one common ring         carbon atom, which “heterospirocycloalkyl” contains one or two         identical or different ring heteroatoms from the series: N, O,         S; it being possible for said heterospirocycloalkyl group to be         attached to the rest of the molecule via any one of the carbon         atoms, except the spiro carbon atom, or, if present, a nitrogen         atom.     -   Said heterospirocycloalkyl group is, for example,         azaspiro[2.3]hexyl, azaspiro[3.3]heptyl, oxaazaspiro[3.3]heptyl,         thiaazaspiro[3.3]heptyl, oxaspiro[3.3]heptyl,         oxazaspiro[5.3]nonyl, oxazaspiro[4.3]octyl, azaspiro[4,5]decyl,         oxazaspiro[5.5]undecyl, diazaspiro[3.3]heptyl,         thiazaspiro[3.3]heptyl, thiazaspiro[4.3]octyl,         azaspiro[5.5]undecyl, or one of the further homologous scaffolds         such as spiro[3.4]-, spiro[4.4]-, spiro[2.4]-, spiro[2.5]-,         spiro[2.6]-, spiro[3.5]-, spiro[3.6]-, spiro[4.5]- and         spiro[4.6]-.     -   The term “a 6- to 10-membered fused heterocycloalkyl” means a         bicyclic, saturated heterocycle with 6, 7, 8, 9 or 10 ring atoms         in total, in which the two rings share two adjacent ring atoms,         which “fused heterocycloalkyl” contains one or two identical or         different ring heteroatoms from the series: N, O, S; it being         possible for said fused heterocycloalkyl group to be attached to         the rest of the molecule via any one of the carbon atoms or, if         present, a nitrogen atom.     -   Said fused heterocycloalkyl group is, for example,         azabicyclo[3.3.0]octyl, azabicyclo[4.3.0]nonyl,         diazabicyclo[43.0]nonyl, oxazabicyclo[4.3.0]nonyl,         thiazabicyclo[4.3.0]nonyl or azabicyclo[4.4.0]decyl.     -   The term “a 7- to 10-membered bridged heterocycloalkyl” means a         bicyclic, saturated heterocycle with 7, 8, 9 or 10 ring atoms in         total, in which the two rings share two common ring atoms which         are not adjacent, which “bridged heterocycloalkyl” contains one         or two identical or different ring heteroatoms from the series:         N, O, S; it being possible for said bridged heterocycloalkyl         group to be attached to the rest of the molecule via any one of         the carbon atoms, except the spiro carbon atom, or, if present,         a nitrogen atom.     -   Said bridged heterocycloalkyl group is, for example,         azabicyclo[2.2.1]heptyl, oxazabicyclo[2.2.1]heptyl,         thiazabicyclo[2.2.1]heptyl, diazabicyclo[2.2.1]heptyl,         azabicyclo[2.2.2]octyl, diazabicyclo[2.2.2]octyl,         oxazabicyclo[2.2.2]octyl, thiazabicyclo[2.2.2]octyl,         azabicyclo[3.2.1]octyl, diazabicyclo[3.2.1]octyl,         oxazabicyclo[3.2.1]octyl, thiazabicyclo[3.2.1]octyl,         azabicyclo[33.1]nonyl, diazabicyclo[3.3.1]nonyl,         oxazabicyclo[3.3.1]nonyl, thiazabicyclo[3.3.1]nonyl,         azabicyclo[4.2.1]nonyl, diazabicyclo[4.2.1]nonyl,         oxazabicyclo[4.2.1]nonyl, thiazabicyclo[4.2.1]nonyl,         azabicyclo[3.3.2]decyl, diazabicyclo[3.3.2]decyl,         oxazabicyclo[3.3.2]decyl, thiazabicyclo[3.3.2]decyl or         azabicyclo[4.2.2]decyl.

The term “bicyclic nitrogen containing 6-11 membered heterocycloalkyl” means a 6- to 11-membered heterospirocycloalkyl, 6- to 10-membered fused heterocycloalkyl or 7- to 10-membered bridged heterocycloalkyl group as defined supra, however containing one ring nitrogen atom and optionally one or two further ring heteroatoms from the series N, O and S; it being possible for said bicyclic nitrogen containing 6-11 membered heterocycloalkyl group to be attached to the rest of the molecule via a nitrogen atom or any one of the carbon atoms, except a spiro carbon atom.

The term “bicyclic 5-11 membered heterocycloalkyl” means a 5-11 membered heterospirocycloalkyl, a 5-11 membered fused heterocycloalkyl or a 5-11 membered bridged heterocycloalkyl group as defined below:

-   -   The term “5-11 membered heterospirocycloalkyl” means a bicyclic,         saturated heterocycle with 5, 6, 7, 8, 9, 10 or 11 ring atoms in         total, in which the two rings share one common ring carbon atom,         which “heterospirocycloalkyl” contains one or two identical or         different ring heteroatoms from the series: N, O, S; it being         possible for said heterospirocycloalkyl group to be attached to         the rest of the molecule via any one of the carbon atoms, except         the spiro carbon atom, or, if present, a nitrogen atom.     -   Said heterospirocycloalkyl group is, for example,         azaspiro[2.2]pentyl, azaspiro[2.3]hexyl, azaspiro[3.3]heptyl,         oxaazaspiro[3.3]heptyl, thiaazaspiro[3.3]heptyl,         oxaspiro[3.3]heptyl, oxazaspiro[5.3]nonyl, oxazaspiro[4.3]octyl,         azaspiro[4,5]decyl, oxazaspiro[5.5]undecyl,         diazaspiro[3.3]heptyl, thiazaspiro[3.3]heptyl,         thiazaspiro[4.3]octyl, azaspiro[5.5]undecyl, or one of the         further homologous scaffolds such as spiro[3.4]-, spiro[4.4]-,         spiro[2.4]-, spiro[2.5]-, spiro[2.6]-, spiro[3.5]-, spiro[3.6]-,         spiro[4.5]- and spiro[4.6]-.     -   The term “5-11 membered fused heterocycloalkyl” means a         bicyclic, saturated heterocycle with 5, 6, 7, 8, 9 or 10 ring         atoms in total, in which the two rings share two adjacent ring         atoms, which “fused heterocycloalkyl” contains one or two         identical or different ring heteroatoms from the series: N, O,         S; it being possible for said fused heterocycloalkyl group to be         attached to the rest of the molecule via any one of the carbon         atoms or, if present, a nitrogen atom.     -   Said fused heterocycloalkyl group is, for example,         azabicyclo[3.1.0]hexyl, azabicyclo[3.3.0]octyl,         azabicyclo[4.3.0]nonyl, diazabicyclo[4.3.0]nonyl,         oxazabicyclo[4.3.0]nonyl, thiazabicyclo[4.3.0]nonyl or         azabicyclo[4.4.0]decyl.     -   The term “5-11 membered bridged heterocycloalkyl” means a         bicyclic, saturated heterocycle with 5, 6, 7, 8, 9 or 10 ring         atoms in total, in which the two rings share two common ring         atoms which are not adjacent, which “bridged heterocycloalkyl”         contains one or two identical or different ring heteroatoms from         the series: N, O, S; it being possible for said bridged         heterocycloalkyl group to be attached to the rest of the         molecule via any one of the carbon atoms, except the spiro         carbon atom, or, if present, a nitrogen atom.     -   Said bridged heterocycloalkyl group is, for example,         azabicyclo[2.2.1]heptyl, oxazabicyclo[2.2.1]heptyl,         thiazabicyclo[2.2.1]heptyl, diazabicyclo[2.2.1]heptyl,         azabicyclo[2.2.2]octyl, diazabicyclo[2.2.2]octyl,         oxazabicyclo[2.2.2]octyl, thiazabicyclo[2.2.2]octyl,         azabicyclo[3.2.1]octyl, diazabicyclo[3.2.1]octyl,         oxazabicyclo[3.2.1]octyl, thiazabicyclo[3.2.1]octyl,         azabicyclo[3.3.1]nonyl, diazabicyclo[3.3.1]nonyl,         oxazabicyclo[3.3.1]nonyl, thiazabicyclo[3.3.1]nonyl,         azabicyclo[4.2.1]nonyl, diazabicyclo[4.2.1]nonyl,         oxazabicyclo[4.2.1]nonyl, thiazabicyclo[4.2.1]nonyl,         azabicyclo[3.3.2]decyl, diazabicyclo[3.3.2]decyl,         oxazabicyclo[3.3.2]decyl, thiazabicyclo[3.3.2]decyl or         azabicyclo[4.2.2]decyl.

The term “bicyclic nitrogen containing 5-11 membered heterocycloalkyl” means a 5-11 membered heterospirocycloalkyl, 5-11 membered fused heterocycloalkyl or 5-11 membered bridged heterocycloalkyl group as defined supra, however containing one ring nitrogen atom and optionally one or two further ring heteroatoms from the series N, O and S; it being possible for said bicyclic nitrogen containing 5-11 membered heterocycloalkyl group to be attached to the rest of the molecule via a nitrogen atom or any one of the carbon atoms, except a spiro carbon atom.

The term “heteroaryl” means a monovalent, monocyclic or bicyclic aromatic ring having 5, 6, 8, 9 or 10 ring atoms (a “5- to 10-membered heteroaryl” group), which contains at least one ring heteroatom and optionally one, two or three further ring heteroatoms from the series: N, O and/or S, and which is bound via a ring carbon atom, or, if valency allows as e.g. in pyrrol-1-yl, a nitrogen atom.

Said heteroaryl group can be a 5-membered heteroaryl group, such as, for example, thienyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl or tetrazolyl; or a 6-membered heteroaryl group, such as, for example, pyridinyl (herein also referred to as pyridyl), pyridazinyl, pyrimidinyl, pyrazinyl or triazinyl; or a 9-membered heteroaryl group, such as, for example, benzofuranyl, benzothienyl, benzoxazolyl, benzisoxazolyl, benzimidazolyl, benzothiazolyl, benzotriazolyl, thiazolopyridinyl, indazolyl, indolyl, isoindolyl, indolizinyl or purinyl; or a 10-membered heteroaryl group, such as, for example, quinolinyl, quinazolinyl, isoquinolinyl, cinnolinyl, phthalazinyl, quinoxalinyl or pteridinyl. In general, and unless otherwise mentioned, the heteroaryl or heteroarylene groups include all possible isomeric forms thereof, e.g.: tautomers and positional isomers with respect to the point of linkage to the rest of the molecule. Thus, for some illustrative non-restricting examples, the term pyridinyl includes pyridin-2-yl, pyridin-3-yl and pyridin-4-yl; or the term thienyl includes thien-2-yl and thien-3-yl.

The term “C₁-C₆”, as used in the present text, e.g. in the context of the definition of “C₁-C₆-alkyl”, “C₁-C₆-haloalkyl”, “C₁-C₆-hydroxyalkyl”, “C₁-C₆-alkoxy” or “C₁-C₆-haloalkoxy” means an alkyl group having a finite number of carbon atoms of 1 to 6, i.e. 1, 2, 3, 4, 5 or 6 carbon atoms.

Further, as used herein, the term “C₃-C₇”, as used in the present text, e.g. in the context of the definition of “C₃-C₇-cycloalkyl”, means a cycloalkyl group having a finite number of carbon atoms of 3 to 7, i.e. 3, 4, 5, 6 or 7 carbon atoms.

When a range of values is given, said range encompasses each value and sub-range within said range.

For example:

-   -   “C₁-C₆” encompasses C₁, C₂, C₃, C₄, C₅, C₆, C₁-C₆, C₁-C₅, C₁-C₄,         C₁-C₃, C₁-C₂, C₂- C₆, C₂-C₅, C₂-C₄, C₂-C₃, C₃-C₆, C₃-C₅, C₃-C₄,         C₄-C₆, C₄-C₅, and C₅-C₆;     -   “C₂-C₆” encompasses C₂, C₃, C₄, C₅, C₆, C₂-C₆, C₂-C₅, C₂-C₄,         C₂-C₃, C₃-C₆, C₃-C₆, C₃-C₄, C₄-C₆, C₄-C₅, and C₅-C₆;     -   “C₃-C₆” encompasses C₃, C₄, C₅, C₆, C₃-C₆, C₃-C₅, C₃-C₄, C₄-C₆,         C₄-C₅, and C₅-C₆.

As used herein, the term “leaving group” means an atom or a group of atoms that is displaced in a chemical reaction as stable species taking with it the bonding electrons. In particular, such a leaving group is selected from the group comprising: a halogen atom, in particular a fluorine atom, a chlorine atom, a bromine atom or an iodide atom, being displaced as halide, in particular fluoride, chloride, bromide or iodide; (methylsulfonyl)oxy, [(trifluoromethyl)sulfonyl]oxy, [(nonafluorobutyl)sulfonyl]oxy, (phenylsulfonyl)oxy, [(4-methylphenyl)sulfonyl]oxy, [(4-bromophenyl)sulfonyl]oxy, [(4-nitrophenyl)sulfonyl]oxy, [(2-nitrophenyl)sulfonyl]oxy, [(4-isopropylphenyl)sulfonyl]oxy, [(2,4,6-triisopropylphenyl)sulfonyl]oxy, [(2,4,6-trimethylphenyl)sulfonyl]oxy, [(4-tert-butylphenyl)sulfonyl]oxy and [(4-methoxyphenyl)sulfonyl]oxy.

As used herein, the term “dipolar aprotic solvent” means a solvent selected from acetone, acetonitrile, propionitrile, dimethylsulfoxide, diethylsulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N,N-diethylformamide, N,N-diethylacetamide, 1-methyl-2-pyrrolidinone, 1-ethyl-2-pyrrolidinone, 1-methyl-2-piperidinone and 1-ethyl-2-piperidinone, or mixtures thereof.

Particularly, said dipolar aprotic solvent is acetonitrile, dimethylsulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide or 1-methyl-2-pyrrolidinone.

As used herein, the term “room temperature” means a temperature in the range from 15° C. to 25° C.

It is possible for the compounds of general formula (I) to exist as isotopic variants. The invention therefore includes one or more isotopic variant(s) of the compounds of general formula (I), particularly deuterium-containing compounds of general formula (I).

The term “Isotopic variant” of a compound or a reagent is defined as a compound exhibiting an unnatural proportion of one or more of the isotopes that constitute such a compound.

The term “Isotopic variant of the compound of general formula (I)” is defined as a compound of general formula (I) exhibiting an unnatural proportion of one or more of the isotopes that constitute such a compound.

The expression “unnatural proportion” means a proportion of such isotope which is higher than its natural abundance. The natural abundances of isotopes to be applied in this context are described in “Isotopic Compositions of the Elements 1997”, Pure Appl. Chem., 70(1), 217-235, 1998.

Examples of such isotopes include stable and radioactive isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine, chlorine, bromine and iodine, such as ²H (deuterium), ³H (tritium), ¹¹C, ¹³C, ¹⁴C, ¹⁵N, ¹⁷O, ¹⁸O, ³²P, ³³P, ³³S, ³⁴S, ³⁵S, ³⁶S, ¹⁸F, ³⁶Cl, ⁸²Br, ¹²³I, ¹²⁴I, ¹²⁵I, ¹²⁹I and ¹³¹I, respectively.

With respect to the treatment and/or prophylaxis of the disorders specified herein the isotopic variant(s) of the compounds of general formula (I) preferably contain deuterium (“deuterium-containing compounds of general formula (I)”). Isotopic variants of the compounds of general formula (I) in which one or more radioactive isotopes, such as ³H or ¹⁴C, are incorporated are useful e.g. in drug and/or substrate tissue distribution studies. These isotopes are particularly preferred for the ease of their incorporation and detectability. Positron emitting isotopes such as ¹⁸F or ¹¹C may be incorporated into a compound of general formula (I). These isotopic variants of the compounds of general formula (I) are useful for in vivo imaging applications. Deuterium-containing and ¹³C-containing compounds of general formula (I) can be used in mass spectrometry analyses in the context of preclinical or clinical studies.

Isotopic variants of the compounds of general formula (I) can generally be prepared by methods known to a person skilled in the art, such as those described in the schemes and/or examples herein, by substituting a reagent for an isotopic variant of said reagent, preferably for a deuterium-containing reagent. Depending on the desired sites of deuteration, in some cases deuterium from D₂O can be incorporated either directly into the compounds or into reagents that are useful for synthesizing such compounds. Deuterium gas is also a useful reagent for incorporating deuterium into molecules. Catalytic deuteration of olefinic bonds and acetylenic bonds is a rapid route for incorporation of deuterium. Metal catalysts (i.e. Pd, Pt, and Rh) in the presence of deuterium gas can be used to directly exchange deuterium for hydrogen in functional groups containing hydrocarbons. A variety of deuterated reagents and synthetic building blocks are commercially available from companies such as for example C/D/N Isotopes, Quebec, Canada; Cambridge Isotope Laboratories Inc., Andover, Mass., USA; and CombiPhos Catalysts, Inc., Princeton, N.J., USA.

The term “deuterium-containing compound of general formula (I)” is defined as a compound of general formula (I), in which one or more hydrogen atom(s) is/are replaced by one or more deuterium atom(s) and in which the abundance of deuterium at each deuterated position of the compound of general formula (I) is higher than the natural abundance of deuterium, which is about 0.015%. Particularly, in a deuterium-containing compound of general formula (I) the abundance of deuterium at each deuterated position of the compound of general formula (I) is higher than 10%, 20%, 30%, 40%, 50%, 60%, 70% or 80%, preferably higher than 90%, 95%, 96% or 97%, even more preferably higher than 98% or 99% at said position(s). It is understood that the abundance of deuterium at each deuterated position is independent of the abundance of deuterium at other deuterated position(s).

The selective incorporation of one or more deuterium atom(s) into a compound of general formula (I) may alter the physicochemical properties (such as for example acidity [C. L. Perrin, et al., J. Am. Chem. Soc., 2007, 129, 4490], basicity [C. L. Perrin et al., J. Am. Chem. Soc., 2005, 127, 9641], lipophilicity [B. Testa et al., Int. J. Pharm., 1984, 19(3), 271]) and/or the metabolic profile of the molecule and may result in changes in the ratio of parent compound to metabolites or in the amounts of metabolites formed. Such changes may result in certain therapeutic advantages and hence may be preferred in some circumstances. Reduced rates of metabolism and metabolic switching, where the ratio of metabolites is changed, have been reported (A. E. Mutlib et al., Toxicol. Appl. Pharmacol., 2000, 169, 102). These changes in the exposure to parent drug and metabolites can have important consequences with respect to the pharmacodynamics, tolerability and efficacy of a deuterium-containing compound of general formula (I). In some cases deuterium substitution reduces or eliminates the formation of an undesired or toxic metabolite and enhances the formation of a desired metabolite (e.g. Nevirapine: A. M. Sharma et al., Chem. Res. Toxicol., 2013, 26, 410; Efavirenz: A. E. Mutlib et al., Toxicol. Appl. Pharmacol., 2000, 169, 102). In other cases the major effect of deuteration is to reduce the rate of systemic clearance. As a result, the biological half-life of the compound is increased. The potential clinical benefits would include the ability to maintain similar systemic exposure with decreased peak levels and increased trough levels. This could result in lower side effects and enhanced efficacy, depending on the particular compound's pharmacokinetic/pharmacodynamic relationship. ML-337 (C. J. Wenthur et al., J. Med. Chem., 2013, 56, 5208) and Odanacatib (K. Kassahun et al., WO2012/112363) are examples for this deuterium effect. Still other cases have been reported in which reduced rates of metabolism result in an increase in exposure of the drug without changing the rate of systemic clearance (e.g. Rofecoxib: F. Schneider et al., Arzneim. Forsch./Drug. Res., 2006, 56, 295; Telaprevir: F. Maltais et al., J. Med. Chem., 2009, 52, 7993). Deuterated drugs showing this effect may have reduced dosing requirements (e.g. lower number of doses or lower dosage to achieve the desired effect) and/or may produce lower metabolite loads.

A compound of general formula (I) may have multiple potential sites of attack for metabolism. To optimize the above-described effects on physicochemical properties and metabolic profile, deuterium-containing compounds of general formula (I) having a certain pattern of one or more deuterium-hydrogen exchange(s) can be selected. Particularly, the deuterium atom(s) of deuterium-containing compound(s) of general formula (I) is/are attached to a carbon atom and/or is/are located at those positions of the compound of general formula (I), which are sites of attack for metabolizing enzymes such as e.g. cytochrome P₄₅₀.

In another embodiment the present invention concerns a deuterium-containing compound of general formula (I) having 1, 2, 3 or 4 deuterium atoms, particularly with 1, 2 or 3 deuterium atoms.

Where the plural form of the word compounds, salts, polymorphs, hydrates, solvates and the like, is used herein, this is taken to mean also a single compound, salt, polymorph, isomer, hydrate, solvate or the like.

By “stable compound” or “stable structure” is meant a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent.

The compounds of the present invention optionally contain one or more asymmetric centres, depending upon the location and nature of the various substituents desired. It is possible that one or more asymmetric carbon atoms are present in the (R) or (S) configuration, which can result in racemic mixtures in the case of a single asymmetric centre, and in diastereomeric mixtures in the case of multiple asymmetric centres. In certain instances, it is possible that asymmetry also be present due to restricted rotation about a given bond, for example, the central bond adjoining two substituted aromatic rings of the specified compounds.

Preferred isomers are those which produce the more desirable biological activity. These separated, pure or partially purified isomers or racemic mixtures of the compounds of this invention are also included within the scope of the present invention. The purification and the separation of such materials can be accomplished by standard techniques known in the art.

The optical isomers can be obtained by resolution of the racemic mixtures according to conventional processes, for example, by the formation of diastereoisomeric salts using an optically active acid or base or formation of covalent diastereomers. Examples of appropriate acids are tartaric, diacetyltartaric, ditoluoyltartaric and camphorsulfonic acid. Mixtures of diastereoisomers can be separated into their individual diastereomers on the basis of their physical and/or chemical differences by methods known in the art, for example, by chromatography or fractional crystallisation. The optically active bases or acids are then liberated from the separated diastereomeric salts. A different process for separation of optical isomers involves the use of chiral chromatography (e.g., HPLC columns using a chiral phase), with or without conventional derivatisation, optimally chosen to maximise the separation of the enantiomers. Suitable HPLC columns using a chiral phase are commercially available, such as those manufactured by Daicel, e.g., Chiracel OD and Chiracel OJ, for example, among many others, which are all routinely selectable. Enzymatic separations, with or without derivatisation, are also useful. The optically active compounds of the present invention can likewise be obtained by chiral syntheses utilizing optically active starting materials.

In order to distinguish different types of isomers from each other reference is made to IUPAC Rules Section E (Pure Appl Chem 45, 11-30, 1976).

The present invention includes all possible stereoisomers of the compounds of the present invention as single stereoisomers, or as any mixture of said stereoisomers, e.g. (R)- or (S)-isomers, in any ratio. Isolation of a single stereoisomer, e.g. a single enantiomer or a single diastereomer, of a compound of the present invention is achieved by any suitable state of the art method, such as chromatography, especially chiral chromatography, for example.

Further, it is possible for some of the compounds of the present invention to exist as tautomers. For example, the compounds of the present invention may contain a pyridone moiety and can exist as a pyridone, or as an hydroxypyridine, or even a mixture in any amount of the two tautomers, namely:

The present invention includes all possible tautomers of the compounds of the present invention as single tautomers, or as any mixture of said tautomers, in any ratio.

Further, the compounds of the present invention can exist as N-oxides, which are defined in that at least one nitrogen of the compounds of the present invention is oxidised. The present invention includes all such possible N-oxides.

The present invention also covers useful forms of the compounds of the present invention, such as metabolites, hydrates, solvates, prodrugs, salts, in particular pharmaceutically acceptable salts, and/or co-precipitates.

The compounds of the present invention can exist as a hydrate, or as a solvate, wherein the compounds of the present invention contain polar solvents, in particular water, methanol or ethanol for example, as structural element of the crystal lattice of the compounds. It is possible for the amount of polar solvents, in particular water, to exist in a stoichiometric or non-stoichiometric ratio. In the case of stoichiometric solvates, e.g. a hydrate, hemi-, (semi-), mono-, sesqui-, di-, tri-, tetra-, penta- etc. solvates or hydrates, respectively, are possible. The present invention includes all such hydrates or solvates.

Further, it is possible for the compounds of the present invention to exist in free form, e.g. as a free base, or as a free acid, or as a zwitterion, or to exist in the form of a salt. Said salt may be any salt, either an organic or inorganic addition salt, particularly any pharmaceutically acceptable organic or inorganic addition salt, which is customarily used in pharmacy, or which is used, for example, for isolating or purifying the compounds of the present invention. The term “pharmaceutically acceptable salt” refers to an inorganic or organic acid addition salt of a compound of the present invention. For example, see S. M. Berge et al., “Pharmaceutical Salts,” J. Pharm. Sci. 1977, 66, 1-19.

A suitable pharmaceutically acceptable salt of the compounds of the present invention may be, for example, an acid-addition salt of a compound of the present invention bearing a nitrogen atom, in a chain or in a ring, for example, which is sufficiently basic, such as an acid-addition salt with an inorganic acid, or “mineral acid”, such as hydrochloric, hydrobromic, hydroiodic, sulfuric, sulfamic, bisulfuric, phosphoric, or nitric acid, for example, or with an organic acid, such as formic, acetic, acetoacetic, pyruvic, trifluoroacetic, propionic, butyric, hexanoic, heptanoic, undecanoic, lauric, benzoic, salicylic, 2-(4-hydroxybenzoyl)-benzoic, camphoric, cinnamic, cyclopentanepropionic, digluconic, 3-hydroxy-2-naphthoic, nicotinic, pamoic, pectinic, 3-phenylpropionic, pivalic, 2-hydroxyethanesulfonic, itaconic, trifluoromethanesulfonic, dodecylsulfuric, ethanesulfonic, benzenesulfonic, para-toluenesulfonic, methanesulfonic, 2-naphthalenesulfonic, naphthalenedisulfonic, camphorsulfonic acid, citric, tartaric, stearic, lactic, oxalic, malonic, succinic, malic, adipic, alginic, maleic, fumaric, D-gluconic, mandelic, ascorbic, glucoheptanoic, glycerophosphoric, aspartic, sulfosalicylic, or thiocyanic acid, for example.

Further, another suitably pharmaceutically acceptable salt of a compound of the present invention which is sufficiently acidic, is an alkali metal salt, for example a sodium or potassium salt, an alkaline earth metal salt, for example a calcium, magnesium or strontium salt, or an aluminium or a zinc salt, or an ammonium salt derived from ammonia or from an organic primary, secondary or tertiary amine having 1 to 20 carbon atoms, such as ethylamine, diethylamine, triethylamine, ethyldiisopropylamine, monoethanolamine, diethanolamine, triethanolamine, dicyclohexylamine, dimethylaminoethanol, diethylaminoethanol, tris(hydroxymethyl)aminomethane, procaine, dibenzylamine, N-methylmorpholine, arginine, lysine, 1,2-ethylenediamine, N-methylpiperidine, N-methyl-glucamine, N,N-dimethyl-glucamine, N-ethyl-glucamine, 1,6-hexanediamine, glucosamine, sarcosine, serinol, 2-amino-1,3-propanediol, 3-amino-1,2-propanediol, 4-amino-1,2,3-butanetriol, or a salt with a quaternary ammonium ion having 1 to 20 carbon atoms, such as tetramethylammonium, tetraethylammonium, tetra(n-propyl)ammonium, tetra(n-butyl)ammonium, N-benzyl-N,N,N-trimethylammonium, choline or benzalkonium.

Those skilled in the art will further recognise that it is possible for acid addition salts of the claimed compounds to be prepared by reaction of the compounds with the appropriate inorganic or organic acid via any of a number of known methods. Alternatively, alkali and alkaline earth metal salts of acidic compounds of the present invention are prepared by reacting the compounds of the present invention with the appropriate base via a variety of known methods.

The present invention includes all possible salts of the compounds of the present invention as single salts, or as any mixture of said salts, in any ratio.

In the present text, in particular in the “Experimental Section”, for the synthesis of intermediates and of examples of the present invention, when a compound is mentioned as a salt form with the corresponding base or acid, the exact stoichiometric composition of said salt form, as obtained by the respective preparation and/or purification process, is, in most cases, unknown.

Unless specified otherwise, suffixes to chemical names or structural formulae relating to salts, such as “hydrochloride”, “trifluoroacetate”, “sodium salt”, or “×HCl”, “×CF₃COOH”, “×Na”, for example, mean a salt form, the stoichiometry of which salt form not being specified.

This applies analogously to cases in which synthesis intermediates or example compounds or salts thereof have been obtained, by the preparation and/or purification processes described, as solvates, such as hydrates, with (if defined) unknown stoichiometric composition.

Furthermore, the present invention includes all possible crystalline forms, or polymorphs, of the compounds of the present invention, either as single polymorph, or as a mixture of more than one polymorph, in any ratio.

Moreover, the present invention also includes prodrugs of the compounds according to the invention. The term “prodrugs” here designates compounds which themselves can be biologically active or inactive, but are converted (for example metabolically or hydrolytically) into compounds according to the invention during their residence time in the body.

The invention further includes all possible cyclodextrin clathrates, i.e. alpha-, beta-, or gamma-cyclodextrins, hydroxypropyl-beta-cyclodextrins, methylbetacyclodextrins.

In accordance with a second embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from hydroxy, cyano, nitro, C₁-C₄-alkyl,         (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl, C₁-C₄-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₄-haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted one or         two times, each substituent independently selected from a         halogen atom or a group selected from cyano, C₁-C₂-alkyl, and         C₁-C₂-alkoxy;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         (5-membered         heteroaryl)-(C₁-C₂-alkyl)-(C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)-,         ((R⁹)O)—(C₁-C₄-alkyl)-, C₁-C₄-haloalkyl, C₃-C₇-cycloalkyl, —OR⁹,         —N(R¹⁰)(R¹¹), ((R¹⁰)(R¹¹)N)—(C₁-C₃-alkyl)-, —C(═O)—N(R¹²)(R¹³),         S(═O)_(n)—R¹⁴, —C(═O)R¹⁴, —C(═O)—OR¹⁷, and a 5-membered         heteroaryl group which itself is optionally substituted with one         or two methyl groups,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—;     -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from oxo, hydroxy, C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl,         C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-, —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²¹)(R²¹), —C(═O)—N(R²¹)(R²¹) and phenyl,         -   wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-group and said phenyl group itself             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a bromine atom, or a group selected from             cyano, methyl, trifluoromethyl and methoxy;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl, C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((R²²)(R²³)N)—C₂-alkyl, (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₇-cycloalkyl,         (C₃-C₇-cycloalkyl)-C(═O)—, (phenyl)-(C₁-C₂-alkyl)-,         (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₇-cycloalkyl within             said (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)- and             (C₃-C₇-cycloalkyl)-C(═O)— groups are optionally substituted             one or two times, each substituent independently selected             from a fluorine atom or a group selected from cyano,             C₁-C₂-alkyl and C₁-C₂-haloalkyl,         -   and wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 5- to 10-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₂-alkyl, C₁-C₂-haloalkyl,         (C₁-C₂-alkyl)-C(═O)—, C₁-C₂-alkoxy, —N(R²²)(R²³), and a         monocyclic 4- to 7-membered heterocycloalkyl group;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₄-haloalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             halogen atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₃-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from cyano, methyl, trifluoromethyl and             methoxy, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—;     -   R¹⁴ represents a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl and phenyl,         -   wherein the phenyl group is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy:     -   R¹⁷ represents a C₁-C₄-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group;     -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 5- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl, adamantyl,             monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 5- to             10-membered heterocycloalkyl, phenyl, and 5- to 10-membered             heteroaryl, said phenyl and 5- to 10-membered heteroaryl             substituents themselves being optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,         -   and wherein C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 5- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, and     -   n represents an integer 0, 1, or 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a third embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from hydroxy, cyano,         C₁-C₄-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₂-fluoroalkoxy and —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted with one         methyl group;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, C₁-C₃-alkyl, ((R⁹)O)—(C₁-C₃-alkyl)-,         C₁-C₃-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³),         S(═O)_(n)—R⁴ and —C(═O)—OR¹⁷,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—;     -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from hydroxy         and C₁-C₂-alkyl;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl, C₂-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷, —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹),         —C(═O)—N(R²³)(R²¹) and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-(C₁-C₂)-alkyl)-C(═O)—,         C₃-C₇-cycloalkyl, C₃-C₇-cycloalkyl-(C═O)—,         (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)- and the             C₃-C₇-cycloalkyl within the C₃-C₇-cycloalkyl-(C═O)— groups             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom or a             group selected from cyano, C₁-C₂-alkyl and             C₁-C₂-fluoroalkyl,         -   and wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         oxo, C₁-C₂-alkyl, C₁-C₂-fluoroalkyl and (C₁-C₂)-alkyl)-C(═O)—;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-fluoroalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—;         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—;     -   R¹⁴ represents a group selected from methyl and trifluoromethyl;     -   R¹⁷ represents a C₁-C₂-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a methyl group;     -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   and wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic             4- to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-fluoroalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, and     -   n represents an integer 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a fourth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from cyano, methyl, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy, or two substituents attached to adjacent         carbon atoms of said phenyl or pyridinyl group together form a         bivalent group —O—CF₂—O—;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a halogen atom or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and —C(═O)—OR¹⁷;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹), —C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-group are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom or a group selected from             cyano, methyl and C₁-fluoroalkyl,         -   and wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— group is optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and C₁-fluoroalkyl;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-C₃-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇-cycloalkyl is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom or a methyl group,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy;     -   R¹⁷ represents a C₁-C₂-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a methyl group;     -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₃-alkyl and phenyl,         -   wherein said C₁-C₅-alkyl group is optionally substituted one             or two times, each substituent independently selected from a             fluorine atom or a group selected from hydroxy, C₁-C₃-alkoxy             and phenyl, said phenyl itself being optionally substituted             one or two times, each substituent independently selected             from a fluorine atom, a chlorine atom and a methyl group,         -   and wherein said phenyl group is optionally substituted one,             two or three times, each substituent independently selected             from a fluorine atom and a chlorine atom or a group selected             from methyl, trifluoromethyl, methoxy and trifluoromethoxy,             and     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a fifth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from cyano, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group —O—CF₂—O—;     -   R² represents a group

-   -   -   wherein “*” indicates the point of attachment to the             nitrogen atom to which R² is attached;

    -   R³ represents a group selected from methyl and —NH₂;

    -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a halogen atom or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and —C(═O)—OR¹⁷;

    -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;

    -   R⁸ represents a —C(═O)—NH₂ group;

    -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹), —C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl;

    -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-group are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom or a group selected from             cyano, methyl and C₁-fluoroalkyl,         -   and wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— group is optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or

    -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and C-fluoroalkyl;

    -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-C₃-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇cycloalkyl is optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a methyl group,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy;

    -   R¹⁷ represents a C₁-C₂-alkyl group;

    -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a methyl group;

    -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₃-alkyl and phenyl,         -   wherein said C₁-C₃-alkyl group is optionally substituted one             or two times, each substituent independently selected from a             fluorine atom or a group selected from hydroxy, C₁-C₃-alkoxy             and phenyl, said phenyl itself being optionally substituted             one or two times, each substituent independently selected             from a fluorine atom, a chlorine atom and a methyl group,         -   and wherein said phenyl group is optionally substituted one,             two or three times, each substituent independently selected             from a fluorine atom and a chlorine atom or a group selected             from methyl, trifluoromethyl, methoxy and trifluoromethoxy,             and

    -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,

    -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a sixth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;

R³ represents a group selected from methyl and —NH₂;

R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹), —C(═O)—N(R²⁰)(R²¹) and phenyl,         wherein the phenyl group within said benzyl group and said         phenyl group itself are optionally substituted one or two times,         each substituent independently selected from a fluorine atom and         a chlorine atom, or a group selected from cyano and methyl;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₃-C₇-cycloalkyl and (benzyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom or a group selected from methyl and             trifluoromethyl,         -   and wherein the phenyl group within said (benzyl)-O—C(═O)—             group is optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a methyl group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and trifluoromethyl;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         (C₁-C₂-fluoroalkoxy)-C₂-alkyl-, (phenoxy)-C₂-alkyl-,         C₃-C₇-cycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein the phenyl groups within said             (phenoxy)-C₂-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy;     -   R¹⁷ represents a C₁-C₂-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a methyl group;     -   R²⁰ represents a group selected from benzyl and phenyl,         -   wherein said phenyl group, and the phenyl group within said             benzyl group, is optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,     -   R²¹ represents a hydrogen atom or a methyl group,     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy, and     -   R²⁷ represents a halogen atom or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and —C(═O)—OR¹⁷,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a seventh embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (benzyl)-O—C(═O)—, and wherein the phenyl group within said         (benzyl)-O—C(═O)— group is optionally substituted one or two         times, each substituent independently selected from a fluorine         atom, a chlorine atom and a methyl group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and trifluoromethyl;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         (C₁-C₄-alkoxy)-C₂-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-alkyl-,         (phenoxy)-C₂-alkyl-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═ or —N═;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from difluoromethyl, methoxy,         benzyloxy, difluoromethoxy and trifluoromethoxy, and     -   R²⁷ represents a halogen atom or a group selected from —OR⁹,         —N(R¹⁰)(R¹¹) and —C(═O)—N(R¹²)(R¹³),     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with an eighth embodiment of the first aspect, the present invention covers compounds of general formula (I):

-   -   in which:     -   R¹ represents a phenyl or 6-membered heteroaryl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₆-alkyl, (phenyl)-(C₁-C₃-alkyl)-,         C₁-C₆-haloalkyl, C₁-C₆-alkoxy, (phenyl)-(C₁-C₃-alkoxy)-,         C₁-C₆-haloalkoxy, —N(R⁵)(R⁶);         -   wherein the phenyl groups in said (phenyl)-(C₁-C₃-alkyl)-             and (phenyl)-(C₁-C₃-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a halogen atom or a group selected from cyano,             methyl, ethyl, trifluoromethyl, methoxy, ethoxy,             dimethylamino and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or 6-membered heteroaryl group together form a             bivalent group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —(CH₂)₂—O—CH₂—, —O—CH₂—O—,             —O—CH₂—CH₂—O—, —O—CF₂—O—, —O—CH₂—CF₂—O—, and —O—CF₂—CF₂—O—,             or     -   R¹ represents a 5-membered heteroaryl group optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from cyano,         C₁-C₃-alkyl, and C₁-C₃-alkoxy;

R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or 6-membered heteroaryl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₆-alkyl, (phenyl)-(C₁-C₃-alkyl)-,         (5-membered heteroaryl)-(C₁-C₃-alkyl)-, C₁-C₆-hydroxyalkyl,         C₁-C₆-haloalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³),         S(═O)_(n)—R¹⁴, and a 5-membered heteroaryl group which itself is         optionally substituted with one or two methyl groups,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or 6-membered heteroaryl group together form a             bivalent group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —(CH₂)₂—O—CH₂—, —O—CH₂—O—,             —O—CH₂—CH₂—O—, —O—CF₂—O—, —O—CH₂—CF₂—O—, and —O—CF₂—CF₂—O—;     -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl and         (phenyl)-(C₁-C₃-alkyl)-, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4-to         7-membered heterocycloalkyl group which is optionally         substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from oxo, hydroxy, C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl and C₁-C₄-alkoxy;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a group selected from —C(═O)—NH₂ and —S(═O)₂—NH₂;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, (phenyl)-(C₁-C₃-alkyl)-, C₁-C₆-haloalkyl,         C₂-C₄-hydroxyalkyl, (C₁-C₃-alkoxy)-C₂-C₃-alkyl-,         ((C₁-C₃-alkyl)-C(═O)—O)—C₂-C₃-alkyl-, —C(R¹⁸)(R¹⁹)—C(═O)—OR¹,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said             (phenyl)-(C₁-C₃-alkyl)-group and said phenyl group itself             are optionally substituted one or two times, each             substituent independently selected from a halogen atom or a             group selected from cyano, methyl, ethyl, trifluoromethyl,             methoxy, ethoxy, dimethylamino and trifluoromethoxy;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₃-alkyl,         C₁-C₄-haloalkyl, C₂-C₄-hydroxyalkyl,         (C₁-C₃-alkoxy)-C₂-C₃-alkyl-, ((R²²)(R²³)N)—C₂-C₃-alkyl,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₇-cycloalkyl, (C₃-C₇-cycloalkyl)         —C(═O)—, (phenyl)-(C₁-C₃-alkyl)-, (phenyl)-(C₁-C₃-alkyl)-C(═O)—         and (phenyl)-(C₁-C₃-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₃-alkyl)-, (phenyl)-(C₁-C₃-alkyl)-C(═O)— and             (phenyl)-(C₁-C₃-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a halogen atom or a group selected from cyano,             methyl, ethyl, trifluoromethyl, methoxy, ethoxy,             dimethylamino and trifluoromethoxy, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 6- to 11-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₄-alkyl, C₁-C₄-haloalkyl,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₇-cycloalkyl, C₁-C₄-alkoxy,         —N(R²²)(R²³), and a monocyclic 4- to 7-membered heterocycloalkyl         group;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₆-haloalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₃-alkoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from oxo, hydroxy,             C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl and             C₁-C₄-alkoxy, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from oxo, hydroxy, C₁-C₄-alkyl, (C₁-C₄-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl and C₁-C₄-alkoxy;     -   R¹⁴ represents a group selected from C₁-C₄-alkyl and         C₁-C₄-haloalkyl;     -   R¹⁷ represents a C₁-C₄-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₄-alkyl group;     -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to         11-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,             monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to             11-membered heterocycloalkyl, phenyl, and 5- to 10-membered             heteroaryl, said phenyl and 5- to 10-membered heteroaryl             substituents themselves being optionally substituted one or             two times, each substituent independently selected from a             halogen atom or a group selected from cyano, methyl, ethyl,             trifluoromethyl, methoxy, ethoxy, dimethylamino and             trifluoromethoxy,         -   wherein C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl and             bicyclic 6- to 11-membered heterocycloalkyl are optionally             substituted one, two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₆-alkyl,             (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl and C₁-C₄-alkoxy,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₄-alkyl,             C₁-C₄-haloalkyl, C₁-C₄-alkoxy, C₁-C₄-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵)     -   R²¹ represents a hydrogen atom or a C₁-C₄-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₄-alkyl, C₁-C₄-haloalkyl, (phenyl)-(C₁-C₃-alkyl)-,         (C₁-C₄-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₄-alkoxy,         C₁-C₃-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²³ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₄-alkyl group, and     -   n represents an integer 0, 1, or 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a ninth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         C₁-C₄-haloalkyl, C₁-C₄-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-,         C₁-C₄-haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted one or         two times, each substituent independently selected from a         halogen atom or a group selected from cyano, C₁-C₂-alkyl, and         C₁-C₂-alkoxy;

R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         (5-membered heteroaryl)-(C₁-C₂-alkyl)-, C₁-C₄-hydroxyalkyl,         C₁-C₄-haloalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³),         S(═O)_(n)—R¹⁴, and a 5-membered heteroaryl group which itself is         optionally substituted with one or two methyl groups,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—;     -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from oxo, hydroxy, C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl,         C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-, —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-group and said phenyl group itself             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a bromine atom, or a group selected from             methyl, trifluoromethyl and methoxy;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl, C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((R²²)(R²³)N)—C₂-alkyl, (C₁-C₂-alkyl)-C(═O)—, C₃-C₅-cycloalkyl,         (C₃-C₅-cycloalkyl)-C(═O)—, (phenyl)-(C₁-C₂-alkyl)-,         (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 6- to 10-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₂-alkyl, C₁-C₂-haloalkyl,         (C₁-C₂-alkyl)-C(═O)—, C₁-C₂-alkoxy, —N(R²²)(R²³), and a         monocyclic 4- to 7-membered heterocycloalkyl group;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₆-haloalkyl, C₁-C₆-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             halogen atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—;     -   R¹⁴ represents a group selected from C₁-C₂-alkyl and         C₁-C₂-haloalkyl;     -   R¹⁷ represents a C₁-C₄-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group;     -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,             monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to             10-membered heterocycloalkyl, phenyl, and 5- to 10-membered             heteroaryl, said phenyl and 5- to 10-membered heteroaryl             substituents themselves being optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,         -   wherein C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 6- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵)     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, and     -   n represents an integer 0, 1, or 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a tenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from cyano, C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted with one         methyl group,     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from cyano, C₁-C₃-alkyl,         C₁-C₃-hydroxyalkyl, C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and S(═O)_(n)—R¹⁴,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—;     -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from hydroxy         and C₁-C₂-alkyl;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷, —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹) and         phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group;     -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkyl)-C(═O)—,         (phenyl)-(C₁-C₂-alkyl)-(phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from oxo,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl and (C₁-C₂-alkyl)-C(═O)—;     -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-fluoroalkyl, C₃-C₇-cycloalkyl, monocyclic 4- to 7-membered         heterocycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—;     -   R¹⁴ represents a group selected from methyl and trifluoromethyl;     -   R¹⁷ represents a C₁-C₂-alkyl group;     -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a methyl group;     -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic 4-             to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—, and             wherein said phenyl and 5- to 10-membered heteroaryl groups             are optionally substituted one, two or three times, each             substituent independently selected from a fluorine atom and             a chlorine atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, and     -   n represents an integer 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with an eleventh embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from cyano, C₁-C₂-alkyl, C₁-C₂-fluoroalkyl,         C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group, and     -   R⁹ represents a group selected from C₁-C₂-alkyl, benzyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkoxy)-C₂-alkyl- and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twelfth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from cyano, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         C₁-C₂-fluoroalkoxy;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R³ represents a —C(═O)—NH₂ group, and     -   R⁹ represents a group selected from C₁-C₂-alkyl, benzyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkoxy)-C₂-alkyl- and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group; and             stereoisomers, tautomers, N-oxides, hydrates, solvates, and             salts thereof, and mixtures of same.

In accordance with a thirteenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   Y² represents —C(H)═ and —N═;     -   Y³ represents —C(R²⁷)═ and —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy, and     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a fourteenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   -   wherein “*” indicates the point of attachment to the             nitrogen atom to which R² is attached;

    -   R³ represents a group selected from methyl and —NH₂;

    -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents N═, Y represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy, and     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a fifteenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═ or —N═;     -   Y² represents —C(H)═ and —N═;     -   Y³ represents —C(R²⁷)═ and —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents N═, Y^(represents —C(H)═;)     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from methyl, difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy, and     -   R²⁷ represents a chlorine atom, or a group selected from         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy, and         stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a sixteenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group,

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═ or —N═;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from methyl, difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy, and     -   R²⁷ represents a chlorine atom, or a group selected from         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy, and         stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a seventeenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a —NH₂ group;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   Y² represents —C(H)═ and —N═;     -   Y³ represents —C(R²⁷)═ and —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy, and     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with an eighteenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a —NH₂ group;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy, and     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a nineteenth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a —NH₂ group;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═ or —N═;     -   Y² represents —C(H)═ and —N═;     -   Y³ represents —C(R²⁷)═ and —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from methyl, difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy, and     -   R²⁷ represents a chlorine atom, or a group selected from         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy, and         stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twentieth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a —NH₂ group;     -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═, —C(F)═ or —N═;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from methyl, difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy, and     -   R²⁷ represents a chlorine atom, or a group selected from         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy, and         stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twenty-first embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached, or     -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a group selected from methyl and —NH₂;     -   R⁴ represents a group selected from

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═ or —C(F)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy;     -   R²⁸ represents a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, difluoromethoxy and trifluoromethoxy;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twenty-second embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached, or     -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a —NH₂ group;     -   R⁴ represents a group selected from

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═ or —C(F)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy;     -   R²⁸ represents a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, difluoromethoxy and trifluoromethoxy;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twenty-third embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached, or     -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a methyl group;     -   R⁴ represents a group selected from

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   Y¹ represents —C(H)═ or —C(F)═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy;     -   R²⁸ represents a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, difluoromethoxy and trifluoromethoxy;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twenty-fourth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group selected from

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a —NH₂ group;     -   R⁴ represents a group selected from

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In accordance with a twenty-fifth embodiment of the first aspect, the present invention covers compounds of general formula (I), supra, in which:

-   -   R¹ represents a group selected from

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R³ represents a methyl group;     -   R⁴ represents a group selected from

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

FURTHER EMBODIMENTS OF THE FIRST ASPECT OF THE PRESENT INVENTION

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from hydroxy, cyano, nitro, C₁-C₄-alkyl,         (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl, C₁-C₄-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₄-haloalkoxy, —N(R⁵)(R⁶)₁         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted one or         two times, each substituent independently selected from a         halogen atom or a group selected from cyano, C₁-C₂-alkyl, and         C₁-C₂-alkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from hydroxy, cyano, nitro, C₁-C₄-alkyl,         (phenyl)-(C₁-C₂-alkyl)-, C₁-C₆-haloalkyl, C₁-C₄-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₄-haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from hydroxy, cyano, nitro, (phenyl)-(C₁-C₂-alkyl)-,         C₁-C₄-haloalkyl, C₁-C₄-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-,         C₁-C₄haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         C₁-C₄-haloalkyl, C₁-C₄-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-,         C₁-C₄-haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted one or         two times, each substituent independently selected from a         halogen atom or a group selected from cyano, C₁-C₂-alkyl, and         C₁-C₂-alkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         C₁-C₄-haloalkyl, C₁-C₄-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-,         C₁-C₄-haloalkoxy, —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—OCH₂, —CH₂—O—, —O—CH₂—CH₂—O— and —O—CF₂O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl,         C₁-C₄-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₄-haloalkoxy,         —N(R⁵)(R⁶),         -   wherein the phenyl groups in said (phenyl)-(C₁-C₂-alkyl)-             and (phenyl)-(C₁-C₂-alkoxy)-groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from hydroxy, cyano,         C₁-C₄-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₂-fluoroalkoxy and —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted with one         methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from hydroxy, cyano,         C₁-C₃-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₂-fluoroalkoxy and —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from hydroxy, cyano,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-,         C₁-C₂-fluoroalkoxy and —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one, two,         or three times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from hydroxy, cyano, C₁-C₃-alkyl, C₁-C₂-fluoroalkyl,         C₁-C₂-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₂-fluoroalkoxy and         —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group selected             from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one, two,         or three times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from hydroxy, cyano, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         (phenyl)-(C₁-C₂-alkoxy)-, C₁-C₂-fluoroalkoxy and —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group selected             from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a pyridinyl group optionally substituted, one,         two, or three times, each substituent independently selected         from a fluorine atom, a chlorine atom and a bromine atom, or a         group selected from hydroxy, cyano, C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, (phenyl)-(C₁-C₂-alkoxy)-,         C₁-C₂-fluoroalkoxy and —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said pyridinyl group together form a bivalent group selected             from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from cyano, C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—, or     -   R¹ represents a pyrazolyl group optionally substituted with one         methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from cyano, C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a fluorine atom, a chlorine atom and         a bromine atom, or a group selected from cyano,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one, two,         or three times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from cyano, C₁-C₄-alkyl, C₁-C₂-fluoroalkyl,         C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one, two,         or three times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from cyano, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group selected             from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a pyridinyl group optionally substituted, one,         two, or three times, each substituent independently selected         from a fluorine atom, a chlorine atom and a bromine atom, or a         group selected from cyano, C₁-C₃-alkyl, C₁-C₂-fluoroalkyl,         C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R⁵)(R⁶),         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom and a chlorine atom, or a group selected from cyano,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom and a chlorine atom, or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom and a chlorine atom, or a group selected from cyano,         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom and a chlorine atom, or a group selected from         C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a pyridinyl group optionally substituted, one or         two times, each substituent independently selected from a         fluorine atom and a chlorine atom, or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from cyano, methyl, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from cyano, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom, a chlorine atom and a bromine atom, or a group selected         from cyano, methyl, difluoromethyl, trifluoromethyl, methoxy,         benzyloxy, difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom, a chlorine atom and a bromine atom, or a group selected         from cyano, difluoromethyl, trifluoromethyl, methoxy, benzyloxy,         difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a pyridinyl group optionally substituted, one or         two times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from cyano, methyl, difluoromethyl, trifluoromethyl,         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said pyridinyl group together form a bivalent group             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from difluoromethyl, trifluoromethyl,         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom, a chlorine atom and a bromine atom, or a group selected         from methyl, difluoromethyl, trifluoromethyl, methoxy,         benzyloxy, difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom, a chlorine atom and a bromine atom, or a group selected         from difluoromethyl, trifluoromethyl, methoxy, benzyloxy,         difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a pyridinyl group optionally substituted, one or         two times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from methyl, difluoromethyl, trifluoromethyl, methoxy,         benzyloxy, difluoromethoxy and trifluoromethoxy,         -   or two substituents attached to adjacent carbon atoms of             said pyridinyl group together form a bivalent group             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group wherein “**” indicates the point of         attachment to the nitrogen atom to which R¹ is attached;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═;     -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from difluoromethyl, trifluoromethyl,         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   Y¹ represents —C(H)═, —C(F)═ or —N═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   Y¹ represents —C(H)═, —C(F)═ or —N═;     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached;     -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═ or —N═;     -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from difluoromethyl, methoxy,         benzyloxy, difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached,     -   Y¹ represents —C(H)═ or —C(F)═, and     -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached, and     -   R²⁸ represents a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group selected from

-   -   wherein “**” indicates the point of attachment to the nitrogen         atom to which R¹ is attached,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹ represents a group selected from

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R¹ is attached,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

R² represents a group

and in a ratio of about 99:1 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

R² represents a group

in a ratio of about 98:2 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

R² represents a group

in a ratio of about 95:5 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

R² represents a group

in a ratio of about 90:10 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

R² represents a group

in a ratio of about 80:20 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

-   -   R² represents a group

in a ratio of about 50:50, that is, racemic mixtures in case of compounds featuring no further element of chirality,

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a C₁-C₂-alkyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula

-   -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R² represents a group

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

-   -   R² represents a group

in a ratio of about 99:1 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

R² represents a group

in a ratio of about 98:2 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

-   -   R² represents a group

in a ratio of about 95:5 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

-   -   R² represents a group

in a ratio of about 90:10 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

-   -   R² represents a group

in a ratio of about 80:20 and higher,

-   -   in favour of

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers isomeric mixtures of compounds of formula (I), supra, in which:

-   -   R² represents a group

in a ratio of about 50:50, that is, racemic mixtures in case of compounds featuring no further element of chirality,

-   -   wherein “*” indicates the point of attachment to the nitrogen         atom to which R² is attached;     -   R⁷ represents a methyl group;     -   R⁸ represents a —C(═O)—NH₂ group,     -   and tautomers, N-oxides, hydrates, solvates, and salts thereof,         and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R³ represents a group selected from methyl and —NH₂,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R³ represents a —NH₂ group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R³ represents a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         (5-membered heteroaryl)-(C₁-C₂-alkyl)-,         (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)-, ((R⁹)O)—(C₁-C₄-alkyl)-,         C₁-C₄-haloalkyl, C₃-C₇-cycloalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         ((R¹⁰)(R¹¹)N)—(C₁-C₃-alkyl)-, —C(═O)—N(R¹²)(R¹³), S(═O), R¹⁴,         —C(═O)R¹⁴, —C(═O)—OR¹⁷, and a 5-membered heteroaryl group which         itself is optionally substituted with one or two methyl groups,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, nitro, C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-,         (5-membered heteroaryl)-(C₁-C₂-alkyl)-C₁-C₄-hydroxyalkyl,         C₁-C₄-haloalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³), S(═O),         —R¹⁴, and a 5-membered heteroaryl group which itself is         optionally substituted with one or two methyl groups,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —(CH₂)₄—, —(CH₂)₂—O—,             —(CH₂)₃—O—, —CH₂—O—CH₂—, —O—CH₂—O—, —O—CH₂—CH₂—O— and             —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one, two, or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, C₁-C₃-alkyl, ((R⁹)O)—(C₁-C₃-alkyl)-,         C₁-C₃-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³),         S(═O)_(n)—R¹⁴ and —C(═O)—OR¹⁷,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl group optionally substituted, one, two,         or three times, each substituent independently selected from a         halogen atom or a group selected from cyano, C₁-C₃-alkyl,         ((R⁹)O)—(C₁-C₃-alkyl)-, C₁-C₃-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³), S(═O)_(n)—R¹⁴ and —C(═O)—OR¹⁷,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a pyridinyl group optionally substituted, one,         two, or three times, each substituent independently selected         from a halogen atom or a group selected from cyano, C₁-C₃-alkyl,         ((R⁹)O)—(C₁-C₃-alkyl)-, C₁-C₃-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³), S(═O)_(n)—R¹⁴ and —C(═O)—OR¹⁷,         -   or two substituents attached to adjacent carbon atoms of             said phenyl or pyridinyl group together form a bivalent             group selected from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl group optionally substituted, one, two,         or three times, each substituent independently selected from a         fluorine atom, a chlorine atom and a bromine atom, or a group         selected from cyano, C₁-C₃-alkyl, CrC₃-hydroxyalkyl         C₁-C₃-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³) and         S(═O)_(n)—R¹⁴         -   or two substituents attached to adjacent carbon atoms of             said phenyl group together form a bivalent group selected             from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a pyridinyl group optionally substituted, one,         two, or three times, each substituent independently selected         from a fluorine atom, a chlorine atom and a bromine atom, or a         group selected from cyano, C₁-C₃-alkyl, C₁-C₃-hydroxyalkyl,         C₁-C₃-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³) and         S(═O)_(n)—R¹⁴,         -   or two substituents attached to adjacent carbon atoms of             said pyridinyl group together form a bivalent group selected             from —(CH₂)₃—, —O—CH₂—O— and —O—CF₂—O—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹;     -   R⁹ represents a group selected from C₁-C₂-alkyl, benzyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkoxy)-C₂-alkyl- and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a halogen atom or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and —C(═O)—OR¹⁷;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a halogen         atom or a group selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl,         —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³) and —C(═O)—OR¹⁷;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a pyridinyl group optionally substituted, one or         two times, each substituent independently selected from a         halogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹), —C(═O)—N(R¹²)(R¹³) and         —C(═O)—OR¹⁷;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl or pyridinyl group optionally         substituted, one or two times, each substituent independently         selected from a fluorine atom and a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom and a chlorine atom, or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, —OR⁹;     -   R⁹ represents a group selected from C₁-C₂-alkyl, benzyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkoxy)-C₂-alkyl- and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a phenyl group optionally substituted, one or two         times, each substituent independently selected from a fluorine         atom and a chlorine atom, or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, —OR⁹,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a pyridinyl group optionally substituted, one or         two times, each substituent independently selected from a         fluorine atom and a chlorine atom, or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹;     -   R⁹ represents a group selected from C₁-C₂-alkyl, benzyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkoxy)-C₂-alkyl- and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a pyridinyl group optionally substituted, one or         two times, each substituent independently selected from a         fluorine atom and a chlorine atom, or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁷ represents a halogen atom or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and —C(═O)OR¹⁷,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   Y² represents —C(H)═ or —N═;     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents —N═, Y² represents —C(H)═;     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   Y² represents —C(H)═ and —N═;     -   Y³ represents —C(R²⁷)═ and —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y³ represents N═, Y² represents —C(H)═;     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached;     -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group selected from:

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁴ represents a group selected from:

-   -   wherein “#” indicates the point of attachment to the carbonyl         group to which R⁴ is attached,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from oxo, hydroxy, C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom, a chlorine atom and a bromine         atom, or a group selected from oxo, hydroxy, C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group, or     -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from hydroxy         and C₁-C₂-alkyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁵ and R⁶ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁵ and R⁶, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from hydroxy         and C₁-C₂-alkyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁷ represents a hydrogen atom,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁷ represents a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁷ represents an ethyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁷ represents a hydrogen atom or a C₁-C₂-alkyl group;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl,         C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-, —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹), —C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-group and said phenyl group itself             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a bromine atom, or a group selected from             cyano, methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₄-alkyl, (phenyl)-(C₁-C₂-alkyl)-, C₁-C₄-haloalkyl,         C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-, —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-group and said phenyl group itself             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a bromine atom, or a group selected from             methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl, C₂-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷, —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹),         —C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—OR¹⁷, —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹) and         phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a group selected from C₁-C₂-alkyl, benzyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkoxy)-C₂-alkyl- and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom, a chlorine atom and a methyl group;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl,         (C₁-C₂-alkoxy)-C₂-alkyl-, ((C₁-C₂-alkyl)-C(═O)—O)—C₂-alkyl-,         —C(R¹⁸)(R¹⁹)—C(═O)—N(R²⁰)(R²¹), —C(═O)—N(R²⁰)(R²¹) and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R⁹ represents a hydrogen atom or a group selected from         C₁-C₂-alkyl, benzyl, C₁-C₂-fluoroalkyl and phenyl,         -   wherein the phenyl group within said benzyl group and said             phenyl group itself are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             cyano and methyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl, C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((R²²)(R²³)N)—C₂-alkyl, (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₇-cycloalkyl,         (C₃-C₇-cycloalkyl)-C(═O)—, (phenyl)-(C₁-C₂-alkyl)-,         (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₇cycloalkyl within             said (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)- and             (C₃-C₇-cycloalkyl)-C(═O)— groups are optionally substituted             one or two times, each substituent independently selected             from a fluorine atom or a group selected from cyano,             C₁-C₂-alkyl and C₁-C₂-haloalkyl,         -   and wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 5- to 10-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₂-alkyl, C₁-C₂-haloalkyl,         (C₁-C₂-alkyl)-C(═O)—, C₁-C₂-alkoxy, —N(R²²)(R²³), and a         monocyclic 4- to 7-membered heterocycloalkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl, C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((R²²)(R²³)N)—C₂-alkyl, (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₇-cycloalkyl,         (C₃-C₇-cycloalkyl)-C(═O)—, (phenyl)-(C₁-C₂-alkyl)-,         (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₇cycloalkyl within             said (C₃-C₇-cycloalkyl)-(C₁-C₂-alkyl)- and             (C₃-C₇-cycloalkyl)-C(═O)— groups are optionally substituted             one or two times, each substituent independently selected             from a fluorine atom or a group selected from cyano,             C₁-C₂-alkyl and C₁-C₂-haloalkyl,         -   and wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 5- to 10-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₂-alkyl, C₁-C₂-haloalkyl,         (C₁-C₂-alkyl)-C(═O)—, C₁-C₂-alkoxy, —N(R²²)(R²³), and a         monocyclic 4- to 7-membered heterocycloalkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl, C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((R²²)(R²³)N)—C₂-alkyl, (C₁-C₂-alkyl)-C(═O)—, C₃-C₅-cycloalkyl,         (C₃-C₅-cycloalkyl)-C(═O)—, (phenyl)-(C₁-C₂-alkyl)-,         (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 6- to 10-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₂-alkyl, C₁-C₂-haloalkyl,         (C₁-C₂-alkyl)-C(═O)—, C₁-C₂-alkoxy, —N(R²²)(R²³), and a         monocyclic 4- to 7-membered heterocycloalkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl, C₂-C₃-hydroxyalkyl, (C₁-C₂-alkoxy)-C₂-alkyl-,         ((R²²)(R²³)N)—C₂-alkyl, (C₁-C₂-alkyl)-C(═O)—, C₃-C₅-cycloalkyl,         (C₃-C₅-cycloalkyl)-C(═O)—, (phenyl)-(C₁-C₂-alkyl)-,         (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a bromine             atom, or a group selected from methyl, trifluoromethyl and             methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group, or a bicyclic nitrogen         containing 6- to 10-membered heterocycloalkyl group, which are         optionally substituted one, two or three times, each substituent         independently selected from a halogen atom or a group selected         from cyano, oxo, hydroxy, C₁-C₂-alkyl, C₁-C₂-haloalkyl,         (C₁-C₂-alkyl)-C(═O)—, C₁-C₂-alkoxy, —N(R²²)(R²³), and a         monocyclic 4- to 7-membered heterocycloalkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-(C₁-C₂)-alkyl)-C(═O)—,         C₃-C₇-cycloalkyl, C₃-C₇-cycloalkyl-(C═O)—,         (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)- and the             C₃-C₇-cycloalkyl within the C₃-C₇-cycloalkyl-(C═O)— groups             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom or a             group selected from cyano, C₁-C₂-alkyl and             C₁-C₂-fluoroalkyl,         -   and wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         oxo, C₁-C₂-alkyl, C₁-C₂-fluoroalkyl and (C₁-C₂)-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-(C₁-C₂)-alkyl)-C(═O)—,         C₃-C₇-cycloalkyl, C₃-C₇-cycloalkyl-(C═O)—,         (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)- and the             C₃-C₇-cycloalkyl within the C₃-C₇-cycloalkyl-(C═O)— groups             are optionally substituted one or two times, each             substituent independently selected from a fluorine atom or a             group selected from cyano, C₁-C₂-alkyl and             C₁-C₂-fluoroalkyl,         -   and wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂)-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         oxo, C₁-C₂-alkyl, C₁-C₂-fluoroalkyl and (C₁-C₂)-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkyl)-C(═O)—,         (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from oxo,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₁-C₂-fluoroalkyl, (C₁-C₂-alkyl)-C(═O)—,         (phenyl)-(C₁-C₂-alkyl)-(phenyl)-(C₁-C₂-alkyl)-C(═O)— and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein the phenyl groups within said             (phenyl)-(C₁-C₂-alkyl)-, (phenyl)-(C₁-C₂-alkyl)-C(═O)— and             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— groups are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from oxo,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-group are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom or a group selected from             cyano, methyl and C₁-fluoroalkyl,         -   and wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— group is optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and C₁-fluoroalkyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl, and the C₃-C₅-cycloalkyl within             said (C₃-C₅-cycloalkyl)-(C₁-C₂-alkyl)-group are optionally             substituted one or two times, each substituent independently             selected from a fluorine atom or a group selected from             cyano, methyl and C-fluoroalkyl,         -   and wherein the phenyl group within said             (phenyl)-(C₁-C₂-alkyl)-O—C(═O)— group is optionally             substituted one or two times, each substituent independently             selected from a fluorine atom, a chlorine atom and a methyl             group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and C₁-fluoroalkyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₃-C₇-cycloalkyl and (benzyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom or a group selected from methyl and             trifluoromethyl,         -   and wherein the phenyl group within said (benzyl)-O—C(═O)—             group is optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a methyl group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and trifluoromethyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         C₃-C₇-cycloalkyl and (benzyl)-O—C(═O)—,         -   wherein C₃-C₇-cycloalkyl is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom or a group selected from methyl and             trifluoromethyl,         -   and wherein the phenyl group within said (benzyl)-O—C(═O)—             group is optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and trifluoromethyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (benzyl)-O—C(═O)—,         -   and wherein the phenyl group within said (benzyl)-O—C(═O)—             group is optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a methyl group, or     -   R¹⁰ and R¹¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a fluorine atom or a group selected from cyano,         methyl and trifluoromethyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁰ and R¹¹ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (benzyl)-O—C(═O)—,         -   and wherein the phenyl group within said (benzyl)-O—C(═O)—             group is optionally substituted one or two times, each             substituent independently selected from a fluorine atom, a             chlorine atom and a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₁-C₆-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₄-haloalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             halogen atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₃-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from cyano, methyl, trifluoromethyl and             methoxy, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₄-haloalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, 3-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             halogen atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₃-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from cyano, methyl, trifluoromethyl and             methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₁-C₆-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             halogen atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)- or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-haloalkyl, C₁-C₃-hydroxyalkyl,         (C₁-C₂-alkoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₃-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             halogen atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-fluoroalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-fluoroalkyl, C₁-C₄-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl, monocyclic 4- to         7-membered heterocycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-fluoroalkyl, C₃-C₇-cycloalkyl, monocyclic 4- to 7-membered         heterocycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered             heterocycloalkyl are optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a group selected from oxo, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—, or     -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₄-fluoroalkyl, C₃-C₇-cycloalkyl, monocyclic 4- to 7-membered         heterocycloalkyl and (phenyl)-(C₁-C₂-alkyl)-, wherein         C₃-C₇-cycloalkyl and monocyclic 4- to 7-membered         heterocycloalkyl are optionally substituted one or two times,         each substituent independently selected from a fluorine atom or         a group selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         substituted one or two times, each substituent independently         selected from a halogen atom or a group selected from oxo,         C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-hydroxyalkyl,         (C₁-C₄-alkoxy)-C₂-C₃-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-C₃-alkyl-,         (phenoxy)-C₂-C₃-alkyl-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-,         -   wherein C₃-C₇cycloalkyl is optionally substituted one or two             times, each substituent independently selected from a             fluorine atom or a methyl group,         -   and wherein the phenyl groups within said             (phenoxy)-C₂-C₃-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from a             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₄-alkyl,         C₁-C₂-fluoroalkyl, C₁-C₂-hydroxyalkyl, (C₁-C₄-alkoxy)-C₂-alkyl-,         (C₁-C₂-fluoroalkoxy)-C₂-alkyl-, (phenoxy)-C₂-alkyl-,         C₃-C₇-cycloalkyl and (phenyl)-(C₁-C₂-alkyl)-,         -   wherein the phenyl groups within said             (phenoxy)-C₂-alkyl-group and said             (phenyl)-(C₁-C₂-alkyl)-group are optionally substituted one             or two times, each substituent independently selected from             fluorine atom and a chlorine atom, or a group selected from             methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹² and R¹³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl,         (C₁-C₄-alkoxy)-C₂-alkyl-, (C₁-C₂-fluoroalkoxy)-C₂-alkyl-,         (phenoxy)-C₂-alkyl-, C₃-C₇-cycloalkyl and         (phenyl)-(C₁-C₂-alkyl)-,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁴ represents a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl and phenyl,         -   wherein the phenyl group is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁴ represents a group selected from C₁-C₂-alkyl,         C₁-C₂-haloalkyl and phenyl,         -   wherein the phenyl group is optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁴ represents a group selected from C₁-C₂-alkyl and         C₁-C₂-haloalkyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁴ represents a group selected from methyl and trifluoromethyl,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁴ represents a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁷ represents a C₁-C₄-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁷ represents a C₁-C₃-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁷ represents a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁷ represents a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁷ represents an ethyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁸ and R¹⁹ represent, independently from each occurrence, a         hydrogen atom or a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁸ and R¹⁹ both represent a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁸ and R¹⁹ both represent a hydrogen atom,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R¹⁸ represents a hydrogen atom and R¹⁹ represents a methyl         group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,             monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to             10-membered heterocycloalkyl, phenyl, and 5- to 10-membered             heteroaryl, said phenyl and 5- to 10-membered heteroaryl             substituents themselves being optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,         -   wherein C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 6- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₅-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³), C₃-C₇cycloalkyl,             bicyclic C₆-C₁₁-cycloalkyl, adamantyl, monocyclic 4- to             7-membered heterocycloalkyl, bicyclic 6- to 10-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a bromine atom, or a group             selected from methyl, trifluoromethyl and methoxy,         -   wherein C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 6- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵)     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 5- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³), C₃-C₇cycloalkyl,             bicyclic C₅-C₁₁-cycloalkyl, adamantyl, monocyclic 4- to             7-membered heterocycloalkyl, bicyclic 5- to 10-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a bromine atom, or a group             selected from methyl, trifluoromethyl and methoxy,         -   and wherein C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 5- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵), and     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁—OC-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 5- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³), 3-C₇-cycloalkyl,             bicyclic C₅-C₁₁-cycloalkyl, adamantyl, monocyclic 4- to             7-membered heterocycloalkyl, bicyclic 5- to 10-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a bromine atom, or a group             selected from methyl, trifluoromethyl and methoxy,         -   and wherein C₃-C₇-cycloalkyl, bicyclic C₅-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 5- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵), and     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,             monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to             10-membered heterocycloalkyl, phenyl, and 5- to 10-membered             heteroaryl, said phenyl and 5- to 10-membered heteroaryl             substituents themselves being optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,         -   wherein C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 6- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₆-alkyl, C₃-C₄-alkenyl, C₃-C₄-alkynyl, C₁-C₃-alkoxy,         C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl, adamantyl,         monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to         10-membered heterocycloalkyl, phenyl, naphthyl, and 5- to         10-membered heteroaryl,         -   wherein said C₁-C₆-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₇-cycloalkyl, bicyclic C₆-C₁₁ cycloalkyl, adamantyl,             monocyclic 4- to 7-membered heterocycloalkyl, bicyclic 6- to             10-membered heterocycloalkyl, phenyl, and 5- to 10-membered             heteroaryl, said phenyl and 5- to 10-membered heteroaryl             substituents themselves being optionally substituted one or             two times, each substituent independently selected from a             fluorine atom, a chlorine atom and a bromine atom, or a             group selected from methyl, trifluoromethyl and methoxy,         -   wherein C₃-C₇cycloalkyl, bicyclic C₆-C₁₁-cycloalkyl,             adamantyl, monocyclic 4- to 7-membered heterocycloalkyl,             bicyclic 6- to 10-membered heterocycloalkyl are optionally             substituted one or two or three times, each substituent             independently selected from a halogen atom or a group             selected from cyano, oxo, hydroxy, C₁-C₂-alkyl and             (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl, naphthyl and 5- to 10-membered             heteroaryl groups are optionally substituted one, two or             three times, each substituent independently selected from a             halogen atom or a group selected from cyano, C₁-C₂-alkyl,             C₁-C₂-haloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy,             —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-haloalkyl, (phenyl)-(C₁-C₂-alkyl)-,         (C₁-C₂-alkyl)-C(═O)—, C₃-C₄-cycloalkyl, C₁-C₂-alkoxy,         C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵);     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic 4-             to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic 4-             to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵);     -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—;     -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic 4-             to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵),     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic 4-             to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-haloalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-haloalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵);     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁_Cu-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   and wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic             4- to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-fluoroalkoxy, —N(R²²)(R²³) and —C(═O)—N(R²⁴)(R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group, or     -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵),     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         optionally substituted C₁-C₃-alkyl, unsubstituted C₄-C₆-alkyl,         prop-2-ynyl, methoxy, C₃-C₆-cycloalkyl, adamantyl, monocyclic 4-         to 7-membered heterocycloalkyl, phenyl, and 5- to 10-membered         heteroaryl,         -   wherein said C₁-C₃-alkyl group is optionally substituted             one, two or three times, each substituent independently             selected from a halogen atom or a group selected from             hydroxy, cyano, C₁-C₃-alkoxy, —N(R²²)(R²³),             C₃-C₆-cycloalkyl, adamantyl, monocyclic 4- to 7-membered             heterocycloalkyl, phenyl, and 5- to 10-membered heteroaryl,             said phenyl and 5- to 10-membered heteroaryl substituents             themselves being optionally substituted one or two times,             each substituent independently selected from a fluorine             atom, a chlorine atom and a methyl group,         -   and wherein said C₃-C₆-cycloalkyl, adamantyl and monocyclic             4- to 7-membered heterocycloalkyl groups are optionally             substituted one or two or three times, each substituent             independently selected from a fluorine atom or a group             selected from oxo, C₁-C₂-alkyl and (C₁-C₂-alkyl)-C(═O)—,         -   and wherein said phenyl and 5- to 10-membered heteroaryl             groups are optionally substituted one, two or three times,             each substituent independently selected from a fluorine atom             and a chlorine atom or a group selected from cyano,             C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy,             C₁-C₂-fluoroalkoxy, —N(R²²)(R²³) and —C(═O)—N (R²⁴) (R²⁵),     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ and R²¹, together with the nitrogen atom to which they are         attached, represent a monocyclic nitrogen containing 4- to         7-membered heterocycloalkyl group which is optionally         benzocondensed, and which is optionally substituted one, two or         three times, each substituent independently selected from a         halogen atom or a group selected from cyano, oxo, hydroxy,         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, benzyl, (C₁-C₂-alkyl)-C(═O)—,         C₃-C₄-cycloalkyl, C₁-C₂-alkoxy, C₁-C₂-fluoroalkoxy, —N(R²²)(R²³)         and —C(═O)—N(R²⁴)(R²⁵),     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a hydrogen atom or a group selected from         C₁-C₃-alkyl and phenyl,         -   wherein said C₁-C₃-alkyl group is optionally substituted one             or two times, each substituent independently selected from a             fluorine atom or a group selected from hydroxy, C₁-C₃-alkoxy             and phenyl, said phenyl itself being optionally substituted             one or two times, each substituent independently selected             from a fluorine atom, a chlorine atom and a methyl group,         -   and wherein said phenyl group is optionally substituted one,             two or three times, each substituent independently selected             from a fluorine atom and a chlorine atom or a group selected             from methyl, trifluoromethyl, methoxy and trifluoromethoxy;     -   R²¹ represents a hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁰ represents a group selected from benzyl and phenyl, wherein         said phenyl group, and the phenyl group within said benzyl         group, is optionally substituted one or two times, each         substituent independently selected from a fluorine atom, a         chlorine atom and a methyl group,     -   R²¹ represents a hydrogen atom or a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from C₁-C₂-alkyl and         (C₁-C₂-alkyl)-C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²² and R²³ represent, independently from each occurrence, a         hydrogen atom or a group selected from methyl and (CH₃)—C(═O)—,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a C₁-C₂-alkyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁴ and R²⁵ represent, independently from each occurrence, a         hydrogen atom or a methyl group,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, C₁-C₂-alkoxy and         C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from methyl, difluoromethyl,         trifluoromethyl, methoxy, benzyloxy, difluoromethoxy and         trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from methyl, difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁶ represents a fluorine atom, a chlorine atom or a bromine         atom, or a group selected from difluoromethyl, methoxy,         benzyloxy, difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁶ represents a fluorine atom, a chlorine atom, or a group         selected from difluoromethyl, trifluoromethyl, methoxy,         difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁷ represents a halogen atom or a group selected from         C₁-C₂-alkyl, C₁-C₂-fluoroalkyl, —OR⁹, —N(R¹⁰)(R¹¹),         —C(═O)—N(R¹²)(R¹³) and —C(═O)OR¹⁷,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁷ represents a fluorine atom, a chlorine atom, or a group         selected from C₁-C₂-alkoxy, benzyloxy and C₁-C₂-fluoroalkoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁷ represents a halogen atom or a group selected from —OR⁹,         —N(R¹⁰)(R¹¹) and —C(═O)—N(R¹²)(R¹³),     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   R²⁷ represents a chlorine atom, or a group selected from         methoxy, benzyloxy, difluoromethoxy and trifluoromethoxy,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   n represents an integer 0, 1, or 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   n represents an integer 0 or 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   n represents an integer 0,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   n represents an integer 2,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═, —C(CN)═ or —N═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —C(H)═, —C(F)═, —C(Cl)═ or —N═;     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —C(H)═, —C(F)═ or —N═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —C(H)═ or —C(F)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —C(H)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —C(Cl)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y represents —C(F)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y¹ represents —N═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y² represents —C(H)═ or —N═,     -   Y³ represents —C(R²⁷)═ or —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y¹ represents —N═, Y² represents —C(H)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y² represents —C(H)═ and —N═,     -   Y³ represents —C(R²⁷)═ and —N═,         -   with the proviso that if Y² represents —N═, Y³ represents             —C(R²⁷)═, and if Y¹ represents —N═, Y² represents —C(H)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y² represents —N═;     -   Y³ represents —C(R²⁷)     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y² represents —C(H)═;     -   Y³ represents —N═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a further embodiment of the first aspect, the present invention covers compounds of formula (I), supra, in which:

-   -   Y² represents —C(H)═;     -   Y³ represents —C(R²⁷)═,     -   and stereoisomers, tautomers, N-oxides, hydrates, solvates, and         salts thereof, and mixtures of same.

In a particular further embodiment of the first aspect, the present invention covers combinations of two or more of the above mentioned embodiments under the heading “further embodiments of the first aspect of the present invention”.

The present invention covers any sub-combination within any embodiment or aspect of the present invention of compounds of general formula (I), supra.

The present invention covers the compounds of general formula (I) which are disclosed in the Example Section of this text, infra.

The compounds of general formula (I) of the present invention can be converted to any salt, preferably pharmaceutically acceptable salts, as described herein, by any method which is known to the person skilled in the art. Similarly, any salt of a compound of general formula (I) of the present invention can be converted into the free compound, by any method which is known to the person skilled in the art.

Compounds of general formula (I) of the present invention demonstrate a valuable pharmacological spectrum of action, which could not have been predicted. Compounds of the present invention have surprisingly been found to effectively inhibit DGKζ and it is possible therefore that said compounds be used for the treatment or prophylaxis of diseases, preferably conditions with dysregulated immune responses, particularly cancer or other disorders associated with aberrant DGKζ signaling, in mammals, including humans.

Disorders and conditions particularly suitable for treatment with an DGKζ inhibitor of the present invention are liquid and solid tumours, such as cancers of the breast, respiratory tract, brain, reproductive organs, digestive tract, urinary tract, eye, liver, skin, head and neck, thyroid, parathyroid and their distant metastases. Those disorders also include lymphomas, sarcomas, and leukaemias.

Examples of breast cancers include, but are not limited to, triple negative breast cancer, invasive ductal carcinoma, invasive lobular carcinoma, ductal carcinoma in situ, and lobular carcinoma in situ.

Examples of cancers of the respiratory tract include, but are not limited to, small-cell and non-small-cell lung carcinoma, as well as bronchial adenoma and pleuropulmonary blastoma.

Examples of brain cancers include, but are not limited to, brain stem and hypothalmic glioma, cerebellar and cerebral astrocytoma, glioblastoma, medulloblastoma, ependymoma, as well as neuroectodermal and pineal tumour.

Tumours of the male reproductive organs include, but are not limited to, prostate and testicular cancer.

Tumours of the female reproductive organs include, but are not limited to, endometrial, cervical, ovarian, vaginal, and vulvar cancer, as well as sarcoma of the uterus.

Examples of ovarian cancer include, but are not limited to serous tumour, endometrioid tumour, mucinous cystadenocarcinoma, granulosa cell tumour, Sertoli-Leydig cell tumour and arrhenoblastoma.

Examples of cervical cancer include, but are not limited to squamous cell carcinoma, adenocarcinoma, adenosquamous carcinoma, small cell carcinoma, neuroendocrine tumour, glassy cell carcinoma and villoglandular adenocarcinoma.

Tumours of the digestive tract include, but are not limited to, anal, colon, colorectal, esophageal, gallbladder, gastric, pancreatic, rectal, small-intestine, and salivary gland cancers.

Examples of esophageal cancer include, but are not limited to esophageal cell carcinomas and adenocarcinomas, as well as squamous cell carcinomas, leiomyosarcoma, malignant melanoma, rhabdomyosarcoma and lymphoma.

Examples of gastric cancer include, but are not limited to intestinal type and diffuse type gastric adenocarcinoma.

Examples of pancreatic cancer include, but are not limited to ductal adenocarcinoma, adenosquamous carcinomas and pancreatic endocrine tumours.

Tumours of the urinary tract include, but are not limited to, bladder, penile, kidney, renal pelvis, ureter, urethral and human papillary renal cancers.

Examples of kidney cancer include, but are not limited to renal cell carcinoma, urothelial cell carcinoma, juxtaglomerular cell tumour (reninoma), angiomyolipoma, renal oncocytoma, Bellini duct carcinoma, clear-cell sarcoma of the kidney, mesoblastic nephroma and Wilms' tumour.

Examples of bladder cancer include, but are not limited to transitional cell carcinoma, squamous cell carcinoma, adenocarcinoma, sarcoma and small cell carcinoma.

Eye cancers include, but are not limited to, intraocular melanoma and retinoblastoma.

Examples of liver cancers include, but are not limited to, hepatocellular carcinoma (liver cell carcinomas with or without fibrolamellar variant), cholangiocarcinoma (intrahepatic bile duct carcinoma), and mixed hepatocellular cholangiocarcinoma.

Skin cancers include, but are not limited to, squamous cell carcinoma, Kaposi's sarcoma, malignant melanoma, Merkel cell skin cancer, and non-melanoma skin cancer.

Head-and-neck cancers include, but are not limited to, squamous cell cancer of the head and neck, laryngeal, hypopharyngeal, nasopharyngeal, oropharyngeal cancer, salivary gland cancer, lip and oral cavity cancer and squamous cell.

Lymphomas include, but are not limited to, AIDS-related lymphoma, non-Hodgkin's lymphoma, cutaneous T-cell lymphoma, Burkitt lymphoma, Hodgkin's disease, and lymphoma of the central nervous system.

Sarcomas include, but are not limited to, sarcoma of the soft tissue, osteosarcoma, malignant fibrous histiocytoma, lymphosarcoma, and rhabdomyosarcoma.

Leukemias include, but are not limited to, acute myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia, chronic myelogenous leukemia, and hairy cell leukemia.

The term “treating” or “treatment” as stated throughout this document is used conventionally, for example the management or care of a subject for the purpose of combating, alleviating, reducing, relieving, improving the condition of a disease or disorder, such as a carcinoma.

The compounds of the present invention can be used in particular in therapy and prevention, i.e. prophylaxis, of tumour growth and metastases, especially in solid tumours of all indications and stages with or without pre-treatment of the tumour growth.

Generally, the use of chemotherapeutic agents and/or anti-cancer agents in combination with a compound or pharmaceutical composition of the present invention will serve to:

-   -   1. yield better efficacy in reducing the growth of a tumour or         even eliminate the tumour as compared to administration of         either agent alone,     -   2. provide for the administration of lesser amounts of the         administered chemotherapeutic agents,     -   3. provide for a chemotherapeutic treatment that is well         tolerated in the patient with fewer deleterious pharmacological         complications than observed with single agent chemotherapies and         certain other combined therapies,     -   4. provide for treating a broader spectrum of different cancer         types in mammals, especially humans,     -   5. provide for a higher response rate among treated patients,     -   6. provide for a longer survival time among treated patients         compared to standard chemotherapy treatments,     -   7. provide a longer time for tumour progression, and/or     -   8. yield efficacy and tolerability results at least as good as         those of the agents used alone, compared to known instances         where other cancer agent combinations produce antagonistic         effects.

In addition, the compounds of general formula (I) of the present invention can also be used in combination with radiotherapy and/or surgical intervention.

In a further embodiment of the present invention, the compounds of general formula (I) of the present invention are used in combination with radiation: i.e. radiation treatment sensitizes cancers to anti-tumor immune responses by induction of tumor cell death and subsequent presentation of tumor neoantigens to tumor-reactive Tcells. As DGKζ is enhancing the antigen specific activation of T cells, the overall effect results in a much stronger cancer cell attack as compared to irradiation treatment alone.

Thus, the present invention also provides a method of killing a tumor, wherein conventional radiation therapy is employed previous to administering one or more of the compounds of the present invention.

The compounds of the present invention can be administered as the sole pharmaceutical agent or in combination with one or more other pharmaceutically active ingredients where the combination causes no unacceptable adverse effects. The present invention also covers such pharmaceutical combinations. For example, the compounds of the present invention can be combined with:

131I-chTNT, abarelix, abemaciclib, abiraterone, acalabrutinib, aclarubicin, adalimumab, ado-trastuzumab emtansine, afatinib, aflibercept, aldesleukin, alectinib, alemtuzumab, alendronic acid, alitretinoin, alpharadin, altretamine, amifostine, aminoglutethimide, hexyl aminolevulinate, amrubicin, amsacrine, anastrozole, ancestim, anethole dithiolethione, anetumab ravtansine, angiotensin II, antithrombin III, apalutamide, aprepitant, arcitumomab, arglabin, arsenic trioxide, asparaginase, atezolizumab, avelumab, axicabtagene ciloleucel, axitinib, azacitidine, basiliximab, belotecan, bendamustine, besilesomab, belinostat, bevacizumab, bexarotene, bicalutamide, bisantrene, bleomycin, blinatumomab, bortezomib, bosutinib, buserelin, brentuximab vedotin, brigatinib, busulfan, cabazitaxel, cabozantinib, calcitonine, calcium folinate, calcium levofolinate, capecitabine, capromab, carbamazepine carboplatin, carboquone, carfilzomib, carmofur, carmustine, catumaxomab, celecoxib, celmoleukin, cemiplimab, ceritinib, cetuximab, chlorambucil, chlormadinone, chlormethine, cidofovir, cinacalcet, cisplatin, cladribine, clodronic acid, clofarabine, cobimetinib, copanlisib, crisantaspase, crizotinib, cyclophosphamide, cyproterone, cytarabine, dacarbazine, dactinomycin, daratumumab, darbepoetin alfa, dabrafenib, dasatinib, daunorubicin, decitabine, degarelix, denileukin diftitox, denosumab, depreotide, deslorelin, dianhydrogalactitol, dexrazoxane, dibrospidium chloride, dianhydrogalactitol, diclofenac, dinutuximab, docetaxel, dolasetron, doxifluridine, doxorubicin, doxorubicin+estrone, dronabinol, durvalumab, eculizumab, edrecolomab, elimusertib (BAY1895344), elliptinium acetate, elotuzumab, eltrombopag, enasidenib, endostatin, enocitabine, enzalutamide, epirubicin, epitiostanol, epoetin alfa, epoetin beta, epoetin zeta, eptaplatin, eribulin, erlotinib, esomeprazole, estradiol, estramustine, ethinylestradiol, etoposide, everolimus, exemestane, fadrozole, fentanyl, filgrastim, fluoxymesterone, floxuridine, fludarabine, fluorouracil, flutamide, folinic acid, formestane, fosaprepitant, fotemustine, fulvestrant, gadobutrol, gadoteridol, gadoteric acid meglumine, gadoversetamide, gadoxetic acid, gallium nitrate, ganirelix, gefitinib, gemcitabine, gemtuzumab, Glucarpidase, glutoxim, GM-CSF, goserelin, granisetron, granulocyte colony stimulating factor, histamine dihydrochloride, histrelin, hydroxycarbamide, I-125 seeds, lansoprazole, ibandronic acid, ibritumomab tiuxetan, ibrutinib, idarubicin, ifosfamide, imatinib, imiquimod, improsulfan, indisetron, incadronic acid, ingenol mebutate, inotuzumab ozogamicin, interferon alfa, interferon beta, interferon gamma, iobitridol, iobenguane (123I), iomeprol, ipilimumab, irinotecan, Itraconazole, ixabepilone, ixazomib, lanreotide, lansoprazole, lapatinib, lasocholine, lenalidomide, lenvatinib, lenograstim, lentinan, letrozole, leuprorelin, levamisole, levonorgestrel, levothyroxine sodium, lisuride, lobaplatin, lomustine, lonidamine, lutetium Lu 177 dotatate, masoprocol, medroxyprogesterone, megestrol, melarsoprol, melphalan, mepitiostane, mercaptopurine, mesna, methadone, methotrexate, methoxsalen, methylaminolevulinate, methylprednisolone, methyltestosterone, metirosine, midostaurin, mifamurtide, miltefosine, miriplatin, mitobronitol, mitoguazone, mitolactol, mitomycin, mitotane, mitoxantrone, mogamLlizumab, molgramostim, mopidamol, morphine hydrochloride, morphine sulfate, mvasi, nabilone, nabiximols, nafarelin, naloxone+pentazocine, naltrexone, nartograstim, necitumumab, nedaplatin, nelarabine, neratinib, neridronic acid, netupitant/palonosetron, nivolumab, pentetreotide, nilotinib, nilutamide, nimorazole, nimotuzumab, nimustine, nintedanib, niraparib, nitracrine, nivolumab, obinutuzumab, octreotide, ofatumumab, olaparib, olaratumab, omacetaxine mepesuccinate, omeprazole, ondansetron, oprelvekin, orgotein, orilotimod, osimertinib, oxaliplatin, oxycodone, oxymetholone, ozogamicine, p53 gene therapy, paclitaxel, palbociclib, palifermin, palladium-103 seed, palonosetron, pamidronic acid, panitumumab, panobinostat, pantoprazole, pazopanib, pegaspargase, PEG-epoetin beta (methoxy PEG-epoetin beta), pembrolizumab, pegfilgrastim, peginterferon alfa-2b, pemetrexed, pentazocine, pentostatin, peplomycin, Perflubutane, perfosfamide, Pertuzumab, picibanil, pilocarpine, pirarubicin, pixantrone, plerixafor, plicamycin, poliglusam, polyestradiol phosphate, polyvinylpyrrolidone+sodium hyaluronate, polysaccharide-K, pomalidomide, ponatinib, porfimer sodium, pralatrexate, prednimustine, prednisone, procarbazine, procodazole, propranolol, quinagolide, rabeprazole, racotumomab, radium-223 chloride, radotinib, raloxifene, raltitrexed, ramosetron, ramucirumab, ranimustine, rasburicase, razoxane, refametinib, regorafenib, ribociclib, risedronic acid, rhenium-186 etidronate, rituximab, rolapitant, romidepsin, romiplostim, romurtide, rucaparib, samarium (153Sm) lexidronam, sargramostim, sarilumab, satumomab, secretin, siltuximab, sipuleucel-T, sizofiran, sobuzoxane, sodium glycididazole, sonidegib, sorafenib, stanozolol, streptozocin, sunitinib, talaporfin, talimogene laherparepvec, tamibarotene, tamoxifen, tapentadol, tasonermin, teceleukin, technetium (99 mTc) nofetumomab merpentan, 99 mTc-HYNIC-[Tyr3]-octreotide, tegafur, tegafur+gimeracil+oteracil, temoporfin, temozolomide, temsirolimus, teniposide, testosterone, tetrofosmin, thalidomide, thiotepa, thymalfasin, thyrotropin alfa, tioguanine, tisagenlecleucel, tislelizumab, tocilizumab, topotecan, toremifene, tositumomab, trabectedin, trametinib, tramadol, trastuzumab, trastuzumab emtansine, treosulfan, tretinoin, trifluridine+tipiracil, trilostane, triptorelin, trametinib, trofosfamide, thrombopoietin, tryptophan, ubenimex, valatinib, valrubicin, vandetanib, vapreotide, vemurafenib, vinblastine, vincristine, vindesine, vinflunine, vinorelbine, vismodegib, vorinostat, vorozole, yttrium-90 glass microspheres, zinostatin, zinostatin stimalamer, zoledronic acid, zorubicin.

The compounds of the invention can further be combined with other reagents targeting the immune system, such as immune checkpoint inhibitors, e.g. aPD-1/-L1 axis antagonists. PD-1, along with its ligands PD-L1 and PD-L2, function as negative regulators of T cell activation. PD-L1 is overexpressed in many cancers and overexpression of PD-1 often occurs concomitantly in tumor infiltrating T cells. This results in attenuation of T cell activation and evasion of immune surveillance, which contributes to impaired antitumor immune responses. (M. E. Keir et al., Annu. Rev. Immunol. 2008, 26, 677-704).

In accordance with a further aspect, the present invention covers combinations comprising one or more of the compounds of general formula (I), as described herein, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, and one or more immune checkpoint inhibitors. Preferably, the immune checkpoint inhibitor is a aPD-1/-L1 axis antagonist.

The compounds of the invention can further be combined with inhibitors of DGKα, such as those inhibitors of DGKα disclosed in WO2020/006016, WO2020/006018 and WO 2021/041588. As DGKα in T cells operates in a similar fashion as DGKζ, a dual inhibition profoundly enhances T cell effector functions compared with cells with deletion of either DGKζ isoform alone or wild-type cells. (M. J. Riese et al. Cancer Res. (2013), 73 (12); p. 3566-77).

The compounds of the invention can further be combined with chimeric antigen receptor T cells (CAR-T cells), such as Axicabtagen-Ciloleucel or Tisagenlecleucel. The activity of CAR-T cells can be suppressed by the tumor micro environment (TME). Knock out of DGKs by techniques such as Crispr had been shown to enhance CAR-T cell activity in a suppressive TME (I. Y. Jung et al., Mol. Cells 2018, 41 (8), 717-723).

In accordance with a further aspect, the present invention covers combinations comprising one or more compounds of general formula (I), as described herein, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, with chimeric antigen receptor T cells, (CAR-T cells), CAR-NKT cells or CAR-NK cells.

Preferably, the chimeric antigen receptor T cells (CAR-T cells) are Axicabtagen-Ciloleucel or Tisagenlecleucel.

The present invention further provides the use of the compounds according to the invention for expansion of T cells including CAR-T and tumor infiltrated lymphocytes ex-vivo.

In accordance with a further aspect, the present invention covers compounds of general formula (I), as described herein, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, for use in the expansion of T cells including CAR-T cells, CAR-NKT cells or CAR-NK cells and tumor infiltrated lymphocytes ex-vivo.

Hence, the present invention also relates to the use of the compounds according to the invention for the expansion of T cells, including CAR-T cell, CAR-NKT cells or CAR-NK cells and tumor infiltrated lymphocytes, ex-vivo.

The present invention also comprises an ex-vivo method for the expansion of T cells, including CAR-T cells, CAR-NKT cells or CAR-NK cells and tumor infiltrated lymphocytes, contacting said T cells with compounds according to the invention.

Compounds of the present invention can be utilized to inhibit, block, reduce or decrease DGKζ activity resulting in the modulation of dysregulated immune responses e.g. to block immunosuppression and increase immune cell activation and infiltration in the context of cancer and cancer immunotherapy that will eventually lead to reduction of tumour growth.

This method comprises administering to a mammal in need thereof, including a human, an amount of a compound of this invention, or a pharmaceutically acceptable salt, isomer, polymorph, metabolite, hydrate, solvate or ester thereof; which is effective to treat the disorder. The present invention also provides methods of treating a variety of other disorders wherein DGKζ is involved such as, but not limited to, disorders with dysregulated immune responses, inflammation, vaccination for infection & cancer, virus infections, lymphoproliferative disorders, asthma, eye diseases, and type 2 diabetes/insulin resistance.

These disorders have been well characterized in humans, but also exist with a similar etiology in other mammals, and can be treated by administering pharmaceutical compositions of the present invention.

In accordance with a further aspect, the present invention covers compounds of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, for use in the treatment or prophylaxis of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling.

The pharmaceutical activity of the compounds according to the invention can be explained by their activity as DGKζ inhibitors.

In accordance with a further aspect, the present invention covers the use of compounds of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, for the treatment or prophylaxis of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours.

In accordance with a further aspect, the present invention covers the compounds of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, for the use in the treatment or prophylaxis of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours.

In accordance with a further aspect, the present invention covers the use of compounds of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, in a method of treatment or prophylaxis of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours.

In accordance with a further aspect, the present invention covers the use of a compound of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, in a method of treatment or prophylaxis of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours.

In accordance with a further aspect, the present invention covers use of a compound of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same, for the preparation of a pharmaceutical composition, preferably a medicament, for the prophylaxis or treatment of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours.

In accordance with a further aspect, the present invention covers a method of treatment or prophylaxis of diseases, in particular cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours, using an effective amount of a compound of general formula (I), as described supra, or stereoisomers, tautomers, N-oxides, hydrates, solvates, and salts thereof, particularly pharmaceutically acceptable salts thereof, or mixtures of same.

In accordance with a further aspect, the present invention covers pharmaceutical compositions, in particular a medicament, comprising a compound of general formula (I), as described supra, or a stereoisomer, a tautomer, an N-oxide, a hydrate, a solvate, a salt thereof, particularly a pharmaceutically acceptable salt, or a mixture of same, and one or more excipients), in particular one or more pharmaceutically acceptable excipient(s). Conventional procedures for preparing such pharmaceutical compositions in appropriate dosage forms can be utilized.

The present invention furthermore covers pharmaceutical compositions, in particular medicaments, which comprise at least one compound according to the invention, conventionally together with one or more pharmaceutically suitable excipients, and to their use for the above mentioned purposes.

It is possible for the compounds according to the invention to have systemic and/or local activity. For this purpose, they can be administered in a suitable manner, such as, for example, via the oral, parenteral, pulmonary, nasal, sublingual, lingual, buccal, rectal, vaginal, dermal, transdermal, conjunctival, otic route or as an implant or stent.

For these administration routes, it is possible for the compounds according to the invention to be administered in suitable administration forms.

For oral administration, it is possible to formulate the compounds according to the invention to dosage forms known in the art that deliver the compounds of the invention rapidly and/or in a modified manner, such as, for example, tablets (uncoated or coated tablets, for example with enteric or controlled release coatings that dissolve with a delay or are insoluble), orally-disintegrating tablets, films/wafers, films/lyophilisates, capsules (for example hard or soft gelatine capsules), sugar-coated tablets, granules, pellets, powders, emulsions, suspensions, aerosols or solutions. It is possible to incorporate the compounds according to the invention in crystalline and/or amorphised and/or dissolved form into said dosage forms.

Parenteral administration can be effected with avoidance of an absorption step (for example intravenous, intraarterial, intracardial, intraspinal or intralumbal) or with inclusion of absorption (for example intramuscular, subcutaneous, intracutaneous, percutaneous or intraperitoneal). Administration forms which are suitable for parenteral administration are, inter alia, preparations for injection and infusion in the form of solutions, suspensions, emulsions, lyophilisates or sterile powders.

Examples which are suitable for other administration routes are pharmaceutical forms for inhalation [inter alia powder inhalers, nebulizers], nasal drops, nasal solutions, nasal sprays; tablets/films/wafers/capsules for lingual, sublingual or buccal administration; suppositories; eye drops, eye ointments, eye baths, ocular inserts, ear drops, ear sprays, ear powders, ear-rinses, ear tampons; vaginal capsules, aqueous suspensions (lotions, mixturae agitandae), lipophilic suspensions, emulsions, ointments, creams, transdermal therapeutic systems (such as, for example, patches), milk, pastes, foams, dusting powders, implants or stents.

The compounds according to the invention can be incorporated into the stated administration forms. This can be effected in a manner known per se by mixing with pharmaceutically suitable excipients. Pharmaceutically suitable excipients include, inter alia,

-   -   fillers and carriers (for example cellulose, microcrystalline         cellulose (such as, for example, Avicel®), lactose, mannitol,         starch, calcium phosphate (such as, for example, Di-Cafos®)),     -   ointment bases (for example petroleum jelly, paraffins,         triglycerides, waxes, wool wax, wool wax alcohols, lanolin,         hydrophilic ointment, polyethylene glycols),     -   bases for suppositories (for example polyethylene glycols, cacao         butter, hard fat),     -   solvents (for example water, ethanol, isopropanol, glycerol,         propylene glycol, medium chain-length triglycerides fatty oils,         liquid polyethylene glycols, paraffins),     -   surfactants, emulsifiers, dispersants or wetters (for example         sodium dodecyl sulfate), lecithin, phospholipids, fatty alcohols         (such as, for example, Lanette®), sorbitan fatty acid esters         (such as, for example, Span®), polyoxyethylene sorbitan fatty         acid esters (such as, for example, Tween®), polyoxyethylene         fatty acid glycerides (such as, for example, Cremophor®),         polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol         ethers, glycerol fatty acid esters, poloxamers (such as, for         example, Pluronic®),     -   buffers, acids and bases (for example phosphates, carbonates,         citric acid, acetic acid, hydrochloric acid, sodium hydroxide         solution, ammonium carbonate, trometamol, triethanolamine),     -   isotonicity agents (for example glucose, sodium chloride),     -   adsorbents (for example highly-disperse silicas),     -   viscosity-increasing agents, gel formers, thickeners and/or         binders (for example polyvinylpyrrolidone, methylcellulose,         hydroxypropylmethylcellulose, hydroxypropylcellulose,         carboxymethylcellulose-sodium, starch, carbomers, polyacrylic         acids (such as, for example, Carbopol®); alginates, gelatine),     -   disintegrants (for example modified starch,         carboxymethylcellulose-sodium, sodium starch glycolate (such as,         for example, Explotab®), cross-linked polyvinylpyrrolidone,         croscarmellose-sodium (such as, for example, AcDiSol®)),     -   flow regulators, lubricants, glidants and mould release agents         (for example magnesium stearate, stearic acid, talc,         highly-disperse silicas (such as, for example, Aerosil®)),     -   coating materials (for example sugar, shellac) and film formers         for films or diffusion membranes which dissolve rapidly or in a         modified manner (for example polyvinylpyrrolidones (such as, for         example, Kollidon®), polyvinyl alcohol,         hydroxypropylmethylcellulose, hydroxypropylcellulose,         ethylcellulose, hydroxypropylmethylcellulose phthalate,         cellulose acetate, cellulose acetate phthalate, polyacrylates,         polymethacrylates such as, for example, Eudragit®)),     -   capsule materials (for example gelatine,         hydroxypropylmethylcellulose),     -   synthetic polymers (for example polylactides, polyglycolides,         polyacrylates, polymethacrylates (such as, for example,         Eudragit®), polyvinylpyrrolidones (such as, for example,         Kollidon®), polyvinyl alcohols, polyvinyl acetates, polyethylene         oxides, polyethylene glycols and their copolymers and         blockcopolymers),     -   plasticizers (for example polyethylene glycols, propylene         glycol, glycerol, triacetine, triacetyl citrate, dibutyl         phthalate),     -   penetration enhancers,     -   stabilisers (for example antioxidants such as, for example,         ascorbic acid, ascorbyl palmitate, sodium ascorbate,         butylhydroxyanisole, butylhydroxytoluene, propyl gallate),     -   preservatives (for example parabens, sorbic acid, thiomersal,         benzalkonium chloride, chlorhexidine acetate, sodium benzoate),     -   colourants (for example inorganic pigments such as, for example,         iron oxides, titanium dioxide),     -   flavourings, sweeteners, flavour- and/or odour-masking agents.

The present invention furthermore relates to a pharmaceutical composition which comprise at least one compound according to the invention, conventionally together with one or more pharmaceutically suitable excipient(s), and to their use according to the present invention.

In accordance with another aspect, the present invention covers pharmaceutical combinations, in particular medicaments, comprising at least one compound of general formula (I) of the present invention and at least one or more further active ingredients, in particular for the treatment and/or prophylaxis of cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, particularly liquid and solid tumours.

Particularly, the present invention covers a pharmaceutical combination, which comprises:

-   -   one or more first active ingredients, in particular compounds of         general formula (I) as defined supra, and     -   one or more further active ingredients, in particular in         particular immune checkpoint inhibitors.

The term “combination” in the present invention is used as known to persons skilled in the art, it being possible for said combination to be a fixed combination, a non-fixed combination or a kit-of-parts.

A “fixed combination” in the present invention is used as known to persons skilled in the art and is defined as a combination wherein, for example, a first active ingredient, such as one or more compounds of general formula (I) of the present invention, and a further active ingredient are present together in one unit dosage or in one single entity. One example of a “fixed combination” is a pharmaceutical composition wherein a first active ingredient and a further active ingredient are present in admixture for simultaneous administration, such as in a formulation. Another example of a “fixed combination” is a pharmaceutical combination wherein a first active ingredient and a further active ingredient are present in one unit without being in admixture.

A non-fixed combination or “kit-of-parts” in the present invention is used as known to persons skilled in the art and is defined as a combination wherein a first active ingredient and a further active ingredient are present in more than one unit. One example of a non-fixed combination or kit-of-parts is a combination wherein the first active ingredient and the further active ingredient are present separately. It is possible for the components of the non-fixed combination or kit-of-parts to be administered separately, sequentially, simultaneously, concurrently or chronologically staggered.

Based upon standard laboratory techniques known to evaluate compounds useful for the treatment of cancer or conditions with dysregulated immune responses or other disorders associated with aberrant DGKζ signaling, by standard toxicity tests and by standard pharmacological assays for the determination of treatment of the conditions identified above in mammals, and by comparison of these results with the results of known active ingredients or medicaments that are used to treat these conditions, the effective dosage of the compounds of the present invention can readily be determined for treatment of each desired indication. The amount of the active ingredient to be administered in the treatment of one of these conditions can vary widely according to such considerations as the particular compound and dosage unit employed, the mode of administration, the period of treatment, the age and sex of the patient treated, and the nature and extent of the condition treated.

The total amount of the active ingredient to be administered will generally range from about 0001 mg/kg to about 200 mg/kg body weight per day, and preferably from about 0.01 mg/kg to about 20 mg/kg body weight per day. Clinically useful dosing schedules will range from one to three times a day dosing to once every four weeks dosing. In addition, it is possible for “drug holidays”, in which a patient is not dosed with a drug for a certain period of time, to be beneficial to the overall balance between pharmacological effect and tolerability. It is possible for a unit dosage to contain from about 0.5 mg to about 1500 mg of active ingredient, and can be administered one or more times per day or less than once a day. The average daily dosage for administration by injection, including intravenous, intramuscular, subcutaneous and parenteral injections, and use of infusion techniques will preferably be from 0.01 to 200 mg/kg of total body weight. The average daily rectal dosage regimen will preferably be from 0.01 to 200 mg/kg of total body weight. The average daily vaginal dosage regimen will preferably be from 0.01 to 200 mg/kg of total body weight. The average daily topical dosage regimen will preferably be from 0.1 to 200 mg administered between one to four times daily. The transdermal concentration will preferably be that required to maintain a daily dose of from 0.01 to 200 mg/kg. The average daily inhalation dosage regimen will preferably be from 0.01 to 100 mg/kg of total body weight.

Of course the specific initial and continuing dosage regimen for each patient will vary according to the nature and severity of the condition as determined by the attending diagnostician, the activity of the specific compound employed, the age and general condition of the patient, time of administration, route of administration, rate of excretion of the drug, drug combinations, and the like. The desired mode of treatment and number of doses of a compound of the present invention or a pharmaceutically acceptable salt or ester or composition thereof can be ascertained by those skilled in the art using conventional treatment tests.

General Syntheses of Compounds of the Present Invention

The compounds according to the invention of general formula (I) can be prepared according to the following Schemes 1, 2, 3 and 4. The schemes and procedures described below illustrate synthetic routes to the compounds of general formula (I) of the invention and are not intended to be limiting. It is clear to the person skilled in the art that the order of transformations as exemplified in Schemes 1, 2, 3 and 4 can be modified in various ways. The order of transformations exemplified in these schemes is therefore not intended to be limiting. In addition, modification of any of the substituents, R¹, R², R³ or R⁴ can be achieved before and/or after the exemplified transformations. These modifications can be such as the introduction of protecting groups, cleavage of protecting groups, reduction or oxidation of functional groups, halogenation, metallation, substitution, or coupling reactions such as amide couplings (couplings of carboxylic acids with amines) or transition metal catalysed coupling reactions (such as the well-known Suzuki coupling) known to the person skilled in the art. These transformations include those which introduce a functionality which allows for further modification of substituents. Appropriate protecting groups and their introduction and cleavage are well-known to the person skilled in the art (see for example T. W. Greene and P. G. M. Wuts in Protective Groups in Organic Synthesis, 3^(rd) edition, Wiley 1999). Specific examples are described in the subsequent paragraphs.

Suitable routes for the preparation of compounds of formulae (I-a) and (I-b), which are sub-sets of general formula (I), which when taken together they form, and for the preparation of compounds of formula (I-c) which, in turn constitutes a sub-set of formula (I-a), are described in Schemes 1, 2, 3 and 4.

Compounds of formula (I-a), which constitutes a sub-set of general formula (I) in which R³ represents an amino group, can be prepared from isothiocyanates of formula (II), in which R¹ has the meaning as given for general formula (I), and ketones of formula (III), in which R⁴ has the meaning as given for general formula (I), and in which LG¹ represents a leaving group as defined herein, preferably a chlorine, bromine, or iodine atom, more preferably a bromine atom, by reacting with cyanamide (IV), in the presence of a non-nucleophilic base, preferably 1,8-diazabicyclo(5.4.0)undec-7-ene (herein also being referred to as DBU, CAS-RN 6674-22-2), in a dipolar aprotic solvent as defined herein, preferably acetonitrile, at a temperature in the range from 0° C. to 50° C., preferably 15° C. to 30° C., more preferably at room temperature, for a time in the range from 1 hour to 100 hours, preferably from 1 hour to 10 hours, more preferably from 2 hours to 4 hours, to give intermediate compounds of formula (V-a). Said intermediate compounds of formula (V-a) can subsequently be reacted with compounds of formula (VI), in which R² has the meaning as given for general formula (I), and in which LG² represents a leaving group as defined herein, preferably a chlorine, bromine, or iodine atom, or a (methylsulfonyl)oxy or [(4-methylphenyl)sulfonyl]oxy group, more preferably a bromine atom, in a dipolar aprotic solvent as defined herein, preferably N,N-dimethylformamide, at a temperature in the range from 60° C. to 120° C., preferably 80° C. to 100° C., more preferably 90° C., for a time in the range from 30 minutes to 24 hours, preferably from 1 hour to 4 hours, more preferably 2 hours, to give compounds of the present invention, of formula (I-a). Said conversion of intermediate compounds of formula (V-a) into compounds of the present invention of formula (I-a) by reacting with compounds of formula (VI) can also be accomplished advantageously in a dipolar aprotic solvent as defined herein, preferably N,N-dimethylformamide, at a temperature in the range from 0° C. to 60° C., preferably 10° C. to 40° C., more preferably at room temperature as defined herein, for a time in the range from 6 hours to 48 hours, preferably from 12 hour to 24 hours. Specific examples are described in the Experimental Section. As indicated in the introductory paragraph of this section, substituents, e.g. those attached to R¹ and R⁴, can be modified by various methods known to the person skilled in the art during this synthesis route or on the final step. Specific examples are described in the Experimental Section.

The reaction between intermediates of the formula (V-a) and compounds of formula (VI) proceeds selectively at the nitrogen atom attached to C-2 of the thiazole, not involving the —NH₂ group attached to C₁₋₄. As shown in Scheme 2, this also applies to the analogous reaction with di-tert-butyl dicarbonate which gives compounds of formula (V-c) but not compounds of formula (V-b), said compounds of formula (V-c) being converted, upon reaction with compounds of formula (VI), in which R² has the meaning as given for general formula (I), and in which LG² represents a leaving group as defined herein, preferably a chlorine, bromine, or iodine atom, more preferably a bromine atom, in a dipolar aprotic solvent as defined herein, preferably N,N-dimethylformamide, at a temperature in the range from 60° C. to 120° C., preferably 80° C. to 100° C., more preferably 90° C., for a time in the range from 30 minutes to 24 hours, preferably from 1 hour to 4 hours, more preferably 2 hours, into compounds of the present invention, of formula (I-a), likewise. Specific examples are described in the Experimental Section.

Compounds of formula (I-b), which constitutes a sub-set of general formula (I) in which R³ represents a methyl group, can be prepared from isothiocyanates of formula (II), in which R¹ has the meaning as given for general formula (I), and ketones of formula (III), in which R⁴ has the meaning as given for general formula (I), and in which LG¹ represents a leaving group as defined herein, preferably a chlorine, bromine, or iodine atom, more preferably a bromine atom, by reacting with a salt of ethanimidamide of formula (VII), in which X⁻ represents a monovalent anion, preferably chloride, bromide, iodide or acetate, more preferably chloride, in the presence of a non-nucleophilic base, preferably 1,8-diazabicyclo(5.4.0)undec-7-ene (herein also being referred to as DBU, CAS-RN 6674-22-2), in a dipolar aprotic solvent as defined herein, preferably acetonitrile, at a temperature in the range from 0° C. to 50° C., preferably 15° C. to 30° C., more preferably at room temperature, for a time in the range from 1 hour to 100 hours, preferably from 1 hour to 10 hours, more preferably from 2 hours to 4 hours, to give intermediate compounds of formula (V-d). Said intermediate compounds of formula (V-d) can subsequently be reacted with compounds of formula (VI), in which R² has the meaning as given for general formula (I), and in which LG² represents a leaving group as defined herein, preferably a chlorine, bromine, or iodine atom, or a (methylsulfonyl)oxy or [(4-methylphenyl)sulfonyl]oxy group, more preferably a bromine atom, in a dipolar aprotic solvent as defined herein, preferably N,N-dimethylformamide, at a temperature in the range from 60° C. to 120° C., preferably 80° C. to 100° C., more preferably 90° C., for a time in the range from 30 minutes to 24 hours, preferably from 1 hour to 4 hours, more preferably 2 hours, to give compounds of the present invention, of formula (I-b). Specific examples are described in the Experimental Section.

Compounds of formulae (II), (III), (IV), (VI), and (VII) are largely commercially available or can be prepared using methods known to the person skilled in the art, see e.g. F. Calderon et al. Journal of Medicinal Chemistry 2017, 60 (16), 6880-6896, for the preparation of isothiocyanates of formula (II); see also the synthesis protocols of Intermediates 200-208 in the Experimental Section, or Y. Xing et al. European Journal of Organic Chemistry 2017, 2017 (4), 781-785; A. Wu et al. Tetrahedron 2013, 69 (31), 6392-6398, for the preparation of ketones of formula (III); see also the synthesis protocols of Intermediates 198 and 199 in the Experimental Section.

Alternatively, compounds of formula (I-a), which constitutes a sub-set of general formula (I) in which R³ represents an amino group, can be prepared from precursors in which the R⁴ group substituted with a group not covered within the definition of R⁴ (such as a carboxy group), which however allows for establishing R⁴ groups featuring diverse substituents from a common precursor, such as carboxamides of formula (I-c) (Scheme 4).

Scheme 4 exemplifies (without any limitation to the invention) the conversions of advanced intermediates of formula (VIII), which can be prepared using the methods described in the preceding Schemes, in combination with well-established methods such as the saponification of carboxylic esters, Thus, carboxylic acid derivatives of formula (VIII) can be reacted in well-known amide coupling reactions with amines (R¹²)(R¹³)NH, in the presence of a coupling reagent such as HATU and a tertiary amine such as triethylamine or N,N-diisopropylethylamine, and optionally in the presence of DMAP, in a dipolar aprotic solvent as defined herein, preferably N,N-dimethylformamide, at a temperature in the range from 0° C. to 50° C., preferably at room temperature as defined herein, for a time in the range from 2 hours to 24 hours, preferably from 12 hour to 20 hours, to give carboxamide compounds of the invention, of formula (I-c). Specific examples are described in the Experimental Section.

In accordance with a second aspect, the present invention covers methods of preparing compounds of formulae (I-a) and (I-b), which are sub-sets of general formula (I), supra, which when taken together they form.

In accordance with a second embodiment of the second aspect, said methods comprise the step of allowing an intermediate compound of formula (V-a):

in which R¹ and R⁴ are as defined for the compound of general formula (I) as defined supra, to react with a compound of formula (VI):

in which R² is as defined for the compound of general formula (I) as defined supra, and in which LG² represents a leaving group as defined herein, thereby giving a compound of formula (I-a)

in which R¹, R² and R⁴ are as defined supra.

In accordance with a third embodiment of the second aspect, the present invention covers methods of preparing compounds of formula (I-a), said methods comprising the step of allowing an intermediate compound of formula (V-c):

in which R¹ and R⁴ are as defined for the compound of general formula (I) as defined supra, to react with a compound of formula (VI):

in which R² is as defined for the compound of general formula (I) as defined supra, and in which LG² represents a leaving group as defined herein, thereby giving a compound of formula (I-a)

in which R¹, R² and R⁴ are as defined supra.

In accordance with a fourth embodiment of the second aspect, the present invention covers methods of preparing compounds of formula (I-b), said methods comprising the step of allowing an intermediate compound of formula (V-d):

in which R¹ and R⁴ are as defined for the compound of general formula (I) as defined supra, to react with a compound of formula (VI):

in which R² is as defined for the compound of general formula (I) as defined supra, and in which LG² represents a leaving group as defined herein, thereby giving a compound of formula (I-b):

in which R¹, R² and R⁴ are as defined supra.

In accordance with a third aspect, the present invention covers methods of preparing compounds of formulae (I-a) and (I-b), which are sub-sets of general formula (I), supra, which when taken together they form.

In accordance with a second embodiment of the third aspect, said methods comprise the step of allowing an intermediate compound of formula (V-a)

in which R¹ and R⁴ are as defined for the compound of general formula (I) as defined supra, to react with a compound of formula (VI):

in which R² is as defined for the compound of general formula (I) as defined supra, and in which LG² represents a leaving group as defined herein, thereby giving a compound of formula (I-a):

in which R¹, R² and R⁴ are as defined supra, then optionally converting said compound into solvates, salts and/or solvates of such salts using the corresponding (i) solvents and/or (ii) bases or acids.

In accordance with a third embodiment of the third aspect, the present invention covers methods of preparing compounds of formula (I-a), said methods comprising the step of allowing an intermediate compound of formula (V-c):

in which R¹ and R⁴ are as defined for the compound of general formula (I) as defined supra, to react with a compound of formula (VI):

in which R² is as defined for the compound of general formula (I) as defined supra, and in which LG² represents a leaving group as defined herein, thereby giving a compound of formula (I-a):

in which R¹, R² and R⁴ are as defined supra, then optionally converting said compound into solvates, salts and/or solvates of such salts using the corresponding (i) solvents and/or (ii) bases or acids.

In accordance with a fourth embodiment of the third aspect, the present invention covers methods of preparing compounds of formula (I-b), said methods comprising the step of allowing an intermediate compound of formula (V-d):

in which R¹ and R⁴ are as defined for the compound of general formula (I) as defined supra, to react with a compound of formula (VI):

in which R² is as defined for the compound of general formula (I) as defined supra, and in which LG² represents a leaving group as defined herein, thereby giving a compound of formula (I-b):

in which R¹, R² and R⁴ are as defined supra,

then optionally converting said compound into solvates, salts and/or solvates of such salts using the corresponding (i) solvents and/or (ii) bases or acids.

The present invention covers methods of preparing compounds of the present invention of general formula (I), said methods comprising the steps as described in the Experimental Section herein.

In accordance with a fourth aspect, the present invention covers intermediate compounds which are useful for the preparation of the compounds of formulae (I-a) and (I-b), which are sub-sets of general formula (I), supra, which when taken together they form.

In accordance with a second embodiment of the fourth aspect, the present invention covers the intermediate compounds of formula (V-c)

in which R¹ and R⁴ are as defined for the compound of general formula (I) supra.

In accordance with a third embodiment of the fourth aspect, the present invention covers the intermediate compounds of formula (V-d):

in which R¹ and R⁴ are as defined for the compound of general formula (I) supra.

In accordance with a fifth aspect, the present invention covers the use of intermediate compounds for the preparation of the compounds of formulae (I-a) and (I-b), which are sub-sets of general formula (I), supra, which when taken together they form.

In accordance with a second embodiment of the fifth aspect, the present invention covers the intermediate compounds of formula (V-a):

in which R¹ and R⁴ are as defined for the compound of general formula (I) supra, for the preparation of a compound of formula (I-a) as defined supra.

In accordance with a third embodiment of the fifth aspect, the present invention covers the intermediate compounds of formula (V-c)

in which R¹ and R⁴ are as defined for the compound of general formula (I) supra, for the preparation of a compound of formula (I-a) as defined supra.

In accordance with a fourth embodiment of the fifth aspect, the present invention covers the intermediate compounds of formula (V-d)

in which R¹ and R⁴ are as defined for the compound of general formula (I) supra, for the preparation of a compound of formula (I-b) as defined supra.

The present invention covers intermediate compounds which are disclosed in the Example Section of this text, infra.

The present invention covers the use of intermediate compounds which are disclosed in the Example Section of this text, infra.

The present invention covers any sub-combination within any embodiment or aspect of the present invention of intermediate compounds of formulae (V-a), (V-c) and (V-d), supra.

DESCRIPTION OF THE FIGURES

FIG. 1 : DGKz_hu_1 encoding human DGKζ M1 to V928 plus N-terminal Flag-Tag, as described under SEQ ID No. 1.

FIG. 2 : SIINFEKL amino acid sequence, as described under SEQ ID No. 2.

FIG. 3 : OVA-30 peptide sequence, as described under SEQ ID No. 3.

FIG. 4 : FLAG-Tag, as described under SEQ ID No. 4.

FIG. 5 : Kozak sequence for translation initiation, as described under SEQ ID No. 5.

FIG. 6 : 50% thermal ellipsoids of Example 49.2, Molecule 1. F171 and F151 represents the two refined positions for the 180° disorder over the C13-C16 axis.

FIG. 7 : 50% thermal ellipsoids of Example 59.1, Molecule 1 FIG. 8 : 50% thermal ellipsoids of Example 61.2, Molecule 1 FIG. 9 : 50% thermal ellipsoids of Example 62.2, Molecule 1

EXPERIMENTAL SECTION—GENERAL PART

NMR peak forms are stated as they appear in the spectra, possible higher order effects have not been considered. The multiplicities are stated according to the signal form which appears in the spectrum, NMR-spectroscopic effects of a higher order were not taken into consideration.

Multiplicity of the NMR signals: s=singlet, d=doublet, t=triplet, q=quartet, quin=quintet, br=broad signal, m=multiplet. NMR signals: shift in [ppm]. Combinations of multiplicity could be e.g. dd=doublet from doublet.

Chemical names were generated using software programs such as the ACD/Name batch version 14.05 from ACD/Labs and BioVia Draw 2019 Version 19.1 NET, and chemical names were adapted if needed. In some cases generally accepted names of commercially available reagents were used in place of chemical names generated using abovementioned software programs.

All reagents the synthesis of which is not described in the experimental part were purchased commercially, or said reagents are known compounds or can be formed from known compounds by known methods by a person skilled in the art.

Table 1 lists the abbreviations used in this paragraph and in the Examples section as far as they are not explained within the text body. Other abbreviations have their meanings customary per se to the skilled person.

TABLE 1 Abbreviations CDCl₃ deuterochloroform DAD diode array detector SQD single quadrupole detector Azura UVD single variable wavelength UV detector for HPLC DMF N,N-dimethylformamide DMSO-d6 deuterated dimethyl sulphoxide DMSO dimethyl sulphoxide ELSD evaporative light scattering detector ESIpos electrospray ionization positive Expl. example HATU (7-aza-1H-benzotriazol-1-yl)-1,1,3,3- tetramethyluronium hexafluorophosphate HPLC high-pressure liquid chromatography LC-MS liquid chromatography coupled with mass spectrometry mL milliliter min minute(s) MTBE methyl tert-butyl ether RP-HPLC reverse-phase high-pressure liquid chromatography Rt retention time rt room temperature sat. saturated THF tetrahydrofurane EtOAc ethyl acetate TLC thin layer chromatography rac racemic μM micromolar M molar UPLC Ultra high performance chromatography UPLC-MS Ultra high performance chromatography coupled with mass spectrometry BEH ethylene bridged hybrid CSH charged surface hybrid UV ultra violet CAS-RN chemical abstracts service registry number NMR nuclear magnetic resonance MHz Megahertz

Analytical UPLC-MS Standard Procedures

Method 1/acidic

-   -   Instrument: Waters Acquity UPLC-MS SQD 3001; column: Acquity         UPLC BEH C18 1.7 50×2.1 mm; eluent A: water+0.1 vol % formic         acid, eluent B: acetonitrile; gradient: 0-1.6 min 1-99% B,         1.6-2.0 min 99% B; flow 0.8 mL/min; temperature: 60° C.;         injection: 2 μL; DAD scan: 210-400 nm; ELSD

Method 2/Basic

-   -   Instrument: Waters Acquity UPLC-MS SQD 3001; column: Acquity         UPLC BEH C18 1.7 50×2.1 mm; eluent A: water+0.2 vol % aqueous         ammonia (32%), eluent B: acetonitrile; gradient: 0-1.6 min 1-99%         B, 1.6-2.0 min 99% B; flow 0.8 mL/min; temperature: 60° C.;         injection: 2 μL; DAD scan: 210-400 nm; ELSD

Method 3/Basic

-   -   Instrument: Waters Acquity UPLC-MS SingleQuad; Column: Acquity         UPLC CSH C18 1.7 μm 50×2.1 mm; eluent A: water+0.2 vol % aqueous         ammonia (32%), eluent B: acetonitrile; gradient: 0-1.6 min 1-99%         B, 1.6-2.0 min 99% B; flow 0.8 mL/min; temperature: 60° C.; DAD         scan: 210-400 nm.

Optical Rotation

Optical rotations were measured with a JASCO Polarimeter 2000 using the solvent and concentration stated in each case at 20° C., wavelength 589 nm, integration time 10 s, layer thickness 100 mm.

Compound Purification—General

The example compounds and intermediates produced according to the methods of the invention may require purification. Purification of organic compounds is well known to the person skilled in the art and there may be several ways of purifying the same compound. In some cases, no purification may be necessary. In some cases, the compounds may be purified by crystallization.

In some cases, impurities may be stirred out using a suitable solvent. In some cases, the compounds may be purified by chromatography, particularly flash column chromatography, using for example prepacked silica gel cartridges, e.g. Biotage SNAP cartidges KP-Sil® or KP-NH® in combination with a Biotage autopurifier system (SP4® or Isolera Four®) and eluents such as gradients of hexane/ethyl acetate or dichloromethane/methanol. In some cases, the compounds may be purified by preparative HPLC using for example a Waters autopurifier system equipped with a diode array detector and/or on-line electrospray ionization mass spectrometer in combination with a suitable prepacked reverse phase column and eluents such as gradients of water and acetonitrile which may contain additives such as trifluoroacetic acid, formic acid or aqueous ammonia.

In some cases, purification methods as described above can provide those compounds of the present invention which possess a sufficiently basic or acidic functionality in the form of a salt, such as, in the case of a compound of the present invention which is sufficiently basic, a trifluoroacetate or formate salt for example, or, in the case of a compound of the present invention which is sufficiently acidic, an ammonium salt for example. A salt of this type can either be transformed into its free base or free acid form, respectively, by various methods known to the person skilled in the art, or be used as salts in subsequent biological assays. It is to be understood that the specific form (e.g. salt, free base etc.) of a compound of the present invention as isolated and as described herein is not necessarily the only form in which said compound can be applied to a biological assay in order to quantify the specific biological activity.

Specific methods are described below, and in the respective protocols describing the preparations of example compounds and intermediates.

Preparative HPLC

Instrument: pump: Labomatic HD-3000, head HDK 280, low pressure gradient module ND-B1000; manual injection valve: Rheodyne 3725i038; detector: Knauer Azura UVD 2.15; collector: Labomatic Labocol Vario-4000; column: Chromatorex RP C-18 10 μm, 125×30 mm; eluent; gradient; UV-Detection. Eluent: solvent A: water+0.1 vol % formic acid (99%; acidic) or 0.2 vol % aqueous ammonia (32%, basic), solvent B: acetonitrile; flow 150 mL/min.

Gradients:

Method A: 0.00-0.50 min 1% B, 0.50-600 min 1-25% B, 6.00-6.10 min 25-100% B, 6.10-8.00 min 100% B

Method B: 0.00-0.50 min 10% B, 0.50-6.00 min 10-50% B. 6.00-6.10 min 50-100% B. 6.10-8.00 min 100% B

Method C: 0.00-0.50 min 15% B, 0.50-6.00 min 15-55% B, 6.00-6.10 min 55-100% B, 6.10-8.00 min 100% B

Method D: 0.00-0.50 min 30% B, 0.50-6.00 min 30-70% B, 6.00-6.10 min 70-100% B, 6.10-8.00 min 100% B

Method E: 0.00-0.50 min 40% B, 0.50-6.00 min 40-80% B, 6.00-6.10 min 80-100% B, 6.10-8.00 min 100% B

Method F:

Instrument: Waters autopurification system; column: Waters CSH C18 5μ 100×30 mm; eluent A: water+0.1 Vol-% aqueous ammonia (32%), eluent B: acetonitrile, DAD scan: 210-400 nm; MS Instrument: QDA (Waters); Collector-Trigger: DAD-MS flow rate: 60 mL/min

Chiral HPLC and Stereochemistry Assignments

Separations of stereoisomeric mixtures, such as racemic compounds, by chiral HPLC can result in the isolation of single stereoisomers without known configuration of the respective stereogenic centres in the isolated isomers. In the following, the full chemical names of all such separated isomers obtained from an isomeric mixture, including (R) and (S) configurations, are listed in alphabetical order together with all respective intermediate or example numbers, followed by the individual isomers with data on their analytics, isolation and yield, followed by descriptors such as “(enantiomer 1)”, “(stereoisomer 2)”, and the like. Likewise, example compounds obtained from starting materials being single stereoisomers of unknown absolute configuration are disclosed herein in an analogous fashion. The order of the full chemical names (including (R) and (S) configurations) cannot be construed as to encode for any correlation to the individual intermediate or example numbers.

Preparative Flash Chromatography

Instrument: Biotage Isolera Four; pump: Dual-Piston; flow rate: 1 to 200 mL/min; internal detector: 200-400 nm (variable UV detector); solvent inlets: 4; cartridges: Biotage SNAP Ultra™, sizes: 10 g, 25 g, 50 g, 100 g, 340 g, media: Biotage® HP-Sphere—25 micron spherical silica, resolution: minimum 7000 N/m (plates per meter) typical 10,000 N/m; solvent A: hexane, solvent B: ethyl acetate, solvent C: dichlormethane, solvent D: ethanol; solvent E: methanol; UV collection modes: single/dual/A-All wavelengths (variable UV); fractionation modes: volume, threshold, threshold with volume, low slope, medium slope, custom slope or via external detection

Method X: gradient with solvent A and B, λ-all

Method Y: gradient with solvent C and D, λ-all

Method Z: gradient with solvent C and E, λ-all

The various aspects of the invention described in this application are illustrated by the following examples which are not meant to limit the invention in any way.

The example testing experiments described herein serve to illustrate the present invention and the invention is not limited to the examples given.

EXPERIMENTAL SECTION—PREPARATION OF INTERMEDIATES Intermediate 1 1-(4-{[tert-butyl(dimethyl)silyl]oxy}phenyl)ethan-1-one

1-(4-Hydroxyphenyl)ethan-1-one (2.00 g, 14.7 mmol) was provided in dichloromethane (25 mL) at 0° C. 4-dimethylaminopyridine (100 mg, 819 μmol; CAS-RN 1122-58-3) and imidazole (2.00 g, 29.4 mmol; CAS-RN 288-32-4) were added. To the mixture was added dropwise a solution of tert-butyl(chloro)dimethylsilane (3.32 g, 22.0 mmol) dichloromethane. The reaction mixture was stirred for 2 h at rt. The mixture was poured into aqueous sodiumbicarbonate solution and extracted with dichloromethane. The organic layer was washed with water, dried and concentrated under reduced pressure. The residue was purified by flash chromatography (silica gel, hexane/ethyl acetate gradient 0-10%) to give 3.7 g (96% yield) of the title compound.

¹H-NMR (400 MHz, CHLOROFORM-d): δ ppm=0.23 (s, 6H), 0.99 (s, 9H), 2.55 (s, 3H), 6.85-6.89 (m, 2H), 7.86-7.90 (m, 2H)

Intermediate 2 tert-butyl(dimethyl)(4-{1-[(trimethylsilyl)oxy]ethenyl}phenoxy)silane

Lithium diisopropylamide solution in tetrahydrofurane (1.5 mL, 2.0 M, 3.0 mmol; CAS-RN 4111-54-0) was provided under argon at −70° C., and 1-(4-{[tert-butyl(dimethyl)silyl]oxy}phenyl)ethan-1-one (500 mg, 2.00 mmol, Intermediate 1) was added. The mixture was allowed to warm to 0° C. and was stirred for 30 min at 0° C. Chlorotrimethylsilane (1.6 mL, 13 mmol; CAS-RN 75-77-4) was added and the reaction mixture was stirred for 4 h at rt. The mixture was poured into water/brine and extracted with methyl tert-butylether. The combined organic layer was washed with brine, dried and concentrated under reduced pressure to give 700 mg (98% yield) of the title compound as a yellow oil.

¹H-NMR (400 MHz, CHLOROFORM-d): δ ppm=0.07 (s, 9H), 0.77-0.82 (m, 15H), 4.13-4.15 (m, 1H), 4.60 (d, J=1.77 Hz, 1H), 6.57-6.61 (m, 2H), 7.24-7.29 (m, 2H).

Intermediate 3 2-bromo-1-(4-{[tert-butyl(dimethyl)silyl]oxy}phenyl)ethan-1-one

Tert-butyl(dimethyl)(4-{1-[(trimethylsilyl)oxy]ethenyl}phenoxy)silane (695 mg, 2.15 mmol; Intermediate 2) was provided at 0° C. in tetrahydrofurane (57 mL) and N-bromosuccinimide (460 mg, 2.59 mmol; CAS-RN 128-08-5) was added. The reaction mixture was stirred overnight at rt. The mixture was treated with water/brine and extracted with ethyl acetate. The combined organic layer was washed with brine, dried and concentrated under reduced pressure. The residue was purified by flash chromatography (silica, hexane/ethyl acetate gradient 0-10%) to give 460 mg (62% yield) of the title compound.

¹H-NMR (400 MHz, CHLOROFORM-d): 5 ppm=0.24-0.26 (m, 6H), 0.99 (s, 9H), 4.40 (s, 2H), 6.90 (d, J=8.87 Hz, 2H), 7.91 (d, J=8.87 Hz, 2H).

LC-MS (method 2): R_(t)=1.58 min; MS (ESIpos): m/z=329.2 [M+H]⁺

Intermediate 4 [4-amino-2-(4-methoxy-2-methyl-anilino)thiazol-5-yl]-phenyl-methanone

1-Isothiocyanato-4-methoxy-2-methylbenzene (225.1 mg, 1.26 mmol) was dissolved in acetonitrile (25 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (190 mg, 1.26 mmol) and cyanamide (63.4 mg, 1.51 mmol). After stirring for 45 min at rt further 1,8-diazabicyclo(5.4.0)undec-7-ene (96 mg, 0.62 mmol) and 2-bromo-1-phenylethanone (225 mg, 1.26 mmol) were added. The reaction mixture was stirred at rt overnight. The suspension was treated with water and the precipitate was isolated by filtration, washed with water and some ethyl acetate and dried by lyophilization to give 351 mg (78% yield) of the title compound.

¹H-NMR: (400 MHz, DMSO-d6): δ ppm=2.19 (s, 3H), 3.74 (s, 3H), 6.78 (dd, J=8.62, 2.79 Hz, 1H), 6.88 (d, J=2.79 Hz, 1H), 7.26 (d, J=8.62 Hz, 1H), 7.34-7.47 (m, 3H), 7.50-7.62 (m, 2H), 7.75-8.52 (m, 2H), 9.90-10.16 (br s, 1H).

LC-MS (method 2) Rt=1.09 min; MS (ESIpos): m/z=340.1 [M+H]⁺

The following intermediates were prepared from commercial starting materials stated in Table 2, below, using the procedure as for Intermediate 4, followed by purification by chromatography if needed. If no purification is specified, the respective title compound was isolated as crude product.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) if necessary. In case of a missing precipitation, the reaction mixture was extracted three times with ethyl acetate. The combined organic phases were dried over sodium sulfate, filtrated and evaporated to dryness. The crude product was purified by chromatography as stated in Table 2.

TABLE 2 Intermedediates 5-77 Intermediate Chemical structure Starting number Compound name materials Analytics/purification/yield  5

4- isothiocyanato- N,N- dimethylaniline; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.87 (s, 6 H), 6.72 (d, J = 9.13 Hz, 2 H), 7.30 (br d, J = 8.36 Hz, 2 H), 7.38- 7.51 (m, 3 H), 7.61 (dd, J = 7.48, 2.15 Hz, 2 H), 7.82- 8.55 (m, 2 H), 10.32-10.58 (brs, 1 H). LC-MS (method 1) Rt = 1.00 min; MS (ESIpos): m/z = 339.3 [M + H]⁺ RP-HPLC (method C, acidic) 10% yield  6

1-isopropoxy-4- isothiocyanato- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.24 (d, J = 6.08 Hz, 6 H), 4.56 (spt, J = 6.04 Hz, 1 H), 6.88-6.95 (m, 2 H), 7.42-7.49 (m, 5 H), 7.61-7.67 (m, 2 H), 7.89-8.41 (m, 2 H), 10.57-10.61 (br s, 1 H). LC-MS (method 1) Rt = 1.24 min; MS (ESIpos): m/z = 354.8 [M + H]⁺ RP-HPLC (method D, acidic) 66% yield  7

1,3,5- trifluoro-2- isothiocyanato- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.38 (m, 2 H), 7.43-7.52 (m, 3 H), 7.63 (m, 2 H), 7.97-8.42 (m, 2 H), 10.22-10.38 (br s, 1 H). LC-MS (method 2) Rt = 0.81 min; MS (ESIpos): m/z = 350.5 [M + H]⁺ RP-HPLC (method C, basic) 68% yield  8

2-bromo-4- fluoro-1- isothiocyanato- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.33 (td, J = 8.55, 2.91 Hz, 1 H), 7.39-7.51 (m, 3 H), 7.61 (dd, J = 7.48, 1.90 Hz, 2 H), 7.66-7.75 (m, 2 H), 7.96-8.45 (m, 2 H), 10.20-10.49 (br s, 1 H). LC-MS (method 2) Rt = 0.81 min; MS (ESIpos): m/z = 350.5 [M + H]⁺ RP-HPLC (method C, basic) 33% yield  9

1-fluoro-4- isothiocyanato- benzene; 2-chloro-1-(6- methylpyridin- 3-yl)ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.52 (s, 3 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.36 (d, J = 7.86 Hz, 1 H), 7.60-7.67 (m, 2 H), 7.92 (dd, J = 7.86, 2.28 Hz, 1 H), 8.14-8.48 (m, 2 H), 8.72 (d, J = 2.03 Hz, 1 H), 10.87 (s, 1 H). LC-MS (method 2) Rt = 0.86 min; MS (ESIpos): m/z = 329.2 [M + H]⁺ 72% yield 10

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1- (4-pyridyl) ethanone; hydrobromide (1:1) ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.20-7.26 (m, 2 H), 7.54-7.59 (m, 1 H),7.56- 7.58 (m, 1 H), 7.60-7.67 (m, 2 H), 8.27-8.47 (m, 2 H), 8.70 (d, J = 5.83 Hz, 2 H), 10.83- 11.02 (br s, 1 H). LC-MS (method 2) Rt = 0.77 min; MS (ESIpos): m/z = 315.1 [M + H]⁺ 76% yield 11

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(2- fluorophenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21 (t, J = 8.87 Hz, 2 H), 7.24-7.31 (m, 2 H), 7.44-7.53 (m, 2 H), 7.55- 7.63 (m, 2 H), 8.16 (br s, 2 H), 10.79 (s, 1 H). LC-MS (method 2) Rt = 0.97 min; MS (ESIpos): m/z = 332.3 [M + H]⁺ 92% yield 12

4- isothiocyanato- benzonitrile; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.45-7.52 (m, 3 H), 7.68 (dd, J = 7.73, 1.65 Hz, 2 H), 7.81 (s, 4 H), 8.17-8.40 (m, 2 H), 11.08-11.35 (br s, 1 H). LC-MS (method 2) Rt = 0.92 min; MS (ESIpos): m/z = 321.1 [M + H]⁺ 56% yield 13

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1- [4-chloro-3- (trifluoromethyl) phenyl]ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.66 (br d, J = 4.06 Hz, 2 H), 7.87 (s, 1 H), 7.95- 8.01 (m, 1 H), 8.07 (s, 1 H), 8.28-8.49 (m, 2 H), 10.90- 10.96 (br s, 1 H). LC-MS (method 2) Rt = 1.26 min; MS (ESIpos): m/z = 416.2 [M + H]⁺ RP-HPLC (method D, basic) 31% yield 14

2-chloro-4- fluoro-1- isothiocyanato- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.25-7.31 (m, 1 H), 7.41-7.50 (m, 3 H), 7.58 (dd, J = 8.49, 2.91 Hz, 1 H), 7.60-7.66 (m, 2 H), 7.81 (dd, J = 9.12, 5.83 Hz, 1 H), 8.17 (br s, 2 H), 10.36 (br s, 1 H). LC-MS (method 2) Rt = 1.00 min; MS (ESIpos): m/z = 348.5 [M + H]⁺ RP-HPLC (method C, basic) 67% yield 15

1-fluoro-4- isothiocyanato- benzene; 3-(bromoacetyl) benzonitrile ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.61-7.66 (m, 2 H), 7.69 (t, J = 7.98 Hz, 1 H), 7.97 (dd, J = 7.86, 1.77 Hz, 2 H), 8.06 (t, J = 1.52 Hz, 1 H), 8.33 (br s, 2 H), 10.81-10.97 (m, 1 H). LC-MS (method 2) Rt = 0.97 min; MS (ESIpos): m/z = 339.2 [M + H]⁺ 78% yield 16

2-chloro-4- isothiocyanato- N,N- dimethylaniline; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.68 (s, 6 H), 7.14 (d, J = 8.87 Hz, 1 H), 7.34 (dd, J = 8.62, 2.53 Hz, 1 H), 7.42-7.50 (m, 3 H), 7.60- 7.69 (m, 2 H), 7.69-7.74 (m, 1 H), 8.22 (br s, 2 H), 10.58 (br s, 1 H). LC-MS (method 2) Rt = 1.14 min; MS (ESIpos): m/z = 373.2 [M + H]⁺ RP-HPLC (method D, basic) 66% yield 17

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(6- methoxypyridin- 3-yl)ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.91 (s, 3 H), 6.91 (dd, J = 8.49, 0.63 Hz, 1 H), 7.19-7.26 (m, 2 H), 7.62- 7.66 (m, 2 H), 7.98 (dd, J = 8.62, 2.53 Hz, 1 H), 8.24 (br s, 2 H), 8.51 (dd, J = 2.53, 0.76 Hz, 1 H), 10.85 (br s, 1 H). LC-MS (method 1) Rt = 1.09 min; MS (ESIpos): m/z = 345.2 [M + H]⁺ 72% yield 18

1-fluoro-4- isothiocyanato- benzene; 4- (bromoacetyl)- N-methyl- benzamide LC-MS (method 2) Rt = 0.79 min; MS (ESIpos): m/z = 371.2 [M + H]⁺ RP-HPLC (method C, basic) 31% yield 19

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(3- fluorophenyl) ethanone LC-MS (method 2) Rt = 1.03 min; MS (ESIpos): m/z = 332.2 [M + H]⁺ RP-HPLC (method D, basic) 13% yield 20

2-chloro-4- isothiocyanato- 1-methoxy- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.83 (s, 3 H), 7.15 (d, J = 8.87 Hz, 1 H), 7.41 (dd, J = 8.87, 2.79 Hz, 1 H), 7.44-7.51 (m, 3 H), 7.62- 7.69 (m, 2 H), 7.82 (d, J = 2.03 Hz, 1 H), 8.27 (br s, 2 H), 10.71 (s, 1 H). LC-MS (method 2) Rt = 0.98 min; MS (ESIpos): m/z = 360.2 [M + H]⁺ 39% yield 21

1- isothiocyanato- 4-methoxy- benzene; 2-bromo-1-(4- fluorophenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.74 (s, 3 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.28 (t, J = 8.87 Hz, 2 H), 7.45 (br d, J = 8.62 Hz, 2 H), 7.67-7.73 (m, 2 H), 7.89-8.50 (m, 2 H), 10.64 (br s, 1 H). LC-MS (method 2) Rt = 1.09 min; MS (ESIpos): m/z = 344.5 [M + H]⁺ preparative flash chromatography (method Y, 1-10%) 66% yield 22

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-[4- (methylsulfonyl) phenyl]ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.28 (s, 3 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.60- 7.65 (m, 2 H), 7.88 (d, J = 8.62 Hz, 2 H), 8.00-8.04 (m, 2 H), 8.31 (br s, 2H), 9.20 (s, 1 H). LC-MS (method 2) Rt = 0.83 min; MS (ESIpos): m/z = 392.2 [M + H]⁺ 92% yield 23

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1- [4-(1H- imidazol-1-yl) phenyl]ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.14-7.15 (m, 1 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.58-7.69 (m, 2 H), 7.75- 7.82 (m, 4 H), 7.84 (t, J = 1.39 Hz, 1 H), 8.00-8.56 (m, 2 H), 8.37 (t, J = 1.14 Hz, 1 H), 10.84 (br s, 1 H). LC-MS (method 2) Rt = 0.88 min; MS (ESIpos): m/z = 380.2 [M + H]⁺ RP-HPLC (method C, basic) 16% yield 24

1-fluoro-4- isothiocyanato- benzene; 4- (bromoacetyl)- 2-fluorobenzo- nitrile ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.58-7.63 (m, 2H), 7.65 (dd, J = 7.98, 1.39 Hz, 1 H), 7.72 (dd, J = 9.76, 1.39 Hz, 1 H), 8.04 (dd, J = 7.86, 6.59 Hz, 1 H), 8.39 (br s, 2 H), 10.92 (br s, 1 H). LC-MS (method 2) Rt = 0.92 min; MS (ESIpos): m/z = 357.2 [M + H]⁺ RP-HPLC (method C, basic) 5% yield 25

1-fluoro-4- isothiocyanato- benzene; 4- (bromoacetyl)- 3-fluorobenzo- nitrile ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.23 (t, J = 8.87 Hz, 2 H), 7.60-7.67 (m, 3 H), 7.73 (dd, J = 9.89, 1.27 Hz, 1 H), 8.05 (dd, J = 7.86, 6.59 Hz, 1 H), 8.41 (br s, 2 H), 10.94 (br s, 1 H). LC-MS (method 2) Rt = 0.92 min; MS (ESIpos): m/z = 357.2 [M + H]⁺ RP-HPLC (method D, acidic) 13% yield 26

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1- (2-fluoro-4- methoxyphenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.81 (s, 3 H), 6.83 (dd, J = 8.49, 2.41 Hz, 1 H), 6.89 (dd, J = 12.29, 2.41 Hz, 1 H), 7.21 (t, J = 8.87 Hz, 2 H), 7.42 (t, J = 8.49 Hz, 1 H), 7.56- 7.63 (m, 2 H), 7.99-8.17 (br s, 2 H), 10.77 (br s, 1 H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 362.4 [M + H]⁺ 81% yield 27

1- isothiocyanato- 4-(trifluoro- methoxy) benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.38 (d, J = 8.36 Hz, 2 H), 7.45-7.52 (m, 3 H), 7.67 (dd, J = 7.60, 1.77 Hz, 2 H), 7.74 (d, J = 9.13 Hz, 2 H), 8.03- 8.37 (br s, 2 H), 10.94 (br s, 1 H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 380.5 [M + H]⁺ preparative flash chromatography (method X, 25-90%) 21% yield 28

1-fluoro-4- isothiocyanato- benzene; 2-chloro-1-(3,4- difluorophenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.52-7.58 (m, 2 H), 7.61-7.73 (m, 3 H), 8.06- 8.52 (br s, 2 H), 10.87 (br s, 1 H). LC-MS (method 2) Rt = 1.13 min; MS (ESIpos): m/z = 350.2 [M + H]⁺ RP-HPLC (method D, basic) 34% yield 29

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(3,4- dichlorophenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.19 (t, J = 8.87 Hz, 2H), 7.56 (br s, 2 H), 7.63 (dd, J = 8.36, 2.03 Hz, 1 H), 7.73 (d, J = 8.36 Hz, 1 H), 7.84 (d, J = 1.77 Hz, 1 H), 8.03-8.58 (m, 2 H), 10.83 (br s, 1 H). LC-MS (method 2) Rt = 1.22 min; MS (ESIneg): m/z = 380.3 [M - H]⁺ RP-HPLC (method D, basic) 32% yield 30

1- isothiocyanato- 4-(trifluoro- methoxy) benzene; 2-bromo-1-(4- fluorophenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.31 (t, J = 8.87 Hz, 2 H), 7.37 (d, J = 8.36 Hz, 2 H), 7.70-7.77 (m, 4 H), 8.07- 8.34 (br s, 2 H), 10.98 (br s, 1 H). LC-MS (method 2) Rt = 1.20 min; MS (ESIpos): m/z = 398.2 [M + H]⁺ 89% yield 31

1- isothiocyanato- 4-(trifluoro- methoxy) benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.45-7.52 (m, 3 H), 7.66-7.73 (m, 4 H), 7.83 (br d, J = 8.36 Hz, 2 H), 8.24 (br s, 2 H), 11.13 (br s, 1 H). LC-MS (method 2) Rt = 1.14 min: MS (ESIpos): m/z = 364.2 [M + H]⁺ 85% yield 32

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-[6- (trifluoromethyl) pyridin-3-yl] ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.55-7.64 (m, 2 H), 8.02 (d, J = 7.86 Hz, 1 H), 8.39 (br s, 1 H), 8.29 (dd, J = 7.86, 1.77 Hz, 2 H), 8.99 (d, J = 1.77 Hz, 1 H), 10.95 (br s, 1 H). LC-MS (method 2) Rt = 1.03 mm: MS (ESIpos): m/z = 383.1 [M + H]⁺ RP-HPLC (method C, basic) 97% yield 33

4-chloro-2- fluoro-1- isothiocyanato- benzene; 2-bromo-1- phenylethanone LC-MS (method 2) Rt = 0.97 min; MS (ESIpos): m/z = 348.2 [M + H]⁺ 94% yield 34

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(2,3- dihydro-1H- inden-5-yl) ethanone LC-MS (method 2) Rt = 1.25 min; MS (ESIpos): m/z = 354.5 [M + H]⁺ 89% yield 35

1,2- difluoro-4- isothiocyanato- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21-7.28 (m, 1 H), 7.38-7.54 (m, 4 H), 7.63- 7.72 (m, 2 H), 7.90-8.02 (m, 1 H), 8.10-8.46 (br s, 2 H), 10.86-11.07 (br s, 1 H). LC-MS (method 2) Rt = 1.05 min; MS (ESIpos): m/z = 332.2 [M + H]⁺ 76% yield 36

1-fluoro-4- isothiocyanato- benzene; 4- (bromoacetyl) benzonitrile ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.62 (br dd, J = 8.87, 4.82 Hz, 2 H), 7.80 (d, J = 8.36 Hz, 2 H), 7.95 (d, J = 1.00 Hz, 2 H), 8.32 (br s, 2 H), 10.84-10.95 (br s, 1 H). LC-MS (method 2) Rt = 0.97 min; MS (ESIpos): m/z = 339.2 [M + H]⁺ 84% yield 37

1-chloro-4- isothiocyanato- benzene; 2-bromo-1- phenylethanone LC-MS (method 1) Rt = 1.23 min; MS (ESIneg): m/z = 328.3 [M - H]⁺ 100% yield 38

1,2-dichloro-4- isothiocyanato- benzene; 2-bromo-1- phenylethanone LC-MS (method 1) Rt = 1.32 min; MS (ESIneg); m/z = 362.3 [M - H]⁺ preparative flash chromatography (method X, 10-80%) 65% yield 39

1-chloro-2- fluoro-4- isothiocyanato- benzene; 2-bromo-1- phenylethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.28 (br dt, J = 8.87, 1.27 Hz, 1 H), 7.45- 7.57 (m, 4 H), 7.68 (dd, J = 7.60, 1.77 Hz, 2H), 7.99 (dd, J = 11.91, 2.03 Hz, 1 H), 8.27 (br s, 2 H), 11.07 (br s, 1 H). LC-MS (method 2) Rt = 1.04 min; MS (ESIpos): m/z = 348.1 [M + H]⁺ 67% yield 40

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(4- chlorophenyl) ethanone LC-MS (method 2) Rt = 1.12 min; MS (ESIpos); m/z = 348.1 [M + H]⁺ 94% yield 41

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(4- chlorophenyl) ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21 (t, J = 8.87 Hz, 2 H), 7.54 (d, J = 8.62 Hz, 2 H), 7.62 (br dd, J = 8.87, 5.07 Hz, 2 H), 7.68 (d, J = 8.62 Hz, 2 H), 8.28 (br s, 2 H), 10.83 (br s, 1 H). LC-MS (method 1) Rt = 1.25 min; MS (ESIneg): m/z = 346.3 [M - H]⁺ preparative flash chromatography (method Y, 0-15%) 53% yield 42

1-fluoro-4- isothiocyanato- benzene; ethyl 2-[4- (bromoacetyl) phenoxy]-2- methyl- propanoate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.15 (t, J = 7.10 Hz, 3H), 1.56 (s, 6 H), 4.17 (q, J = 7.10 Hz, 2 H), 6.78 (d, J = 8.87 Hz, 2 H), 7.12 (t, J = 8.87 Hz, 2 H), 7.34-7.51 (br s, 2 H), 7.58 (d, J = 8.62 Hz, 2 H), 7.62-8.80 (m, 2 H), 9.78- 10.70 (m, 1 H). LC-MS (method 2) Rt = 1.25 min; MS (ESIpos): m/z = 444.3 [M + H]⁺ 93% yield 43

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-[4- (trifluorome- thoxy)phenyl] ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21 (t, J = 8.87 Hz, 2 H), 7.46 (dd, J = 8.74, 0.89 Hz, 2 H), 7.55-7.66 (m, 2 H), 7.79 (d, J = 8.87 Hz, 2 H), 8.36 (br s, 2H), 10.77 (br s, 1 H). LC-MS (method 2) Rt = 1.21 min; MS (ESIpos): m/z = 398.1 [M + H]⁺ RP-HPLC (method D, basic) 50% yield 44

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1- (4-chlorophenyl) ethanone LC-MS (method 2) Rt = 1.19 min; MS (ESIpos): m/z = 364.1 [M + H]⁺ 87% yield 45

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1- [4- (trifluoromethyl) phenyl]ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22 (t, J = 8.87 Hz, 2 H), 7.61-7.65 (m. 2 H), 7.85 (s, 4 H), 8.36 (br s, 2 H), 10.88 (br s, 1 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 382.2 [M + H]⁺ 66% yield 46

1- isothiocyanato- 4- (trifluoromethyl) benzene; 2-bromo-1-[4- (difluoro- methoxy) phenyl] ethanone LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 430.2 [M + H]⁺ 87% yield 47

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-[4- (difluoro- methoxy) phenyl] ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.34 (t, J = 74.50 Hz, 1 H), 7.17-7.31 (m, 4 H), 7.61 (br dd, J = 8.62, 4.82 Hz, 2 H), 7.73 (d, J = 8.62 Hz, 2 H), 8.23 (br s, 2 H), 10.82 (br s, 1 H). LC-MS (method 2) Rt = 1.11 min; MS (ESIpos): m/z = 380.4 [M + H]⁺ RP-HPLC (method D, basic) 94% yield 48

phenyl isothiocyanate; 2-bromo-4′- hydroxyace- tophenone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 6.78 (d, 2 H). 7.04 (t, 1 H), 7.33 (d, 2H), 7.54 (d, 2 H), 7.57 (d, 2 H), 8.06 (br s, 2 H), 9.95 (br s, 1 H), 10.68 (br s, 1 H) LC-MS (method 2) Rt = 0.61 min; MS (ESIpos): m/z = 312 [M + H]⁺ preparative flash chromatography (ethyl acetate/heptane 4/1) 80% yield 49

4-chloro-2- fluoro-1- isothiocyanato- benzene; 2-bromo-1-[4- (difluorome- thoxy)phenyl] ethanone LC-MS (method 2) Rt = 1.00 min; MS (ESIpos): m/z = 414.2 [M + H]⁺ quantitave yield 50

1-chloro-4- isothiocyanato- benzene; 2-bromo-1-[4- (difluoro- methoxy) phenyl] ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.35 (t, J = 74.77 Hz, 1 H), 7.26 (d, J = 8.62 Hz, 2 H), 7.42 (d, J = 8.87 Hz, 1 H), 7.66 (d, J = 8.87 Hz, 2 H), 7.75 (d, J = 8.62 Hz, 2 H), 8.26 (br s, 2 H), 10.92 (br s, 1 H). LC-MS (method 2) Rt = 1.19 min; MS (ESIpos): m/z = 396.2 [M + H]⁺ RP-HPLC (method D, acidic) quantitative yield 51

1-chloro-2- fluoro-4- isothiocyanato- benzene; 2-bromo-1-[4- (difluoro- methoxy) phenyl] ethanone LC-MS (method 2) Rt = 1.08 min; MS (ESIpos): m/z = 414.2 [M + H]⁺ 95% yield 52

1,2- difluoro-4- isothiocyanato- benzene; 2-bromo-1-[4- (difluoro- methoxy) phenyl] ethanone ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.35 (t, J = 76.04 Hz, 1 H), 7.27 (br d, J = 8.62 Hz, 3H), 7.39-7.48 (m, 1 H), 7.75 (d, J = 8.87 Hz, 2 H), 7.97 (ddd, J = 12.99, 7.29, 2.28 Hz, 1 H), 8.27 (br s, 2 H), 10.99 (br s, 1 H). LC-MS (method 2) Rt = 1.06 min; MS (ESIpos): m/z = 398.2 [M + H]⁺ 87% yield 53

2-bromo-1- (pyridin-4- yl)ethan-1- one salt with hydrogen bromide; 1,2- difluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.22-7.28 (m, 1 H), 7.39-7.47 (m, 1 H), 7.56- 7.59 (m, 2 H), 7.91-7.99 (m, 1 H), 8.39 (td, J = 6.46, 2.79 Hz, 1 H), 8.36-8.44 (m, 1 H), 8.68- 8.74 (m, 2 H), 10.78-11.25 (m, 1 H). LC-MS (method 1) Rt = 0.89 min; MS (ESIpos): m/z = 333.1 [M + H]⁺ RP-HPLC (method C, acidic) 76% yield 54

2-bromo-1-(4- methoxyphenyl) ethan-1-one; 1,2- difluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.82 (s, 3 H), 7.02 (d, J = 8.87 Hz, 2 H), 7.22- 7.28 (m, 1 H), 7.38-7.47 (m, 1 H), 7.68 (d, J = 8.87 Hz, 2 H), 7.91-8.03 (m, 1 H), 8.19 (br s, 2 H), 10.81-11.06 (m, 1 H). LC-MS (method 2) Rt = 1.07 min; MS (ESIpos): m/z = 362.1 [M + H]⁺ 92% yield 55

2-bromo-1-(6- methoxypyridin- 3-yl)ethan- 1-one; 1,2-difluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.54 (s, 3 H), 6.92 (dd, J = 8.62, 0.51 Hz, 1 H), 7.28 (br s, 1 H), 7.40-7.49 (m, 1 H), 7.94-8.03 (m, 2 H), 8.18-8.36 (m, 2 H), 8.53 (dd, J = 2.53, 0.76 Hz, 1 H), 10.98- 11.04 (m, 1 H). LC-MS (method 2) Rt = 0.92 min; MS (ESIpos): m/z = 363.2 [M + H]⁺ 77% yield 56

2-bromo-1-[6- (trifluoro- methyl)pyridin- 3-yl]ethan- 1-one; 1,2-difluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21-7.27 (m, 1 H), 7.38-7.47 (m, 1 H),7.87- 7.98 (m, 1 H), 8.03 (d, J = 8.36 Hz, 1 H), 8.31 (dd, J = 8.11, 2.03 Hz, 1 H), 8.33-8.52 (m, 2 H), 9.00 (d, J = 1.52 Hz, 1 H), 11.04-11.24 (m, 1 H). LC-MS (method 2) Rt = 0.96 min; MS (ESIpos): m/z = 401.3 [M + H]⁺ 74% yield 57

2-bromo-1- (pyridin-4- yl)ethan-1- one salt with hydrogen bromide; 1-chloro-2- fluoro-4- isothiocyanato- benzene LC-MS (method 2) Rt = 0.77 min: MS (ESIpos): m/z = 349.1 [M + H]⁺ 69% yield 58

2-bromo-1- (pyridin-4- yl)ethan-1- one salt with hydrogen bromide; 1-chloro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.78 (d, J = 12.42 Hz, 1 H), 4.08 (d, J = 12.17 Hz, 1 H), 7.18-7.23 (m, 2 H), 7.31- 7.36 (m, 2 H), 7.56-7.60 (m, 2 H), 8.39 (s, 1 H), 8.48-8.55 (m, 2 H). LC-MS (method 2) Rt = 0.84 min; MS (ESIpos): m/z = 331.1 [M + H]⁺ 57% yield 59

2-bromo-1- (4- methoxyphenyl) ethan-1-one; 1-chloro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.82 (s, 3 H), 7.01 (d, J = 8.87 Hz, 2 H), 7.38- 7.43 (m, 2 H), 7.61-7.70 (m, 4 H), 8.03-8.27 (m, 2 H), 10.78- 10.92 (m, 1 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 360.1 [M + H]⁺ 94% yield 60

2-bromo-1-(4- methoxyphenyl) ethan-1-one; 1-chloro-2- fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.82 (s, 3 H), 6.99-7.03 (m, 2 H), 7.25 (dd, J = 8.74, 1.65 Hz, 1 H), 7.51 (t, J = 8.74 Hz, 1 H), 7.68 (d, J = 8.87 Hz, 2 H), 7.95 (br d, J = 12.17 Hz, 1 H), 8.06-8.29 (m, 2 H), 10.92-11.16 (m, 1 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 378.1 [M + H]⁺ 80% yield 61

2-bromo-1- phenylethan- 1-one; isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.08 (t, J = 7.60 Hz, 1 H), 7.36 (t, J = 7.86 Hz, 2 H), 7.44-7.51 (m, 3 H), 7.61 (d, J = 7.86 Hz, 2 H), 7.64-7.69 (m, 2 H), 8.21 (br s, 2 H), 10.78 (br s, 1 H). LC-MS (method 2) Rt = 1.05 min; MS (ESIpos): m/z = 296.4 [M + H]⁺ 14% yield 62

2-bromo-1- phenylethan- 1-one; 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21 (t, J = 8.87 Hz, 2 H), 7.43-7.52 (m, 3 H), 7.59-7.69 (m, 4 H), 8.22 (br s, 2 H), 10.79 (br s, 1 H). LC-MS (method 2) Rt = 1.04 min; MS (ESIpos): m/z = 314.4 [M + H]⁺ preparative flash chromatography (method Z, 0-1%) 78% yield 63

2-bromo-1-(4- methoxyphenyl) ethan-1-one; 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.81 (s, 3 H), 7.01 (d, J = 8.87 Hz, 2 H), 7.21 (t, J = 8.87 Hz, 2 H), 7.66 (d, J = 8.87 Hz, 4H), 8.15 (br s, 2 H), 10.77 (br s, 1 H). LC-MS (method 2) Rt = 1.09 min; MS (ESIpos): m/z = 344.4 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 57% yield 64

2-bromo-1-(4- methylphenyl) ethan-1-one; 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.35 (s, 3 H), 7.21 (t, J = 8.87 Hz, 2 H), 7.27 (d, J = 7.86 Hz, 2 H), 7.57 (d, J = 8.11 Hz, 2 H), 7.63 (dd, J = 9.13, 4.82 Hz, 2 H), 8.19 (br s, 2H), 10.77 (s, 1 H). LC-MS (method 2) Rt = 1.14 min; MS (ESIpos): m/z = 328.4 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 47% yield 65

2-bromo-1-(4- fluorophenyl) ethan-1-one; 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21 (t, J = 8.87 Hz, 2 H), 7.30 (t, J = 8.87 Hz, 2 H), 7.62 (dd, J = 8.87, 4.82 Hz, 2 H), 7.73 (dd, J = 8.74, 5.45 Hz, 2 H), 8.22 (br s, 2 H), 10.82 (br s, 1 H). LC-MS (method 2) Rt = 1.05 min; MS (ESIpos): m/z = 332.4 [M + H]⁺ preparative flash chromatography (method Z, 0-2%) 66% yield 66

2-bromo-1- phenylethan- 1-one; 2,4- difluoro-1- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.09-7.17 (m, 1 H), 7.39 (ddd, J = 11.15, 8.74, 2.91 Hz, 1 H), 7.43-7.50 (m, 3 H), 7.62-7.65 (m, 2 H), 8.01 (br td, J = 9.12, 6.34 Hz, 1 H), 8.08-8.39 (m. 2 H), 10.50 (br s, 1 H). LC-MS (method 2) Rt = 0.88 min: MS (ESIpos): m/z = 332.4 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 71% yield 67

2-bromo-1- phenylethan- 1-one; 1- isothiocyanato- 4- methoxybenzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.70-3.76 (m, 3 H), 6.92-6.96 (m, 2 H), 7.42- 7.50 (m, 5 H), 7.60-7.66 (m, 2 H), 7.93-8.48 (m, 2 H), 10.60 (br s, 1 H). LC-MS (method 2) Rt = 1.04 min; MS (ESIpos): m/z = 326.4 [M + H]⁺ preparative flash chromatography (method Z, 0-1%) 76% yield 68

2-bromo-1- (4-{[tert-butyl (dimethyl)silyl] oxy}phenyl) ethan-1-one (Intermediate 3); 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 0.23 (s, 6 H), 0.96 (s, 9 H), 6.92 (d, J = 8.62 Hz, 2 H), 7.21 (t, J = 8.87 Hz, 2 H), 7.59-7.66 (m, 4 H), 8.15 (br s, 2 H), 10.76 (br s, 1 H). LC-MS (method 2) Rt = 1.56 min; MS (ESIpos): m/z = 444.2 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 27% yield 69

2-bromo-1- phenylethan- 1-one; 1- isothiocyanato- 4- methylbenzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.27 (s, 3 H), 7.16 (d, J = 8.11 Hz, 2 H), 7.44- 7.49 (m, 5 H), 7.63-7.67 (m, 2 H), 7.93-8.63 (m, 2 H), 10.69 (br s, 1 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 310.5 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 67% yield 70

2-bromo-1- phenylethan- 1-one; 1-fluoro-3- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 6.85-6.93 (m, 1 H), 7.27 (ddd, J = 8.24, 2.03, 0.89 Hz, 1 H), 7.38 (td, J = 8.17, 6.72 Hz, 1 H), 7.45-7.53 (m, 3 H), 7.66-7.70 (m, 2 H), 7.76 (dt, J = 11.66, 2.15 Hz, 1 H), 8.26 (br s, 2 H), 10.96 (s, 1 H). LC-MS (method 2) Rt = 1.07 min; MS (ESIpos): m/z = 314.4 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 53% yield 71

2-bromo-1- phenylethan- 1-one; 1-fluoro-2- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.15-7.25 (m, 2 H), 7.27-7.34 (m, 1 H), 7.43- 7.51 (m, 3 H), 7.62-7.68 (m, 2 H), 8.04-8.11 (m, 1 H), 8.10- 8.32 (m, 2 H), 10.54 (s, 1 H). LC-MS (method 2) Rt = 0.96 min; MS (ESIpos): m/z = 314.5 [M + H]⁺ preparative flash chromatography (method Z, 0-1%) 36% yield 72

2-bromo-1- phenylethan- 1-one; 1- isothiocyanato- 3- methylbenzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.30 (s, 3 H), 6.91 (br d, J = 7.35 Hz, 1 H), 7.24 (br t, J = 7.73 Hz, 1 H), 7.38 (br s, 1 H), 7.41-7.54 (m, 4 H), 7.62-7.68 (m, 2 H), 8.20 (br s, 2 H), 10.70 (br s, 1 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 310.5 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 53% yield 73

2-bromo-1- phenylethan- 1-one; 1- isothiocyanato- 2- methylbenzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 2.23 (s, 3 H), 7.14-7.26 (m,2H), 7.27- 7.31 (m, 1 H), 7.38-7.52 (m, 4 H), 7.57-7.62 (m, 2 H), 8.15 (br s, 2 H), 10.18 (br s, 1 H). LC-MS (method 2) Rt = 1.10 min; MS (ESIpos): m/z = 310.5 [M + H]⁺ preparative flash chromatography (method Z, 0-1%) 60% yield 74

2-bromo-1- phenylethan- 1-one; 4- isothiocyanato- 1-methyl-1H- pyrazole ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 3.81 (s, 3 H), 7.41-7.52 (m, 4 H), 7.58- 7.66 (m, 2 H), 7.90 (s, 1 H), 7.94-8.50 (m, 2 H), 10.44- 10.57 (m, 1 H). LC-MS (method 2) Rt = 0.78 min; MS (ESIpos): m/z = 300.5 [M + H]⁺ preparative flash chromatography (method Z, 0-1%) 58% yield 75

2-bromo-1- phenylethan- 1-one; 3- isothiocyanato- pyridine ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.37-7.42 (m, 1 H), 7.45-7.53 (m, 3 H), 7.65- 7.69 (m, 2 H), 8.12 (br ddd, J = 8.36, 2.53, 1.52 Hz, 1 H), 8.16-8.39 (m, 2 H), 8.28 (dd, J = 4.69, 1.39 Hz, 1 H), 8.81 (d, J = 2.53 Hz, 1 H), 10.95 (br s, 1 H). LC-MS (method 2) Rt = 0.70 min; MS (ESIpos): m/z = 297.4 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 23% yield 76

2-bromo-1-(4- bromophenyl) ethan-1-one; 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 7.21 (t, J = 8.87 Hz, 2 H), 7.56-7.65 (m, 4 H), 7.66-7.70 (m, 2 H), 8.26 (br s, 2 H), 10.85 (br s, 1 H). LC-MS (method 2) Rt = 1.19 min; MS (ESIpos): m/z = 392.3 [M + H]⁺ preparative flash chromatography (method Z, 0-1%) 77% yield 77

1-[4-(benzyloxy) phenyl]-2- bromoethan- 1-one; 1-fluoro-4- isothiocyanato- benzene ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 5.17 (s, 2 H), 7.09 (d, J = 8.87 Hz, 2 H), 7.21 (t, J = 8.87 Hz, 2 H), 7.31-7.37 (m, 1 H), 7.41 (t, J = 7.35 Hz, 2 H), 7.45-7.49 (m, 2 H), 7.60- 7.69 (m, 4 H), 7.96-8.34 (m, 2 H), 10.71-10.79 (m. 1 H). LC-MS (method 2) Rt = 1.30 min; MS (ESIpos): m/z = 420.2 [M + H]⁺ 87% yield

Intermediate 78 [4-(difluoromethoxy)phenyl][2-(4-fluoroanilino)-4-methyl-1,3-thiazol-5-yl]methanone

1-Fluoro-4-isothiocyanatobenzene (116 mg, 0.755 mmol) was dissolved in acetonitrile (7.5 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (100 mg, 0.66 m mol) and ethanimidamide (salt with hydrogen chloride) (86 mg, 0.91 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (70 mg, 0.47 mmol) and 2-bromo-1-[4-(difluoromethoxy)phenyl]ethan-1-one (200 mg, 0.755 mmol) were added. The reaction mixture was stirred at rt overnight. The reaction mixture was concentrated under vacuum and the residue was purified by preparative HPLC (method D, basic) to give 72 mg (25% yield) of the title compound.

LC-MS (method 2) Rt=1.30 min; MS (ESIpos): m/z=379.4 [M+H]⁺

Intermediate 79 [2-(3,4-difluoroanilino)-4-methyl-1,3-thiazol-5-yl][4-(difluoromethoxy)phenyl]methanone

1,2-difluoro-4-isothiocyanatobenzene (129 mg, 0.755 mmol) was dissolved in acetonitrile (7.5 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (100 mg, 0.66 mmol) and ethanimidamide (salt with hydrogen chloride) (86 mg, 0.91 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (70 mg, 0.47 mmol) and 2-bromo-1-[4-(difluoromethoxy)phenyl]ethan-1-one (200 mg, 0.755 mmol) were added. The reaction mixture was stirred at rt overnight. The reaction mixture was concentrated under vacuum and the residue was purified by preparative HPLC (method E, basic) to give 31 mg (10% yield) of the title compound.

LC-MS (method 2) Rt=1.36 min; MS (ESIpos): m/z=397.3 [M+H]⁺

Intermediate 80 Ethyl 2-[4-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]phenoxy]acetate

1-fluoro-4-isothiocyanatobenzene (8.91 g, 58.20 mmol) was dissolved in acetonitrile (450 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (8.86 g, 58.20 mmol) and cyanamide (2.94 g, 69.82 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (4.43 g, 2907 mmol) and ethyl [4-(bromoacetyl)phenoxy]acetate (17.52 g, 58.20 mmol) were added. The reaction mixture was stirred at rt overnight. The suspension was treated with water and the precipitate was isolated by filtration, washed with water and dried by lyophilization to give 18.54 g (77% yield) of the title compound.

¹H-NMR (500 MHz, DMSO-d6): δ ppm=1.22 (t, J=7.09 Hz, 3H), 4.18 (q, J=7.25 Hz, 2H), 4.86 (s, 2H), 7.00 (d, J=9.14 Hz, 2H), 7.21 (t, J=9.14 Hz, 2H), 7.61-7.67 (m, 4H), 8.21 (br s, 2H), 10.77 (s, 1H).

LC-MS (method 2) Rt=1.09 min; MS (ESIpos): m/z=416.3 [M+H]⁺

Intermediate 81 [2-(4-fluoroanilino)-4-methyl-thiazol-5-yl]-phenyl-methanone

1-fluoro-4-isothiocyanatobenzene (77 mg, 0.50 mmol) was solved in acetonitrile (5 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (77 mg, 0.50 mmol) and ethanimidamide (salt with hydrogen chloride) (57 mg, 0.60 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (38 mg, 0.25 mmol) and 2-bromo-1-phenylethanone (100 mg, 0.50 mmol) were added. The reaction mixture was stirred at rt overnight. The solution was filtrated and purified by RP-HPLC (method D, basic). The title compound was isolated by lyophilization (53 mg, 33% yield).

¹H-NMR (400 MHz, DMSO-d6): δ ppm=2.28 (s, 3H), 7.18 (t, J=8.87 Hz, 2H), 7.51 (d, J=7.86 Hz, 2H), 7.53-7.58 (m, 3H), 7.63-7.67 (m, 2H), 10.67 (br s, 1H).

LC-MS (method 2) Rt=1.22 min; MS (ESIpos): m/z=313.1 [M+H]⁺

Intermediate 82 Rac-2-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoic Acid

Rac-ethyl 2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoate (180 mg, 349 μmol, example 39) was dissolved in THF (1.8 ml), cooled to 0° C., and an aqueous solution of sodium hydroxide (350 μl, 1.0 M, 350 μmol) was added. The reaction mixture was stirred overnight at rt. Additional aqueous solution of sodium hydroxide (1.04 mmol) was added and the mixture was stirred for 3 days at rt. The reaction mixture was treated with water, and aqueous solution of hydrochloric acid (1 M) was added to adjust the pH to 5 and afterwards it was extracted three times with dichloromethane. The organic layer was concentrated under reduced pressure to give 36 mg (21% yield) of the title compound.

The aqueous layer was adjusted to pH 3 by addition of aqueous hydrochloric acid (1 M), the formed precipitate was isolated by filtration, washed with water and dried by lyophilization to give 90 mg (53% yield) of the title compound.

¹H NMR (400 MHz, DMSO-d₆): δ ppm=1.15 (d, J=7.35 Hz, 3H), 1.51 (s, 6H), 4.94-5.14 (m, 1H), 6.73-6.79 (m, 2H), 7.22-7.26 (m, 1H), 7.30-7.37 (m, 2H), 7.42-7.47 (m, 2H), 7.58 (br s, 1H), 7.64 (dd, J=8.87, 5.07 Hz, 2H), 7.77-8.44 (m, 2H), 12.81-13.47 (m, 1H)

LC-MS (method 1) Rt=1.00 min; MS (ESIpos): m/z=487.5 [M+H]⁺

Intermediate 83 tert-butyl N-(4-amino-5-benzoyl-thiazol-2-yl)-N-phenyl-carbamate

(4-amino-2-anilino-1,3-thiazol-5-yl)(phenyl)methanone (100 mg, 339 μmol, Intermediate 61) was provided in dichloromethane at rt. N,N-diisopropylethylamine (180 μl, 1.0 mmol; CAS-RN 7087-68-5), 4-(dimethylamino)pyridine (8 mg, 67.7 μmol; CAS-RN 1122-58-3) and di-tert-butyl dicarbonate (81 mg, 372 μmol; CAS-RN 24424-99-5) was added. The reaction mixture was stirred for 3 h at rt. The mixture was diluted with dichloromethane, washed with brine, dried and evaporated to give 120 mg (95% purity, 85% yield) of the title compound.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.32 (s, 9H), 7.35-7.44 (m, 3H), 7.45-7.51 (m, 2H), 7.52-7.58 (m, 3H), 7.69-7.73 (m, 2H), 7.94 (s, 2H).

The following intermediates were prepared from the starting materials stated in Table 3, below, using the procedure as for Intermediate 83.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) after precipitation, extraction or filtration of the reaction mixture if necessary.

TABLE 3 Intermediates 84-96 Intermediate Chemical structure Starting number Compound name materials Analytics/purification/yield 84

Intermediate 62; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 7.31 (t, J = 8.87 Hz, 2 H), 7.44- 7.48 (m, 2 H), 7.50-7.59 (m, 3 H), 7.68-7.74 (m, 2 H), 7.93 (s, 2 H). 91% yield 85

Intermediate 63; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.35 (s, 9 H), 3.84 (s, 3 H), 7.05-7.10 (m, 2 H), 7.31 (t, J = 8.74 Hz, 2 H), 7.46 (dd, J = 8.87, 5.07 Hz, 2 H), 7.73 (d, J = 8.87 Hz, 2 H), 7.86 (s, 2 H). 94% yield 86

Intermediate 64; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, S H), 2.39 (s, 3H), 7.31 (s, 4 H), 7.44 . 7.48 (m, 2 H), 7.61 (s, 2 H), 7.89 (s, 2 H). 79% yield 87

Intermediate 65; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 7.31 (t, J = 8.87 Hz, 2 H), 7.37 (t, J = 9.00 Hz, 2 H), 7.46 (dd, J = 9.12, 5.07 Hz, 2 H), 7.79 (dd, J = 8.87, 5.58 Hz, 2 H), 7.95 (s, 2 H). quantitative yield 88

Intermediate 66; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d₆): δ ppm = 1.35 (s, 9 H), 7.22-7.27 (m, 1 H), 7.49- 7.57 (m, 4 H), 7.67-7.74 (m 3 H), 7.95 (s, 2 H). 69% yield 89

Intermediate 67; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 3.80 (s, 3 H), 6.98-7.02 (m, 2 H), 7.25-7.30 (m, 2 H), 7.50- 7.58 (m, 3 H), 7.68-7.74 (m, 2 H), 7.93 (s, 2 H). 91% yield 90

Intermediate 68; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 0.25 (s, 6 H), 0.96-0.99 (m, 9 H), 1.35 (s, 9 H), 6.99 (d, J = 8.62 Hz, 2 H), 7.28-7.34 (m, 2 H), 7.46 (dd, J = 8.87, 5.07 Hz, 2 H), 7.69 (d, J = 8.87 Hz, 2 H), 7.88 (s, 2 H). 92% yield 91

Intermediate 69; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.33 (s, 9 H), 2.36 (s, 3 H), 7.25 (d, J = 9.38 Hz, 4 H), 7.54 (s, 3 H), 7.69- 7.74 (m, 2 H), 7.93 (s, 2 H). 85% yield 92

Intermediate 70; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 7.22-7.32 (m, 2 H), 7.43 (br dt, J = 9.76, 2.22 Hz, 1 H), 7.48-7.60 (m, 4 H), 7.69-7.74 (m, 2 H), 7.95 (s, 2 H). 99% yield 93

Intermediate 71; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 7.33 (td, J = 7.67, 1.39 Hz, 1 H), 7.41 (ddd, J = 10.01, 8.49, 1.27 Hz, 1 H), 7.47-7.60 (m, 5 H), 7.70-7.75 (m, 2 H), 7.95 (s, 2 H). 88% yield 94

Intermediate 74; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.39 (s, 9 H), 3.85 (s, 3 H), 7.49-7.57 (m, 4 H), 7.68-7.73 (m, 2 H), 7.92 (s, 1 H), 7.98 (s, 2 H). 89% yield 95

Intermediate 75; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 7.49-7.61 (m, 4 H), 7.68- 7.75 (m, 2 H), 7.90-7.97 (m, 3 H), 8.60 (dd, J = 4.56, 1.52 Hz, 1 H), 8.63 (dd, J = 2.54, 0.51 Hz, 1 H). LC-MS (method 2) Rt = 1.24 min; MS (ESIpos): m/z = 397.5 [M + H]⁺ 85% yield 96

Intermediate 76; di-tert-butyl dicarbonate ¹H-NMR (400 MHz, DMSO- d6): δ ppm = 1.34 (s, 9 H), 7.31 (t, J = 8.74 Hz, 2 H), 7.44- 7.48 (m, 2 H), 7.64-7.68 (m, 2 H), 7.73-7.77 (m, 2 H), 7.98 (s, 2 H). LC-MS (method 2) Rt = 1.53 min; MS (ESIpos): m/z = 492.5 [M + H]⁺ preparative flash chromatography (method Z, 0-3%) 92% yield

Intermediate 97 2-bromo-1[4-(methoxymethoxy)phenyl]ethan-1-one

4-Hydroxyphenacyl bromide (2.50 g, 11.6 mmol) was provided in THF (50 mL). To this was added sodium hydride (511 mg, 60% in mineral oil, 12.8 mmol), and the suspension was stirred at rt for 1 h, after which time chloromethyl methyl ether (1.1 mL, 13.9 mmol) was added dropwise. The suspension was stirred at rt for 72 h. The reaction was stopped by the addition of a saturated aqueous solution of ammonium chloride and extracted with dichloromethane. The organics were washed with brine, dried over magnesium sulfate, filtered and concentrated under vacuum to give a brown oil. The residue was purified by normal phase column chromatography (10-80% EtOAc in heptane) to give 1.161 g (39% yield) of the title compound as a light yellow oil.

¹H-NMR (CDCl₃, 400 MHz): δ ppm=3.48 (s, 3H); 4.40 (s, 2H); 5.23 (s, 2H); 7.09 (d, 2H); 7.96 (d, 2H) LC-MS (method 2): Rt=0.79 min; MS(ESIpos) m/z=260 [M+H]⁺

Intermediate 98 (4-amino-2-anilino-1,3-thiazol-5-yl)[4-(methoxymethoxy)phenyl]methanone

Dry acetonitrile (65 mL) was provided and phenyl isothiocyanate (350 μl, 2.9 mmol; CAS-RN 103-72-0), cyanamide (146 mg, 3.47 mmol; CAS-RN 420-04-2) and 1,8-diazabicyclo[5.4.0]undec-7-en (0.75 mL, 5.1 mmol; CAS-RN 6674-22-2) were added and the solution was stirred at rt for 45 min, after which time more 1,8-diazabicyclo(5.4.0)undec-7-ene (0.75 mL, 5.1 mmol) was added, followed by 2-bromo-1-[4-(methoxymethoxy)phenyl]ethan-1-one (750 mg, 2.89 mmol in 10 mL acetonitrile; Intermediate 97). The solution was stirred at rt for 1.5 h. The mixture was partitioned between ethyl acetate and saturated aqueous sodium bicarbonate solution. The organics were washed with brine, and silica was added. The suspension was concentrated under vacuum. The residue was purified by normal phase column chromatography (10-80% EtOAc in heptane) to give 669 mg (65% yield) of the title compound as a yellow solid.

LC-MS (method 2): Rt=0.81 min; MS(ESIpos) m/z=356 [M+H]⁺

¹H-NMR (CDCl₃, 400 MHz): δ ppm=3.49 (s, 3H); 5.20 (s, 2H); 7.05 (d, 2H); 7.19 (t, 1H); 7.33-7.43 (m, 4H); 7.73 (d, 2H); 8.46 (br s, 1H); NH2 not observed.

Intermediate 99 [4-amino-2-(4-fluoroanilino)-1,3-thiazol-5-yl][4-(methoxymethoxy)phenyl]methanone

Dry acetonitrile (25 mL) was provided and 4-fluorophenyl isothiocyanate (296 mg, 1.93 mmol), cyanamide (97.4 mg, 2.32 mmol; CAS-RN 420-04-2) and 1,8-diazabicyclo(5.4.0)undec-7-ene (0.5 mL, 3.4 mmol; GAS-RN 6674-22-2) were added and the solution was stirred at rt for 45 min, after which time more 1,8-diazabicyclo(5.4.0)undec-7-ene (0.5 mL, 3.4 mmol)) was added, followed by 2-bromo-1-[4-(methoxymethoxy)phenyl]ethan-1-one (500 mg, 1.93 mmol in 10 mL acetonitrile; Intermediate 98). The solution was stirred at rt for 18 h. The mixture was evaporated to dryness under vacuum, partitioned between water and ethyl acetate and extracted into ethyl acetate. The organic layers were combined and washed with saturated aqueous sodium hydrogen carbonate solution and brine, then dried over magnesium sulfate before being filtered. Silica was added and the suspension was evaporated. This was purified by normal phase column chromatography (15-100% EtOAc in heptane) to give 620 mg (72% yield) of the title compound.

¹H-NMR (DMSO-d6, 400 MHz): δ ppm=3.43 (s, 3H); 5.29 (s, 2H); 7.12 (d, 2H); 7.25 (2H, t); 7.64-7.72 (m, 4H); 8.20 (br s, 2H); 10.79 (s, 1H).

LC-MS (method 2): Rt=0.81 min; MS(ESIpos) m/z=374 [M+H]⁺

Intermediate 100 Rac-2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic Acid

To a solution of rac-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (24 mg, 0.05 mmol; Example 81) in tetrahydrofurane (2 mL) was added aqueous sodium hydroxide solution (0.1 mL, 1 M). The reaction mixture was stirred overnight at rt.

The reaction mixture was diluted with water, aqueous hydrochloride acid (1 M) was added dropwise to adjust the pH to 3 and afterwards the mixture was extracted with dichloromethane. The organic layer was washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The residue was dissolved in acetonitrile/water (1:1) and dried by lyophilization to give 15 mg (63% yield) of the title compound.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.16 (d, J=7.35 Hz, 3H), 4.68 (s, 2H), 5.05 (br q, J=7.44 Hz, 1H), 6.89 (d, J=8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J=8.74 Hz, 2H), 7.47 (d, J=8.87 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J=8.87, 5.07 Hz, 2H), 8.08 (br s, 2H), 13.02 (br s, 1H).

LC-MS (method 1) Rt=0.87 min; MS (ESIpos): m/z=459.5 [M+H]⁺

Intermediates 100.1 and 100.2 (R)-2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic acid and (S)-2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic Acid Intermediate 100.1 2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic Acid (Enantiomer 1)

To an ice cooled solution of ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (enantiomer 1) (2.50 g, 5.14 mmol, Example 81.1) in tetrahydrofurane (26.3 mL) was added aqueous sodium hydroxide solution (5.1 mL, 1 M). The mixture was stirred overnight at rt.

The reaction mixture was diluted with water, aqueous hydrochloride acid (1 M) was added dropwise to adjust the pH to 3 and afterwards the mixture was treated with dichloromethane. The resulting precipitate was isolated by filtration, washed with water and some dichloromethane and dried by lyophilization to give 1.48 g (62% yield) of the title compound.

Intermediate 100.2 2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic Acid (Enantiomer 2)

To an ice cooled solution of ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (enantiomer 2) (260 g, 5.34 mmol; Example 81.2) in tetrahydrofurane (27.3 mL) was added aqueous sodium hydroxide solution (5.3 mL, 1 M). The mixture was stirred overnight at rt.

The reaction mixture was diluted with water, aqueous hydrochloride acid (1 M) was added dropwise to adjust the pH to 3 and afterwards the mixture was treated with dichloromethane. The resulting precipitate was isolated by filtration, washed with water and some dichloromethane and dried by lyophilization to give 0.95 g (37% yield) of the title compound.

The organic layer of the filtrate was separated, washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness to give further 695 mg (28% yield) of the title compound.

The following Intermediates were prepared from commercial starting materials stated in Table 4, below, using the procedure as for Intermediate 4, followed by purification by chromatography if needed. If no purification is specified, the respective title compound was isolated as crude product.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) if necessary. In case of a missing precipitation, the reaction mixture was extracted three times with ethyl acetate. The combined organic phases were dried over sodium sulfate, filtrated and evaporated to dryness. The crude product was purified by chromatography as stated in Table 4.

TABLE 4 Intermediates 101-197 Intermediate Chemical structure Starting number Compound name materials Analytics/purification/yield 101

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-[6- (difluoromethyl)-3- pyridyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.03 (t, J = 60 Hz, 1 H), 7.23 (t, J = 8.87 Hz, 2 H), 7.64 (dd, J = 9.12, 4.82 Hz, 2 H), 7.81 (d, J = 8.11 Hz, 1 H), 8.22 (dd, J = 7.98, 2.15 Hz, 1 H), 8.35-8.40 (m, 2 H), 8.92 (d, J = 1.52 Hz, 1 H), 10.94 (s, 1 H). LC-MS (method 1) Rt = 1.08 min; MS (ESIpos): m/z = 365.2 [M + H]⁺ 71% yield 102

2-chloro-1-fluoro-4- isothiocyanato- benzene; 2-bromo- 1-(4- pyridyl)ethanone; hydrobromide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.22 (ddd, J = 8.87, 4.56, 2.53 Hz, 1 H), 7.33-7.37 (m, 1 H), 7.47 (dd, J = 6.84, 2.53 Hz, 1 H), 7.57-7.62 (m, 2 H), 8.41 (s, 1 H), 8.50-8.55 (m, 2 H). LC-MS (method 2) Rt = 0.81 min; MS (ESIpos): m/z = 349.2 [M + H]⁺ 65% yield 103

2-chloro-1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 3.79-3.34 (m, 3 H), 7.00 (d, 2 H), 7.32-7.43 (m, 2 H), 7.66 (d, 2 H), 7.88-7.98 (m, 1 H), 8.05-8.34 (m, 1 H). LC-MS (method 2) Rt = 1.14 min; MS (ESIpos): m/z = 378.3 [M + H]⁺ 96% yield 104

1-fluoro-4- isothiocyanato- benzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H-NMR (400 MHz, DMSO-d₆): δ ppm = 7.15-7.26 (m, 3 H), 7.61- 7.68 (m, 2 H), 7.77 (t, 1H), 8.12- 8.19 (m, 1 H), 8.21-8.40 (m, 2 H), 8.53-8.57 (m, 1 H), 10.90 (br s, 1 H). LC-MS (method 2) Rt = 1.03 min; MS (ESIpos): m/z = 381.2 [M + H]⁺ 73% yield 105

1,2-difluoro-4- isothiocyanato- benzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.16-7.20 (m, 1 H), 7.24 (br d, J = 8.87 Hz, 1 H), 7.43 (d, J = 10.39 Hz, 1 H), 7.78 (t, J = 72 Hz, 1 H), 7.90-7.94 (m, 1 H), 8.17 (dd, J = 8.36, 2.53 Hz, 1 H), 8.28-8.33 (m, 2 H), 8.56 (d, J = 1.77 Hz, 1 H), 11.05 (br s, 1 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z = 399.2 [M + H]⁺ 63% yield 106

1-chloro-2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone LC-MS (method 2) Rt = 1.03 min; MS (ESIpos): m/z = 415.2 [M + H]⁺ 57% yield 107

1,2-difluoro-4- isothiocyanato- benzene; 1-[4- (benzyloxy)phenyl]- 2-bromoethanone LC-MS (method 2) Rt = 1.29 min; MS (ESIpos): m/z = 438.3 [M + H]⁺ 71% yield 108

1-chloro-2-fluoro-4- isothiocyanatobenzene; 1-[4- (benzyloxy)phenyl]- 2-bromoethanone LC-MS (method 2) Rt = 1.35 min; MS (ESIpos): m/z = 454.3 [M + H]⁺ 94% yield 109

1,2-difluoro-4- isothiocyanato- benzene; benzyl N- [4-(2- bromoacetyl)phenyl] carbamate LC-MS (method 2) Rt = 1.18 min; MS (ESIpos): m/z = 481.3 [M + H]⁺ 78% yield 110

1-fluoro-4- isothiocyanatobenzene; 1-[4- (benzyloxy)phenyl]- 2-bromoethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 5.17 (s, 2 H), 7.06-7.10 (m, 2 H), 7.19-7.23 (m, 2 H), 7.39-7.43 (m, 5 H), 7.62-7.67 (m, 4 H), 8.11-8.15 (m, 2 H), 10.75 (br s, 1 H) LC-MS (method 2) Rt = 1.30 min; MS (ESIpos): m/z = 420.2 [M + H]⁺ 86% yield 111

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(4- iodophenyl)ethanone LC-MS (method 2) Rt = 1.21 min; MS (ESIpos): m/z = 440.1 [M + H]⁺ 82% yield 112

1-fluoro-4- isothiocyanatobenzene; 2-[4- (bromoacetyl)phenoxy] ethyl acetate (Intermediate 198) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 3.73 (q, J = 5.15 Hz, 2 H), 4.03-4.07 (m, 2 H), 4.91 (t, J = 5.45 Hz, 1 H), 7.00-7.05 (m, 3 H), 7.19-7.23 (m, 2 H), 7.60- 7.65 (m, 5 H), 8.15-8.19 (m, 2 H), 10.74-10.76 (m, 1 H). LC-MS (method 2) Rt = 1.06 min; MS (ESIpos): m/z = 416.3 [M + H]⁺ 11% yield 113

1-chloro-2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(6- methoxy-3- pyridyl)ethanone LC-MS (method 2) Rt = 1.05 min; MS (ESIpos): m/z = 379.2 [M + H]⁺ 100% yield 114

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(4- phenoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.02-7.06 (m, 2 H), 7.09-7.13 (m, 2 H), 7.19-7.23 (m, 3 H), 7.43-7.47 (m, 2 H), 7.61- 7.65 (m, 2 H), 7.69-7.71 (m, 2 H), 8.19-8.21 (m, 2 H), 10.77 (br s, 1 H) LC-MS (method 2) Rt = 1.27 min; MS (ESIpos): m/z = 406.2 [M + H]⁺ 84% yield 115

1-isothiocyanato-4- methoxybenze; 2- bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 3.73-3.76 (m, 3 H), 6.93-6.96 (m, 2 H), 7.22-7.26 (m, 2 H), 7.33 (t, 1 H), 7.45-7.48 (m, 2 H), 7.70-7.73 (m, 2 H), 8.19- 8.21 (m, 2 H), 10.63 (m, 1 H) LC-MS (method 2) Rt = 1.27 min; MS (ESIpos): m/z = 406.2 [M + H]⁺ 57% yield 116

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(4- nitrophenyl)ethanone LC-MS (method 2) Rt = 0.95 min; MS (ESIpos): m/z = 359.1 [M + H]⁺ 81% yield 117

1-fluoro-4- isothiocyanatobenzene; ethanimidamide acetate (1:1); 2- bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 2.34 (s, 3 H), 3.84 (s, 3 H), 7.05 (m, 2 H), 7.21 (m, 2 H), 7.64 (m, 2 H), 7.71 (m, 2 H), 10.76 (s, 1 H) LC-MS (method 2) Rt = 1.24 min; MS (ESIpos): m/z = 343.2 [M + H]⁺ 8% yield 118

1-fluoro-4- isothiocyanatobenzene; N-[4- (bromoacetyl)phenyl] cyclopropanecarbo- xamide LC-MS (method 2) Rt = 0.77 min; MS (ESIpos): m/z = 397.3 [M + H]⁺ 69% yield 119

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[4- (morpholin-4- yl)phenyl]ethanone LC-MS (method 2) Rt = 1.04 min; MS (ESIpos): m/z = 399.2 [M + H]⁺ 66% yield 120

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-{4- [(1H-pyrazol-1- yl)methyl]phenyl} ethan-1-one, hydrogen bromide LC-MS (method 2) Rt = 1.01 min; MS (ESIpos): m/z = 394.3 [M + H]⁺ 64% yield 121

1-fluoro-4- isothiocyanatobenzene; 4-(dimethylamino) phenacyl bromide LC-MS (method 2) Rt = 1.16 min; MS (ESIpos): m/z = 357.3 [M + H]⁺ 86% yield 122

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[4- (pyrrolidin-1- yl)phenyl]ethanone LC-MS (method 2) Rt = 1.26 min; MS (ESIpos): m/z = 383.2 [M + H]⁺ 84% yield 123

1-(benzyloxy)-4- isothiocyanatobenzene; 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone LC-MS (method 2) Rt = 1.32 min; MS (ESIpos): m/z = 468.3 [M + H]⁺ 89% yield 124

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[3- (difluoromethoxy) phenyl]ethanone (Intermediate 199) LC-MS (method 2) Rt = 1.10 min; MS (ESIpos): m/z = 380.2 [M + H]⁺ 91% yield 125

1,2-difluoro-4- isothiocyanatobenzene; 2-bromo-1- (pyridin-3- yl)ethanone hydrobromide (1:1) LC-MS (method 2) Rt = 0.77 min; MS (ESIpos): m/z = 333.2 [M + H]⁺ 88% yield 126

1-fluoro-4- isothiocyanatobenzene; 4- acetamidophenacyl bromide LC-MS (method 2) Rt = 0.87 min; MS (ESIpos): m/z = 371.3 [M + H]⁺ 58% yield 127

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(2- chloropyridin-4- yl)ethanone LC-MS (method 2) Rt = 0.86 min; MS (ESIpos): m/z = 349.2 [M + H]⁺ 88% yield 128

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(2- methylpyridin-4- yl)ethanone hydrobromide (1:1) LC-MS (method 2) Rt = 0.80 min; MS (ESIpos): m/z = 329.2 [M + H]⁺ 80% yield 129

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[2- (difluoromethyl)-4- pyridyl]ethanone LC-MS (method 2) Rt = 0.87 min; MS (ESIpos): m/z = 365.2 [M + H]⁺ 78% yield 130

4- isothiocyanatopyridine; 2-bromo-1- phenylethanone LC-MS (method 2) Rt = 0.69 min; MS (ESIpos): m/z = 297.2 [M + H]⁺ 17% yield 131

1-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(2- methoxypyridin-4- yl)ethanone LC-MS (method 2) Rt = 0.91 min; MS (ESIpos): m/z = 345.2 [M + H]⁺ 80% yield 132

1-fluoro-4- isothiocyanatobenzene; benzyl N-[4-(2- bromoacetyl)phenyl] carbamate LC-MS (method 2) Rt = 1.20 min; MS (ESIpos): m/z = 463.3 [M + H]⁺ 72% yield 133

2-fluoro-4- isothiocyanato-1- methoxybenze; 2- bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.75 (s, 1H), 8.23 (br s, 2H), 7.68-7.75 (m, 1H), 7.64-7.68 (m, 2H), 7.44-7.52 (m, 3H), 7.13- 7.22 (m, 2H), 3.82 (s, 3H). LC-MS (method 2} Rt = 1.08 min MS (ESIpos): m/z = 344.6 [M + H]⁺ 96% yield 134

2-fluoro-4- isothiocyanatobenzo nitrile; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.42 (br s, 1H), 8.28 (br s, 2H), 8.10 (dd, J = 12.5, 1.9 Hz, 1H), 7.86 (dd, J = 8.6, 7.9 Hz, 1H), 7.67-7.72 (m, 2H), 7.46-7.56 (m, 3H), 7.39 (dd, J = 8.6, 2.0 Hz, 1H). LC-MS (method 2) Rt = 0.9 min MS (ESIpos): m/z = 339.5 [M + H]⁺ 87% yield 135

1-bromo-4- isothiocyanatobenzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.89 (br s, 1H), 8.22 (br s, 2H), 7.64-7.70 (m, 2H), 7.57-7.64 (m, 2H), 7.44-7.57 (m, 5H). LC-MS (method 2) Rt = 1.18 min MS (ESIpos): m/z = 375.2 [M + H]⁺ 100% yield 136

2-chloro-1- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHZ, DMSO-d₆) δ ppm = 10.97 (br s, 1H), 8.26 (br s, 2H), 8.05 (d, J = 2.5 Hz, 1H), 7.64-7.70 (m, 2H), 7.45-7.54 (m, 4H), 7.36 (d, J = 8.9 Hz, 1H), 7.20 (t, J = 73.3 Hz, 1H). LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 396.3 [M + H]⁺ 92% yield 137

1-ethoxy-4- isothiocyanatobenzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.58 (br s, 1H), 7.69-8.55 (m, 2H), 7.60-7.66 (m, 2H), 7.40- 7.51 (m, 5H), 6.88-6.95 (m, 2H), 4.00 (q, J = 7.1 Hz, 2H), 1.31 (t, J = 7.0 Hz, 3H). LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 340.4 [M + H]⁺ 100% yield 138

5-isothiocyanato- 1,3-benzodioxole; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.64 (s, 1H), 8.20 (br s, 2H), 7.62-7.67 (m, 2H), 7.43-7.52 (m, 3H), 7.32-7.37 (m, 1H), 6.88- 6.95 (m, 2H), 6.02 (s, 2H). LC-MS (method 2) Rt = 0.99 min MS (ESIpos): m/z = 340.2 [M + H]⁺ 57% yield 139

2,2-difluoro-5- isothiocyanato-1,3- benzodioxole; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.95 (br s, 1H), 8.24 (br s, 2H), 7.94 (d, J = 2.0 Hz, 1H), 7.64-7.69 (m, 2H), 7.45-7.53 (m, 3H), 7.40 (d, J = 8.6 Hz, 1H), 7.24 (dd, J = 8.7, 2.2 Hz, 1H). LC-MS (method 2) Rt = 1.17 min MS (ESIpos): m/z = 376.4 [M + H]⁺ 78% yield 140

1-(difluoromethoxy)- 2-fluoro-4- isothiocyanatobenzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.00 (s, 1H), 8.26 (br s, 2H), 7.97 (dd, J = 12.9, 2.5 Hz, 1H), 7.65-7.70 (m, 2H), 7.45-7.54 (m, 3H), 7.33-7.39 (m, 1H), 7.24- 7.29 (m, 1H), 7.18 (t, J = 73.3 Hz, 1H). LC-MS (method 2) Rt = 1.08 min MS (ESIpos): m/z = 380.2 [M + H]⁺ 92% yield 141

1-(benzyloxy)-4- isothiocyanatobenzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.61 (br s, 1H), 8.17 (br s, 2H), 7.64 (dd, J = 7.6, 1.8 Hz, 2H), 7.42-7.50 (m, 7H), 7.36-7.42 (m, 2H), 7.30-7.36 (m, 1H), 6.99- 7.04 (m, 2H), 5.09 (S, 2H). LC-MS (method 2) Rt = 1.3 min MS (ESIpos): m/z = 402.4 [M + H]⁺ 98% yield 142

2-chloro-1- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.94 (br s, 1H), 8.21 (br s, 2H), 8.06 (d, J = 2.5 Hz, 1H), 7.66-7.71 (m, 2H), 7.48 (dd, J = 8.9, 2.5 Hz, 1H), 7.36 (d, J = 8.9 Hz, 1H), 7.20 (t, J = 73.8 Hz, 1H), 7.02-7.05 (m, 1H), 7.00-7.02 (m, 1H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.17 min MS (ESIpos): m/z = 426.2 [M + H]⁺ 99% yield 143

2-chloro-1- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.00 (br s, 1H), 8.14-8.42 (m, 2H), 8.05 (d, J = 2.5 Hz, 1H), 7.73-7.78 (m, 2H), 7.48 (dd, J = 9.0, 2.7 Hz, 1H), 7.38 (d, J = 6.1 Hz, 1H), 7.35 (t, J = 73.8 Hz, 1H), 7.24-7.29 (m, 2H), 7.20 (t, J = 73.3 Hz, 1H). LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 462.2 [M + H]⁺ 87% yield 144

2-chloro-1- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1-(4- chlorophenyl)ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.02 (br s, 1H), 8.31 (br s, 2H), 8.05 (d, J = 2.3 Hz, 1H), 7.67-7.72 (m, 2H), 7.53-7.58 (m, 2H), 7.47 (dd, J = 9.0, 2.7 Hz, 1H), 7.36 (d, J = 8.9 Hz, 1H), 7.20 (t, J = 73.5 Hz, 1H). LC-MS (method 2) Rt = 1.20 min MS (ESIpos): m/z = 430.2 [M + H]⁺ 100% yield 145

2-chloro-1- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1-(pyridin- 4-yl)ethanone hydrobromide ¹H NMR (400 MHz DMSO-d₆) δ ppm = 11.08 (br s, 1H), 8.70-8.74 (m, 2H), 8.42 (br s, 2H), 8.06 (d, J = 2.5 Hz, 1H), 7.56-7.60 (m, 2H), 7.46-7.51 (m, 1H), 7.37 (d, J = 9.1 Hz, 1H), 7.21 (t, J = 73.3 Hz, 1H). LC-MS (method 2) Rt = 0.82 min MS (ESIpos): m/z = 397.2 [M + H]⁺ 45% yield 146

2-chloro-1- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.07 (br s, 1H), 8.55-8.58 (m, 1H), 8.34 (br s, 2H), 8.18 (dd, J = 8.6, 2.5 Hz, 1H), 8.04 (d, J = 2.0 Hz, 1H), 7.78 (t, J = 72.5 Hz, 1H), 7.47 (dd, J = 9.0, 2.7 Hz, 1H), 7.37 (d, J = 8.9 Hz, 1H), 7.21 (t, J = 73.5 Hz, 1H), 7.18-7.21 (m, 1H). LC-MS (method 2) Rt = 1.06 min MS (ESIpos); m/z = 463.2 [M + H]⁺ 92% yield 147

1-(difluoromethoxy)- 2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.99 (br s, 1H), 8.20 (br s, 2H), 7.97 (dd, J = 13.1, 2.4 Hz, 1H), 7.66-7.71 (m, 2H), 7.33-7.39 (m, 1H), 7.24-7.30 (m, 1H), 7.17 (t, J = 73.3 Hz, 1H), 7.00-7.05 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.13 min MS (ESIpos); m/z = 410.2 [M + H]⁺ 96% yield 148

1-(difluoromethoxy)- 2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-{4- chlorophenyl)ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.06 (br s, 1H), 8.30 (br s, 2H), 7.96 (dd, J = 12.9, 2.5 Hz, 1H), 7.68-7.72 (m, 2H), 7.53-7.58 (m, 2H), 7.33-7.39 (m, 1H), 7.24- 7.29 (m, 1H), 7.18 (t, J = 73.3 Hz, 1H). LC-MS (method 2) Rt = 1.18 min MS (ESIpos); m/z = 414.2 [M + H]⁺ 95% yield 149

1-(difluoromethoxy)- 2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.03 (br s, 1H), 8.28 (br s, 2H), 7.96 (dd, J = 12.9, 2.3 Hz, 1H), 7.73-7.78 (m, 2H), 7.32-7.55 (m, 2H), 6.99-7.30 (m, 4H). LC-MS (method 2) Rt = 1.14 min MS (ESIpos); m/z = 446.2 [M + H]⁺ 88% yield 150

1-(difluoromethoxy)- 2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-(pyridin- 4-yl)ethanone hydrobromide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.12 (s, 1H), 8.70-8.74 (m, 2H), 8.41 (br s, 2H), 7.97 (dd, J = 12.9, 2.5 Hz, 1H), 7.57-7.60 (m, 2H), 7.34-7.38 (m, 1H), 7.25- 7.30 (m, 1H), 7.18 (t, J = 73.3 Hz, 1H). LC-MS (method 2) Rt = 0.8 min MS (ESIpos): m/z = 381.2 [M + H]⁺ 15% yield 151

1-(difluoromethoxy)- 2-fluoro-4- isothiocyanatobenzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.02-11.23 (m, 1H), 8.57 (d, J = 2.3 Hz, 1H), 8.25-8.41 (m, 2H), 8.18 (dd, J = 8.5, 2.4 Hz, 1H), 7.92-7.98 (m, 1H), 7.78 (t, J = 72.2 Hz, 1H), 7.33-7.40 (m, 1H), 7.24-7.30 (m, 1H), 7.20 (d, J = 9.1 Hz, 1H), 7.18 (t, J = 73.5 Hz, 1H). LC-MS (method 2) Rt = 1.06 min MS (ESIpos): m/z = 447.2 [M + H]⁺ 86% yield 152

2-fluoro-4- isothiocyanato-1- (trifluoromethoxy) benzene (Intermediate 200); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.12 (br s, 1H), 8.26 (br s, 2H), 8.08 (dd, J = 12.9, 2.5 Hz, 1H), 7.66-7.70 (m, 2H), 7.45-7.59 (m, 4H), 7.29-7.34 (m, 1H). LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 398.2 [M + H]⁺ 96% yield 153

2-chloro-4- isothiocyanato-1- (trifluoromethoxy) benzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.08 (br s, 1H), 8.28 (br s, 2H), 8.16 (d, J = 2.3 Hz, 1H), 7.65-7.72 (m, 2H), 7.45-7.59 (m, 5H). LC-MS (method 2) Rt = 1.22 min MS (ESIpos): m/z = 414.2 [M + H]⁺ 91% yield 154

2,2-difluoro-5- isothiocyanato-1,3- benzodioxole; 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.92 (br s, 1H), 8.17 (br s, 2H), 7.95 (d, J = 2.0 Hz, 1H), 7.65-7.70 (m, 2H), 7.40 (d, J = 8.6 Hz, 1H), 7.24 (dd, J = 8.7, 2.2 Hz, 1H), 6.99-7.05 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 406.2 [M + H]⁺ 89% yield 155

2,2-difluoro-5- isothiocyanato-1,3- benzodioxole; 2-bromo-1-(4- chlorophenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.00 (br d, J = 1.3 Hz, 1H), 8.28 (br s, 2H), 7.94 (d, J = 2.0 Hz, 1H), 7.66-7.73 (m, 2H), 7.51-7.58 (m, 2H), 7.40 (d, J = 8.6 Hz, 1H), 7.24 (dd, J = 8.9, 2.3 Hz, 1H). LC-MS (method 2) Rt = 1.25 min MS (ESIpos): m/z = 410.2 [M + H]⁺ 93% yield 156

2,2-difluoro-5- isothiocyanato-1,3- benzodioxole; 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.98 (br s, 1H), 8.24 (br s, 2H), 7.93 (d, J = 1.5 Hz, 1H), 7.71-7.78 (m, 2H), 7.40 (d, J = 8.6 Hz, 1H), 7.35 (t, J = 73.8 Hz, 1H), 7.21-7.29 (m, 3H). LC-MS (method 2) Rt = 1.23 min MS (ESIpos): m/z = 442.2 [M + H]⁺ 61% yield 157

2,2-difluoro-5- isothiocyanato-1,3- benzodioxole; 2-bromo-1-(pyridin- 4-yl)ethanone hydrobromide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.06 (s, 1H), 8.69-8.74 (m, 2H), 8.38 (br s, 2H), 7.94 (d, J = 1.8 Hz, 1H), 7.56-7.60 (m, 2H), 7.41 (d, J = 8.9 Hz, 1H), 7.25 (dd, J = 8.7, 2.2 Hz, 1H). LC-MS (method 2) Rt = 0.85 min MS (ESIpos): m/z = 377.2 [M + H]⁺ 13% yield 158

2,2-difluoro-5- isothiocyanato-1,3- benzodioxole; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.05 (br s, 1H), 8.54-8.58 (m, 1H), 8.31 (br s, 2H), 8.17 (dd, J = 8.6, 2.5 Hz, 1H), 7.94 (d, J = 1.8 Hz, 1H), 7.78 (t, J = 72.5 Hz, 1H), 7.41 (d, J = 8.9 Hz, 1H), 7.25 (dd, J = 8.9, 2.3 Hz, 1H), 7.17- 7.22 (m, 1H). LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 443.2 [M + H]⁺ 85% yield 159

2-fluoro-4- isothiocyanato-1- (trifluoromethoxy) benzene (Intermediate 200); 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.10 (br s, 1H), 8.20 (br s, 2H), 8.08 (dd, J = 12.9, 2.5 Hz, 1H), 7.66-7.72 (m, 2H), 7.55 (td, J = 8.9, 1.0 Hz, 1H), 7.30-7.35 (m, 1H), 7.00-7.05 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.24 min MS (ESIpos): m/z = 428.2 [M + H]⁺ 90% yield 160

2-fluoro-4- isothiocyanato-1- (trifluoromethoxy) benzene (Intermediate 200); 2-bromo-1-(4- chlorophenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.10 (br s, 1H), 8.20 (br s, 2H), 8.08 (dd, J = 12.9, 2.5 Hz, 1H), 7.66-7.72 (m, 2H), 7.55 (td, J = 9.0, 1.0 Hz, 1H), 7.30-7.35 (m, 1H), 7.00-7.06 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.23 min MS (ESIpos): m/z = 432.2 [M + H]⁺ 91% yield 161

2-fluoro-4- isothiocyanato-1- (trifluoromethoxy) benzene (Intermediate 200); 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.15 (br s, 1H), 8.28 (br s, 2H), 8.07 (dd, J = 12.9, 2.5 Hz, 1H), 7.73-7.80 (m, 2H), 7.51-7.62 (m, 1H), 7.36 (t, J = 73.8 Hz, 1H), 7.30-7.34 (m, 1H), 7.25-7.30 (m, 2H). LC-MS (method 2) Rt = 1.24 min MS (ESIpos): m/z = 464.2 [M + H]⁺ 86% yield 162

2-fluoro-4- isothiocyanato-1- (trifluoromethoxy) benzene (Intermediate 200); 2-bromo-1-(pyridin- 4-yl)ethanone hydrobromide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.23 (s, 1H), 8.70-8.75 (m, 2H), 8.42 (br s, 2H), 8.08 (dd, J = 12.9, 2.5 Hz, 1H), 7.53-7.62 (m, 3H), 7.30-7.35 (m, 1H). LC-MS (method 2) Rt = 0.89 min MS (ESIpos): m/z = 399.2 [M + H]⁺ 21% yield 163

2-fluoro-4- isothiocyanato-1- (trifluoromethoxy) benzene (Intermediate 200); 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.22 (br s, 1H), 8.55-8.61 (m, 1H), 8.34 (br s, 2H), 8.19 (dd, J = 8.5, 2.4 Hz, 1H), 8.07 (dd, J = 12.9, 2.5 Hz, 1H), 7.78 (t, J = 72.5 Hz, 1H), 7.56 (td, J = 8.9, 1.0 Hz, 1H), 7.30-7.36 (m, 1H), 7.18-7.22 (m, 1H). LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 465.2 [M + H]⁺ 84% yield 164

2-chloro-4- isothiocyanato-1- (trifluoromethoxy) benzene; 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.06 (br s, 1H), 8.05-8.37 (m, 3H), 7.66-7.72 (m, 2H), 7.50- 7.58 (m, 2H), 7.00-7.05 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.22 min MS (ESIpos): m/z = 444.2 [M + H]⁺ 79% yield 165

2-chloro-4- isothiocyanato-1- (trifluoromethoxy) benzene; 2-bromo-1-(4- chlorophenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.87-7.99 (m, 1H), 7.64- 7.71 (m, 2H), 7.48-7.54 (m, 2H), 7.43 (s, 1H), 7.37 (brd, J = 0.8 Hz, 1H). LC-MS (method 2) Rt = 1.3 min MS (ESIpos): m/z = 448.2 [M + H]⁺ 53% yield 166

2-chloro-4- isothiocyanato-1- (trifluoromethoxy) benzene; 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.11 (br s, 1H), 8.29 (br s, 2H), 8.15 (d, J = 2.5 Hz, 1H), 7.73-7.79 (m, 2H), 7.50-7.58 (m, 2H), 7.36 (t, J = 73.2 Hz, 1H), 7.25-7.30 (m, 2H). LC-MS (method 2) Rt = 1.24 min MS (ESIpos): m/z = 480.2 [M + H]⁺ 71% yield 167

2-chloro-4- isothiocyanato-1- (trifluoromethoxy) benzene; 2-bromo-1-(pyridin- 4-yl)ethanone hydrobromide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.19 (s, 1H), 8.70-8.75 (m, 2H), 8.43 (br s, 2H), 8.17 (d, J = 2.3 Hz, 1H), 7.51-7.61 (m, 4H). LC-MS (method 2) Rt = 0.93 min MS (ESIpos): m/z = 415.2 [M + H]⁺ 47% yield 168

2-chloro-4- isothiocyanato-1- (trifluoromethoxy) benzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.20 (br s, 1H), 8.54-8.59 (m, 1H), 8.21-8.52 (m, 2H), 8.18 (dd, J = 8.4, 2.5 Hz, 1H), 8.11 (br s, 1H), 7.78 (t, J = 72.5 Hz, 1H), 7.46-7.58 (m, 2H), 7.17-7.21 (m, 1H). LC-MS (method 2) Rt = 1.19 min MS (ESIpos): m/z = 481.2 [M + H]⁺ 34% yield 169

5-isothiocyanato-2- methoxypyridine; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.69 (br s, 1H), 8.40 (d, J = 2.5 Hz, 1H), 7.96-8.38 (m, 2H), 7.93 (dd, J = 8.9, 2.8 Hz, 1H), 7.62-7.67 (m, 2H), 7.43-7.52 (m, 3H), 6.84-6.88 (m, 1H), 3.83 (s, 3H). LC-MS (method 2) Rt = 0.91 min MS (ESIpos): m/z = 327.2 [M + H]⁺ 87% yield 170

5-isothiocyanato-2- (trifluoromethoxy) pyridine (Intermediate 201); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.10 (br s, 1H), 8.67 (d, J = 2.8 Hz, 1H), 8.27 (dd, J = 8.9, 2.8 Hz, 3H), 7.66-7.70 (m, 2H), 7.46-7.53 (m, 3H), 7.34 (d, J = 8.9 Hz, 1H). LC-MS (method 2) Rt = 0.96 min MS (ESIpos): m/z = 381.2 [M + H]⁺ 82% yield 171

2-(difluoromethoxy)-5- isothiocyanatopyridine (Intermediate 202); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.94 (br s, 1H), 8.56 (d, J = 2.8 Hz, 1H), 8.16 (dd, J = 8.9, 2.8 Hz, 3H), 7.67 (dd, J = 7.6, 1.8 Hz, 2H), 7.65 (t, J = 73.0 Hz, 1H), 7.45-7.53 (m, 3H), 7.14 (d, J = 8.9 Hz, 1H). LC-MS (method 2) Rt = 0.93 min MS (ESIpos): m/z = 363.2 [M + H]⁺ 90% yield 172

5-isothiocyanato-2- (trifluoromethoxy) pyridine; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.26-11.46 (m, 1H), 8.93 (d, J = 2.5 Hz, 1H), 8.40 (dd, J = 8.5, 2.2 Hz, 1H), 8.25 (br s, 2H), 7.89 (d, J = 8.6 Hz, 1H), 7.67- 7.72 (m, 2H), 7.46-7.56 (m, 3H). LC-MS (method 2) Rt = 0.88 min MS (ESIpos): m/z = 365.2 [M + H]⁺ 69% yield 173

2-(difluoromethyl)-5- isothiocyanatopyridine (Intermediate 203); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.21 (br s, 1H), 8.88 (d, J = 2.3 Hz, 1H), 8.15-8.37 (m, 3H), 7.66-7.72 (m, 3H), 7.46-7.55 (m, 3H), 6.92 (t, J = 55.3 Hz, 1H). LC-MS (method 2) Rt = 0.79 min MS (ESIpos): m/z = 347.2 [M + H]⁺ 66% yield 174

2-chloro-5- isothiocyanatopyridine; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.09 (br s, 1H), 8.68 (d, J = 2.5 Hz, 1H), 8.12-8.37 (m, 3H), 7.65-7.71 (m, 2H), 7.45-7.55 (m, 4H). LC-MS (method 2) Rt = 0.79 min MS (ESIpos): m/z = 331.2 [M + H]⁺ 60% yield 175

2-fluoro-5- isothiocyanatopyridine; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.99 (br s, 1H), 8.52 (dd, J = 2.5, 1.3 Hz, 1H), 7.96-8.46 (m, 3H), 7.65-7.70 (m, 2H), 7.44- 7.54 (m, 3H), 7.22 (dd, J = 8.9, 3.0 Hz, 1H). LC-MS (method 2) Rt = 0.79 min MS (ESIpos): m/z = 315.2 [M + H]⁺ 70% yield 176

5-isothiocyanato-2- methylpyridine; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.83 (br s, 1H), 8.63 (d, J = 2.5 Hz, 1H), 8.03-8.52 (m, 2H), 8.00 (dd, J = 8.4, 2.5 Hz, 1H), 7.63-7.70 (m, 2H), 7.44-7.53 (m, 3H), 7.25 (d, J = 8.4 Hz, 1H), 2.43 (s, 3H). LC-MS (method 2) Rt = 0.81 min MS (ESIpos): m/z = 311.2 [M + H]⁺ 88% yield 177

1-fluoro-4- isothiocyanato-2- (trifluoromethoxy) benzene (Intermediate 204); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.01 (br s, 1H), 8.09-8.42 (m, 2H), 7.99 (dd, J = 7.1, 1.3 Hz, 1H), 7.66-7.70 (m, 2H), 7.56-7.61 (m, 1H), 7.45-7.55 (m, 4H). LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 398.2 [M + H]⁺ 94% yield 178

1-chloro-4- isothiocyanato-2- (trifluoromethoxy) benzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.12 (br s, 1H), 8.25 (br s, 2H), 8.01 (d, J = 0.8 Hz, 1H), 7.60-7.71 (m, 4H), 7.45-7.55 (m, 3H). LC-MS (method 2) Rt = 1.28 min MS (ESIpos): m/z = 414.2 [M + H]⁺ 90% yield 179

2-(difluoromethoxy)- 1-fluoro-4- isothiocyanatobenzene (Intermediate 205); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.91 (br s, 1H), 8.06-8.43 (m, 2H), 7.74 (dd, J = 6.7, 2.2 Hz, 1H), 7.64-7.70 (m, 2H), 7.45-7.53 (m, 4H), 7.37-7.42 (m, 1H), 7.25 (t, J = 73.0 Hz, 1H). LC-MS (method 2) Rt = 1.1 min MS (ESIpos): m/z = 380.2 [M + H]⁺ 95% yield 180

1-chloro-2- (difluoromethoxy)-4- isothiocyanatobenzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.03 (br s, 1H), 8.23 (br s, 2H), 7.75 (d, J = 2.0 Hz, 1H), 7.65-7.70 (m, 2H), 7.46-7.58 (m, 5H), 7.27 (t, J = 73.0 Hz, 1H). LC-MS (method 2) Rt = 1.12 min MS (ESIpos): m/z = 396.2 [M + H]⁺ 98% yield 181

2-fluoro-1- isothiocyanato-4- (trifluoromethoxy) benzene (Intermediate 206); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.69 (br s, 1H), 8.25 (br t, J = 9.0 Hz, 3H), 7.63-7.69 (m, 2H), 7.43-7.56 (m, 4H), 7.28 (dd, J = 9.1, 1.0 Hz, 1H). LC-MS (method 2) Rt = 1.02 min MS (ESIpos): m/z = 398.2 [M + H]⁺ 100% yield 182

2-chloro-1- isothiocyanato-4- (trifluoromethoxy) benzene; 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 9.25-9.70 (m, 1H), 8.46 (s, 1H), 7.55-7.68 (m, 3H), 7.32-7.46 (m, 4H), 7.16-7.25 (m, 1H). LC-MS (method 2) Rt = 1.05 min MS (ESIpos): m/z = 414.2 [M + H]⁺ 45% yield 183

4-(difluoromethoxy)- 2-fluoro-1- isothiocyanatobenzene (Intermediate 207); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.25-10.79 (m, 1H), 7.90- 8.47 (m, 3H), 7.61-7.66 (m, 2H), 7.40-7.50 (m, 4H), 7.24-7.29 (m, 2H), 7.02-7.09 (m, 1H). LC-MS (method 2) Rt = 0.98 min MS (ESIpos): m/z = 380.2 [M + H]⁺ 100% yield 184

2-chloro-4- (difluoromethoxy)-1- isothiocyanatobenzene (Intermediate 208); 2-bromo-1- phenylethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.43 (br s, 1H), 7.88-8.41 (m, 2H), 7.84 (d, J = 8.6 Hz, 1H), 7.62 (dd, J = 7.6, 1.8 Hz, 2H), 7.41-7.48 (m, 4H), 7.30 (t, J = 73.8 Hz, 1H), 7.21 (dd, J = 8.7, 2.7 Hz, 1H). LC-MS (method 2) Rt = 1.02 min MS (ESIpos): m/z = 396.2 [M + H]⁺ 99% yield 185

1-(difluoromethyl)-4- isothiocyanatobenzene; 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.97 (br s, 1H), 8.17 (br s, 2H), 7.77 (d, J = 8.6 Hz, 2H), 7.66-7.71 (m, 2H), 7.56 (d, J = 8.6 Hz, 2H), 7.00-7.05 (m, 2H), 6.98 (t, J = 56.0 Hz, 1H), 3.82 (s, 3H). LC-MS (method 2) Rt = 1.07 min MS (ESIpos): m/z = 376.1 [M + H]⁺ 88% yield 186

1-(difluoromethyl)-4- isothiocyanatobenzene; 2-bromo-1-(4- chlorophenyl)ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.06 (br s, 1H), 8.28 (br s, 2H), 7.75 (br d, J = 8.4 Hz, 2H), 7.68-7.72 (m, 2H), 7.52-7.58 (m, 4H), 6.98 (t, J = 56.0 Hz, 1H). LC-MS (method 2) Rt = 1.11 min MS (ESIpos): m/z = 380.1 [M + H]⁺ 94% yield 187

1-(difluoromethyl)-4- isothiocyanatobenzene; 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.03 (br s, 1H), 8.24 (br s, 2H), 7.73-7.78 (m, 4H), 7.56 (d, J = 8.6 Hz, 2H), 7.35 (t, J = 73.8 Hz, 1H), 7.24-7.29 (m, 2H), 6.99 (t, J = 56.0 Hz, 1H). LC-MS (method 2) Rt = 1.09 min MS (ESIpos): m/z = 412.1 [M + H]⁺ 91% yield 188

1-(difluoromethyl)-4- isothiocyanatobenzene; 2-bromo-1-(pyridin- 4-yl)ethanone hydrobromide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.83-11.29 (m, 1H), 8.67- 8.76 (m, 2H), 8.39 (br s, 2H), 7.77 (d, J = 8.6 Hz, 2H), 7.53-7.63 (m, 4H), 6.99 (t, J = 55.8 Hz, 1H). LC-MS (method 2) Rt = 0.72 min MS (ESIpos): m/z = 347.1 [M + H]⁺ 39% yield 189

1-(difluoromethyl)-4- isothiocyanatobenzene; 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.10 (br s, 1H), 8.56-8.60 (m, 1H), 8.32 (br s, 2H), 8.18 (dd, J = 8.4, 2.5 Hz, 1H), 7.78 (t, J = 72.5 Hz, 1H), 7.77 (d, J = 8.4 Hz, 2H), 7.57 (d, J = 8.6 Hz, 2H), 7.18-7.22 (m, 1H), 6.99 (t, J = 55.8 Hz, 1H). LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 413 [M + H]⁺ 89% yield 190

5-isothiocyanato-2- (trifluoromethoxy) pyridine (Intermediate 201); 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.07 (br s, 1H), 8.67 (d, J = 2.8 Hz, 1H), 8.27 (dd, J = 8.9, 2.8 Hz, 1H), 8.17 (br s, 2H), 7.65- 7.71 (m, 2H), 7.34 (d, J = 8.9 Hz, 1H), 7.00-7.05 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 0.95 min MS (ESIpos): m/z = 411.2 [M + H]⁺ 63% yield 191

5-isothiocyanato-2- (trifluoromethoxy) pyridine (Intermediate 201); 2-bromo-1-(4- chlorophenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.18 (br s, 1H), 8.64 (d, J = 2.5 Hz, 1H), 8.25 (br dd, J = 8.9, 3.0 Hz, 3H), 7.67-7.73 (m, 2H), 7.53-7.58 (m, 2H), 7.33 (d, J = 8.9 Hz, 1H). LC-MS (method 2) Rt = 0.98 min MS (ESIpos): m/z = 415.2 [M + H]⁺ 68% yield 192

5-isothiocyanato-2- (trifluoromethoxy) pyridine (Intermediate 201); 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.13 (br s, 1H), 8.67 (d, J = 2.8 Hz, 1H), 8.27 (dd, J = 8.9, 2.8 Hz, 3H), 7.72-7.79 (m, 2H), 7.16-7.55 (m, 4H). LC-MS (method 2) Rt = 0.97 min MS (ESIpos): m/z = 447.4 [M + H]⁺ 90% yield 193

5-isothiocyanato-2- (trifluoromethoxy) pyridine (Intermediate 201); 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.23 (br s, 1H), 8.65 (d, J = 2.5 Hz, 1H), 8.55-8.59 (m, 1H), 8.21-8.48 (m, 3H), 8.18 (dd, J = 8.6, 2.5 Hz, 1H), 7.78 (t, J = 72.9 Hz, 1H), 7.34 (d, J = 8.9 Hz, 1H), 7.17-7.22 (m, 1H). LC-MS (method 2) Rt = 0.85 min MS (ESIpos): m/z = 448.2 [M + H]⁺ 70% yield 194

2-(difluoromethoxy)-5- isothiocyanatopyridine (Intermediate 202); 2-bromo-1-(4- methoxyphenyl) ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 10.91 (s, 1H), 8.57 (d, J = 2.5 Hz, 1H), 8.17 (dd, J = 8.9, 2.8 Hz, 3H), 7.65-7.70 (m, 2H), 7.65 (t, J = 73.0 Hz, 1H), 7.13 (d, J = 8.9 Hz, 1H), 6.99-7.05 (m, 2H), 3.82 (s, 3H). LC-MS (method 2) Rt = 0.94 min MS (ESIpos): m/z = 393.2 [M + H]⁺ 86% yield 195

2-(difluoromethoxy)-5- isothiocyanatopyridine (Intermediate 202); 2-bromo-1-(4- chlorophenyl)ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 11.00 (br s, 1H), 8.52 (d, J = 2.5 Hz, 1H), 8.14 (dd, J = 8.9, 2.8 Hz, 3H), 7.66-7.71 (m, 2H), 7.65 (t, J = 73.3 Hz, 1H), 7.54 (d, J = 8.6 Hz, 2H), 7.12 (d, J = 8.9 Hz, 1H). LC-MS (method 2) Rt = 0.94 min MS (ESIpos): m/z = 397.1 [M + H]⁺ 82% yield 196

2-(difluoromethoxy)-5- isothiocyanatopyridine (Intermediate 202); 2-bromo-1-[4- (difluoromethoxy) phenyl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.56-10.12 (m, 1H), 7.70- 8.09 (m, 2H), 7.65 (d, J = 8.6 Hz, 2H), 7.57 (t, J = 73.8 Hz, 1H), 7.27 (t, J = 74.1 Hz, 1H), 7.14 (d, J = 8.6 Hz, 2H), 6.88 (d, J = 8.9 Hz, 1H). LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 429.1 [M + H]⁺ 87% yield 197

2-(difluoromethoxy)-5- isothiocyanatopyridine (Intermediate 202); 2-bromo-1-[6- (difluoromethoxy) pyridin-3-yl]ethanone ¹H NMR (400 MHz, DMSO-d₆) δ ppm = ¹H NMR (400 MHz, DMSO-d6, 22° C.): Shift = 11.04 (br s, 1H), 8.53-8.59 (m, 2H), 8.31 (br s, 2H), 8.14-8.19 (m, 2H), 7.78 (t, J = 72.5 Hz, 1H), 7.65 (t, J = 73.0 Hz, 1H), 7.20 (dd, J = 8.6, 0.8 Hz, 1H), 7.14 (d, J = 8.9 Hz, 1H). LC-MS (method 2) Rt = 0.92 min MS (ESIpos): m/z = 430.4 [M + H]⁺ 86% yield

Intermediate 198 2-[4-(2-bromoacetyl)phenoxy]ethyl Acetate

2-(4-acetylphenoxy)ethyl acetate (940 mg, 4.23 mmol) in THF (12.5 mL) at 0° C. was treated with phenyl trimethyl ammonium tribromide (1.59 g, 4.23 mmol). The reaction mixture was stirred at rt overnight, diluted with water and extracted three times with dichloromethane. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The crude product was purified by Biotage (method X) to give 237 mg (0.79 mmol, 18% yield) of the title compound.

LC-MS (method 1): Rt=1.05 min; MS(ESIpos) m/z=300.0 [M+H]⁺

Intermediate 199 2-bromo-1-[3-(difluoromethoxy)phenyl]ethanone

3′-(difluoromethoxy)acetophenone (1 g, 5.37 mmol) in THF (15 mL) at 0° C. was treated with phenyl trimethyl ammonium tribromide (2.02 g, 5.37 mmol). The reaction mixture was stirred at rt overnight, diluted with water and extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The crude product (quant.) was used without further purification.

LC-MS (method 1): Rt=1.14 min; MS(ESIpos) m/z=264.9 [M+H]⁺

Intermediate 200 2-Fluoro-4-isothiocyanato-1-(trifluoromethoxy)benzene

3-fluoro-4-(trifluoromethoxy)aniline (560 mg, 2.81 mmol) was suspended in dichloromethane (12 mL) followed by the addition of triethylamine (1.76 mL, 12.65 mmol). The mixture was cooled to 0° C. and carbonothioyl dichloride (356 mg, 3.09 mmol) diluted in dichloromethane (1.5 mL) was added slowly. After removal of the ice bath the batch was stirred at room temperature for one hour. Water (12 mL) and dichloromethane (7.5 mL) were added, the organic layer was separated, and the aqueous phase was extracted with dichloromethane. The combined organic phases were filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The crude material was purified via Biotage (hexanes/ethyl acetate) to yield 350 mg (1.36 mmol, 48%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=7.79 (dd, J=11.0, 2.4 Hz, 1H), 7.65-7.71 (m, 1H), 7.43 (ddd, J=8.9, 2.5, 1.5 Hz, 1H).

The following Intermediates were prepared from the starting materials stated in Table 5, below, using the procedure as for 2-fluoro-4-isothiocyanato-1-(trifluoromethoxy)benzene/Intermediate 200.

TABLE 5 Intermediates 201-208 Interme- diate Chemical structure number Compound name Starting materials Analytics/yield 201

6- (trifluoromethoxy)pyri- din-3-amine dihydrochloride; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆): δ ppm = 8.52 (d, J = 2.8 Hz, 1H), 8.13 (dd, J = 8.7, 2.7 Hz, 1H), 7.39-7.43 (m, 1H). 79% yield 202

6- (difluoromethoxy)pyri- din-3-amine; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.43 (d, J = 2.8 Hz, 1H), 8.05 (dd, J = 8.7, 2.7 Hz, 1H), 7.69 (t, J = 72.65 Hz, 1H), 7.20 (d, J = 8.9 Hz, 1H). 86% yield 203

6- (difluoromethyl)pyridin- 3-amine dihydrochloride; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1H NMR (400 MHz, DMSO-d6, 22° C.): Shift = 8.78 (d, J = 2.0 Hz, 1H), 8.06 (dd, J = 8.4, 2.3 Hz, 1H), 7.78 (d, J = 8.4 Hz, 1H), 7.00 (t, J = 54.8 Hz, 1H). 77% yield 204

4-fluoro-3- (trifluoromethoxy)ani- line; carbonothioyl dichlorode ¹H NMR (400 MHz, DMSO-d₆): δ ppm = 7.83-7.88 (m, 1H), 7.59- 7.65 (m, 2H). 53% yield 205

3-(difluoromethoxy)- 4-fluoroaniline hydrochloride; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.60 (dd, J = 7.0, 2.4 Hz, 1H), 7.51 (dd, J = 10.4, 8.9 Hz, 1H), 7.39-7.44 (m, 1H), 7.30 (t, J = 72.8 Hz, 1H). 73% yield 206

2-fluoro-4- (trifluoromethoxy)ani- line; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆): δ ppm = 7.70 (dd, J = 10.0, 2.4 Hz, 1H), 7.64 (t, J = 8.7 Hz, 1H), 7.33 (ddt, J = 8.9, 2.4, 1.3 Hz, 1H). 35% yield 207

4-(difluoromethoxy)- 2-fluoroaniline; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.58 (t, J = 8.9 Hz, 1H), 7.43 (dd, J = 11.0, 2.7 Hz, 1H), 7.34 (t, J = 73.3 Hz, 1H), 7.07- 7.13 (m, 1H). 70% yield 208

2-chlroo-4- (difluoromethoxy)aniline; carbonothioyl dichloride ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.64 (d, J = 8.9 Hz, 1H), 7.57 (d, J = 2.8 Hz, 1H), 7.34 (t, J = 73.3 Hz, 1H), 7.25 (dd, J = 8.9, 2.8 Hz, 1H). 48% yield

Intermediate 209 [4-amino-2-(4-fluoroanilino)thiazol-5-yl]-[4-(2-hydroxyethoxy)phenyl]methanone

The title compound (36 mg, 0.09 mmol, 11% yield) was isolated as a byproduct in the formation of 2-(4-{[4-amino-2-(4-fluoroanilino)-1,3-thiazol-5-yl]carbonyl}phenoxy)ethyl acetate (Intermediate 112).

LC-MS (method 2): Rt=0.90 min; MS(ESIpos) m/z=374.3 [M+H]⁺

Intermediate 210 Ethyl 4-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]benzoate

1-fluoro-4-isothiocyanatobenzene (5.65 g, 36.9 mmol) was dissolved in acetonitrile (200 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (5.61 g, 36.9 mmol) and cyanamide (1.86 g, 44.3 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (2.8 g, 18.5 mmol) and ethyl 4-(bromoacetyl)benzoate (10 g, 36.9 mmol) dissolved in acetonitrile (80 mL) were added. The reaction mixture was stirred at rt for 2.5 h and treated with water. The precipitate was filtered off, washed with water and dried in vacuo to give 14.2 g (quant.) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.34 (t, J=7.1 Hz, 3H), 434 (d, J=7.1 Hz, 3H), 7.22 (m, 2H), 7.61 (m, 1H), 7.78 (d, J=8.62 Hz, 2H). 8.04 (d, J=8.62 Hz, 2H), 8.32 (m, 2H), 10.87 (br s, 1H).

LC-MS (method 2): Rt=1.10 min; MS(ESIpos) m/z=386.3 [M+H]⁺

Intermediate 211 Rac-4-[(4-amino-2-{[(2RS)-1-amino-1-oxopropan-2-yl](4-fluorophenyl)amino}-1,3-thiazol-5-yl)carbonyl]benzoic Acid

rac-Ethyl 4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]benzoate (15.47 g, 33 mmol, Example 257) was suspended in THF (150 mL) and treated with 1 M aqueous sodium hydroxide (43 mL, 43 mmol). The reaction mixture was stirred overnight followed by the addition of 1 M aqueous hydrochloric acid up to pH 3. The solution was extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness to give 11.63 g (25 mmol, 87% yield) of the title compound.

LC-MS (method 2): Rt=0.53 min; MS(ESIpos) m/z=429.4 [M+H]⁺

Intermediate 212 Ethyl 4-[4-amino-2-(4-chloro-3-fluoro-anilino)thiazole-5-carbonyl]benzoate

1-chloro-2-fluoro-4-isothiocyanatobenzene (692 mg, 3.7 mmol) was dissolved in acetonitrile (20 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (561.5 mg, 3.68 mmol) and cyanamide (186 mg, 4.43 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (280.75 mg, 1.84 mmol) and ethyl 4-(bromoacetyl)benzoate (1 g, 3.7 mmol) dissolved in acetonitrile (14 mL) were added. The reaction mixture was stirred 2 h at rt. The suspension was treated with water and the precipitate was isolated by filtration, washed with water and some ethyl acetate and dried in vacuo to give 1.24 g (2.52 mmol, 68%, purity 85%) of the title compound.

LC-MS (method 2): Rt=1.12 min; MS(ESIpos) m/z=420.2 [M+H]⁺

Intermediate 213 Rac-4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-chloro-3-fluoro-anilino)thiazole-5-carbonyl]benzoic Acid

rac-Ethyl 4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-chloro-3-fluoro-anilino)thiazole-5-carbonyl]benzoate (830 mg, 1.7 mmol, Example 258) was suspended in THF (10 mL) and treated with 2 M aqueous sodium hydroxide (8.5 mL, 17 mmol). The reaction mixture was stirred at room temperature overnight followed by the addition of 2 M aqueous hydrochloric acid up to pH 3. The precipitate was filtered off, washed with water and dried in vacuo to yield 680 mg (1.47 mmol, 88%) of the title compound.

LC-MS (method 1): Rt=1.07 min; MS(ESIpos) m/z=463.1 [M+H]⁺

Intermediate 214 Ethyl 2-[4-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoate

1-fluoro-4-isothiocyanatobenzene (349 mg, 2.28 mmol) was dissolved in acetonitrile (8 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (346 mg, 2.28 mmol) and cyanamide (115 mg, 2.7 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (173 mg, 1.14 mmol) and ethyl 2-[4-(2-bromoacetyl)phenoxy]—2-methyl-propanoate (750 mg, 2.28 mmol) dissolved in acetonitrile (4 mL) were added. The reaction mixture was stirred overnight at rt. The suspension was treated with water and the precipitate was isolated by filtration, washed with water and dried in vacuo to give 1.01 g (2.28 mmol, 70%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.15 (t, J=7.1 Hz, 3H), 1.57 (s, 6H), 4.17 (q, J=7.1 Hz, 2H), 6.80 (m, 2H), 7.15 (m, 2H), 7.50 (m, 2H), 7.59 (m, 2H), 8.12 (m, 2H), 10.51 (m, 1H).

LC-MS (method 2): Rt=1.24 min; MS(ESIpos) m/z=444.3 [M+H]⁺

Intermediate 215 Rac-2-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoic Acid

Rac-Ethyl 2-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoate (925 mg, 1.79 mmol, Example 259) was suspended in THF (10 mL) and treated with 1 M aqueous sodium hydroxide (2.7 mL, 2.7 mmol). The reaction mixture was stirred at room temperature overnight followed by the addition of 1 M aqueous hydrochloric acid up to pH 3. The reaction mixture was extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness to yield 861 mg (1.6 mmol, 91%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.16 (d, J=7.35 Hz, 3H), 1.52 (s, 6H), 5.06 (m, 1H), 6.76 (m, 2H), 7.24 (s, 1H), 7.33 (t, J=8.74 Hz, 2H), 7.45 (m, 2H), 7.57 (s, 1H), 7.64 (m, 2H), 8.10 (m, 2H), 13.15 (in, 1H).

LC-MS (method 2): Rt=0.63 min; MS(ESIpos) m/z=487.4 [M+H]⁺

Intermediate 216 [4-amino-2-(4-fluoroanilino)thiazol-5-yl]-(6-amino-3-pyridyl)methanone

1-fluoro-4-isothiocyanatobenzene (250 mg, 1.63 mmol) was dissolved in acetonitrile (8 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (248.5 mg, 1.63 mmol) and cyanamide (82 mg, 1.96 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (124.2 mg, 0.82 mmol) and 1-(6-amino-3-pyridyl)-2-bromo-ethanone (351 mg, 1.63 mmol) dissolved in acetonitrile (7 mL) were added. The reaction mixture was stirred 2.5 h at rt. The suspension was treated with water and the precipitate was isolated by filtration, washed with water and dried in vacuo to give 350 mg (1.05 mmol, 64%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=4.65 (s, 2H), 6.96 (d, J=9.63 Hz, 1H), 7.03 (d, J=9.38 Hz, 2H), 8.24 (m, 3H), 8.62 (d, J=2.28 Hz, 2H), 8.82 (d, J=2.28 Hz, 1H).

LC-MS (method 2): Rt=0.82 min; MS(ESIpos) m/z=330.2 [M+H]⁺

Intermediate 217 Benzyl N-[5-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]-2-pyridyl]carbamate

[4-amino-2-(4-fluoroanilino)thiazol-5-yl]-(6-amino-3-pyridyl)methanone (205 mg, 0.62 mmol, Intermediate 216) was suspended in THF (5 mL) and treated with benzyl carbonochloridate (106 mg, 0.62 mmol) and triethylamine (94 mg, 0.93 mmol). The reaction mixture was stirred overnight at rt followed by the addition of further benzyl carbonochloridate (106 mg, 0.62 mmol), triethylamine (94 mg, 0.93 mmol) and DMAP (1 mg). After 4.5 h water was added and the reaction mixture was extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The residue was purified via Biotage chromatography (method Y) to yield 25 mg (0.05 mmol, 9%) of the title compound.

LC-MS (method 2): Rt=1.11 min; MS(ESIpos) m/z=464.3 [M+H]⁺

Intermediate 218 [4-amino-2-(4-fluoroanilino)-1,3-thiazol-5-yl](6-bromopyridin-3-yl)methanone

1-fluoro-4-isothiocyanatobenzene (1.29 g, 8.43 mmol) was dissolved in acetonitrile (65 mL) followed by the addition of 1,8-diazabicyclo(5.4.0)undec-7-ene (1.28 g, 8.43 mmol) and cyanamide (0.43 g, 10.1 mmol). After stirring for 45 min at rt, further 1,8-diazabicyclo(5.4.0)undec-7-ene (0.64 g, 4.2 mmol) and 2-bromo-1-(6-bromo-3-pyridyl)ethanone (2.35 g, 8.43 mmol) dissolved in acetonitrile (15 mL) were added. The reaction mixture was stirred at rt overnight and treated with water. The precipitate was filtered off, washed with water and dried in vacuo to give 2.52 g (6.4 mmol, 76%) of the title compound.

LC-MS (method 2): Rt=0.95 min; MS(ESIpos) m/z=395.1 [M+H]⁺

EXPERIMENTAL SECTION—PREPARATION OF EXAMPLE COMPOUNDS Example 1 Rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-4-methoxy-2-methyl-anilino)propanamide

[4-amino-2-(4-methoxy-2-methyl-anilino)thiazol-5-yl]-phenyl-methanone (100 mg, 0.295 mmol; Intermediate 4) were dissolved in N,N-dimethylformamide (3 mL) followed by the addition of potassium carbonate (407 mg, 2.95 mmol) and rac-2-bromopropanamide (224 mg, 1.47 mmol). The reaction mixture was stirred at 90° C. for 2 h. The reaction mixture was filtrated and purified by RIP-HPLC (method D, basic) to give 57 mg (47% yield) of the title compound.

¹H-NMR: (400 MHz, DMSO-d6): δ ppm=1.03 (d, J=7.35 Hz, 3H), 2.13 (s, 3H), 3.75-3.78 (m, 3H), 4.93-5.13 (m, 1H), 6.85-6.93 (m, 2H), 7.17-7.24 (m, 1H), 7.34-7.42 (m, 3H), 7.42-7.50 (m, 2H), 7.54 (br s, 1H), 7.64 (d, J=8.62 Hz, 1H), 7.73-8.60 (m, 2H).

LC-MS (method 2) Rt=1.08 min; MS (ESIpos): m/z=411.5 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 6, below, using the procedure as for Example 1.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) after precipitation, extraction or filtration of the reaction mixture if necessary.

Enantiomers were separated from their racemate by chiral HPLC using the column and solvent conditions stated.

TABLE 6 Examples 2-80 Example Chemical structure Starting number Compound name materials Analytics/purification/yield 2

Intermediate 5; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.14 (d, J = 7.35 Hz, 3 H), 2.93 (s, 6 H), 5.06 (br d, J = 6.08 Hz, 1 H), 6.70 (d, J = 9.38 Hz, 2 H), 7.15-72.0 (m, 1 H), 7.28 (d, J = 8.87 Hz, 2 H), 7.34- 7.41 (m, 3 H), 7.43-7.50 (m, 3 H), 7.74-8.53 (m, 2 H). LC-MS (method 2) Rt = 1.11 min; MS (ESIpos): m/z = 410.6 [M + H]⁺ RP-HPLC (method D, basic) 51% yield 3

Intermediate 6; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 1.27 (d, J = 6.08 Hz, 6 H), 4.62 (spt, J = 6.04 Hz, 1 H), 5.01- 5.11 (m, 1 H), 6.96 (d, J = 9.12 Hz, 2 H), 7.21 (s, 1 H), 7.35- 7.49 (m, 7 H), 7.52 (s, 1 H), 7.75- 8.51 (m, 2 H). LC-MS (method 1) Rt = 1.18 min; MS (ESIpos): m/z = 425.7 [M + H]⁺ RT-HPLC (method D, basic) 42% yield 4

Intermediate 7; rac-2- bromopropan- amide ¹H-NMR (500 MHz, DMSO-d6): δ ppm = 1.32 (d, J = 6.99 Hz, 3 H), 4.95 (q, J = 6.57 Hz, 1 H), 7.04-7.11 (m, 1 H), 7.33 (br s, 2 H), 7.39-7.48 (m, 4 H), 7.56 (dd, J = 7.79, 1.75 Hz, 2 H), 7.96 (br s, 2 H) LC-MS (method 2) Rt = 1.08 min; MS (ESIpos): m/z = 421.5 [M + H]⁺ RP-HPLC (method D, basic) preparative flash chromatography (method X, 40- 100%) 33% yield 5

Intermediate 8; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.03-1.15 (m, 3 H), 4.98-5.13 (m, 1 H), 7.21-7.34 (m, 1 H), 7.34-7.55 (m, 6 H), 7.57-7.87 (m, 2 H), 7.90-8.49 (m, 3 H). LC-MS (method 2) Rt = 1.1 min; MS (ESIpos): m/z = 463.2 [M + H]⁺ RT-HPLC (method D, basic) 83% yield 6

Intermediate 9; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 2.45 (s, 3 H), 5.00-5.11 (m, 1 H), 7.24-7.28 (m, 2 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.59 (s, 1 H), 7.62-7.67 (m, 2 H), 7.74 (dd, J = 8.11, 2.28 Hz, 1 H), 7.97- 8.46 (m, 2 H), 8.54 (d, J = 2.03 Hz, 1 H). LC-MS (method 2): Rt = 0.93 min; MS (ESIpos): m/z = 400.4 [M + H]⁺ RT-HPLC (method C, basic) 59% yield 7

Intermediate 10; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 5.06 (br d, J = 7.10 Hz, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.38-7.42 (m, 2 H), 7.59 (s, 1 H), 7.61-7.67 (m, 2 H), 8.10-8.50 (m, 2 H), 8.59-8.64 (m, 2 H). LC-MS (method 2) Rt = 0.85 min; MS (ESIpos): m/z = 386.3 [M + H]⁺ RT-HPLC (method C, acidic) 17% yield 8

Intermediate 11; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.14 (d, J = 7.35 Hz, 3 H), 4.89-5.13 (m, 1 H), 7.15- 7.34 (m, 7 H), 7.40 (tdd, J = 7.73, 7.73, 5.58, 1.77 Hz, 1 H), 7.53- 7.66 (m, 3 H), 8.09 (br s, 2 H), LC-MS (method 1) Rt = 1.05 min; MS (ESIpos): m/z = 403.4 [M + H]⁺ RT-HPLC (method D, basic) 71% yield 9

Intermediate 12; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.69 Hz, 1 H), 7.31 (s, 1 H), 7.36-7.43 (m, 3 H), 7.48-7.52 (m, 2 H), 7.64 (s, 1 H), 7.81 (d, J = 8.62 Hz, 2 H), 8.16 (br s, 2 H), 7.99 (d, J = 8.36 Hz, 2 H). LC-MS (method 1) Rt = 0.99 min; MS (ESIpos): m/z = 392.3 [M + H]⁺ RP-HPLC (method C, basic) 8% yield 10

Intermediate 13; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.01-5.14 (m, 1 H), 7.27 (s, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.59 (s, 1 H), 7.62-7.67 (m, 2 H), 7.77 (s, 2 H), 7.89-7.91 (m, 1 H), 8.08-8.64 (m, 2 H). LC-MS (method 2) Rt = 1.30 min; MS (ESIpos): m/z = 487.2 [M + H]⁺ RP-HPLC (method D, basic) 22% yield 11

Intermediate 14; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.09 (d, J = 7.35 Hz, 3 H), 4.98-5.21 (m, 1 H), 7.26- 7.34 (m, 1 H), 7.36-7.55 (m, 6 H), 7.59-7.76 (m, 2 H), 7.89- 8.45 (m, 2 H), 8.03 (dd, J = 8.87, 5.83 Hz, 1 H). LC-MS (method 2) Rt = 1.13 min; MS (ESIpos): m/z = 419.4 [M + H]⁺ RP-HPLC (method D, basic) 48% yield 12

Intermediate 15; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.01-5.10 (m, 1 H), 7.24- 7.28 (m, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.57-7.66 (m, 4 H), 7.78 (dt, J = 7.79, 1.30 Hz, 1 H), 7.86-7.89 (m, 2 H), 8.25 (br s, 2 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z = 410.3 [M + H]⁺ RP-HPLC (method C, basic) 31% yield 13

Intermediate 16; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 2.77 (s, 6 H), 4.99-5.09 (m, 1 H), 7.18 (d, J = 8.62 Hz, 1 H), 7.22-7.27 (m, 1 H), 7.35-7.46 (m, 4 H), 7.47-7.52 (m, 2 H), 7.57 (s, 1 H), 7.63 (d, J = 2.28 Hz, 1 H), 8.16 (br s, 2 H). LC-MS (method 2) Rt = 0.91 min; MS (ESIpos): m/z = 444.5 [M + H]⁺ RP-HPLC (method C, basic) 51% yield 14

Intermediate 17; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 3.85 (s, 3 H), 5.01-5.11 (m, 1 H), 6.83 (dd, J = 8.62, 0.51 Hz, 1 H), 7.24-7.28 (m, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.59 (s, 1 H), 7.62-7.68 (m, 2 H), 7.82 (dd, J = 8.62, 2.53 Hz, 1 H), 8.17 (br s, 2 H), 8.33 (d, J = 2.03 Hz, 1 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z = 416.4 [M + H]⁺ RP-HPLC (method C, basic) 43% yield 15

Intermediate 18; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 2.75 (d, J = 4.56 Hz, 3 H), 4.99-5.12 (m, 1 H), 7.25 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.53 (d, J = 8.36 Hz, 2 H), 7.58 (br s, 1 H), 7.60-7.66 (m, 2 H), 7.78 (d, J = 8.36 Hz, 2 H), 8.17 (br s, 2 H), 8.46 (q, J = 4.48 Hz, 1 H). LC-MS (method 2) Rt = 0.85 min; MS (ESIpos): m/z = 442.5 [M + H]⁺ RP-HPLC (method C, basic) 10% yield 16

Intermediate 19; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 4.98-5.17 (m, 1 H), 7.22- 7.29 (m, 3 H), 7.29-7.36 (m, 3 H), 7.39-7.48 (m, 1 H), 7.58 (s, 1 H), 7.64 (dd, J = 8.62, 5.07 Hz, 2 H), 7.97-8.59 (m, 2 H). LC-MS (method 1) Rt = 1.10 min; MS (ESIpos): m/z = 403.3 [M + H]⁺ RP-HPLC (method D, basic) 68% yield 17

Intermediate 20; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d₆): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 3.89 (s, 3 H), 4.96-5.10 (m, 1 H), 7.22 (d, J = 8.87 Hz, 1 H), 7.25 (s, 1 H), 7.35-7.43 (m, 3 H), 7.46-7.54 (m, 3 H), 7.59 (s, 1 H), 7.71 (d, J = 2.53 Hz, 1 H), 7.87-8.37 (m, 2 H). LC-MS (method 2) Rt = 1.10 min; MS (ESIpos): m/z = 431.3 [M + H]⁺ RP-HPLC (method D, basic) 60% yield 18

Intermediate 21; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.14 (d, J = 7.35 Hz, 3 H), 3.78 (s, 3 H), 5.01-5.10 (m, 1 H), 7.01 (d, J = 9.13 Hz, 2 H), 7.17-7.24 (m, 3 H), 7.46 (d, J = 8.62 Hz, 2 H), 7.51-7.58 (m, 3 H), 8.09 (br s, 2 H). LC-MS (method 1) Rt = 1.08 min; MS (ESIpos): m/z = 415.6 [M + H]⁺ RT-HPLC (method C, acidic) 36% yield 19

Intermediate 22; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 3.23 (s, 3 H), 4.96-5.15 (m, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.59 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.70 (d, J = 8.36 Hz, 2 H), 7.93 (d, J = 8.62 Hz, 2 H), 8.04-8.56 (m, 2 H). LC-MS (method 1) Rt = 0.91 min; MS (ESIpos): m/z = 463.4 [M + H]⁺ RP-HPLC (method C, basic) 31% yield 20

Intermediate 23; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.00-5.13 (m, 1 H), 7.10 (s, 1 H), 7.26 (s, 1 H), 7.34 (t, J = 8.74 Hz, 2 H), 7.56-7.73 (m, 7 H), 7.78 (t, J = 1.27 Hz, 1 H), 7.88-8.62 (m, 2 H), 8.30 (s, 1 H). LC-MS (method 1) Rt = 0.70 min; MS (ESIpos): m/z = 451.5 [M + H]⁺ RP-HPLC (method C, basic) 68% yield 21

Intermediate 24; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 5.01-5.11 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.45 (dd, J = 7.86, 1.27 Hz, 1 H), 7.55 (dd, J = 9.89, 1.27 Hz, 1 H), 7.58-7.66 (m, 3 H), 7.93 (dd, J = 7.86, 6.59 Hz, 1 H), 8.11- 8.52 (m, 2 H). LC-MS (method 1) Rt = 1.08 min; MS (ESIpos): m/z = 428.3 [M + H]⁺ RP-HPLC (method C, basic) 5% yield 21.1 and (R)-2-(N-[4-amino-5-(4-cyano-3- 21.2 fluoro-benzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4-cyano-3- fluoro-benzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide 21.1

Example 21 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 5.01-5.11 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.45 (dd, J = 7.86, 1.27 Hz, 1 H), 7.55 (dd, J = 9.89, 1.27 Hz, 1 H), 7.58-7.66 (m, 3 H), 7.93 (dd, J = 7.86, 6.59 Hz, 1 H), 8.11- 8.52 (m, 2 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 428.3 [M + H]⁺ RP-HPLC (method C, basic) 8% Chiral HPLC Example 21.1 HPLC separation of rac-2-(N-[4-amino-5-(4-cyano-3-fluoro-benzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (Example 21, 106 mg. 0.25 mmol) on a chiral column followed by another preparative HPLC gave 9.2 mg (8% yield) of 2-(N-[4-amino-5-(4-cyano-3-fluoro- benzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamino; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow; 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.46 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamino; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 21.2

Example 21 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 5.01-5.11 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.45 (dd, J = 7.86, 1.27 Hz, 1 H), 7.55 (dd, J = 9.89, 1.27 Hz, 1 H), 7.58-7.66 (m, 3 H), 7.93 (dd, J = 7.86, 6.59 Hz, 1 H), 8.11- 8.52 (m, 2 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 428.3 [M + H]⁺ RP-HPLC (method C, basic) 7% Chiral HPLC Example 21.2 HPLC separation of rac-2-(N-[4-amino-5-(4-cyano-3-fluoro-benzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (Example 21, 106 mg, 0.25 mmol) on a chiral column followed by another preparative HPLC gave 7 mg (6% yield) of 2-(N-[4-amino-5-(4-cyano-3-fluoro-benzoyl)thiazol- 2-yl]-4-fluoro-anilino)propanamide, enantiomer 2 Preparative chiral HPLC Instrument: PreCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow; 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 7.71 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 22

Intermediate 25: rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.01-5.11 (m, 1 H), 7.27 (s, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.45 (dd, J = 8.11, 1.27 Hz, 1 H), 7.56 (dd, J = 9.76, 1.14 Hz, 1 H), 7.59 (s, 1 H), 7.64 (dd, J = 8.74, 4.94 Hz, 2 H), 7.92-7.97 (m, 1 H), 8.18-8.50 (m, 2 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 428.3 [M + H]⁺ RP-HPLC (method D, basic) 40% yield 23

Intermediate 26; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.14 (d, J = 7.60 Hz, 3 H), 3.75 (s, 3 H), 4.98-5.09 (m, 1 H), 6.76 (d, J = 20.28 Hz, 1 H), 6.78 (br d, J = 23.32 Hz, 1 H), 7.22-7.27 (m, 2 H), 7.31 (t, J = 8.87 Hz, 2 H), 7.57 (s, 1 H), 7.59-7.64 (m, 2 H), 8.03 (br s, 2 H). LC-MS (method 2) Rt = 1.06 min; MS (ESIpos): m/z = 433.4 [M + H]⁺ RP-HPLC (method D, basic) 47% yield 24

Intermediate 27; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.28 (s, 1 H), 7.36-7.42 (m, 3 H), 7.45-7.52 (m, 4 H), 7.61 (s, 1 H), 7.69-7.75 (m, 2 H), 8.16 (br s, 2 H). LC-MS (method 2) Rt = 1.20 min; MS (ESIpos): m/z = 451.5 [M + H]⁺ RP-HPLC (method D, basic) 49% yield 25

Intermediate 28; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.02-5.10 (m, 1 H), 7.26 (s, 1 H), 7.34 (t, J = 8.62 Hz, 3H), 7.41-7.49 (m, 1 H), 7.52 (ddd, J = 11.15, 7.98, 2.15 Hz, 1 H), 7.58 (s, 1 H), 7.62-7.66 (m, 2 H), 7.98-8.50 (m, 2 H). LC-MS (method 2) Rt = 1.14 min; MS (ESIpos): m/z = 419.3 [M + H]⁺ RP-HPLC (method D, basic) 30% yield 26

Intermediate 29. rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.00-5.11 (m, 1 H), 7.26 (s, 1 H), 7.34 (t, J = 8.74 Hz, 2 H), 7.44 (dd, J = 8.36, 2.03 Hz, 1 H), 7.5-7.61 (m, 1 H), 7.62-7.66 (m, 2 H), 7.67 (d, J = 11.66 Hz, 1 H), 7.67 (d, J = 1.27 Hz, 1 H), 8.03-8.48 (m, 2 H). LC-MS (method 2) Rt = 1.24 min; MS (ESIpos): m/z = 453.1 [M + H]+ RP-HPLC (method D, basic) 38% yield 27

Intermediate 30; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15-1.19 (m, 3 H), 5.02-5.09 (m, 1 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.28 (s, 1 H), 7.50 (d, J = 8.11 Hz, 2 H), 7.56 (dd, J = 8.62, 5.58 Hz, 2 H), 7.61 (s, 1 H), 7.73 (d, J = 9.13 Hz, 2 H), 7.90-8.49 (m, 2 H). LC-MS (method 2) Rt = 1.23 min; MS (ESIpos): m/z = 469.3 [M + H]⁺ 59% yield 27.1 and (R)-2-[N-[4-amino-5-(4- 27.2 fluorobenozyl)thiazol-2-yl]-4- (trifluoromethoxy)anilino]propan- amide and (S)-2-[N-[4-amino-5-(4- fluorobenzoyl)thiazol-2-yl]-4- (trifluoromethoxy)anilino]propan- amide 27.1

Example 27 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.27 Hz, 1 H), 7.22 (t, J = 9.00 Hz, 2 H), 7.28 (s, 1 H), 7.48-7.53 (m, 2 H), 7.53- 7.59 (m, 2 H), 7.61 (s, 1 H), 7.70- 7.75 (m, 2 H), 7.95-8.42 (m, 2 H). LC-MS (method 1) Rt = 1.23 min; MS (ESIpos): m/z = 469.3 [M + H]⁺ 39% yield Chiral HPLC Example 27.1 HPLC separation of rac-2-[N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4- (trifluoromethoxy)anilino]propanamide (1130 mg, 2.4 mmol; Example 27) on a chiral column gave 448 mg (39% yield) of 2-[N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4- (trifluoromethoxy)anilino]propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.99 min Instrument: Waters Allicance 2695; Column: YMC Cellulose SB 3μm, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 27.2

Example 27 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.60 Hz, 3 H), 5.05 (br d, J = 7.10 Hz, 1 H), 7.19-7.25 (m, 2 H), 7.28 (s, 1 H), 7.47-7.53 (m, 2 H), 7.53- 7.59 (m, 2 H), 7.59-7.63 (m, 1 H), 7.70-7.76 (m, 2 H), 7.96- 8.40 (m, 2 H). LC-MS (method 1) Rt = 1.23 min; MS (ESIpos): m/z = 469.2 [M + H]⁺ 37% yield Chiral HPLC Example 27.2 HPLC separation of rac-2-[N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4- (trifluoromethoxy)anilino]propanamide (1130 mg, 2.4 mmol; Example 27) on a chiral column gave 430 mg (37% yield) of 2-[N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4- (trifluoromethoxy)anilino]propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.81 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 28

Intermediate 31; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.52 Hz, 1 H), 7.30 (s, 1 H), 7.36-7.44 (m, 3 H), 7.47-7.52 (m, 2 H), 7.63 (s, 1 H), 7.80-7.91 (m, 4 H), 8.18 (br s, 2 H). LC-MS (method 1) Rt = 1.18 min; MS (ESIpos): m/z = 435.3 [M + H]⁺ RP-HPLC (method D, basic) 29% yield 29

Intermediate 32; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 5.02-5.13 (m, 1 H), 7.25 (s, 1 H), 7.30-7.38 (m, 2 H), 7.60 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.94 (d, J = 7.86 Hz, 1 H), 8.12 (dd, J = 8.11, 1.77 Hz, 1 H), 8.23-8.46 (m, 2 H), 8.84 (br d, J = 1.80 Hz, 1 H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 454.5 [M + H]+ RP-HPLC (method C, basic) 42% yield 29.1 and (R)-2-(N-[4-amino-5-[6- 29.2 (trifluoromethyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide and (S)-2-(N-[4-amino-5-[6- (trifluoromethyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 29.1

Example 29 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.00-5.16 (m, 1 H), 7.27 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.58-7.69 (m, 3 H), 7.93 (d, J = 8.11 Hz, 1 H), 8.11 (br d, J = 1.77 Hz, 1 H), 8.17-8.50 (m, 2 H), 8.84 (d, J = 1.27 Hz, 1 H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 454.5 [M + H]⁺ [α]_(D) ²⁰ = +53.2° c = 1.00, dimethylsulfoxide) 13% yield Chiral HPLC Example 29.1 HPLC separation of rac-2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]-4- fluoro-anilino)propanamide (394 mg, 1.0 mmol; Example 29) on a chiral column gave 163 mg (33% yield) of 2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.24 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 29.2

Example 29 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.01-5.17 (m, 1 H), 7.28 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.54-7.72 (m, 3 H), 7.93 (d, J = 8.11 Hz, 1 H), 8.12 (dd, J = 8.11, 1.77 Hz, 1 H), 8.21- 8.54 (m, 2 H), 8.84 (d, J = 1.27 Hz, 1 H). LC-MS (method 1) Rt = 1.12 min; MS (ESIpos): m/z = 454.5 [M + H]⁺ [α]_(D) ²⁰ = −44.3° (c = 1.00, dimethylsulfoxide) 13% yield Chiral HPLC Example 29.2 HPLC separation of rac-2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]-4- fluoro-anilino)propanamide (394 mg, 1.0 mmol; Example 29) on a chiral column gave 164 mg (33% yield) of 2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic; 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.26 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine, eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 30

Intermediate 33: rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.11-1.22 (m, 3 H), 4.92-5.22 (m, 1 H), 7.28-7.36 (m, 1 H), 7.39-7.54 (m, 6 H), 7.59-7.76 (m, 2 H), 7.79-7.93 (m, 1 H), 7.97-8.32 (m, 2 H). LC-MS (method 1) Rt = 1.16 min; MS (ESIpos): m/z = 417.2 [M + H]⁺ RP-HPLC (method D, basic) 37% yield 31

Intermediate 34; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 1.98 (quin, J = 7.41 Hz, 2 H), 2.82 (td, J = 7.29, 4.18 Hz, 4 H), 5.04 (br q, J = 6.67 Hz, 1 H), 7.18- 7.23 (m, 2 H), 7.24 (s, 1 H), 7.29-7.35 (m, 3 H), 7.56-7.59 (m, 1 H), 7.60-7.65 (m, 2 H), 7.80-8.40 (m, 2 H). LC-MS (method 2) Rt = 1.23 min; MS (ESIpos): m/z = 425.6 [M + H]⁺ RP-HPLC (method D, basic) 24% yield 32

Intermediate 35; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.02 (q, J = 7.69 Hz, 1 H), 7.29 (s, 1 H), 7.36-7.44 (m, 3 H), 7.47-7.53 (m, 3 H), 7.54- 7.60 (m, 1 H), 7.61-7.65 (m, 1 H), 7.77 (ddd, J = 11.47, 7.54, 2.28 Hz, 1 H), 8.15 (br s, 2 H). LC-MS (method 1) Rt = 1.11 min; MS (ESIpos): m/z = 403.3 [M + H]⁺ RP-HPLC (method C, basic) 48% yield 33

Intermediate 36; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 4.90-5.24 (m, 1 H), 7.23- 7.29 (m, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.59 (s, 1 H), 7.60- 7.66 (m, 4 H), 7.86 (d, J = 8.36 Hz, 2 H), 8.05-8.57 (m, 2 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z = 410.3 [M + H]⁺ RP-HPLC (method C, basc) 78% yield 33.1 and (R)-2-(N-[4-amino-5-(4- 33.2 cyanobenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- cyanobenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide 33.1

Example 33 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.02-5.11 (m, 1 H), 7.24- 7.28 (m, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.58 (s, 1 H), 7.61- 7.66 (m, 4 H), 7.86 (d, J = 8.62 Hz, 2 H), 8.05-8.50 (m, 2 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z = 410.3 [M + H]⁺ [α]_(D) ²⁰ = +93.9° (c = 1.00, dimethylsulfoxide) 31% yield Chiral HPLC Example 33.1 HPLC separation of rac-2-(N-[4-amino-5-(4-cyanobenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (96 mg, 0.23 mmol; Example 33) on a chiral column gave 30 mg (30% yield) of 2-(N-[4-amino-5-(4-cyanobenozyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.26 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 33.2

Example 33 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 5.01-5.12 (m, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.58 (s, 1 H), 7.63 (d, J = 8.36 Hz, 4 H), 7.86 (d, J = 8.36 Hz, 2 H), 8.05-8.53 (m, 2 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z = 410.3 [M + H]⁺ [α]_(D) ²⁰ = −89.9° (c = 1.00, dimethylsulfoxide) 30% yield Chiral HPLC Example 33.2 HPLC separation of rac-2-(N-[4-amino-5-(4-cyanobenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (96 mg, 0.23 mmol; Example 33) on a chiral column gave 32 mg (31% yield) of 2-(N-[4-amino-5-(4-cyanobenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.05 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 34

Intermediate 37; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.26 (s, 1 H), 7.35-7.42 (m, 3 H), 7.47-7.50 (m, 2 H), 7.54- 7.63 (m, 5 H), 8.16 (br s, 2 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 402.2 [M + H]⁺ RT-HPLC (method D, basic) 9% yield 35

Intermediate 38; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 5.01 (q, J = 7.18 Hz, 1 H), 7.31 (s, 1 H), 7.37-7.45 (m, 3 H), 7.49-7.54 (m, 2 H), 7.62 (dd, J = 8.90, 2.53 Hz, 1 H), 7.64 (s, 1 H), 7.79 (d, J = 8.36 Hz, 1 H), 7.95 (d, J = 2.28 Hz, 1 H), 8.24 (br s, 2 H). LC-MS (method 1) Rt = 1.23 min; MS (ESIneg): m/z = 433.4 [M − H]⁺ RP-HPLC (method D, basic) 27% yield 36

Intermediate 39; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 5.02 (q, J = 7.35 Hz, 1 H), 7.30 (s, 1 H), 7.38-7.45 (m, 3 H), 7.49-7.53 (m, 3 H), 7.63 (s, 1 H), 7.72-7.77 (m, 2 H), 8.15 (br s, 2 H). LC-MS (method 1) Rt = 1.17 min; MS (ESIpos): m/z = 419.3 [M + H]⁺ RP-HPLC (method D, basic) 39% yield 37

Intermediate 40; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 5.02-5.11 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.43-7.52 (m, 4 H), 7.58 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.98-8.43 (m, 2 H). LC-MS (method 2) Rt = 1.18 min; MS (ESIpos): m/z = 419.2 [M + H]⁺ 55% yield 37.1 and (R)-2-(N-[4-amino-5-(4- 37.2 fluorobenzoyl)thiazol-2-yl]-4- chloro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- fluorobenzoyl)thiazol-2-yl]-4- chloro-anilino)propanamide 37.1

Example 37 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.13-1.19 (m, 3 H), 5.01-5.11 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.43- 7.53 (m, 4 H), 7.58 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.88-8.52 (m, 2 H). LC-MS (method 1) Rt = 1.19 min; MS (ESIpos): m/z = 419.2 [M + H]⁺ 31% yield Chiral HPLC Example 37.1 HPLC separation of rac-2-(N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4-chloro- anilino)propanamide (170 mg, 0.41 mmol, Example 37) on a chiral column gave 54 mg (31% yield) of 2-(N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic 60% A + 40% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.66 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethyalmine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 37.2

Example 37 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.01-5.11 (m, 1 H), 7.23- 7.27 (m, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.43-7.53 (m, 4 H), 7.58 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.92-8.37 (m, 2 H). LC-MS (method 1) Rt = 1.19 min; MS (ESIpos): m/z = 419.2 [M + H]⁺ 32% yield Chiral HPLC Example 37.2 HPLC separation of rac-2-(N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4-chloro- anilino)propanamide (170 mg, 0.41 mmol, Example 37) on a chiral column gave 57 mg (32% yield) 2-(N-[4-amino-5-(4-fluorobenzoyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.20 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 38

Intermediate 41; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 5.01-5.12 (m, 1 H), 7.25 (s, 1 H), 7.29-7.37 (m, 2 H), 7.43- 7.53 (m, 4 H), 7.58 (s, 1H), 7.63 (dd, J = 8.87, 5.07 Hz; 2 H), 7.92- 8.44 (m, 2 H). LC-MS (method 1) Rt = 1.19 min; MS (ESIpos): m/z = 419.2 [M + H]⁺ RT-HPLC (method C, basic) 42% yield 38.1 and (R)-2-(N-[4-amino-5-(4- 38.2 chlorobenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- chlorobenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide 38.1

Example 38 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 4.98-5.16 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.43-7.52 (m, 4 H), 7.58 (s, 1 H), 7.63 (dd, J = 8.87, 5.07 Hz, 2 H), 7.92-8.50 (m, 2 H). LC-MS (method 1) Rt = 1.19 min; MS (ESIpos): m/z = 419.2 [M + H]⁺ RP-HPLC (method D, basic) 16% yield Chiral HPLC Example 38.1 HPLC separation of rac-2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (115 mg, 0.27 mmol; Example 38) on a chiral column, followed by an additional RP-HPLC gave 19 mg (16% yield) of 2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]- 4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.23 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 38.2

Example 38 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.02-5.10 (m, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.43-7.52 (m, 4 H), 7.57-7.60 (m, 1 H), 7.63 (dd, J = 8.87, 5.07 Hz, 2 H), 7.95-8.47 (m, 2 H). LC-MS (method 1) Rt = 1.19 min; MS (ESIpos): m/z = 419.2 [M + H]⁺ RP-HPLC (method D, basic) 38% yield Chiral HPLC Example 38.2 HPLC separation of rac-2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (115 mg, 0.27 mmol; Example 38) on a chiral column, followed by an additional RP-HPLC gave 20 mg (17% yield) of 2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]- 4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.79 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic 60% A + 40% B; flow 1.4 mL/min; temperature: 25° C.; UV: 254 nm 39

Intermediate 42; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.07 (t, J = 7.10 Hz, 3 H), 1.15 (d, J = 7.35 Hz, 3 H), 1.53 (s, 6H), 4.12 (q, J = 7.01 Hz, 2 H), 5.02-5.09 (m, 1 H), 6.72-6.76 (m, 2 H), 7.24 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.44 (d, J = 8.87 Hz, 2 H), 7.58 (s, 1 H), 7.63 (dd, J = 9.00, 5.20 Hz, 2 H), 7.88 (br s, 2 H). LC-MS (method 1) Rt = 1.21 min; MS (ESIpos): m/z = 515.4 [M + H]⁺ RP-HPLC (method D, basic) 50% yield 40

Intermediate 43; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (br d, J = 6.84 Hz, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.38 (d, J = 7.86 Hz, 2 H), 7.58 (s, 1 H), 7.60-7.67 (m, 4 H), 7.95-8.56 (m, 2 H). LC-MS (method 1): Rt = 1.25 min; MS (ESIpos): m/z = 469.5 [M + H]⁺ RP-HPLC (method D, basic) 83% yield 40.1 and (R)-2-(N-[4-amino-5-[4- 40.2 (trifluoromethoxy)benzoyl)thiazol- 2-yl]-4-fluoro- anilino)propanamide and (S)-2-(N-[4-amino-5-[4- (trifluoromethoxy)benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide 40.1

Example 40 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.02-5.11 (m, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.38 (d, J = 8.11 Hz, 2 H), 7.58 (s, 1 H), 7.60-7.67 (m, 4 H), 7.95-8.46 (m, 2 H). LC-MS (method 2): Rt = 1.24 min; MS (ESIpos): m/z = 469.4 [M + H]+ 20% yield Chiral HPLC Example 40.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(trifluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (69 mg, 0.15 mmol; Example 40) on a chiral column gave 20 mg (20% yield) of 2-(N-[4-amino-5-[4-(trifluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IC 5μ 250 × 30 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 15% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar, UV: 254 nm Analytical chiral HPLC: Rt = 2.73 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IC 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 15% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 40.2

Example 40 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.02-5.11 (m, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.38 (d, J = 8.11 Hz, 2 H), 7.58 (s, 1 H), 7.60-7.67 (m, 4 H), 7.95-8.48 (m, 2 H). LC-MS (method 2) Rt = 1.25 min; MS (ESIpos): m/z = 469.3 [M + H]+ 21% yield. Chiral HPLC Example 40.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(trifluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (69 mg, 0.15 mmol; Example 40) on a chiral column gave 20 mg (21% yield) of 2-(N-[4-amino-5-[4-(trifluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IC 5μ 250 × 30 mm; eluent A: CO₂: eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 15% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar, UV: 254 nm Analytical chiral HPLC: Rt = 3.48 min Instrument: Agilent: 1260, Aurora SFC-Module; Column: Chiralpak IC 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 15% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 41

Intermediate 44; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.27 Hz, 1 H), 7.23-7.28 (m, 1 H), 7.44-7.53 (m, 4 H), 7.55-7.63 (m, 5 H), 7.96-8.44 (m, 2 H). LC-MS (method 2) Rt = 1.27 min; MS (ESIpos): m/z = 435.2 [M + H]⁺ 47% yield 41.1 and (R)-2-(N-[4-amino-5-(4- 41.2 chlorobenzoyl)thiazol-2-yl]-4- chloro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- chlorobenzoyl)thiazol-2-yl]-4- chloro-anilino)propanamide 41.1

Example 41 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.14-1.19 (m, 3 H), 5.06 (q, J = 7.10 Hz, 1 H), 7.27 (s, 1 H), 7.44-7.53 (m, 4 H), 7.55-7.64 (m, 5 H), 7.99-8.41 (m, 2 H). LC-MS (method 1) Rt = 1.27 min; MS (ESIpos): m/z = 435.2 [M + H]⁺ [α]_(D) ²⁰ = +83.8° (c = 1.00, dimethylsulfoxide) 25% yield Chiral HPLC Example 41.1 HPLC separation of rac-2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]-4-chloro- anilino)propanamide (144 mg, 0.33 mmol; Example 41) on a chiral column gave 36 mg (25% yield) of 2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.84 min Instrument: Waters Alliance 2695; Column: YMC Cellulose B 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 41.2

Example 41 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.10 Hz, 1 H), 7.26-7.29 (m, 1 H), 7.44-7.52 (m, 4 H), 7.55-7.63 (m, 5 H), 7.94-8.68 (m, 2 H). LC-MS (method 1) Rt = 1.27 min; MS (ESIpos): m/z = 435.2 [M + H]⁺ [α]_(D) ²⁰ = −78.4° (c = 1.00, dimethylsulfoxide) 18% yield Chiral HPLC Example 41.2 HPLC separation of rac-2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]-4-chloro- anilino)propanamide (144 mg, 0.33 mmol; Example 41) on a chiral column gave 26 mg (18% yield) of 2-(N-[4-amino-5-(4-chlorobenzoyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 25 × 50; eluent A: hexane; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.34 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A; hexane + 0.1 vol % diethylamine; eluent B; ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 42

Intermediate 45; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.00-5.13 (m, 1 H), 7.26 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.59 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.72 (dd, J = 37.13, 8.24 Hz, 4 H), 7.98- 8.51 (m, 2 H). LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 453.4 [M + H]⁺ RP-HPLC (method D, basic) 71% yield 43

Intermediate 46; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.07 (q, J = 7.27 Hz, 1 H), 7.28 (t, J = 72.24 Hz, 1 H), 7.18 (d, J = 9.38 Hz, 2 H), 7.30 (s, 1 H), 7.57 (d, J = 8.87 Hz, 2 H), 7.64 (s, 1 H), 7.86 (dd, J = 27.88, 8.36 Hz, 4 H), 8.18 (br s, 2 H), LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 501.4 [M + H]⁺ RP-HPLC (method D, basic) 54% yield 43.1 and (R)-2-[N-[4-amino-5-[4- 43.2 (difluoromethoxy)benzoyl]thiazol- 2-yl]-4- (trifluoromethyl)anilino]propana- mide and (S)-2-[N-[4-amino-5-[4- (difluoromethoxy)benzoyl]thiazol- 2-yl]-4- (trifluoromethyl)anilino]propana- mide 43.1

Example 43 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.07 (q, J = 7.35 Hz, 1 H), 7.28 (t, J = 74.01 Hz, 1 H), 7.18 (d, J = 8.62 Hz, 2 H), 7.31 (s, 1 H), 7.57 (d, J = 8.87 Hz, 2 H), 7.63 (s, 1 H), 7.86 (dd, J = 27.88, 8.62 Hz, 4 H), 7.99-8.36 (m, 2 H). LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 501.4 [M + H]⁺ 34% yield Chiral HPLC Example 43.1 HPLC separation of rac-2-[N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4- (trifluoromethyl)anilino]propanamide (280 mg, 0.56 mmol; Example 43) on a chiral column gave 99 mg (35% yield) of 2-[N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4- (trifluoromethyl)anilino]propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.50 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 43.2

Example 43 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.07 (q, J = 7.35 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.18 (d, J = 8.62 Hz, 2 H), 7.31 (s, 1 H), 7.57 (d, J = 8.87 Hz, 2 H), 7.63 (s, 1 H), 7.86 (dd, J = 27.88, 8.62 Hz, 4 H), 8.18 (br s, 2 H). LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 501.3 [M + H]⁺ 27% yield Chiral HPLC Example 43.2 HPLC separation of rac-2-[N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4- (trifluoromethyl)anilino]propanamide (280 mg, 0.56 mmol; Example 43) on a chiral column gave 79 mg (27% yield) of 2-[N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4- (trifluoromethyl)anilino]propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.44 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B; ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 44

Intermediate 47; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.10 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.17 (d, J = 8.62 Hz, 2 H), 7.25 (br s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (d, J = 8.87 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 8.21 (br s, 2 H). LC-MS (method 2) Rt = 1.13 min; MS (ESIpos): m/z = 451.6 [M + H]⁺ RP-HPLC (method D, basic) 87% yield 44.1 and (R)-2-(N-[4-amino-5-[4- 44.2 (difluoromethoxy)benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide and (S)-2-(N-[4-amino-5-[4- (difluoromethoxy)benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide 44.1

Example 44 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.02-5.10 (m, 1 H), 7.26 (t, J = 74.01 Hz, 1 H), 7.17 (d, J = 9.38 Hz, 2 H), 7.25 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.55 (d, J = 8.87 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.86-8.45 (m, 2 H). LC-MS (method 2) Rt = 1.12 min; MS (ESIpos): m/z = 451.3 [M + H]⁺ [α]_(D) ²⁰ = +77.2° (c = 1.00, dimethylsulfoxide) 38% yield Chiral HPLC Example 44.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (2552 mg, 5.61 mmol; Example 44) on a chiral column gave 1096 mg (35% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B; 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.81 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 44.2

Example 44 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.01-5.12 (m, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.17 (d, J = 8.62 Hz, 2 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (d, J = 8.87 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 8.15 (br s, 2 H). LC-MS (method 2) Rt = 1.12 min; MS (ESIpos): m/z = 451.4 [M + H]⁺ [α]_(D) ²⁰ = −79.7° (c = 1.00, dimethylsulfoxide) 38% yield Chiral HPLC Example 44.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (2552 mg, 5.61 mmol; Example 44) on a chiral column gave 1139 mg (37% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.61 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 45

Intermediate 48; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.41 (d, J = 6.59 Hz, 3 H), 4.63 (q, J = 6.59 Hz, 1 H), 504- 5.12 (m, 1 H), 6.86 (d, J = 8.87 Hz, 2 H), 7.24 (br d, J = 3.80 Hz, 2 H), 7.42-7.52 (m, 6 H), 7.53- 7.58 (m, 3 H), 8.08 (br s, 2 H). LC-MS (method 2) Rt = 0.85 min; MS (ESIpos): m/z = 454.4 [M + H]⁺ RP-HPLC (method C, basic) 70% yield 45.1, (2R)-(N-[4-amino-5-[4-[2-amino- 45.2, (1R)-methyl-2-oxo- 45.3 and ethoxy]benzoyl]thiazol-2- 45.4 yl]anilino)propanamide, (2R)-(N-[4-amino-5-[4-[2-amino- (1S)-methyl-2-oxo- ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide, (2S)-(N-[4-amino-5-[4-[2-amino- (1R)-methyl-2-oxo- ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide, and (2S)-(N-[4-amino-5-[4-[2-amino- (1S)-methyl-2-oxo- ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide 45.1

Example 45 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.41 (d, J = 6.59 Hz, 3 H), 4.63 (q, J = 6.76 Hz, 1 H), 5.08 (q, J = 7.35 Hz, 1 H), 6.86 (d, J = 8.87 Hz, 2 H), 7.23 (br d, J = 3.30 Hz, 2 H), 7.43-7.52 (m, 6 H), 7.53-7.60 (m, 3 H), 7.75- 8.37 (m, 2 H). LC-MS (method 1) Rt = 0.86 min; MS (ESIpos): m/z = 454.3 [M + H]⁺ 4% yield Chiral HPLC Example 45.1 HPLC separration of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo-ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide (mixture of stereoisomers; 449 mg, 0.99 mmol; Example 45) on a chiral column gave 29 mg (6% yield) of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo- ethoxy]benzoyl]thiazol-2-yl]anilino)propanamide, stereoisomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetontrile; isocratic: 80% A + 20% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 6.48 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm 45.2

Example 45 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.41 (d, J = 6.59 Hz, 3 H), 4.63 (q, J = 6.59 Hz, 1 H), 5.08 (q, J = 7.27 Hz, 1 H), 6.86 (d, J = 8.87 Hz, 2 H), 7.23 (br d, J = 4.82 Hz, 2 H), 7.42-7.52 (m, 6 H), 7.55 (br d, J = 1.27 Hz, 3 H), 8.14 (br s, 2 H). LC-MS (method 1) Rt = 0.85 min; MS (ESIpos): m/z = 454.3 [M + H]⁺ 5% yield Chiral HPLC Example 45.2 HPLC separation of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo-ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide (mixture of stereoisomers; 449 mg, 0.99 mmol; Example 45) on a chiral column gave 36 mg (7% yield) of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo- ethoxy]benzoyl]thiazol-2-yl]anilino)propanamide, stereoisomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 6.48 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm 45.3

Example 45 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.41 (d, J = 6.59 Hz, 3 H), 4.59-4.66 (m, 1 H), 5.08 (q, J = 7.77 Hz, 1 H), 6.86 (d, J = 8.87 Hz, 2 H), 7.23 (br d, J = 4.06 Hz, 2 H), 7.42-7.52 (m, 6 H), 7.53- 7.59 (m, 3 H), 8.05 (br s, 2 H). LC-MS (method 1) Rt = 0.85 min; MS (ESIpos): m/z = 454.3 [M + H]⁺ 5% yield Chiral HPLC Example 45.3 HPLC separation of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo-ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide (mixture of stereoisomers; 449 mg, 0.99 mmol; Example 45) on a chiral column gave 36 mg (8% yield) of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo- ethoxy]benzoyl]thiazol-2-yl]anilino)propanamide, stereoisomer 3. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 10μ, 25 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 6.48 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm 45.4

Example 45 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.41 (d, J = 6.59 Hz, 3 H), 4.63 (q, J = 6.59 Hz, 1 H), 5.08 (q, J = 6.93 Hz, 1 H), 6.86 (d, J = 8.87 Hz, 2 H), 7.23 (br d, J = 3.04 Hz, 2 H), 7.43-7.52 (m, 6 H), 7.53-7.58 (m, 3 H), 8.04 (br s, 2 H). LC-MS (method 2) Rt = 0.86 min; MS (ESIpos): m/z = 454.3 [M + H]⁺ 5% yield Chiral HPLC Example 45.4 HPLC-separation of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo-ethoxy]benzoyl]thiazol-2- yl]anilino)propanamide (mixture of stereoisomers; 449 mg, 0.99 mmol; Example 45) on a chiral column gave 36 mg (8% yield) of 2-(N-[4-amino-5-[4-[2-amino-1-methyl-2-oxo- ethoxy]benzoyl]thiazol-2-yl]anilino)propanamide, stereoisomer 4. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 6.48 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm 46

Intermediate 49; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (s, 3 H), 498- 5.23 (m, 1 H), 7.30 (br t, J = 73.76 Hz, 1 H), 7.19 (br d, J = 8.36 Hz, 2 H), 7.32 (br s, 1 H), 7.48 (br d, J = 8.90 Hz, 1 H), 7.59 (br d, J = 7.35 Hz, 2 H), 7.65 (br s, 1 H), 7.71 (br d, J = 9.12 Hz, 1 H), 7.80-7.91 (m, 1 H), 8.18 (br s, 2 H). LC-MS (Method 1) Rt = 1.21 min; MS (ESIpos): m/z = 485.3 [M + H]⁺ RP-HPLC (method D, basic) 40% yield 46.1 and (R)-2-(N-[4-amino-5-[4- 46.2 (difluoromethoxy)benzoyl]thiazol- 2-yl]-4-chloro-2-fluoro- anilino)propanamide and (S)-2-(N-[4-amino-5-[4- (difluoroethoxy)benzoyl]thiazol- 2-yl]-4-chloro-2-fluoro- anilino)propanamide 46.1

Example 46 ¹H-NMR (400 MHz, DMSO-d6) δ ppm = 1.13-1.22 (m, 3 H), 5.02- 5.22 (m, 1 H), 7.19 (br d, J = 8.36 Hz, 2 H), 7.30 (t, 1 H), 7.32 (br s, 1 H), 7.47-7.50 (m, 1 H), 7.56-7.63 (m, 2 H), 7.63- 7.67 (m, 1 H), 7.68-7.75 (m, 1 H), 7.81-7.94 (m, 1 H), 8.02- 8.36 (m, 2 H). LC-MS (method 1) Rt = 1.21 min; MS (ESIpos): m/z = 485.3 [M + H]⁺ 35% yield Chiral HPLC Example 46.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-2- fluoro-anilino)propanamide (253 mg, 0.52 mmol; Example 46) on a chiral column gave 90 mg (35% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-2-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 70% A + 30% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.42 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 46.2

Example 46 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.12-1.22 (m, 3 H), 4.97-5.23 (m, 1 H), 7.19 (br d, J = 8.36 Hz, 2 H), 7.30 (s, 1 H), 7.32 (br s, 1 H), 74.7-7.51 (m, 1H), 7.59 (br d, J = 7.60 Hz, 2 H), 7.65 (br s, 1 H), 7.69-7.76 (m, 1 H), 7.82-7.92 (m, 1 H), 7.99-8.31 (m, 2 H). LC-MS (method 1) Rt = 1.21 min; MS (ESIpos): m/z = 485.2 [M + H]⁺ 52% yield Chiral HPLC Example 46.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-2- fluoro-anilino)propanamide (253 mg, 0.52 mmol; Example 46) on a chiral column gave 135 mg (52% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-2-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B ethanol + 0.1 vol % diethylamine; isocratic: 70% A + 30% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.13 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 47

Intermediate 50; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (br q, J = 7.27 Hz, 1 H), 7.28 (t, J = 73.51 Hz, 1 H), 7.17 (d, J = 8.62 Hz, 2 H), 7.26 (s, 1 H), 7.54-7.64 (m, 7 H), 8.14 (br s, 2 H). LC-MS (method 2) Rt = 1.19 min; MS (ESIpos): m/z = 467.3 [M + H]⁺ 40% yield 47.1 and (R)-2-(N-[4-amino-5-[4- 47.2 (difluoromethoxy)benzoyl]thiazol- 2-yl]-4-chloro- anilino)propanamide and (S)-2-(N-[4-amino-5-[4- (difluoromethoxy)benozyl]thiazol- 2-yl]-4-chloro- annilino)propanamide 47.1

Example 47 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.18 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.18 (d, J = 8.62 Hz, 2 H), 7.27 (s, 1 H), 7.54-7.64 (m, 7 H), 8.14 (br s, 2 H). LC-MS (method 2) Rt = 1.19 min; MS (ESIpos): m/z = 467.2 [M + H]+ [α]_(D) ²⁰ = +73.5° (c = 1.00, dimethylsulfoxide). RP-HPLC (method D, basic) 11% yield Chiral HPLC Example 47.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro- anilino)propanamide (524 mg, 1.12 mmol; Example 47) on a chiral column gave 147 mg (29% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.99 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluenet B: 2-propanol; isocratic: 60% A temperature: 25° C.; UV: 254 nm 47.2

Example 47 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.18 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.18 (d, J = 8.62 Hz, 2 H), 72.7 (s, 1 H), 7.54-7.64 (m, 7 H), 8.14 (br s, 2 H). LC-MS (method 2) Rt = 1.19 min; MS (ESIpos): m/z = 467.2 [M + H]+ [α]_(D) ²⁰ = −67.1° (c = 1.00, dimethylsulfoxide). RP-HPLC (method D, basic) 13% yield Chiral HPLC Example 47.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro- anilino)propanamide (524 mg, 1.12 mmol; Example 47) on a chiral column gave 178 mg (34% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro- anilino)propanamide, enantiomer 2 Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30, eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.37 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 48

Intermediate 51; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 5.00-5.07 (m, 1 H), 7.29 (t, J = 73.76 Hz, 1 H), 7.19 (d, J = 8.62 Hz, 2 H), 7.30 (s, 1 H), 7.49-7.54 (m, 1 H), 7.58 (d, J = 8.87 Hz, 2 H), 7.63 (s, 1 H), 7.73-7.79 (m, 2 H), 8.19 (br s, 2 H). LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 485.4 [M + H]⁺ RP-HPLC (method D, basic) 25% yield 48.1 and (R)-2-(N-[4-amino-5-[4- 48.2 (difluoromethoxy)benzoyl]thiazol- 2-yl]-4-chloro-3-fluoro- anilino)propanamide and (S)-2-(N-[4-amino-5-[4- (difluoromethoxy)benzoyl]thiazol- 2-yl]-4-chloro-3-fluoro- anilino)propanamide 48.1

Example 48 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.60 Hz, 3 H), 5.03 (q, J = 7.44 Hz, 1 H), 7.29 (t, J = 73.76 Hz, 1 H), 7.19 (d, J = 8.62 Hz, 2 H), 7.30 (s, 1 H), 7.49-7.54 (m, 1 H), 7.58 (d, J = 8.87 Hz, 2 H), 7.63 (s, 1 H), 7.73-7.78 (m, 2 H), 8.17 (br s, 2 H). LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 485.2 [M + H]⁺ 19% yield Chiral HPLC Example 48.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-3- fluoro-anilino)propanamide (144 mg, 0.3 mmol; Example 48) on a chiral column gave 29 mg (20% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B; acetontrile; isocratic: 85% A + 15% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.54 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 85% A + 15% B; flow: 1.4 mL/min; temperature 25° C.; UV: 254 nm 48.2

Example 48 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 5.03 (q, J = 7.10 Hz, 1 H), 7.29 (t, J = 73.76 Hz, 1 H), 7.19 (d, J = 8.87 Hz, 2 H), 7.30 (s, 1 H), 7.51 (dt, J = 8.62, 1.27 Hz, 1 H), 7.58 (d, J = 8.62 Hz, 2 H), 7.63 (s, 1 H), 7.73-7.79 (m, 2 H), 8.18 (br s, 2 H). LC-MS (method 1) Rt = 1.22 min; MS (ESIpos): m/z = 485.3 [M + H]⁺ 24% yield Chiral HPLC Example 48.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-3 fluoro-anilino)propanamide (144 mg, 0.3 mmol; Example 48) on a chiral column gave 36 mg (24% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetontrile; isocratic: 85% A + 15% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.09 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 85% A + 15% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 49

Intermediate 52: rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.03 (q, J = 6.76 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.18 (d, J = 8.62 Hz, 2 H), 7.30 (s, 1 H), 7.48-7.53 (m, 1 H), 7.58 (d, J = 8.62 Hz, 4 H), 7.78 (ddd, J = 11.34, 7.41, 2.53 Hz, 1 H), 8.13 (br s, 2 H). LC-MS (method 1) Rt = 1.16 min; MS (ESIpos): m/z = 469.3 [M + H]⁺ RP-HPLC (method D, basic) 65% yield 49.1 and (R)-2-(N-[4-amino-5-[4- 49.2 (difluoromethoxy)benzoyl]thiazol- 2-yl]-3,4-difluoro- anilino)propanamide and (S)-2-(N-[4-amino-5-[4- (difluoromethoxy)benzoyl]thiazol- 2-yl]-3,4-difluoro- anilino)propanamide 49.1

Example 49 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.03 (br q, J = 7.18 Hz, 1 H), 7.29 (t, J = 73.51 Hz, 3 H), 7.18 (d, J = 8.36 Hz, 1 H), 7.30 (s, 1 H), 7.48-7.53 (m, 1 H), 7.56- 7.64 (m, 4 H), 7.78 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 8.17 (br s, 2 H). LC-MS (method 1) Rt = 1.16 min; MS (ESIpos): m/z = 469.3 [M + H]⁺ 39% yield Chiral HPLC Example 49.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-3,4-difluoro- anilino)propanamide (381 mg, 0.81 mmol; Example 49) on a chiral column gave 155 mg (39% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.31 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic; 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 49.2

Example 49 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 7.28 (t, J = 74.01 Hz, 1 H), 7.18 (d, J = 8.62 Hz, 2 H), 7.30 (s, 1 H), 7.48-7.53 (m, 1 H), 7.55- 7.65 (m, 4 H), 7.78 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 8.19 (br s, 2 H). LC-MS (method 1) Rt = 1.16 min; MS (ESIpos): m/z = 469.3 [M + H]⁺ 34% yield Chiral HPLC Example 49.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-3,4-difluoro- anilino)propanamide (381 mg, 0.81 mmol; Example 49) on a chiral column gave 136 mg (34% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 ×+30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.54 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B; ethnaol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 49.2

Example 49.2 was determined to be (R)-2-(N-[4- amino-5-[4-(difluoromethoxy)benzyl]thiazol-2- yl]-3,4-dihydroanilino)propanamide; by means of X-ray crystal structure analysis. 50

Intermediate 53; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.60 Hz, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 7.30 (s, 1 H), 7.40-7.44 (m, 2 H), 7.47-7.53 (m, 1 H), 7.55-7.66 (m, 2 H), 7.77 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 8.13-8.49 (m, 2 H), 8.60-8.66 (m, 2 H). LC-MS (method 2) Rt = 0.90 min; MS (ESIpos): m/z = 404.3 [M + H]⁺ 97% yield 50.1 and (R)-2-(N-[4-amino-5-(pyridine-4- 50.2 carbonyl)thiazol-2-yl]-3,4- difluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(pyridine-4- carbonyl)thiazol-2-yl]-3,4- difluoro-anilino)propanamide 50.1

Example 50 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.03 (br q, J = 7.27 Hz, 1 H), 7.30 (s, 1 H), 7.40-7.45 (m, 2 H), 7.47-7.53 (m, 1 H), 7.55- 7.65 (m, 2 H), 7.78 (br ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 8.17-8.47 (m, 2 H), 8.60-8.66 (m, 2 H). LC-MS (method 2) Rt = 0.87 min; MS (ESIpos): m/z = 404.2 [M + H]⁺ [α]_(D) ²⁰ = −79.8° (c = 1.00, dimethylsulfoxide) 21% yield Chiral HPLC Example 50.1 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide (974 mg, 2.35 mmol; Example 50) on a chiral column gave 205 mg (21% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 20% A + 80% B; flow: 140 mL/ min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.71 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 20% A + 80% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 50.2

Example 50 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.60 Hz, 3 H), 5.03 (q, J = 6.67 Hz, 1 H), 7.30 (s, 1 H), 7.40-7.44 (m, 2 H), 7.47-7.53 (m, 1H), 7.55- 7.65 (m, 2 H), 7.77 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 8.14-8.46 (m, 2 H), 8.60-8.66 (m, 2 H). LC-MS (method 2) Rt = 0.87 min; MS (ESIpos): m/z = 404.2 [M + H]⁺ [α]_(D) ²⁰ = +84.9° (c = 1.00, dimethylsulfoxide) 21% yield Chiral HPLC Example 50.2 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide (974 mg, 2.35 mmol; Example 50) on a chiral column gave 204 mg (21% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 20% A + 80% B; flow: 140 mL/min; temperature: 25° C.: UV: 254 nm Analytical chiral HPLC: Rt = 3.06 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile: isocratic: 20% A + 80% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 51

Intermediate 54; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.60 Hz, 3 H), 3.76 (s, 3 H), 5.02 (q, J = 7.27 Hz, 1 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.29 (s, 1 H), 7.50 (d, J = 8.62 Hz, 3 H), 7.56-7.65 (m, 2 H), 7.78 (ddd, J = 11.41, 7.48, 2.41 Hz, 1 H), 7.89-8.45 (m, 2 H) LC-MS (method 2) Rt = 1.09 min; MS (ESIpos): m/z = 433.2 [M + H]⁺ 85% yield 51.1 and (R)-2-(N-[4-amino-5-(4- 51.2 methoxybenzoyl)thiazol-2-yl]- 3,4-difluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- methoxybenzoyl)thiazol-2-yl]- 3,4-difluoro-anilino)propanamide 51.1

Example 51 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.03 (q, J = 7.18 Hz, 1 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.29 (s, 1 H), 7.48-7.53 (m, 3 H), 7.55-7.64 (m, 2 H), 7.78 (ddd, J = 11.47, 7.41, 2.41 Hz, 1 H), 7.87-8.36 (m, 2 H). LC-MS (method 1) Rt = 1.10 min; MS (ESIpos): m/z = 433.5 [M + H]⁺ 33% yield Chiral HPLC Example 51.1 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide (88 mg, 0.20 mmol; Example 51) on a chiral column gave 19 mg (21% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic 50% A + 50% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.57 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 51.2

Example 51 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.03 (q, J = 7.10 Hz, 1 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.29 (s, 1 H), 7.48-7.53 (m, 3 H), 7.56-7.65 (m, 2 H), 7.75- 7.82 (m, 1 H), 7.89-8.38 (m, 2 H). LC-MS (method 1) Rt = 1.10 min; MS (ESIpos): m/z = 433.5 [M + H]⁺ 36% yield Chiral HPLC Example 51.2 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide (88 mg, 0.20 mmol; Example 51) on a chiral column gave 22 mg (24% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.84 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: Methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 52

Intermediate 55; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.86 (s, 3 H), 5.03 (q, J = 7.69 Hz, 1 H), 6.84 (dd, J = 8.62, 0.76 Hz, 1 H), 7.30 (s, 1 H), 7.49- 7.54 (m, 1 H), 7.56-7.65 (m, 2 H), 7.79 (ddd, J = 11.41, 7.35, 2.53 Hz, 1 H), 7.84 (dd, J = 8.62, 2.28 Hz, 1 H), 8.16 (br s, 2 H), 8.36 (dd, J = 2.41, 0.63 Hz, 1 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 434.3 [M + H]⁺ RP-HPLC (method C, basic) 48% yield 52.1 and (R)-2-(N-[4-amino-5-(6- 52.2 methoxypyridine-3- carbonyl)thiazol-2-yl]-3,4- difluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(6- methoxypyridine-3- carbonyl)thiazol-2-yl]-3,4- difluoro-anilino)propanamide 52.1

Example 52 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.86 (s, 3 H), 5.03 (q, J = 6.84 Hz, 1 H), 6.84 (dd, J = 8.49, 0.63 Hz, 1 H), 7.30 (s, 1 H), 7.49- 7.54 (m, 1 H), 7.56-7.65 (m, 2 H), 7.79 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 7.84 (dd, J = 8.62, 2.28 Hz, 1 H), 7.97-8.32 (m, 2 H), 8.35-8.37 (m, 1 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 434.3 [M + H]⁺ 31% yield Chiral HPLC Example 52.1 HPLC separation of rac-2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazol-2-yl]-3,4- difluoro-anilino)propanamide (31 mg, 0.07 mmol; Example 52) on a chiral column gave 9 mg (27% yield) of 2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 1. Preparative chiral Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol, % diethylamine; eluent B: acetonitrile + 0.1 vol % diethylamine; isocratic: 50% A + 50% B; flow: 40 mL/ min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.25 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 52.2

Example 52 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.86 (s, 3 H), 5.03 (q, J = 6.93 Hz, 1 H), 6.82-6.86 (m, 1 H), 7.30 (s, 1 H), 7.49-7.54 (m, 1 H), 7.56-7.64 (m, 2 H), 7.79 (ddd, J = 11.28, 7.48, 2.28 Hz, 1 H), 7.84 (dd, J = 8.62, 2.53 Hz, 1 H), 7.96-8.31 (m, 2 H), 8.36 (d, J = 2.03 Hz, 1 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 434.3 [M + H]⁺ 31% yield Chiral HPLC Example 52.2 HPLC separation of rac-2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazol-2-yl]-3,4- difluoro-anilino)propanamide (31 mg, 0.07 mmol; Example 52) on a chiral column gave 7 mg (20% yield) of 2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetontrile + 0.1 vol % diethylamine; isocratic: 50% A + 50% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.74 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 53

Intermediate 56; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.60 Hz, 3 H), 4.99-5.09 (m, 1 H), 7.31 (s, 1H), 7.47-7.54 (m, 1 H), 7.55- 7.67 (m, 2 H), 7.78 (ddd, J = 11.22, 7.54, 2.53 Hz, 1 H), 7.95 (d, J = 7.60 Hz, 1 H), 8.15 (dd, J = 7.98, 1.90 Hz, 1 H), 8.24- 8.49 (m, 2 H), 8.86 (d, J = 1.77 Hz, 1 H). LC-MS (method 1) Rt = 1.16 min; MS (ESIpos): m/z = 472.3 [M + H]⁺ RP-HPLC (method C, basic) 55% yield 53.1 and (R)-2-(N-[4-amino-5-[6- 53.2 (trifluoromethyl)pyridine-3- carbonyl]thiazol-2-yl]-3,4- difluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-[6- (trifluoromethyl)pyridine-3- carbonyl]thiazol-2-yl]-3,4- difluoro-anilino)propanamide 53.1

Example 53 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.60 Hz, 3 H), 5.04 (br q, J = 6.84 Hz, 1 H), 7.31 (s, 1 H), 7.48-7.53 (m, 1 H), 7.55-7.66 (m, 2 H), 7.78 (ddd, J = 11.28, 7.48, 2.28 Hz, 1 H), 7.95 (dd, J = 8.24, 0.63 Hz, 1 H), 8.15 (dd, J = 7.98, 1.90 Hz, 1 H), 8.23-8.51 (m, 2 H), 8.86 (d, J = 1.77 Hz, 1 H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 472.2 [M + H]⁺ 30% yield Chiral HPLC Example 53.1 HPLC separation of rac-2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]- 3,4-difluoro-anilino)propanamide (164 mg, 0.35 mmol; Example 53) on a chiral column gave 51 mg (30% yield) of 2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]-3,4- difluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.05 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 53.2

Example 53 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 5.04 (br q, J = 7.10 Hz, 1 H), 7.31 (s, 1 H), 7.46-7.53 (m, 1 H), 7.55-7.66 (m, 2 H), 7.78 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H), 7.95 (dd, J = 8.24, 0.63 Hz, 1 H), 8.15 (dd, J = 7.98, 1.90 Hz, 1 H), 8.23-8.56 (m, 2 H), 8.86 (d, J = 1.77 Hz, 1 H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 472.2 [M + H]+ 27% yield Chiral HPLC Example 53.2 HPLC separation of rac-2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thiazol-2-yl]- 3,4-difluoro-anilino)propanamide (164 mg, 0.35 mmol; Example 53) on a chiral column gave 47 mg (27% yield) of 2-(N-[4-amino-5-[6-(trifluoromethyl)pyridine-3-carbonyl]thial-2-yl]-3,4- difluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.36 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 54

Intermediate 78; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 2.28 (s, 3H), 5.11 (q, J = 7.10 Hz, 1 H), 7.15-7.25 (m, 3 H), 7.33-7.39 (m, 3 H), 7.61-7.71 (m, 5 H). LC-MS (method 1) Rt = 1.20 min; MS (ESIpos): m/z = 449.1 [M + H]⁺ RP-HPLC (method D, basic) 52% yield 55

Intermediate 79; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 2.27 (s, 3 H), 5.09 (q, J = 7.27 Hz, 1 H), 7.16-7.38 (m, 4 H), 7.51-7.56 (m, 1 H), 7.58-7.69 (m, 4 H), 7.82 (ddd, J = 11.34, 7.54, 2.41 Hz, 1 H). LC-MS (method 1) Rt = 1.23 min; MS (ESIpos): m/z = 466.3 [M + H]⁺ RP-HPLC (method D, basic) 7% yield 55.1 and (R)-2-(N-[5-[4- 55.2 (difluoromethoxy)benzoyl]-4- methyl-thiazol-2-yl]-3,4-difluoro- anilino)propanamide and (S)-2- (N-[5-[4- (difluoromethoxy)benzoyl]-4- methyl-thiazol-2-yl]-3,4-difluoro- anilino)propanamide 55.1

Example 55 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 2.27 (s, 3 H), 5.08-5.10 (m, 1 H), 7.20-7.24 (m, 3 H), 7.35 (t, J = 72 Hz, 1 H), 7.52-7.54 (m, 1 H), 7.60-7.66 (m, 4 H), 7.81- 7.83 (m, 1 H) LC-MS (method 1) Rt = 1.23 min MS (ESIpos): m/z = 468.5 [M + H]⁺ 35% yield Chiral HPLC Example 55.1 HPLC separation of rac-2-(N-[5-[4-(difluoromethoxy)benzoyl]-4-methyl-thiazol-2-yl]-3,4- difluoro-annilino)propanamide (59 mg, 0.13 mmol, Example 55) on a chiral column gave 21 mg (35% yield) of 2-(N-[5-[4-(difluoromethoxy)benzoyl]-4-methyl-thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-2; Column: Chiralpak AS 5μ, 250 × 20; eluent A: hexane + 0.1 vol % diethylamine, eluent B: 2-propanol; isocratic: 80% A + 20% B; flow: 20 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.43 min Instrument: Thermo Fisher UltiMate 3000; Column: Chiralpak AS 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 55.2

Example 55 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 2.27 (s, 3 H), 5.08-5.10 (m, 1 H), 7.20-7.24 (m, 3 H), 7.35 (t, J = 72 Hz, 1 H), 7.52-7.54 (m, 1 H), 7.60-7.66 (m, 4 H), 7.81- 7.83 (m, 1 H) LC-MS (method 1) Rt = 1.23 min MS (ESIpos): m/z = 468.5 [M + H]⁺ 17% yield Chiral HPLC Example 55.2 HPLC separation of rac-2-(N-[5-[4-(difluoromethoxy)benzoyl]-4-methyl-thiazol-2-yl]-3,4- difluoro-anilino)propanamide (59 mg, 0.13 mmol, Example 55) on a chiral column gave 10 mg (17% yield) of 2-(N-[5-[4-(difluoromethoxy)benzoyl]-4-methyl-thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-2; Column: Chiralpak AS 5μ, 250 × 20; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 80% A + 20% B; flow: 20 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 9.88 min Instrument: Thermo Fisher UltiMate 3000; Column: Chiralpak AS 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 56

Intermediate 57; rac-2- bromopropan- amide H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 7.31 (s, 1 H), 7.43 (br d, J = 4.31 Hz, 2 H), 7.49-7.53 (m, 1 H), 7.64 (s, 1 H), 7.71-7.78 (m, 2 H), 8.13-8.48 (m, 2 H), 8.63 (br s, 2 H). LC-MS (method 2) Rt = 0.96 min; MS (ESIpos): m/z = 420.3 [M + H]⁺ RP-HPLC (method C, basic) 33% yield 56.1 and (R)-2-(N-[4-amino-5-(pyridine-4- 56.2 carbonyl)thiazol-2-yl]-4-chloro-3- fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(pyridine-4- carbonyl)thiazol-2-yl]-4-chloro-3- fluoro-anilino)propanamide 56.1

Example 56 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.60 Hz, 3 H), 4.98-5.08 (m, 1 H), 7.31 (s, 1 H), 7.41-7.45 (m, 2 H), 7.48- 7.53 (m, 1 H), 7.64 (s, 1 H), 7.72- 7.77 (m, 2 H), 8.07-8.45 (m, 2 H), 8.62-8.65 (m, 2 H). LC-MS (Method 2) Rt = 0.94 min; MS (ESIpos): m/z = 420.2 [M + H]⁺ 19% yield Chiral HPLC Example 56.1 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide (133 mg, 0.32 mmol; Example 56) on a chiral column gave 26 mg (20% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.10 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 56.2

Example 56 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.60 Hz, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 7.31 (s, 1 H), 7.40-7.46 (m, 2 H), 7.49-7.53 (m, 1 H), 7.64 (s, 1 H), 7.72-7.79 (m, 2 H), 8.12- 8.47 (m, 2 H), 8.61-8.66 (m, 2 H) LC-MS (method 2) Rt = 0.94 min; MS (ESIpos): m/z = 420.2 [M + H]⁺ 20% yield Chiral HPLC Example 56.2 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide (133 mg, 0.32 mmol; Example 56) on a chiral column gave 27 mg (20% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 140 mL/ min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.34 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 57

Intermediate 58; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.27 Hz, 1 H), 7.27(s, 1 H), 7.41 (d, J = 6.08 Hz, 2 H), 7.59 (d, J = 10.90 Hz, 5 H), 8.08-8.48 (m, 2 H), 8.62 (d, J = 6.08 Hz, 2 H). LC-MS (method 2) Rt = 0.91 min; MS (ESIpos): m/z = 402.2 [M + H]⁺ 50% yield 57.1 and (R)-2-(N-[4-amino-5-(pyridine-4- 57.2 carbonyl)thiazol-2-yl]-4-chloro- anilino)propanamide and (S)-2- (N-[4-amino-5-(pyridine-4- carbonyl)thiazol-2-yl]-4-chloro- anilino)propanamide 57.1

Example 57 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.06 (m, 1 H), 7.28 (s, 1 H), 7.41 (m, 2 H), 7.59 (m, 5 H), 8.28 (m, 2 H), 8.62 (m, 2 H) LC-MS (method 1) Rt = 0.85 min MS (ESIpos): m/z = 402.4 [M + H]⁺ [α]_(D) ²⁰ = −67° c = 8.2 mg/mL in DMSO 38% yield Chiral HPLC Example 57.1 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro- anilino)propanamide (590 mg, 1.47 mmol, Example 57) on a chiral column gave 234 mg (38% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IG 5μ 250 × 30 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 4.76 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IG 5μ 100 = 4.6 mm, eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 4 mL/ min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 57.2

Example 57 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.06 (m, 1 H), 7.28 (s, 1 H), 7.41 (m, 2 H), 7.59 (m, 5 H), 8.28 (m, 2 H), 8.62 (m, 2 H) LC-MS (method 1) Rt = 0.85 min MS (ESIpos): m/z = 402.4 [M + H]⁺ [α]_(D) ²⁰ = 76° c = 9.6 mg/mL in DMSO 36% yield Chiral HPLC Example 57.2 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro- anilino)propanamide (590 mg, 1.47 mmol, Example 57) on a chiral column gave 225 mg (36% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IG 5μ 250 × 30 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 100mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 2.59 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IG5 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 4 mL/ min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 58

Intermediate 59; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 6.93 (d, J = 8.87 Hz, 2 H), 7.26 (s, 1 H), 7.49 (d, J = 8.87 Hz, 2 H), 7.55-7.64 (m, 5 H), 7.82-8.39 (m, 2 H). LC-MS (method 2) Rt = 1.13 min; MS (ESIpos): m/z = 431.2 [M + H]⁺ 77% yield 58.1 and (R)-2-(N-[4-amino-5-(4- 58.2 methoxybenzoyl)thiazol-2-yl]-4- chloro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- methoxybenzoyl)thiazol-2-yl]-4- chloro-anillino)propanamide 58.1

Example 58 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.06 (m, 1 H), 6.93 (m, 2 H), 7.25 (m, 1 H), 7.49 (m, 2 H), 7.60 (m, 5 H), 8.04 (m, 2 H) LC-MS (method 1) Rt = 1.14 min MS (ESIpos): m/z = 431.4 [M + H]⁺ [α]_(D) ²⁰ = −80° c = 11.1 mg/mL in DMSO 36% yield Chiral HPLC Example 58.1 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro- anilino)propanamide (2.83 g, 6.57 mmol, Example 58) on a chiral column gave 1.03 g (36% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Labomatic HD3000, Knauer Pump 100, Labcol Vario 4000 Plus, Knauer DAD 2600; Column: Cellulose SB 5μ 250 × 50 mm Nr. 034; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic 60% A + 40% B; flow 100.0 mL/ min, UV @ 254 nm Analytical chiral HPLC: Rt = 4.15 min Instrument: Agilent HPLC 1260; Column: Cellulose SB 3μ 100 × 4.6 mm; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% Diethylamine (99%); isocratic: 60% A + 40% B; flow 1.0 mL/min; temperature: 25° C.; DAD 254 nm 58.2

Example 58 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.06 (m, 1 H), 6.93 (m, 2 H), 7.25 (m, 1 H), 7.49 (m, 2 H), 7.60 (m, 5 H), 8.04 (m, 2 H) LC-MS (method 1) Rt = 1.14 min MS (ESIpos): m/z = 431.4 [M + H]⁺ [α]_(D) ²⁰ = 79° c = 11.1 mg/mL in DMSO 41% yield Chiral HPLC Example 58.2 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro- anilino)propanamide (2.83 g, 6.57 mmol, Example 58) on a chiral column gave 1.18 g (41% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Labomatic HD3000, Knauer Pump 100, Labcol Vario 4000 Plus, Knauer DAD 2600; Column: Cellulose SB 5μ 250 × 50 mm Nr. 034; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic: 60% A + 40% B; flow 100.0 mL/min, UV @ 254 nm Analytical chiral HPLC: Rt = 3.40 min Instrument: Agilent HPLC 1260; Column: Cellulose SB 3μ 100 × 4.6 mm; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic: 60% A + 40% B; flow 1.0 mL/min; temperature: 25° C.; DAD 254 nm 59

Intermediate 60; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.77 (s, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.30 (s, 1 H), 7.49-7.53 (m, 3 H), 7.63 (s, 1 H), 7.73-7.79 (m, 2 H), 7.88-8.31 (m, 2 H). LC-MS (method 2) Rt = 1.17 min; MS (ESIpos): m/z = 449.3 [M + H]⁺ 83% yield 59.1 and (R)-2-(N-[4-amino-5-(4- 59.2 methoxybenzoyl)thiazol-2-yl]-4- chloro-3-fluoro- anilino)propanamide and (S)-2- (N-[4-amino-5-(4- methoxybenzoyl)thiazol-2-yl]-4- chloro-3-fluoro- anilino)propanamide 59.1

Example 59 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 6.94 (m, 2 H), 7.30 (s, 1 H), 7.52 (m, 3 H), 7.63 (s, 1 H), 7.75 (m, 2 H), 8.10 (m, 2 H) LC-MS (method 1) Rt = 1.16 min MS (ESIpos): m/z = 449.4 [M + H]⁺ [α]_(D) ²⁰ = −82° c = 8.2 mg/mL in DMSO 29% yield Chiral HPLC Example 59.1 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-3-fluroo- anilino)propanamide (442 mg, 0.98 mmol, Example 59) on a chiral column gave 132 mg (29% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantioemr 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetontrile; isocratic 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.49 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 59.1

Example 59.1 was determined to be (R)-2-(N-[4- amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4- chloro-3-fluoro-anilino)propanamide, by means of X-ray crystal structure analysis. 59.2

Example 59 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.76 (s, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 6.94 (m, 2 H), 7.30 (s, 1 H), 7.52 (m, 3 H), 7.63 (s, 1 H), 7.75 (m, 2 H), 8.10 (m, 2 H) LC-MS (method 1) Rt = 1.16 min MS (ESIpos): m/z = 449.4 [M + H]⁺ [α]_(D) ²⁰ = 87° c = 7.6 mg/mL in DMSO 15% yield Chiral HPLC Example 59.2 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide (442 mg, 0.98 mmol, Example 59) on a chiral column gave 68 mg (15% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine, eluent B: acetontrile; isocratic: 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.03 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 60

Intermediate 83; rac-2- bromopropan- amide ¹H-NMR (600 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.25 Hz, 3 H), 5.09 (q, J = 7.25 Hz, 1 H), 7.24 (s, 1 H), 7.35-7.40 (m, 3 H), 7.42-7.50 (m, 5 H), 7.54- 7.57 (m, 3 H), 7.74-8.52 (m, 2 H). LC-MS (method 2) Rt = 1.02 min; MS (ESIpos): m/z ≤ 367.1 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 46% yield 61

Intermediate 84; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.00-5.10 (m, 1 H), 7.25 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.35-7.42 (m, 3 H), 7.45-7.52 (m, 2 H), 7.58 (s, 1 H), 7.63 (dd, J = 9.00, 5.20 Hz, 2 H), 7.80- 8.46 (m, 2 H). LC-MS (method 2) Rt = 1.07 min; MS (ESIpos): m/z = 385.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 87% yield 61.1 and (R)-2-(N-(4-amino-5-benzoyl- 61.2 thiazol-2-yl)-4-fluoro- anilino)propanamide and (S)-2-(N-(4-amino-5-benzoyl- thiazol-2-yl)-4-fluoro- anilino)propanamide 61.1

Example 61 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.60 Hz, 3 H), 5.02-5.09 (m, 1 H), 7.25 (s, 1H), 7.32 (t, J = 8.87 Hz, 2 H), 7.36-7.42 (m, 3 H), 7.46-7.50 (m, 2 H), 7.58 (s, 1 H), 7.63 (dd, J = 8.74, 5.20 Hz, 2 H), 7.80- 8.50 (m, 2 H). LC-MS (method 1) Rt = 1.07 min; MS (ESIpos): m/z = 385.5 [M + H]⁺ 24% yield Chiral HPLC Example 61.1 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-4-fluoro-anilino)propanamide (414 mg, 1.08 mmol; Example 61) on a chiral column gave 139 mg (33% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IA 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 25% B; flow: 100 mL/ min; temperature: 40° C.; BPR: 150 bar; UV: 280 nm Analytical chiral HPLC: Rt = 2.10 min Instrument: Agilent: 1260, Aurora SFC-Module; column Chiralpak IA 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4% diethylamine (99%); isocratic: 25% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 280 nm 61.2

Example 61 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.60 Hz, 3 H), 5.02-5.09 (m, 1 H), 7.25 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.36-7.42 (m, 3 H), 7.46-7.50 (m, 2 H), 7.58 (s, 1 H), 7.63 (dd, J = 8.74, 5.20 Hz, 2 H), 7.80- 8.50 (m, 2 H). LC-MS (Method 1) Rt = 1.07 min; MS (ESIpos): m/z = 385.5 [M + H]⁺ 26% yield Chiral HPLC Example 61.2 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-4-fluoro-anilino)propanamide (414 mg, 1.08 mmol; Example 61) on a chiral column gave 103 mg (24% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IA 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 25% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 280 nm Analytical chiral HPLC: Rt = 3.08 min Instrument: Agilent: 1260, Aurora SFC-Modul; column: Chiralpak IA 5μ 100 × 4.6 mm; eluent A: CO2; eluent B: 2-Propanol + 0.4% diethylamine (99%); isocratic: 25% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 280 nm 61.2

Example 61.2 was determined to be (R)-2-(N- (4-amino-5-benzoyl-thiazol-2-yl)-4-fluoro- anilino)propanamide, by means of X-ray crystal structure analysis. 62

Intermediate 85; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 3.75 (s, 3 H), 5.06 (q, J = 6.76 Hz, 1 H), 6.93 (d, J = 8.87 Hz, 2 H), 7.22-7.27 (m, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.48 (d, J = 8.87 Hz, 2 H), 7.57 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.79- 8.38 (m, 2 H). LC-MS (method 2) Rt = 1.06 min; MS (ESIpos): m/z = 415.5 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 49% yield 62.1 and (R)-2-(N-[4-amino-5-(4- 62.2 methoxybenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- methoxybenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide 62.1

Example 62 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 3.75 (s, 3 H), 5.06 (q, J = 6.76 Hz, 1 H), 6.93 (d, J = 8.87 Hz, 2 H), 7.22-7.27 (m, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.48 (d, J = 8.87 Hz, 2 H), 7.57 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.79- 8.38 (m, 2 H). [α]_(D) ²⁰ = −142.9° (c = 1.00, chloroform) 45% yield Chiral HPLC Example 62.1 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (350 mg, 0.80 mmol; Example 62) on a chiral column gave 135 mg (45% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Pre SFC100; Column: Chiralpak IA 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 30% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 1.72 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IA 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 30% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 62.2

Example 62 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 3.75 (s, 3 H), 5.06 (q, J = 6.76 Hz, 1 H), 6.93 (d, J = 8.87 Hz, 2 H), 7.2-7.27 (m, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.48 (d, J = 8.87 Hz, 2 H), 7.57 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.79- 8.38 (m, 2 H). [α]_(D) ²⁰ = +114.5° (c = 1.00, chloroform) 49% yield Chiral HPLC Example 62.2 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (350 mg, 0.80 mmol; Example 62) on a chiral column gave 15 mg (49% yield) of 2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IA 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 30% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 2.61 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column; Chiralpak IA 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 30% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar, UV: 254 nm 62.2

Example 62.2 was determined to be (R)-2-(N-[4- amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide, by means of X-ray crystal structure analysis. 63

Intermediate 86; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 2.29 (s, 3 H), 5.05 (q, J = 6.67 Hz, 1 H), 7.18 (d, J = 8.11 Hz, 2 H), 7.24 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.38 (d, J = 8.11 Hz, 2 H), 7.57 (s, 1 H), 7.63 (dd, J = 8.62, 5.07 Hz, 2 H), 8.10 (br s, 2 H). LC-MS (method 2) Rt = 1.15 min; MS (ESIpos): m/z = 399.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 18% yield 63.1 and (R)-2-(N-[4-amino-5-(4- 63.2 methylbenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(4- methylbenzoyl)thiazol-2-yl]-4- fluoro-anilino)propanamide 63.1

Example 63 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 2.29 (s, 3 H), 5.05 (q, J = 6.67 Hz, 1 H), 7.18 (d, J = 8.11 Hz, 2 H), 7.24 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.38 (d, J = 8.11 Hz, 2 H), 7.57 (s, 1 H), 7.63 (dd, J = 8.62, 5.07 Hz, 2 H), 8.10 (br s, 2 H). [α]_(D) ²⁰ = −159.9° (c = 1.00, chloroform) 13% yield Chiral HPLC Example 63.1 HPLC separation of rac-2-(N-[4-amino-5-(4-methylbenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (1.5 g, 3.58 mmol; Example 63) on a chiral column gave 660 mg (45% yield) of 2-(N-[4-amino-5-(4-methylbenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt =0 2.46 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow;: 1.4 mL/ min; temperature: 25° C.; UV: 254 nm 63.2

Example 63 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3 H), 2.29 (s, 3H), 5.05 (q, J = 6.67 Hz, 1 H), 7.18 (d, J = 8.11 Hz, 2 H), 7.24 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.38 (d, J = 8.11 Hz, 2 H), 7.57 (s, 1 H), 7.63 (dd, J = 8.62, 5.07 Hz, 2 H), 8.10 (br s, 2 H). [α]_(D) ²⁰ = +147.0° (c = 1.00, chloroform) 13% yield Chiral HPLC Example 63.2 HPLC separation of rac-2-(N-[4-amino-5-(4-methylbenzoyl)thiazol-2-yl]-4-fluoro- anilino)propanamide (1.5 g, 3.58 mmol; Example 63) on a chiral column gave 680 mg (44% yield) of 2-(N-[4-amino-5-(4-methylbenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.69 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 64

Intermediate 87; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMOS-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (br q, J = 6.84 Hz, 1 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (dd, J = 8.74, 5.45 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.86- 8.56 (m, 2 H). LC-MS (method 2) Rt = 1.09 min; MS (ESIpos): m/z = 403.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 7% yield 65

Intermediate 88; rac-2- bromopropan- amide ¹H-NMR (400 MHz, CHLOROFORM-d): δ ppm = 1.27 (d, J = 7.10 Hz, 3 H), 5.23 (q, J = 7.18 Hz, 1 H), 5.39-5.48 (m, 1 H), 6.58-6.70 (m, 1 H), 6.94-7.02 (m, 2 H), 7.33-7.43 (m, 4 H), 7.58-7.64 (m, 2 H) (NH2 missing). LC-MS (method 2) Rt = 1.08 min; MS (ESIpos): m/z = 403.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 29% 66

Intermediate 89; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 6.84 Hz, 3 H), 3.78 (s, 3 H), 5.06 (q, J = 6.59 Hz, 1 H), 7.00 (d, J = 9.12 Hz, 2 H), 7.21 (s, 1 H), 7.35-7.42 (m, 3 H), 7.43-7.49 (m, 4 H), 7.52 (s, 1 H), 7.65-8.49 (m, 2 H). LC-MS (method 2) Rt = 1.03 min; MS (ESIpos): m/z = 397.4 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 19% yield 67

Intermediate 83; rac-2- bromobutana- mide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 0.84 (t, J = 7.35 Hz, 3 H), 1.47-1.60 (m, 1 H), 1.69- 1.81 (m, 1 H), 4.87-4.95 (m, 1 H), 7.30 (s, 1 H), 7.34-7.51 (m, 8 H), 7.54-7.58 (m, 3 H), 7.78- 8.48 (m, 2 H). LC-MS (method 2) Rt = 1.09 min; MS (ESIpos): m/z = 381.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 43% yield 68

Intermediate 84; rac-2- bromobutana- mide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 0.84 (t, J = 7.35 Hz, 3 H), 1.46-1.58 (m, 1 H), 1.68- 1.79 (m, 1 H), 4.85-4.92 (m, 1 H), 7.32 (s, 3 H), 7.37-7.42 (m, 3 H), 7.47-7.50 (m, 2 H), 7.59 (s, 1 H), 7.64 (dd, J = 9.12, 5.07 Hz, 2 H), 7.83-8.36 (m, 2 H). LC-MS (method 2) Rt = 1.12 min; MS (ESIpos): m/z = 399.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 29% yield 69

Intermediate 90; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.41 (d, J = 6.59 Hz, 3 H), 4.64 (q, J = 6.59 Hz, 1 H), 5.05 (q, J = 6.76 Hz, 1 H), 6.87 (d, J = 8.87 Hz, 2 H), 7.25 (br s, 2 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.46 (d, J = 8.62 Hz, 2 H), 7.50 (s, 1 H), 7.57 (s, 1 H), 7.64 (dd, J = 9.00, 5.20 Hz, 2 H), 7.78-8.35 (m, 2 H). LC-MS (method 2) Rt = 0.87 min; MS (ESIpos): m/z = 572.2 [M + H]⁺ preparative flash chromatography (method Z, 0- 8%) 28% yield 70

Intermediate 85; rac-2- bromobutana- mide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 0.84 (t, J = 7.22 Hz, 3 H), 1.46-1.58 (m, 1 H), 1.67- 1.78 (m, 1 H), 3.76 (s, 3 H), 4.85- 4.93 (m, 1 H), 6.93 (d, J = 8.87 Hz, 2 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.32 (br s, 1 H), 7.49 (d, J = 8.87 Hz, 2 H), 7.59 (s, 1 H), 7.65 (dd, J = 9.00, 4.94 Hz, 2 H), 8.11 (br s, 2 H). LC-MS (method 2) Rt = 1.12 min; MS (ESIpos): m/z = 429.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 28% yield 71

Intermediate 84; 2- bromoaceta- mide ¹H-NMR (500 MHz, DMSO-d6): δ ppm = 4.44 (s, 2 H), 7.23 (s, 1 H), 7.32 (t, J = 8.67 Hz, 2 H), 7.38- 7.43 (m, 3 H), 7.50-7.53 (m, 2 H), 7.56 (s, 1 H), 7.61 (dd, J = 8.83, 5.04 Hz, 2 H), 7.94- 8.44 (m, 2 H). LC-MS (method 2) Rt = 1.00 min; MS (ESIpos): m/z = 371.4 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 8% yield 72

Intermediate 86; 2- bromoaceta- mide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 2.30 (s, 3 H), 4.44 (s, 2 H), 7.18-7.24 (m, 3 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.42 (d, J = 8.11 Hz, 2 H), 7.55 (s, 1 H), 7.61 (dd, J = 9.13, 5.07 Hz, 2 H), 7.79- 8.41 (m, 2 H). LC-MS (method 2) Rt = 1.07 min; MS (ESIpos): m/z = 385.5 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 6% yield 73

Intermediate 91; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 2.33 (s, 3 H), 5.07 (q, J = 7.01 Hz, 1 H), 7.21 (s, 1 H), 7.27 (d, J = 8.11 Hz, 2 H), 7.35-7.43 (m, 5 H), 7.44-7.48 (m, 2 H), 7.52 (s, 1 H), 8.10 (br s, 2 H). LC-MS (method 2) Rt = 1.10 min; MS (ESIpos): m/z = 381.5 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 35% yield 73.1 and (R)-2-(N-(4-amino-5-benzoyl- 73.2 thiazol-2-yl)-4-methyl- anilino)propanamide and (S)-2-(N-(4-amino-5-benzoyl- thiazol-2-yl)-4-methyl- anilino)propanamide 73.1

Example 73 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 2.33 (s, 3 H), 5.07 (q, J = 7.01 Hz, 1 H), 7.21 (s, 1 H), 7.27 (d, J = 8.11 Hz, 2 H), 7.35-7.43 (m, 5 H), 7.44-7.48 (m, 2 H), 7.52 (s, 1 H), 8.10 (br s, 2 H). [α]_(D) ²⁰ = −173.6° (c = 1.00, chloroform) 35% yield Chiral HPLC Example 73.1 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-4-methyl-anilino)propanamide (440 mg, 1.10 mmol; Example 73) on a chiral column gave 160 mg (36% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-4-methyl-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IA 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic; 25% B; flow: 100 mL/ min; temperature: 40° C.; BPR: 150 bar; UV: 280 nm Analytical chiral HPLC: Rt = 2.88 min Instrument: Agilent: 1260, Aurora SFC-module; column: Chiralpak IA 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine, isocratic: 25% B; flow: 4 mL/ min; temperature: 37.5° C.; BPR: 100 bar; UV: 280 nm 73.2

Example 73 H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3 H), 2.33 (s, 3 H), 5.07 (q, J = 7.01 Hz, 1 H), 7.21 (s, 1 H), 7.27 (d, J = 8.11 Hz, 2 H), 7.35-7.43 (m, 5 H), 7.44-7.48 (m, 2 H), 7.52 (s, 1 H), 8.10 (br s, 2 H). [α]_(D) ²⁰ = +161.4° (c = 1.00, chloroform) 8% yield Chiral HPLC Example 73.2 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-4-methyl-anilino)propanamide (440 mg, 1.10 mmol; Example 73) on a chiral column gave 100 mg (23% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-4-methyl-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IA 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 25% B; flow: 100 mL/ min; temperature: 40° C.; BPR: 150 bar; UV: 280 nm Analytical chiral HPLC: Rt = 4.57 min Instrument: Agilent: 1260, Aurora SFC-module; column: Chiralpak IA 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine, isocratic: 25% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 280 nm 74

Intermediate 92; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.28 (s, 1 H), 7.32 (td, J = 8.30, 2.15 Hz, 1 H), 7.37-7.46 (m, 4 H), 7.47-7.56 (m, 4 H), 7.60 (s, 1 H), 7.85-8.35 (m, 2 H). LC-MS (method 2) Rt = 1.06 min; MS (ESIpos): m/z = 385.5 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 15% yield 74.1 and (R)-2-(N-(4-amino-5-benzoyl- 74.2 thiazol-2-yl)-3-fluoro- anilino)propanamide and (S)-2-(N-(4-amino-5-benzoyl- thiazol-2-yl)-3-fluoro- anilino)propanamide 74.1

Example 74 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.28 (s, 1 H), 7.32 (td, J = 8.30, 2.15 Hz, 1 H), 7.37-7.46 (m, 4 H), 7.47-7.56 (m, 4 H), 7.60 (s, 1 H), 7.85-8.35 (m, 2 H). [α]_(D) ²⁰ = −143.6° (c = 1.00, chloroform) 4% yield Chiral HPLC Example 74.1 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-3-fluoro-anilino)propanamide (260 mg, 0.64 mmol; Example 74) on a chiral column gave 65 mg (25% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-3-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: hexane; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 150 mL//min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.46 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: hexane; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 74.2

Example 74 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.28 (s, 1 H), 7.32 (td, J = 8.30, 2.15 Hz, 1 H), 7.37-7.46 (m, 4 H), 7.47-7.56 (m, 4 H), 7.60 (s, 1 H), 7.85-8.35 (m, 2 H). [α]_(D) ²⁰ = +151.0° (c = 1.00, chloroform) 4% yield Chiral HPLC Example 74.2 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-3-fluoro-anilino)propanamide (260 mg, 0.64 mmol; Example 74) on a chiral column gave 65 mg (25% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-3-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: hexane; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 6.76 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: hexane; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C; UV: 254 nm 75

Intermediate 93; rac-2- bromopropan- amide H-NMR (500 MHz, DMSO-d6): δ ppm = 1.22 (d, J = 7.31 Hz, 3 H), 5.04 (q, J = 7.10 Hz, 1 H), 6.87-7.30 (m, 2 H), 7.32 (td, J = 7.63, 1.27 Hz, 1 H), 7.34- 7.43 (m, 4 H), 7.49-7.55 (m, 3 H), 7.77 (td, J = 7.95, 1.59 Hz, 1 H), 7.92 (br s, 2 H). LC-MS (method 2) Rt = 1.04 min; MS (ESIpos): m/z = 385.4 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 52%) yield 75.1 and (R)-2-(N-(4-amino-5-benzoyl- 75.2 thiazol-2-yl)-2-fluoro- anilino)propanamide and (S)-2-(N-(4-amino-5-benzoyl- thiazol-2-yl)-2-fluoro- anilino)propanamide 75.1

Example 75 ¹H-NMR (500 MHz, DMSO-d6): δ ppm = 1.22 (d, J = 7.31 Hz, 3 H), 5.04 (q, J = 7.10 Hz, 1 H), 6.87-7.30 (m, 2 H), 7.32 (td, J = 7.63, 1.27 Hz, 1 H), 7.34- 7.43 (m, 4 H), 7.49-7.55 (m, 3 H), 7.77 (td, J = 7.95, 1.59 Hz, 1 H), 7.92 (br s, 2 H). [α]_(D) ²⁰ = −116.9° (c = 1.00, chloroform) 13% yield Chiral HPLC Example 75.1 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-2-fluoro-anilino)propanamide (560 mg, 1.38 mmol; Example 75) on a chiral column gave 135 mg (24% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-2-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.50 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 75.2

Example 75 ¹H-NMR (500 MHz, DMSO-d6): δ ppm = 1.22 (d, J = 7.31 Hz, 3 H), 5.04 (q, J = 7.10 Hz, 1 H), 6.87-7.30 (m, 2 H), 7.32 (td, J = 7.63, 1.27 Hz, 1 H), 7.34- 7.43 (m, 4 H), 7.49-7.55 (m, 3 H), 7.77 (td, J = 7.95, 1.59 Hz, 1 H), 7.92 (br s, 2 H). [α]_(D) ²⁰ = +109.3° (c = 1.00, chloroform) 15% yield Chiral HPLC Example 75.2 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-2-fluoro-anilino)propanamide (560 mg, 1.38 mmol; Example 75) on a chiral column gave 165 mg (31% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-2-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A; hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.39 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 76

Intermediate 72; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 2.31 (s, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.22 (s, 1 H), 7.23- 7.27 (m, 1 H), 7.33-7.41 (m, 6 H), 7.45-7.50 (m, 2 H), 7.53 (s, 1 H), 7.70-8.47 (m, 2 H). LC-MS (method 2) Rt = 1.10 min; MS (ESIpos): m/z = 381.5 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 71% yield 76.1 and (R)-2-(N-(4-amino-5-benzoyl- 76.2 thiazol-2-yl)-3-methyl- anilino)propanamide and (S)-2-(N-(4-amino-5-benzoyl- thiazol-2-yl)-3-methyl- anilino)propanamide 76.1

Example 76 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 2.31 (s, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.22 (s, 1 H), 7.23- 7.27 (m, 1 H), 7.33-7.41 (m, 6 H), 7.45-7.50 (m, 2 H), 7.53 (s, 1 H), 7.70-8.47 (m, 2 H). [α]_(D) ²⁰ = +177.3° (c = 1.00, chloroform) 31% yield Chiral HPLC Example 76.1 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-3-methyl-anilino)propanamide (1170 mg, 3.00 mmol; Example 76) on a chiral column gave 525 mg (44% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-3-methyl-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetontrile; isocratic: 95% A + 5% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 6.56 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 76.2

Example 76 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 2.31 (s, 3 H), 5.05 (q, J = 7.18 Hz, 1 H), 7.22 (s, 1 H), 7.23- 7.27 (m, 1 H), 7.33-7.41 (m, 6 H), 7.45-7.50 (m, 2 H), 7.53 (s, 1 H), 7.70-8.47 (m, 2 H). [α]_(D) ²⁰ = +177.3° (c = 1.00, chloroform) 34% yield Chiral HPLC Example 76.2 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-3-methyl-anilino)propanamide (1170 mg, 3.00 mmol; Example l76) on a chiral column gave 525 mg (45% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-3-methyl-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic; 95% A + 5% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 8.91 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether; eluent B: acetontrile; isocratic: 95% A + 5% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 77

Intermediate 73; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 0.97-1.08 (m, 3 H), 2.17 (s, 3 H), 4.97-5.12 (m, 1 H), 7.24 (br s, 1 H), 7.33-7.40 (m, 6 H), 7.46 (br d, J = 5.32 Hz, 2 H), 7.57 (br s, 1 H), 7.71-7.77 (m, 1 H), 7.85-8.48 (m, 2 H). LC-MS (method 2) Rt = 1.08 min; MS (ESIpos): m/z = 381.4 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 56% yield 77.1 and (R)-2-(N-(4-amino-5-benzoyl- 77.2 thiazol-2-yl)-2-methyl- anilino)propanamide and (S)-2-(N-(4-amino-5-benzoyl- thiazol-2-yl)-2-methyl- anilino)propanamide 77.1

Example 77 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 0.97-1.08 (m, 3 H), 2.17 (s, 3 H), 4.97-5.12 (m, 1 H), 7.24 (br s, 1 H), 7.33-7.40 (m, 6 H), 7.46 (br d, J = 5.32 Hz, 2 H), 7.57 (br s, 1 H), 7.71-7.77 (m, 1 H), 7.85-8.48 (m, 2 H). [α]_(D) ²⁰ = +166.1° (c = 1.00, chloroform) 23% yield Chiral HPLC Example 77.1 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-2-methyl-anilino)propanamide (1010 mg, 2.52 mmol; Example 77) on a chiral column gave 420 mg (42% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-2-methyl-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: Chiralpak IG 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 85% A + 15% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 7.09 min Instrument: Waters Alliance 2695; Column: Chiralpak IG 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 85% A + 15% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 77.2

Example 77 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 0.97-1.08 (m, 3 H), 2.17 (s, 3 H), 4.97-5.12 (m, 1 H), 7.24 (br s, 1 H), 7.33-7.40 (m, 6 H), 7.46 (br d, J = 5.32 Hz, 2 H), 7.57 (br s, 1 H), 7.71-7.77 (m, 1 H), 7.85-8.48 (m, 2 H). [α]_(D) ²⁰ = −152.0° (c = 1.00, chloroform) 23% yield Chiral HPLC Example 77.2 HPLC separation of rac-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-2-methyl-anilino)propanamide (1010 mg, 2.52 mmol; Example 77) on a chiral column gave 420 mg (42% yield) of 2-(N-(4- amino-5-benzoyl-thiazol-2-yl)-2-methyl-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: Chiralpak IG 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 85% A + 15% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 10.30 min Instrument: Waters Alliance 2695; Column: Chiralpak IG 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 85% A + 15% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 78

Intermediate 94; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.83 (s, 3H), 4.99-5.18 (m, 1 H), 7.18 (s, 1 H), 7.37-7.44 (m, 3 H), 7.48 (s, 1 H), 7.49- 7.53 (m, 3 H), 7.80-8.48 (m, 2 H), 7.90 (s, 1 H). LC-MS (method 2) Rt = 0.80 min; MS (ESIpos): m/z = 371.3 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 17% yield 78.1 and (R)-2-[(4-amino-5-benzoyl- 78.2 thiazol-2-yl)-(1-methylpyrazol-4- yl)amino]propanamide and (S)-2-[(4-amino-5-benzoyl- thiazol-2-yl)-(1-methylpryazol-4- yl)amino]propanamide 78.1

Example 78 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.83 (s, 3H), 4.99-5.18 (m, 1 H), 7.18 (s, 1 H), 7.37-7.44 (m, 3 H), 7.48 (s, 1 H), 7.49- 7.53 (m, 3 H), 7.80-8.48 (m, 2 H), 7.90 (s, 1 H). [α]_(D) ²⁰ = −82.8° (c = 1.00, chloroform) 6% yield Chiral HPLC Example 78.1 HPLC separation of rac-2-[(4-amino-5-benzoyl-thiazol-2-yl)-(1-methylpyrazol-4- yl)amino]propanamide (240 mg, 0.64 mmol; Example 78) on a chiral column gave 89 mg (36% yield) of 2-[(4-amino-5-benzoyl-thiazol-2-yl)-(1-methylpyrazol-4-yl)amino]propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IC 5μ 250 × 30 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic: 40% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 1.77 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IC 5μ 100 × 4.6 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic: 40% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 78.2

Example 78 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.83 (s, 3H), 4.99-5.18 (m, 1 H), 7.18 (s, 1 H), 7.37-7.44 (m, 3 H), 7.48 (s, 1 H), 74.9- 7.53 (m, 3 H), 7.80-8.48 (m, 2 H), 7.90 (s, 1 H). [α]_(D) ²⁰ = +72.6° (c = 1.00, chloroform) 6% yield Chiral HPLC Exmaple 78.2 HPLC separation of rac-2-[(4-amino-5-benzoyl-thiazol-2-yl)-(1-methylpyrazol-4- yl)amino]propanamide (240 mg, 0.64 mmol; Example 78) on a chiral column gave 80 mg (33% yield) of 2-[(4-amino-5-benzoyl-thiazol-2-yl)-(1-methylpyrazol-4-yl)amino]propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IC 5μ 250 × 30 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic: 40% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 2.66 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IC 5 μ 100 × 4.6 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic: 40% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 79

Intermediate 95; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.07 (q, J = 7.10 Hz, 1 H), 5.76 (s, 1 H), 7.30 (s, 1 H), 7.35- 7.43 (m, 3 H), 7.47-7.51 (m, 2 H), 7.54 (dd, J = 8.11, 4.82 Hz, 1 H), 7.64 (s, 1 H), 7.90-8.44 (m, 2 H), 8.04-8.08 (m, 1 H), 8.63 (dd, J = 4.56, 1.52 Hz, 1 H), 8.74 (d, J = 2.03 Hz, 1 H). LC-MS (method 2) Rt = 0.84 min; MS (ESIpos): m/z = 368.5 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 10% yield 79.1 and (R)-2-[(4-amino-5-benzoyl- 79.2 thiazol-2-yl)-(3- pyridyl)amino]propanamide and (S)-2-[(4-amino-5-benzoyl- thiazol-2-yl)-(3- pyridyl)amino]propanamide 79.1

Example 79 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.07 (q, J = 7.10 Hz, 1 H), 5.76 (s, 1 H), 7.30 (s, 1 H), 7.35- 7.43 (m, 3 H), 7.47-7.51 (m, 2 H), 7.54 (dd, J = 8.11, 4.82 Hz, 1 H), 7.64 (s, 1 H), 7.90-8.44 (m, 2 H), 8.04-8.08 (m, 1 H), 8.63 (dd, J = 4.56, 1.52 Hz, 1 H), 8.74 (d, J = 2.03 Hz, 1 H). [α]_(D) ²⁰ = −119.3° (c = 1.00, chloroform) 3% yield Chiral HPLC Example 79.1 HPLC separation of rac-2-[(4-amino-5-benzoyl-thiazol-2-yl)-(3-pyridyl)amino]propanamide (200 mg, 0.52 mmol; Example 79) on a chiral column gave 50 mg (25% yield) of 2-[(4-amino-5- benzoyl-thiazol-2-yl)-(3-pyridyl)amino]propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.15 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm 79.2

Example 79 ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 5.07 (q, J = 7.10 Hz, 1 H), 5.76 (s, 1 H), 7.30 (s, 1 H), 7.35- 7.43 (m, 3 H), 7.47-7.51 (m, 2 H), 7.54 (dd, J = 8.11, 4.82 Hz, 1 H), 7.64 (s, 1 H), 7.90-8.44 (m, 2 H), 8.04-8.08 (m, 1 H), 8.63 (dd, J = 4.56, 1.52 Hz, 1 H), 8.74 (d, J = 2.03 Hz, 1 H). [α]_(D) ²⁰ = +138.1° (c = 1.00, chloroform) 3% yield Chiral HPLC Example 79.2 HPLC separation of rac-2-[(4-amino-5-benzoyl-thiazol-2-yl)-(3-pyridyl)amino]propanamide (200 mg, 0.52 mmol; Example 79) on a chiral column gave 50 mg (25% yield) of 2-[(4-amino-5- benzoyl-thiazol-2-yl)-(3-pyridyl)amino]propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 150 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.15 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm 80

Intermediate 96; rac-2- bromopropan- amide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 6.25 Hz, 1 H), 7.25 (s, 1 H), 7.33 (t, J = 8.74 Hz, 2 H), 7.40-7.46 (m, 2 H), 7.57- 7.67 (m, 5 H), 7.82-8.54 (m, 2 H). LC-MS (method 2) Rt = 1.20 min; MS (ESIpos): m/z = 463.4 [M + H]⁺ preparative flash chromatography (method Z, 0- 3%) 19% yield

Example 81 Rac-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate

Ethyl 2-[4-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]phenoxy]acetate (18.54 g, 44.6 mmol; Intermediate 80) was dissolved in N,N-dimethylformamide (493 mL), followed by the addition of potassium carbonate (30.84 g, 223.1 mmol) and rac-2-bromopropanamide (8.14 g, 53.6 mmol). The reaction mixture was stirred at 90° C. for 1 h. After cooling down the reaction mixture was partitioned between water and ethyl acetate. The organic layer was separated and washed with saturated aqueous ammonium chloride solution and brine. Afterwards, the organic layer was filtrated by a water repellent filter circle (MN 617 WA) and evaporated to dryness. Water was added to the residue and the resulting suspension was dried by lyophilization to give 6.16 g (27% yield) of rac-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]-acetate.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.16 (d, J=7.86 Hz, 3H), 1.18 (t, J=6.84 Hz, 3H), 4.15 (q, J=7.10 Hz, 2H), 4.80 (s, 2H), 5.06 (q, J=6.25 Hz, 1H), 6.91 (d, J=8.87 Hz, 2H), 7.25 (s, 1H), 7.33 (t, J=8.74 Hz, 2H), 7.47 (d, J=8.87 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J=8.87, 5.07 Hz, 2H), 8.15 (br s, 2H).

LC-MS (method 2) Rt=1.09 min; MS (ESIpos): m/z=487.5 [M+H]⁺

Example 81.1 and 81.2 (R)-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate and (S)-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate Example 81.1 Ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (enantiomer 1)

HPLC separation of rac-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (6.16 g, 12.66 mmol; Example 81) on a chiral column gave 2.50 g (39% yield) of ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino.-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate, enantiomer 1.

Preparative Chiral HPLC

Labomatic HD3000, Knauer Pump 100, Labcol Vario 4000 Plus, Knauer DAD 2600; column: Amylose SB 5μ 250×50 mm Nr.34; Eluent A: hexane; Eluent B: ethanol; isocratic: 70% A+30% B; flow 150.0 mL/min; UV® 254 nm

Analytical Chiral HPLC: Rt=7.31 Min

Instrument: Agilent HPLC 1260; column: Amylose SB 3μ 100×4, 6 mm; Eluent A: hexane; Eluent B: ethanol; isocratic: 70% A+30% B; flow 1.4 mL/min; temperature: 25° C.; DAD 254 nm

Example 81.2 Ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (Enantiomer 2)

HPLC separation of rac-ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate (6.16 g, 12.66 mmol; Example 81) on a chiral column gave 2.60 g (40% yield) of ethyl 2-[4-[4-amino-2-(4-fluoro-N-[2-amino-1-methyl-2-oxo-ethyl]anilino)thiazole-5-carbonyl]phenoxy]acetate, enantiomer 2.

Preparative Chiral HPLC

Labomatic HD3000, Knauer Pump 100, Labcol Vario 4000 Plus, Knauer DAD 2600; column: Amylose SB 5μ 250×50 mm Nr.34; Eluent A: hexane; Eluent B: ethanol; isocratic: 70% A+30% B; flow 150.0 mL/min; UV® 254 nm

Analytical Chiral HPLC: Rt=10.17 Min

Instrument: Agilent HPLC 1260; column: Amylose SB 3μ 100×4, 6 mm; Eluent A: hexane; Eluent B: ethanol; isocratic: 70% A+30% B; flow 1.4 mL/min; temperature: 25° C.; DAD 254 nm

Example 82 Rac-2-(N-[4-amino-5-[4-[2-(isopropylamino)-2-oxo-ethoxy]benzoyl]thiazol-2-yl]-4-fluoro-anilino)propanamide

Rac-2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic acid (12 mg, 26 μmol, intermediate 100) and isopropylamine (3 mg, 51 μmol) were dissolved in dimethylformamide (0.4 mL). N,N-diisopropylethylamine (13 mg, 102 μmol), 4-dimethylaminopyridine (0.2 mg, 1 μmol) and HATU (19 mg, 51 μmol) were added. The reaction mixture was stirred at rt for 2 h.

The reaction mixture was filtrated and purified by RP-HPLC (method C, basic) to give 6 mg (42% yield) of the title compound.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.04-1.07 (m, 6H), 1.16 (d, J=7.35 Hz, 3H), 3.86-3.97 (m, 1H), 4.45 (s, 2H), 5.05 (q, J=6.34 Hz, 1H), 6.92 (d, J=8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J=8.87 Hz, 2H), 7.47 (d, J=8.87 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J=8.87, 5.07 Hz, 2H), 7.88 (br d, J=7.86 Hz, 1H), 8.13 (br s, 2H).

LC-MS (method 2) Rt=1.00 min; MS (ESIpos): m/z=500.6 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 7, below, using the procedure as for Example 82.

The crude product was purified by method F (individual gradient given, depending on retention time in analytical HPLC) after filtration of the reaction mixture.

TABLE 7 Examples 83-141 Example Chemical structure number Compound name Starting materials Analytics/purification/yield  83

  rac-2-(N-[4-amino-5-[4-[2- (m-tolylmethylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fIuoro- anilino)propanamide Intermediate 100, 1-(3- methylphenyl) methanamine LC-MS (method 3) Rt = 1.12 min; MS (ESIpos): m/z = 562.2 [M + H]⁺ Method F: Prep_30-60% B 11% yield  84

  rac-2-(N-[4-amino-5-[4-[2- (o-tolylmethylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(2- methylphenyl) methanamine LC-MS (method 3) Rt = 1.11 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_30-60% B 11% yield  85

  rac-2-(N-[4-amino-5-[4-[2- [(3-chlorophenyl)methylamino]- 2-oxo-ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, 1-(3- chlorophenyl) methanamine LC-MS (method 3) Rt = 1.13 min; MS (ESIpos): m/z = 582.1 [M + H]⁺ Method F: Prep_35-65% B 10% yield  86

  rac-2-(N-[4-amino-5-[4-[2- (4-methylpiperazin-1-yl)-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1- methylpiperazine LC-MS (method 3) Rt = 0.88 min; MS (ESIpos): m/z = 541.1 [M + H]⁺ Method F: Prep_20-50% B 12% yield  87

  rac-2-(N-[4-amino-5-[4-[2- (3-methylanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, m-toluidine LC-MS (method 3) Rt = 1.15 min; MS (ESIpos): m/z = 548.1 [M + H]⁺ Method F: Prep_40-70% B 12% yield  88

  rac-2-(N-[4-amino-5-[4-(2- morpholino-2-oxo- ethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, morpholine LC-MS (method 3) Rt = 0.90 min; MS (ESIpos): m/z = 528.1 [M + H]⁺ Method F: Prep_20-50% B 13% yield  89

  rac-2-(N-[4-amino-5-[4-[2-oxo-2-[2-(1- piperidyl)ethylamino]ethoxy] benzoyl]thiazol-2-yl]-4- fluoro-anilino)propanamide Intermediate 100, 2-(piperidin-1- yl)ethanamine LC-MS (method 3) Rt = 1.07 min; MS (ESIpos): m/z = 569.2 [M + H]⁺ Method F: Prep_30-60% B 5% yield  90

  rac-2-(N-[4-amino-5-[4-[2- (4-benzyl-1-piperidyl)-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 4- benzylpiperidine LC-MS (method 3) Rt = 1.26 min; MS (ESIpos): m/z = 616.2 [M + H]⁺ Method F: Prep_45-75% B 5% yield  91

  rac-2-(N-[4-amino-5-[4-[2- (2-methoxyethylamino)-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 2- methoxyethanamine LC-MS (method 3) Rt = 0.90 min; MS (ESIpos): m/z = 516.1 [M + H]⁺ Method F: Prep_20-50% B 5% yield  92

  rac-2-(N-[4-amino-5-[4-[2- (4-cyanoanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 4- aminobenzonitrile LC-MS (method 3) Rt = 1.06 min; MS (ESIpos): m/z = 559.1 [M + H]⁺ Method F: Prep_30-60% B 9% yield  93

  rac-2-(N-[4-amino-5-[4-[2- [methyl(prop-2-ynyl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N-methylprop-2- yn-1-amine LC-MS (method 3) Rt = 0.96 min; MS (ESIpos): m/z = 510.1 [M + H]⁺ Method F: Prep_25-55% B 9% yield  94

  rac-2-(N-[4-amino-5-[4-[2-[(2- methoxyphenyl) methytamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(2- methoxyphenyl) methanamine LC-MS (method 3) Rt = 1.08 min; MS (ESIpos): m/z = 578.1 [M + H]⁺ Method F: Prep_30-60% B 13% yield  95

  rac-2-(N-[4-amino-5-[4-[2-[(3- methoxyphenyl) methylamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(3- methoxyphenyl) methanamine LC-MS (method 3) Rt = 1.06 min; MS (ESIpos): m/z = 578.1 [M + H]⁺ Method F: Prep_30-60% B 10% yield  96

  rac-2-(N-[4-amino-5-[4-[2-[(2- fluorophenyl)methylamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(2- fluorophenyl) methanamine LC-MS (method 3) Rt = 1.07 min; MS (ESIpos): m/z = 566.1 [M + H]⁺ Method F: Prep_30-60% B 10% yield  97

  rac-2-(N-[4-amino-5-[4-[2-[(4- fluorophenyl)methylamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(4- fluorophenyl) methanamine LC-MS (method 3) Rt = 1.07 mirt; MS (ESIpos): m/z = 566.1 [M + H]⁺ Method F: Prep_30-60% B 13% yield  98

  rac-2-(N-[4-amino-5-[4-[2- (1H-benzimidazol-2- ylmethylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(1H- benzimidazol-2- yl)methanamine LC-MS (method 3) Rt = 0.93 min; MS (ESIpos): m/z = 588.1 [M + H]⁺ Method F: Prep_25-55% B 7% yield  99

  rac-2-(N-[4-amino-5-[4-[2- oxo-2-(2,2,2- trifluoroethylamino)ethoxy] benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 2,2,2- trifluoroethanamine LC-MS (method 3) Rt = 1.00 min; MS (ESIpos): m/z = 540.0 [M + H]⁺ Method F: Prep_25-55% B 21% yield 100

  rac-2-(N-[4-amino-5-[4-[2- [methyl(2-pyridyl)amino]-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N-methylpyridin- 2-amine LC-MS (method 3) Rt = 0.99 min; MS (ESIpos): m/z = 549.1 [M + H]⁺ Method F: Prep_25-55% B 9% yield 101

  rac-2-(N-[4-amino-5-[4-[2- [methyl-(1-methyl-4- piperidyl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, N,1- dimethylpiperidin- 4-amine LC-MS (method 3) Rt = 0.94 min; MS (ESIpos): m/z = 569.1 [M + H]⁺ Method F: Prep_25-55% B 23% yield 102

  rac-2-(N-[4-amino-5-[4-[2- (methoxyamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, O- methylhydroxylamine LC-MS (method 3) Rt = 0.90 min; MS (ESIpos): m/z = 488.1 [M + H]⁺ Method F: Prep_20-50% B 22% yield 103

  rac-2-(N-[4-amino-5-[4-[2- [(5-methylisoxazol-3- yl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, 5-methyl-1,2- oxazol-3-amine LC-MS (method 3) Rt = 0.99 min; MS (ESIpos): m/z = 539.1 [M + H]⁺ Method F: Prep_25-55% B 7% yield 104

  rac-2-(N-[4-amino-5-[4-[2- (ethylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, ethanamine LC-MS (method 3) Rt = 0.92 min; MS (ESIpos): m/z = 486.0 [M + H]⁺ Method F: Prep_25-55% B 33% yield 105

  rac-2-(N-[4-amino-5-[4-[2- (4-methylanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, p-toluidine LC-MS (method 3) Rt = 1.15 min; MS (ESIpos): m/z = 548.1 [M + H]⁺ Method F: Prep_40-70% B 37% yield 106

  rac-2-(N-[4-amino-5-[4-[2- (cyclohexylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, cyclohexanamine LC-MS (method 3) Rt = 1.12 min; MS (ESIpos): m/z = 540.1 [M + H]⁺ Method F: Prep_35-65% B 11% yield 107

  rac-3-[[2-[4-[4-amino-2-(N- [2-amino-1-methyl-2-oxo- ethyl]-4-fluoro- anilino)thiazole-5- carbonyl]phenoxy]acetyl) amino]benzamide Intermediate 100, 3- aminobenzamide LC-MS (method 3) Rt = 0.89 min; MS (ESIpos): m/z = 577.1 [M + H]⁺ Method F: Prep_20-50% B 20% yield 108

  rac-2-(N-[4-amino-5-[4-[2- oxo-2-(6- quinolylamino)ethoxy]benzoyl] thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, quinolin-6-amine LC-MS (method 3) Rt = 1.01 min; MS (ESIpos): m/z = 585.1 [M + H]⁺ Method F: Prep_25-55% B 30% yield 109

  rac-4-[[2-[4-[4-amino-2-(N- [2-amino-1-methyl-2-oxo- ethyl]-4-fluoro- anilino)thiazole-5- carbonyl]phenoxy]acetyl] amino]benzamide Intermediate 100, 4- aminobenzamide LC-MS (method 3) Rt = 1.01 mirt; MS (ESIpos): m/z = 577.1 [M + H]⁺ Method F: Prep_20-50% B 7% yield 110

  (2S)-1-[2-[4-[4-amino-2-(N- [2-amino-(1RS)-methyl-2- oxo-ethyl]-4-fluoro- anilino)thiazole-5- carbonyl]phenoxy]acetyl] pyrrolidine-2-carboxamide (mixture of two diastereomers) Intermediate 100, L-prolinamide LC-MS (method 3) Rt = 0.82 min; MS (ESIpos): m/z = 555.1 [M + H]⁺ Method F: Prep_15-45% B 15% yield 111

  rac-2-(N-[4-amino-5-[4-[2- [ethyl(methyl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, N- methylethanamine LC-MS (method 3) Rt = 0.96 mirt; MS (ESIpos): m/z = 500.1 [M + H]⁺ Method F: Prep_25-55% B 23% yield 112

  rac-2-(N-[4-amino-5-[4-[2- [(3-methylisoxazol-5- yl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100, 3-methyl-1,2- oxazol-5-amine LC-MS (method 3) Rt = 0.80 min; MS (ESIpos): m/z = 539.1 [M + H]⁺ Method F: Prep_10-40% B 4% yield 113

  rac-2-(N-[4-amino-5-[4-[2- [3-(dimethylamino)propyl- methyl-amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide intermediate 100, N,N,N′- trimethylpropane- 1,3-diamine LC-MS (method 3) Rt = 1.02 min; MS (ESIpos): m/z = 557.2 [M + H]⁺ Method F: Prep_25-55% B 12% yield 114

  rac-2-(N-[5-[4-[2-(4- acetylpiperazin-1-yl)-2-oxo- ethoxy]benzoyl]-4-amino- thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(piperazin-1- yl)ethanone LC-MS (method 3) Rt = 0.84 min; MS (ESIpos): m/z = 569 1 [M + H]⁺ Method F: Prep_15-45% B 14% yield 115

  rac-2-(N-[4-amino-5-[4-[2- oxo-2-(3- pyridylmethylamino)ethoxy] benzoyl]thiazol-2-yl]-4- fluoro-anilino)propanamide Intermediate 100, 1-(pyridin-3- yl)methanamine LC-MS (method 3) Rt = 0.90 min; MS (ESIpos): m/z = 549.1 [M + H]⁺ Method F: Prep_20-50% B 4% yield 116

  2-(N-[4-amino-5-[4-[2-(2,3- dihydroxypropylamino)-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide (mixture of stereoisomers) Intermediate 100, rac-3- aminopropane- 1,2-diol LC-MS (method 3) Rt = 0.77 mirt; MS (ESIpos): m/z = 532.0 [M + H]⁺ Method F: Prep_10-40% B 12% yield 117

  rac-2-(N-[4-amino-5-[4-[2- (4-methoxyanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]- 4-fluoro- anilino)propanamide Intermediate 100; 4-methoxyaniline LC-MS (method 3) Rt = 1.07 min; MS (ESIpos): m/z= 564.1 [M + H]⁺ Method F: Prep_30-60% B 21% yield 118

  rac-2-(N-[4-amino-5-[4-[2- [benzyl(methyl)amino]-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N-methyl-1- phenylmethanamine LC-MS (method 3) Rt = 1.10 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_30-60% B 14% yield 119

  rac-2-(N-[4-amino-5-[4-[2- (4-chloroanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 4-chloroaniline LC-MS (method 3) Rt = 1.18 min; MS (ESIpos): m/z = 568.1 [M + H]⁺ Method F: Prep_40-70% B 22% yield 120

  rac-2-(N-[4-amino-5-[4-[2-[(2- chlorophenyl)methylamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(2- chlorophenyl) methanamine LC-MS (method 3) Rt = 1.13 min; MS (ESIpos): m/z = 582.1 [M + H]⁺ Method F: Prep_35-65% B 12% yield 121

  rac-2-(N-[4-amino-5-[4-[2-[(4- chlorophenyl)methylamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(4- chlorophenyl) methanamine LC-MS (method 3) Rt = 1.13 min; MS (ESIpos): m/z = 582.1 [M + H]⁺ Method F: Prep_35-65% B 15% yield 122

  rac-2-(N-[4-amino-5-[4-[2- (4-fluoroanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 4-fluoroaniline LC-MS (method 3) Rt = 1.10 min; MS (ESIpos): m/z = 552.1 [M + H]⁺ Method F: Prep_30-60% B 24% yield 123

  rac-2-(N-[4-amino-5-[4-[2- (azepan-1-yl)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, azepane LC-MS (method 3) Rt = 1.08 min; MS (ESIpos): m/z = 540.1 [M + H]⁺ Method F: Prep_30-60% B 6% yield 124

  rac-2-(N-[4-amino-5-[4-[2-[(4- methoxyphenyl)methylamino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(4- methoxyphenyl) methanamine LC-MS (method 3) Rt = 1.05 min; MS (ESIpos): m/z = 578.1 [M + H]⁺ Method F: Prep_30-60% B 8% yield 125

  2-(N-[4-amino-5-[4-[2-oxo- 2-(1-phenylethylamino)ethoxy] benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (mixture of stereoisomers) Intermediate 100, rac-1- phenylethanamine LC-MS (method 3) Rt = 1.10 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_30-60% B 9% yield 126

  rac-2-(N-[4-amino-5-[4-[2-oxo-2-(p- tolylmethylamino)ethoxy] benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100: 1-(4- methylphenyl) methanamine LC-MS (method 3) Rt = 1.12 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_35-65% B 13% yield 127

  rac-2-(N-[4-amino-5-[4-[2-[methyl(2- phenylethyl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100: N-methyl-2- phenylethanamine LC-MS (method 3) Rt = 1.11 min; MS (ESIpos): m/z = 576.1 [M + H]⁺ Method F: Prep_30-60% B 23% yield 128

  2-(N-[4-amino-5-[4-[2-(3- methyl-1-piperidyl)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (mixture of stereoisomers) Intermediate 100, rac-3- methylpiperidine LC-MS (method 3) Rt = 1.11 min; MS (ESIpos): m/z = 540.1 [M + H]⁺ Method F: Prep_30-60% B 4% yield 129

  rac-2-(N-[4-amino-5-[4-[2- (4-methyl-1-piperidyl)-2- oxo-ethoxy]benzoyl]thiazol- 2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 4- methylpiperidine LC-MS (method 3) Rt = 1.11 min; MS (ESIpos): m/z = 540.1 [M + H]⁺ Method F: Prep_30-60% B 5% yield 130

  rac-2-(N-[5-[4-[2-(4- acetamidoanilino)-2-oxo- ethoxy]benzoyl]-4-amino- thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N-(4- aminophenyl) acetamide LC-MS (method 3) Rt = 0.92 min; MS (ESIpos): m/z = 591.1 [M + H]⁺ Method F. Prep_25-55% B 25% yield 131

  rac-2-(N-[4-amino-5-[4-[2- oxo-2-(1H-pyrazolo[3,4- d]pyrimidin-4- ylamino)ethoxy]benzoyl] thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1H-pyrazolo[3,4- d]pyrimidin-4- amine LC-MS (method 3) Rt = 0.80 min; MS (ESIpos): m/z = 576.1 [M + H]⁺ Method F: Prep_10-40% B 7% yield 132

  rac-2-(N-[4-amino-5-[4-[2- (cyclopentylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, cyclopentanamine LC-MS (method 3) Rt = 1.06 min; MS (ESIpos): m/z = 526.1 [M + H]⁺ Method F. Prep_30-60% B 8% yield 133

  rac-2-(N-[4-amino-5-[4-[2- (3,4-dihydro-1H-isoquinolin- 2-yl)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1,2,3,4- tetrahydroisoquinoline LC-MS (method 3) Rt = 1.13 min; MS (ESIpos): m/z = 574.1 [M + H]⁺ Method F: Prep_35-65% B 8% yield 134

  rac-2-(N-[4-amino-5-[4-(2- isoindolin-2-yl-2-oxo- ethoxy)benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, isoindoline LC-MS (method 3) Rt = 1.09 min; MS (ESIpos): m/z = 560.1 [M + H]⁺ Method F: Prep_30-60% B 6% yield 135

  rac-2-(N-[4-amino-5-[4-[2-[2- furylmethyl(methyl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 1-(2-furyl)-N- methylmethanamine LC-MS (method 3) Rt = 1.04 min; MS (ESIpos): m/z = 552.1 [M + H]⁺ Method F: Prep_30-60% B 12% yield 136

  rac-2-(N-[4-amino-5-[4-[2- [4-(dimethylamino)-1- piperidyl]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N,N- dimethylpiperidin- 4-amine LC-MS (method 3) Rt = 0.97 min; MS (ESIpos): m/z = 569.2 [M + H]⁺ Method F: Prep_25-55% B 13% yield 137

  rac-2-(N-[4-amino-5-[4-[2- [methyl(3- pyridylmethyl)amino]-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N-methyl-1- (pyridin-3- yl)methanamine LC-MS (method 3) Rt = 0.92 min; MS (ESIpos): m/z = 563.1 [M + H]⁺ Method F: Prep_25-55% B 24% yield 138

  rac-2-(N-[4-amino-5-[4-[2- (N,2-dimethylanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100; N,2- dimethylaniline LC-MS (method 3) Rt = 1.13 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_35-65% B 13% yield 139

  rac-2-(N-[4-amino-5-[4-[2- (N,4-dimethylanilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N.4- dimethylaniline LC-MS (method 3) Rt = 1.16 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_40-70% B 26% yield 140

  rac-2-(N-[4-amino-5-[4-[2- (N,3-dimethyianilino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, N,3- dimethylaniline LC-MS (method 3) Rt = 1.15 min; MS (ESIpos): m/z = 562.1 [M + H]⁺ Method F: Prep_40-70% B 31% yield 141

  rac-2-(N-[4-amino-5-[4-[2- (2,2-dimethylpropylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- anilino)propanamide Intermediate 100, 2,2- dimethylpropan- 1-amine LC-MS (method 3) Rt = 1.08 mirt; MS (ESIpos): m/z = 528.1 [M + H]⁺ Method F: Prep_30-60% B 4% yield

Example 142 2-(N-[5-[4-[2-(1-adamantylamino)-2-oxo-ethoxy]benzoyl]-4-amino-thiazol-2-yl]-4-fluoro-anilino)propanamide (Single Stereoisomer)

2-[4-[4-amino-2-(N-[2-amino-1-methyl-2-oxo-ethyl]-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]acetic acid (enantiomer 2) (30 mg, 65 μmol; Intermediate 100.2) and adamantan-1-amine (20 mg, 131 μmol) were dissolved in dimethylformamide (0.5 mL). N,N-diisopropylethylamine (25 mg, 196 μmol), 4-dimethylaminopyridine (0.4 mg, 3 μmol) and HATU (50 mg, 131 μmol) were added. The reaction mixture was stirred overnight at rt.

The reaction mixture was filtrated and purified by RP-HPLC (method E, basic) to give 12 mg (29% yield) of the title compound.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.16 (d, J=7.60 Hz, 3H), 1.59-1.63 (m, 6H), 1.92 (d, J=2.53 Hz, 6H), 2.00 (br d, J=1.77 Hz, 3H), 4.41 (s, 2H), 5.00-5.13 (m, 1H), 6.89 (d, J=8.87 Hz, 2H), 7.24 (s, 1H), 7.30-7.36 (m, 3H), 7.46 (d, J=8.87 Hz, 2H), 7.57 (s, 1H), 7.64 (dd, J=9.00, 5.20 Hz, 2H), 8.11 (br s, 2H).

LC-MS (method 2) Rt=1.32 min; MS (ESIpos): m/z=592.5 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 8, below, using the procedure as for Example 142.

TABLE 8 Examples 143-155 Example Chemical structure Starting number Compound name materials Analytics/purification/yield 143

Intermediate 100.2; 1- adamantyl- methanamine ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 1.31 (d, J = 2.03 Hz, 6H), 1.44-1.48 (m, 3H), 1.55-1.61 (m, 3H), 1.80 (br s, 3H), 2.78 (d, J = 6.59 Hz, 2H), 4.54 (s, 2H), 5.02-5.11 (m, 1H), 6.94 (d, J = 8.87 Hz, 2H), 7.25 (s, 1H), 7.32 (t, J = 8.74 Hz, 2H), 7.48 (d, J = 8.62 Hz, 2H), 7.57 (s, 1H), 7.63 (dd, J = 8.87, 4.82 Hz, 2H), 7.82 (t, J = 6.21 Hz, 1H), 8.19 (br s, 2H). LC-MS (method 1) Rt = 1.29 min; MS (ESIpos): m/z = 606.5 [M + H]⁺ RP-HPLC (method E, basic) 41% yield 144

Intermediate 100.2; 2-(1- adamantyl) ethanamine ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1 13-1.17 (m, 3H), 1.17-1.24 (m, 2H), 1.44 (d, J = 2.03 Hz, 6H), 1.55-1.61 (m, 3H), 1.62-1.68 (m, 3H), 1.87-1.91 (m, 3H), 3.07-3.15 (m, 2H), 4.45 (s, 2H), 5.01-5.10 (m, 1H), 6.92 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J = 8.87 Hz, 2H), 7.47 (d, J = 8.87 Hz, 2H), 7.57 (s, 1H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 8.30 (br s, 2H), 7.97 (t, J = 5.83 Hz, 1H). LC-MS (method 1) Rt = 1.40 min; MS (ESIpos): m/z = 620.6 [M + H]⁺ RP-HPLC (method E, basic) 145

Intermediate 100.2; 4- chloroaniline ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 4.73 (s, 2H), 5.00-5.11 (m, 1H), 6.98 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J = 8.87 Hz, 2H), 7.38 (d, J = 8.87 Hz, 2H), 7.49 (d, J = 8.87 Hz, 2H), 7.57 (s, 1H), 7.61-7.67 (m, 4H), 8.08 (br s, 2H), 10.24 (s, 1H). LC-MS (method 1) Rt = 1.20 min; MS (ESIpos): m/z = 568.4 [M + H]⁺ RP-HPLC (method D, basic) 59% yield 146

Intermediate 100.2; 4- amino- benzamide ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 4.76 (s, 2H), 5.01-5.09 (m, 1H), 6.99 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.26 (br s, 1H), 7.33 (t, J = 8.74 Hz, 2H), 7.49 (d, J = 8.62 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J = 9.00, 5.20 Hz, 2H), 7.68 (d, J = 8.87 Hz, 2H), 7.84 (d, J = 8.87 Hz, 2H), 7.87-7.90 (m, 1H), 8.10 (br s, 2H), 10.31 (s, 1H). LC-MS (method 1) Rt = 0.88 min; MS (ESIpos): m/z = 577.4 [M + H]⁺ RP-HPLC (method C, basic) 48% yield 147

Intermediate 100.2; rac-3- amino- propane-1,2- diol ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 2.99-3.08 (m, 1H), 3.24-3.30 (m, 4H), 3.48-3.56 (m, 2H), 4.49-4.53 (m, 2H), 4.55-4.60 (m, 1H), 4.75-4.85 (m, 1H), 5.05 (br d, J = 7.10 Hz, 1H), 6.92-6.96 (m, 2H), 7.24 (s, 1H), 7.33 (t, J = 8.87 Hz, 2H), 7.48 (d, J = 8.62 Hz, 2H), 7.58 (s, 1H), 7.61-7.67 (m, 2H), 7.93-7.99 (m, 1H). LC-MS (method 1) Rt = 0.78 min; MS (ESIpos): m/z = 532.4 [M + H]⁺ RP-HPLC (method B, basic) 54% yield 148

Intermediate 100.2; 2- (piperidin-1- yl) ethanamine (salt with hydrogen chloride) ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3H), 1.28-1.46 (m, 7H), 2.22-2.30 (m, 6H), 3.16-3.24 (m, 2H), 4.42-4.56 (m, 2H), 4.96-5.19 (m, 1H), 6.90-6.95 (m, 2H), 7.23-7.26 (m, 1H), 7.33 (t, J = 8.74 Hz, 2H), 7.45-7.50 (m, 2H), 7.56-7.59 (m, 1H), 7.61-7.66 (m, 2H), 7.87 (t, J = 5.32 Hz, 1H). LC-MS (method 1) Rt = 0.77 min; MS (ESIpos): m/z = 569.5 [M + H]⁺ RP-HPLC (method C, basic) 54% yield 149

Intermediate 100.2; ammonia in dioxane ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 4.44 (s, 2H), 5.05 (q, J = 6.84 Hz, 1H), 6.92 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J = 8.87 Hz, 2H), 7.38 (br s, 1H), 7.47 (d, J = 8.87 Hz, 2H), 7.52 (br s, 1H), 7.58 (s, 1H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 8.07 (br s, 2H). LC-MS (method 1) Rt = 0.82 min; MS (ESIpos): m/z = 458.3 [M + H]⁺ RP-HPLC (method B, basic) 60% yield 150 and (R)-2-(N-[4-amino-5-[4-[2-(methylamino)-2-oxo-ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- 151 anilino)propanamide and (S)-2-(N-[4-amino-5-[4-[2-(methylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro-anilino)propanamide 150

Intermediate 100.1; methanamine in tetrahydro- furane 2.0M ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3H), 2.63 (d, J = 4.82 Hz, 3H), 4.47 (s, 2H), 5.05 (q, J = 6.00 Hz, 1H), 6.93 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J = 8.74 Hz, 2H), 7.48 (d, J = 8.62 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 8.15 (br s, 2H), 8.00-8.04 (m, 1H). LC-MS (method 1) Rt = 0.88 min; MS (ESIpos): m/z = 472.3 [M + H]⁺ RP-HPLC (method C, basic) 64% yield 151

Intermediate 100.2; methanamine in tetrahydro- furane 2.0M ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.60 Hz, 3H), 2.63 (d, J = 4.82 Hz, 3H), 4.47 (s, 2H), 5.05 (q, J = 7.35 Hz, 1H), 6.93 (d, J = 8.87 Hz, 2H), 7.25 (s, 1H), 7.33 (t, J = 8.74 Hz, 2H), 7.48 (d, J = 8.62 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 8.07 (br s, 2H), 7.99-8.05 (m, 1H). LC-MS (method 1) Rt = 0.88 min; MS (ESIpos): m/z = 472.3 [M + H]⁺ RP-HPLC (method C, basic) 29% yield 152 and (R)-2-(N-[4-amino-5-[4-[2-(isopropylamino)-2-oxo-ethoxy]benzoyl]thiazol-2-yl]-4-fluoro- 153 anilino)propanamide and (S)-2-(N-[4-amino-5-[4-[2-(isopropylamino)-2-oxo- ethoxy]benzoyl]thiazol-2-yl]-4-fluoro-anilino)propanamide 152

Intermediate 100.1; propan-2- amine ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.04-1.08 (m, 6H), 1.16 (d, J = 7.35 Hz, 3H), 3.86-3.96 (m, 1H), 4.45 (s, 2H), 5.05 (br q, J = 7.10 Hz, 1H), 6.92 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J = 8.87 Hz, 2H), 7.47 (d, J = 8.87 Hz, 2H), 7.57 (s, 1H), 7.64 (dd, J = 9.00, 5.20 Hz, 2H), 8.08 (br s, 2H), 7.87 (br d, J = 8.11 Hz, 1H). LC-MS (method 1) Rt = 1.00 min; MS (ESIpos): m/z = 500.4 [M + H]⁺ RP-HPLC (method C, basic) 75% yield 153

Intermediate 100.2; propan-2- amine ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.06 (d, J = 6.59 Hz, 6H), 1.16 (d, J = 7.35 Hz, 3H), 3.87-3.96 (m, 1H), 4.45 (s, 2H), 5.05 (q, J = 7.86 Hz, 1H), 6.92 (d, J = 8.87 Hz, 2H), 7.24 (s, 1H), 7.33 (t, J = 8. 87 Hz, 2H), 7.47 (d, J = 8.87 Hz, 2H), 7.57 (s, 1H), 7.64 (dd, J = 9.00, 5.20 Hz, 2H), 8.06 (br s, 2H), 7.87 (br d, J = 7.86 Hz, 1H). LC-MS (method 1) Rt = 1.00 min; MS (ESIpos): m/z = 500.4 [M + H]⁺ RP-HPLC (method C, basic) 72% yield 154

Intermediate 82; propan-2- amine ¹H-NMR (400 MHz, DMSO-d₆): δ ppm = 0.94 (d, J = 6.59 Hz, 6H), 1.16 (d, J = 7.35 Hz, 3H), 1.43 (s, 6H), 3.83-3.96 (m, 1H), 4.99-5.11 (m, 1H), 6.79 (d, J = 8.62 Hz, 2H), 7.24 (s, 1H), 7.32 (t, J = 8 87 Hz, 2H), 7.42 (d, J = 8.62 Hz, 2H), 7.57 (s, 1H), 7.63 (dd, J = 8.74, 5.20 Hz, 2H), 7.75 (d, J = 8.11 Hz, 1H), 7.80-8.50 (m, 2H). LC-MS (method 1) Rt = 1.10 min; MS (ESIpos): m/z = 528.4 [M + H]⁺ RP-HPLC (method D, basic) 23% yield 155

Intermediate 82; ammonia ¹H-NMR (400 MHz, DMSO-d6): δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 1.43 (s, 6H), 4.99-5.11 (m, 1H), 6.82 (d, J = 8.87 Hz, 2H), 7.25 (br d, J = 7.86 Hz, 2H), 7.33 (t, J = 8.87 Hz, 2H), 7.44 (d, J = 8.62 Hz, 2H), 7.50-7.52 (m, 1H), 7.57-7.59 (m, 1H), 7.64 (dd, J = 8.74, 5.20 Hz, 2H), 7.82-8.34 (m, 2H). LC-MS (method 1) Rt = 0.93 min; MS (ESIpos): m/z = 486.3 [M + H]⁺ RP-HPLC (method C, basic) 42% yield

Example 156 Rac-2-(N-(5-benzoyl-4-methyl-thiazol-2-yl)-4-fluoro-anilino)propanamide

[2-(4-fluoroanilino)-4-methyl-thiazol-5-yl]-phenyl-methanone (40 mg, 0.13 mmol; Intermediate 81) was dissolved in N,N-dimethylformamide (1.4 mL), followed by the addition of potassium carbonate (177 mg, 1.28 mmol) and rac-2-bromopropanamide (97 mg, 0.64 mmol). The reaction mixture was stirred at 90° C. for 1 h. The reaction mixture was filtrated and purified by RP-HPLC (method D, basic) to give 24 mg (48% yield) of the title compound.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.16 (d, J=7.35 Hz, 3H), 2.24-2.27 (m, 3H), 5.11 (q, J=7.35 Hz, 1H), 7.20 (s, 1H), 736 (t, J=8.87 Hz, 2H), 743-7.48 (m, 2H), 7.51-7.57 (m, 3H), 7.62 (s, 1H), 7.67 (dd, J=9.13, 5.07 Hz, 2H).

LC-MS (method 2) Rt=1.16 min; MS (ESIpos): m/z=384.4 [M+H]⁺

Example 157 Rac-2-(N-[4-amino-5-(4-hydroxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide

Rac-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (100 mg, 204 μmol, Example 158) was dissolved in ethanol (2.5 mL). Under nitrogen atmosphere was added palladium on carbon (325 mg, 10% purity, 306 μmol; CAS-RN 7440-05-3) and then the nitrogen atmosphere was evacuated and was replaced with hydrogen. The mixture was stirred for 3.5 h at rt under hydrogen atmosphere. Further palladium on carbon (100 μmol) was added and the mixture was stirred for a further 3 h at rt under hydrogen atmosphere. The reaction mixture was filtered over celite, washed with ethanol and the filtrate was concentrated under reduced pressure. The residue was purified by RP-HPLC (method B, basic) to give 27 mg (31% yield) of the title compound.

LC-MS (method 2) Rt=0.69 min; MS (ESIpos): m/z=401.2 [M+H]⁺

¹H-NMR (400 MHz, DMSO-d6): δ ppm=116 (d, J=7.35 Hz, 3H), 2.08 (s, 1H), 5.01-5.09 (m, 1H), 6.70-6.75 (m, 2H). 7.24 (s, 1H), 7.33 (t, J=8.87 Hz, 2H), 737-7.41 (m, 2H), 7.57 (s, 1H), 7.64 (dd, J=9.00, 4.94 Hz, 2H), 7.76-8.21 (m, 2H), 9.90 (br s, 1H)

Example 158 Rac-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide

[4-amino-2-(4-fluoroanilino)-1,3-thiazol-5-yl][4-(benzyloxy)phenyl]methanone (610 mg, 1.45 mmol, Intermediate 77) was suspended in DMF (10 mL), rac-2-bromopropanamide (265 mg, 1.75 mmol) and potassium carbonate (301 mg, 2.18 mmol) were added. The reaction mixture was stirred for 3.5 h at rt. Further rac-2-bromopropanamide (265 mg, 1.75 mmol) and potassium carbonate (301 mg, 2.18 mmol) were added and the mixture was stirred overnight at rt. The mixture was treated with water and stirred for 30 min. The resulting precipitate was isolated by filtration, washed with water and dried to give 641 mg (98% purity, 88% yield) of the title compound.

¹H-NMR (400 MHz, DMSO-d6): δ ppm=1.16 (d, J=7.35 Hz, 3H), 5.02-5.09 (m, 1H), 5.11 (s, 2H), 7.00 (d, J=8.87 Hz, 2H), 7.25 (s, 1H), 7.31-7.45 (m, 7H), 7.46-7.50 (m, 2H), 7.57 (s, 1H), 7.64 (dd, J=8.87, 5.07 Hz, 2H), 7.78-8.38 (m, 2H).

LC-MS (method 2) Rt=1.25 min; MS (ESIpos): m/z=491.3 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 9, below, using the procedure as for Example 1

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) after precipitation, extraction or filtration of the reaction mixture if necessary.

Enantiomers were separated from their racemate by chiral HPLC using the column and solvent conditions stated.

TABLE 9 Example 159-256 Exam- ple num- Chemical structure Starting ber Compound name materials Analytics/purification/yield 159

Intermediate 101; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.04-5.08 (m, 1H), 6.94 (t, J = 60 Hz, 1H), 7.29-7.33 (m, 3H), 7.60-7.65 (m, 3H), 7.72 (d, J = 8.11 Hz, 1H), 8.04 (dd, J = 7.98, 2.15 Hz, 1H), 8.30 8.34 (m, 2H), 8.76 (d, J = 1.52 Hz, 1H). RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.00 min MS (ESIpos): m/z = 436.4 [M + H]⁺ 48% yield 160

Intermediate 102; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 3H), 5.00-5.04 (m, 1H), 7.31 (s, 1H), 7.40-7.44 (m, 2H), 7.55-7.58 (m, 1H), 7.63-7.67 (m, 2H), 7.91 (dd, J = 6.84, 2.28 Hz, 1H), 8.27-8.31 (m, 2H), 8.60-8.64 (m, 2H). Isolated via precipitation LC-MS (method 2) Rt = 0.94 min MS (ESIpos): m/z = 420.3 [M + H]⁺ 60% yield 160.1 (R)-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3-chloro-4-fluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2- 160.2 yl]-3-chloro-4-fluoro-anilino)propanamide 160.1

Example 160 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 4H), 5.00-5.05 (m, 1H), 7.31 (s, 1H), 7.42 (d, J = 6.08 Hz, 2H), 7.57 (m, 1H), 7.62-7.67 (m, 2H), 7.92 (dd, J = 6.84, 2.53 Hz, 1H), 8.25-8.29 (m, 2H), 8.63 (d, J = 5.83 Hz, 2H). LC-MS (method 1) Rt = 0.86 min; MS (ESIpos): m/z = 420.3 [M + H]⁺ 34% yield Chiral HPLC Example 160.1 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3-chloro-4-fluoro-anilino)propanamide (560 mg, 1.33 mmol, Example 160) on a chiral column gave 190 mg (34% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3-chloro-4-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC PrepCon Labomatic HPLC; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 120 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.23 min Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 160.2

Example 160 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 4H), 5.00-5.04 (m, 1H), 7.31 (s, 1H), 7.42 (d, J = 6.08 Hz, 2H), 7.55-7.59 (m, 1H), 7.63-7.67 (m, 2H), 7.92 (dd, J = 6.84, 2.53 Hz, 1H), 8.24-8.30 (m, 2H), 8.63 (d, J = 5.83 Hz, 2H). LC-MS (method 1) Rt = 0.86 min; MS (ESIpos): m/z = 420.3 [M + H]⁺ 33% yield Chiral HPLC Example 160.2 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3-chloro-4-fluoro-anilino)propanamide (560 mg, 1.33 mmol, Example 160) on a chiral column gave 185 mg (33% yield) of 2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-3-chloro-4-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC PrepCon Labomatic HPLC; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 120 mL/min; temperature: 25° C. UV: 254 nm Analytical chiral HPLC: Rt = 1.67 min Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 161

Intermediate 103; rac-2- bromo- propanamide ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.57 Hz, 3H), 3.76 (s, 3H), 5.00-5.04 (m, 1H), 6.94 (d, J = 8.83 Hz, 2H), 7.29 (s, 1H), 7.50 (d, J = 8.83 Hz, 2H), 7.57-7.59 (m, 1H), 7.63 (s, 1H), 7.64-7.68 (m, 1H), 7.92(m, 1H), 8.12-8.17 (m, 2H). LC-MS (method 2) Rt = 1.17 min Isolated via precipitation MS (ESIpos): m/z = 449.3 [M + H]⁺ 70% yield 161.1 (R)-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3-chloro-4-fluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2- 161.2 yl]-3-chloro-4-fluoro-anilino)propanamide 161.1

Example 161 ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.57 Hz, 3H), 3.76 (s, 3H), 5.00-5.04 (m, 1H), 6.94 (d, J = 8.83 Hz, 2H), 7.29 (s, 1H), 7.50 (d, J = 8.83 Hz, 2H), 7.55-7.59 (m, 1H), 7.63 (s, 1H), 7.64-7.66 (m, 1H), 7.90-7.94 (m, 1H), 8.14 (m, 2H). LC-MS (method 1) Rt = 1.13 min MS (ESIpos): m/z = 449.4 [M + H]⁺ [α]_(D) ²⁰ = 85° c = 10.3 mg/mL in DMSO 31% yield Chiral HPLC Example 161.1 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3-chloro-4-fluoro-anilino)propanamide (1300 mg, 2.9 mmol, Example 161) on a chiral column followed by trituration in MTBE gave 401 mg (31% yield) of 2-(N-[4-amino-5-(4- methoxybenzoyl)thiazol-2-yl]-3-chloro-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.11 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% B + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 161.2

Example 161 ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.57 Hz, 3H), 3.76 (s, 3H), 5.00-5.04 (m, 1H), 6.94 (d, J = 8.83 Hz, 2H), 7.29 (s, 1H), 7.50 (d, J = 8.83 Hz, 2H), 7.58 (m, 1H), 7.63 (s, 1H), 7.66 (m, 1H), 7.90-7.94 (m, 1H), 8.10-8.16 (m, 2H). LC-MS (method 1) Rt = 1.13 min; MS (ESIpos): m/z = 449.4 [M + H]⁺ [α]_(D) ²⁰ = −89° c = 10.1 mg/mL in DMSO 29% yield Chiral HPLC Example 161.2 HPLC separation of rac-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-3-chloro-4-fluoro-anilino)propanamide (1300 mg, 2.9 mmol, Example 161) on a chiral column followed by trituration in MTBE gave 375 mg (29% yield) of 2-(N-[4-amino-5-(4- methoxybenzoyl)thiazol-2-yl]-3-chloro-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.48 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 162

Intermediate 104; rac-2- bromo- propanamide ¹H-NMR (400 MHz, DMSO-d₆): δ ppm = 1.13-1.20 (m, 3H), 5.00-5.12 (m, 1H), 7.10 (d, J = 8.62 Hz, 1H), 7.23-7.28 (m, 1H), 7.34 (t, J = 8.87 Hz, 2H), 7.52-7.68 (m, 3H), 7.97-8.03 (m, 1H), 8.06-8.35 (m, 1H), 8.35-8.42 (m, 1H). LC-MS (method 2) Rt = 1.10 min Isolated via precipitation MS (ESIpos): m/z = 452.2 [M + H]⁺ 61% yield 162.1 (R)-2-(N-[4-amino-5-(6-(difluoromethoxy)pyridine-3-carbonyl]thiazol-2-yl]-4- and fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-[6- 162.2 (difluoromethoxy)pyridine-3-carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 162.1

Example 162 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.06 (m, 1H), 7.11 (d, J = 8.62 Hz, 1H), 7.26 (s, 1H), 7.34 (m, 2H), 7.59 (s, 1H), 7.65 (br d, J = 3.80 Hz, 2H), 7.71 (t, J = 72 Hz, 1H), 8.00 (dd, J = 8.49, 2.41 Hz, 1H), 8.19 (m, 1H), 8.38 (d, J = 2.03 Hz, 1H), 8.38 (m, 1H). LC-MS (method 2) Rt = 1.13 min; MS (ESIpos): m/z = 452.3 [M + H]⁺ [α]_(D) ²⁰ = −71° c = 8 mg/mL in DMSO 37% yield Chiral HPLC Example 162.1 HPLC separation of rac-2-(N-[4-amino-5-[6-(difluoroethoxy)pyridine-3-carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (105 mg, 0.23 mmol, Example 162) on a chiral column gave 39 mg (37% yield) of 2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.07 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 162.2

Example 162 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.02-5.08 (m, 1H), 7.11 (d, J = 8.62 Hz, 1H), 7.26 (s, 1H), 7.34 (m, 2H), 7.59 (s, 1H), 7.65 (br d, W3.80 Hz, 2H), 7.71 (t, J = 72 Hz, 1H), 8.00 (dd, J = 8.49, 2.41 Hz, 1H), 8.17-8.21 (m, 1H), 8.38 (d, J = 2.03 Hz, 1H), 8.38 (m, 1H). LC-MS (method 2) Rt = 1.13 min; MS (ESIpos): m/z = 452.3 [M + H]⁺ [α]_(D) ²⁰ = 74° c = 8.7 mg/mL in DMSO 39% yield Chiral HPLC Example 162.2 HPLC separation of rac-2-(N-[4-amino-5-[6-(difluoroethoxy)pyridine-3-carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (105 mg, 0.23 mmol, Example 162) on a chiral column gave 41 mg (39% yield) of 2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.40 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 70% A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 163

Intermediate 105; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, = 8 Hz, 3H), 5.03 (q, J = 6.84 Hz, 1H), 7.09-7.12 (m, 1H), 7.30 (s, 1H), 7.58 (m, 3H), 7.71 (t, W72 Hz, 1H), 7.79 (m, 1H), 8.02 (dd, J = 8.49, 2.41 Hz, 1H), 8.19-8.23 (m, 2H), 8.41 (d, J = 2.03 Hz, 1H). LC-MS (method 2) Rt = 1.15 min Biotage (method X) MS (ESIpos): m/z = 470.3 [M + H]⁺ 56% yield 163.1 (R)-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3-carbonyl]thiazol-2-yl]-3,4- and difluoro-anilino)propanamide and (S)-(N-[4-amino-5-[6- 163.2 (difluoromethoxy)pyridine-3-carbonyl]thiazol-2-yl]-3,4-difluoro- anilino)propanamide 163.1

Example 163 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.02-5.06 (m, 1H), 7.11 (d, J = 8.62 Hz, 1H), 7.26 (s, 1H), 7.34 (m, 2H), 7.59 (s, 1H), 7.65 (br d, J = 3.80 Hz, 2H), 7.71 (t, J = 72 Hz, 1H), 8.00 (dd, J = 8.49, 2.41 Hz, 1H), 8.17-8.22 (m, 1H), 8.38 (d, J = 2.03 Hz, 1H), 8.35-8.37 (m, 1H). LC-MS (method 2) Rt = 1.15 min 41% yield Chiral HPLC Example 163.1 HPLC separation of rac-2-(N-[4-amino-5-[6-(difluoroethoxy)pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide (120 mg, 0.26 mmol, Example 163) on a chiral column gave 49 mg (41% yield) of 2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3,4-difluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-2; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 2.42 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 163.2

Example 163 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3H), 5.02-5.06 (m, 1H), 7.12 (d, J = 8.62 Hz, 1H), 7.30 (s, 1H), 7.48-7.52 (m, 1H), 7.58-7.62 (m, 2H), 7.72 (t, J = 72 Hz, 1H), 7.77-7.82 (m, 1H), 8.02 (dd, J = 8.49, 2.41 Hz, 1H), 8.20-8.25 (m, 2H), 8.41 (d, J = 2.53 Hz, 1H). LC-MS (method 2) Rt = 1.15 min 56% yield Chiral HPLC Example 163.2 HPLC separation of rac-2-(N-[4-amino-5-[6-(difluoroethoxy)pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide (120 mg, 0.26 mmol, Example 163) on a chiral column gave 68 mg (56% yield) of 2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-carbonyl]thiazol- 2-yl]-3,4-difluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-2; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 3.72 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 164

Intermediate 106, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 8.11 Hz, 3H), 5.01-5.05 (m, 1H), 7.12 (d, J = 8.62 Hz, 1H), 7.32 (s, 1H), 7.53 (m, 1H), 7.64 (s, 1H), 7.71 (t, J = 72 Hz, 1H), 7.72-7.76 (m, 2H), 8.03 (dd, J = 8 62, 2.53 Hz, 1H), 8.20-8.25 (m, 2H), 8.41 (d, J = 2.28 Hz, 1H). Biotage (method X) LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 486.3 [M + H]⁺ 53% yield 164.1 (R)-2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3-carbonyl]thiazol-2-yl]-4- and chloro-3-fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-[6- 164.2 (difluoromethoxy)pyridine-3-carbonyl]thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide 164.1

Example 164 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 5.01-5.05 (m, 1H), 7.12 (m, 1H), 7.32 (s, 1H), 7.53 (m. 1H), 7.64 (s, 1H), 7.71 (t, J = 72 Hz, 1H), 7.72-7.77 (m, 2H), 8.03 (dd, J = 8.62, 2.53 Hz, 1H), 8.21-8.26 (m, 2H), 8.39-8.42 (m, 1H). 45% yield Chiral HPLC Example 164.1 HPLC separation of rac-2-(N-[4-amino-5-[6-(difluoroethoxy)pyridine-3-carbonyl)thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide (113 mg, 0.23 mmol, Example 164) on a chiral column gave 51 mg (45% yield) of 2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3- carbonyl]thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-2; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 2.6 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 164.2

Example 164 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 5.01-5.05 (m, 1H), 7.10-7.14 (m, 1H), 7.32 (s, 1H), 7.50-7.56 (m, 1H), 7.64 (s, 1H), 7.71 (t, J = 72 Hz, 1H), 7.70-7.78 (m, 2H), 8.03 (dd, J = 8.62, 2.53 Hz, 1H), 8.20-8.25 (m, 2H), 8.40-8.43 (m, 1H). 45% yield Chiral HPLC Example 164.2 HPLC separation of rac-2-(N-[4-amino-5-[6-(difluoroethoxy)pyridine-3-carbonyl)thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide (113 mg, 0.23 mmol, Example 164) on a chiral column gave 51 mg (45% yield) of 2-(N-[4-amino-5-[6-(difluoromethoxy)pyridine-3- carbonyl]thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-2; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 3.87 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 95% A + 5% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 165

Intermediate 107, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 3H), 5.00-5.04 (m, 1H), 5.12 (s, 2H), 7.00-7.05 (m, 2H), 7.24-7.64 (m, 11H), 7.77-7.81 (m, 1H), 8.03-8.07 (m, 2H). Biotage (method X) LC-MS (method 2) Rt = 1.32 min MS (ESIpos): m/z = 509.4 [M + H]⁺ 57% yield 165.1 (R)-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2-yl-3,4-difluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2- 165.2 yl]-3,4-difluoro-anilino)propanamide 165.1

Example 165 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 4H), 5.00-5.04 (m, 1H), 5.12 (s, 2H), 7.00-7.05 (m, 2H), 7.28 (s, 1H), 7.36-7.44 (m, 5H), 7.47-7.52 (m, 3H), 7.59-7.63 (m, 2H), 7.78 (ddd, J = 11.34, 7.54, 2.41 Hz, 1H), 8.02-8.06 (m, 2H) 46% yield Chiral HPLC Example 165.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(benzyloxylbenzoyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide (340 mg, 0.67 mmol, Example 165) on a chiral column gave 155 mg (46% yield) of 2-(N-[4-amino-5-(4-(benzylozybenzoyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 1.73 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 165.2

Example 165 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 4H), 5.00-5.04 (m, 1H), 5.12 (s, 2H), 7.02 (m, 2H), 7.28 (s, 1H), 7.35-7.45 (m, 5H), 7.47-7.52 (m, 3H), 7.58-7.62 (m, 2H), 7.78 (ddd, J = 11.34, 7.54, 2.41 Hz, 1H), 8.02-8.05 (m, 2H) 46% yield Chiral HPLC Example 165.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(benzyloxylbenzoyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide (340 mg, 0.67 mmol, Example 165) on a chiral column gave 156 mg (46% yield) of 2-(N-[4-amino-5-(4-(benzylozybenzoyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 2.32 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 166

Intermediate 108, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 5.00-5.04 (m, 1H), 5.12 (s, 2H), 7.00-7.05 (m, 2H), 7.30-7.42 (m, 6H), 7.47-7.52 (m, 3H), 7.63 (s, 1H), 7.73-7.77 (m, 2H), 8.09-8.13 (m, 2H) Biotage (method X) LC-MS (method 2) Rt = 1.36 min MS (ESIpos): m/z = 525.3 [M + H]⁺ 57% yield 166.1 (R)-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2- 166.2 yl]-4-chloro-3-fluoro-anilino)propanamide 166.1

Example 166 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 5.01-5.04 (m, 1H), 5.12 (s, 2H), 7.00-7.05 (m, 2H), 7.35-7.45 (m, 9H), 7.63 (s, 1H), 7.73-7.77 (m, 2H), 8.08-8.13 (m, 2H) 39% yield Chiral HPLC Example 166.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(benzyloxylbenzoyl)thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide (790 mg, 1.5 mmol, Example 166) on a chiral column gave 313 mg (39% yield) of 2-(N-[4-amino-5-(4-(benzylozybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 100 mL/min; temperature: 25° C.; UV: 325 nm Analytical chiral HPLC: Rt = 2.94 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 325 nm 166.2

Example 166 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 5.01-5.05 (m, 1H), 5.12 (s, 2H), 7.00-7.05 (m, 2H), 7.35-7.45 (m, 9H), 7.63 (s, 1H), 7.73-7.77 (m, 2H), 8.08-8.13 (m, 2H) 35% yield HPLC separation of rac-2-(N-[4-amino-5-[4-(benzyloxylbenzoyl)thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide (790 mg, 1.5 mmol, Example 166) on a chiral column gave 276 mg (35% yield) of 2-(N-[4-amino-5-(4-(benzylozybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 100 mL/min; temperature: 25° C.; UV: 325 nm Analytical chiral HPLC: Rt = 3.51 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 325 nm 167

Intermediate 109, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.60 Hz, 3H), 5.01 (br d, J = 7.35 Hz, 1H), 5.15 (s, 2H), 7.28 (s, 1H), 7.40 (m, 6H), 7.48 (m, 5H), 7.57 (m, 1H), 7.61 (s, 1H), 7.77 (ddd, J = 11.41, 7.48, 2.41 Hz, 1H), 7.95 (m, 2H) Biotage (method X) LC-MS (method 2) Rt = 1.22 min MS (ESIpos): m/z = 552.4 [M + H]⁺ 64% yield 167.1 (R)-benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-3,4-difluoro- and anilino)thiazole-5-carbonyl]phenyl]carbamate and (S)-benzyl N-[4-[4-amino-2- 167.2 (N-(2-amino-1-methyl-2-oxo-ethyl)-3,4-difluoro-anilino)thiazole-5- carbonyl]phenyl]carbamate 167.1

Example 167 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 3H), 5.01 (q, J = 7.35 Hz, 1H), 5.15 (s, 2H), 5.76 (s, 1H), 7.28 (s, 1H), 7.35-7.42 (m, 5H), 7.46-7.52 (m, 5H), 7.57 (m, 1H), 7.61 (s, 1H), 7.78 (m, 1H), 8.18 (m, 2H), 10.00 (s, 1H) 32% yield Chiral HPLC Example 167.1 HPLC separation of rac-benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl-3,4-difluoro-anilino)thiazole-5- carbonyl]phenyl]carbamate (385 mg, 0.7 mmol, Example 167) on a chiral column followed by trituration in MTBE gave 123 mg (32% yield) of benzyl (N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-3,4-difluoro-anilino)thiazole-5-carbamate, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 2.87 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 325 nm 167.2

Example 167 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 3H), 5.01 (q, J = 7.35 Hz, 1H), 5.15 (s, 2H), 5.76 (s, 1H), 7.28 (s, 1H), 7.40 (m, 5H), 7.48 (m, 5H), 7.57 (m, 1H), 7.61 (s, 1H), 7.78 (m, 1H), 8.18 (m, 2H), 10.00 (s, 1H) 34% yield Chiral HPLC Example 167.2 HPLC separation of rac-benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl-3,4-difluoro-anilino)thiazole-5- carbonyl]phenyl]carbamate (385 mg, 0.7 mmol, Example 167) on a chiral column followed by trituration in MTBE gave 129 mg (34% yield) of benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-3,4-difluoro-anilino)thiazole-5-carbonyl]phenyl]carbamate, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 3.90 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 90% A + 10% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 325 nm 168

Intermediate 112, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.60 Hz, 3H), 2.01 (s, 3H), 4.18 (dd, J = 5 32, 3.55 Hz, 2H), 4.30 (dd, J = 5.45, 3.42 Hz, 2H), 5.06 (m, 1H), 6.94 (m, 2H), 7.23 (s, 1H), 7.33 (t, J = 8.74 Hz, 2H), 7.47 (d, J = 8.87 Hz, 2H), 7.58 (s, 1H), 7.63 (m, 2H), 8.06 (m, 2H) RP-HPLC (method C basic) LC-MS (method 1) Rt = 1.04 min MS (ESIpos): m/z = 487.6 [M + H]⁺ 54% yield 169

Intermediate 132, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.05 (q, 0 = 7.35 Hz, 1H), 5.15 (s, 2H), 7.25 (s, 1H), 7.40 (m, 11H), 7.58 (s, 1H), 7.64 (m, 2H), 8.12 (m, 2H), 9.99 (m, 1H) Biotage (method X) LC-MS (method 1) Rt = 1.19 min MS (ESIpos): m/z = 534.4 [M + H]⁺ 65% yield 169.1 (R)-benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro- and anilino)thiazole-5-carbonyl]phenyl]carbamate and (S)-benzyl N-[4-[4-amino-2- 169.2 (N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5- carbonyl]phenyl]carbamate 169.1

Example 169 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.05 (q, J = 7.35 Hz, 1H), 5.15 (s, 2H), 7.25 (s, 1H), 7.40 (m, 11H), 7.58 (s, 1H), 7.64 (m, 2H), 8.12 (m, 2H), 9.99 (m, 1H) LC-MS (method 1) Rt = 1.19 min MS (ESIpos): m/z = 534.4 [M + H]⁺ 14% yield Chiral HPLC Example 169.1 HPLC separation of rac-benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl-4-fluoro-anilino)thiazole-5- carbonyl]phenyl]carbamate (422 mg, 0.76 mmol, Example 169) on a chiral column followed by trituration in MTBE gave 88 mg (14% yield) of benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]phenyl]carbamate, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 80 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 1.39 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 169.2

Example 169 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.05 (q, J = 7.35 Hz, 1H), 5.15 (s, 2H), 7.25 (s, 1H), 7.40 (m, 11H), 7.58 (s, 1H), 7.64 (m, 2H), 8.12 (m, 2H), 9.99 (m, 1H) LC-MS (method 1) Rt = 1.19 min MS (ESIpos): m/z = 534.4 [M + H]⁺ 16% yield Chiral HPLC Example 169.2 HPLC separation of rac-benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl-4-fluoro-anilino)thiazole-5- carbonyl]phenyl]carbamate (422 mg, 0.76 mmol, Example 169) on a chiral column followed by trituration in MTBE gave 106 mg (14% yield) of benzyl N-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]phenyl]carbamate, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Amylose SA 10μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 80 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 1.80 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 280 nm 170

Intermediate 110, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.05 (m, 1H), 5.11 (s, 2H), 7.00 (m, 2H), 7.25 (s, 1H), 7.37 (m, 7H), 7.48 (m, 2H), 7.57 (s, 1H), 7.64 (m, 2H), 8.11 (m, 2H) RP-HPLC (method B basic) LC-MS (method 2) Rt = 1.25 min MS (ESIpos): m/z = 491.3 [M + H]⁺ 88% yield 170.1 (R)-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2-yl]-4-fluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2- 170.2 yl]-4-fluoro-anilino)propanamide 170.1

Example 170 ¹HNMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.02-5.06 (m, 1H), 5.11 (s, 2H), 7.00 (m, 2H), 7.25 (s, 1H), 7.30-7.40 (m, 7H), 7.46-7.50 (m, 2H), 7.57 (s, 1H), 7.62-7.66 (m, 2H), 8.08-8.12 (m, 2H) LC-MS (method 2) Rt = 1.25 min MS (ESIpos): m/z = 491.3 [M + H]⁺ 35% yield Chiral HPLC Example 170.1 HPLC separation of rac-2-(N-[4-amino-5-(4-benzoyl))thiazole-2-yl]-4-fluoro-anilino)propanamide (150 mg, 0.3 mmol, Example 170) on a chiral column gave 53 mg (35% yield) of 2-(N-[4-benzyloxybenzoyl)thiazole-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec:Prep SFC100; Column: Chiralpak IB 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4% diethylamine; isocratic: 35% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 3.08 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IB 5μ, 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 35%B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 170.2

Example 170 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.04-5.07 (m, 1H), 5.11 (s, 2H), 6.97-7.02 (m, 2H), 7.25 (s, 1H), 7.30-7.40 (m, 7H), 7.46-7.50 (m, 2H), 7.57 (s, 1H), 7.62-7.66 (m, 2H), 8.09-8.11 (m, 2H) LC-MS (method 2) Rt = 1.25 min MS (ESIpos): m/z = 491.3 [M + H]⁺ 34% yield Chiral HPLC Example 170.2 HPLC separation of rac-2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazole-2-yl]-4-fluoro-anilino)propanamide (150 mg, 0.3 mmol, Example 170) on a chiral column gave 51 mg (34% yield) of 2-N-[4-amino-5-(4-benzyloxy)thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec:Prep SFC100; Column: Chiralpak IB 5μ 250 × 30 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4% diethylamine; isocratic: 35% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 4.42 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IB 5μ, 100 × 4.6 mm; eluent A: CO₂; eluent B: 2-propanol + 0.4 vol % diethylamine; isocratic: 35% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 171

Intermediate 111, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.07 (q, J = 7.35 Hz, 1H), 7.29 (m, 5H), 7.58 (m, 1H), 7.63 (m, 2H), 7.76 (m, 2H), 8.21 (m, 2H) Biotage (method X) LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 511.0 [M + H]⁺ 39% yield 172

Intermediate 113, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 3.87 (s, 3H), 5.03 (q, J = 7.35 Hz, 1H), 6.85 (dd, J = 8.62, 0.76 Hz, 1H), 7.31 (s, 1H), 7.52 (m, 1H), 7.64 (s, 1H), 7.76 (m, 2H), 7.84 (dd, J = 8.49, 2.41 Hz, 1H), 8.14 (m, 2H), 8.37 (m, 1H) Biotage (method Y) LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 450.3 [M + H]⁺ 38% yield 172.1 (R)-2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazoi-2-yl]-4-chloro-3- and fluoro-anilino)propanamide and (S)-2-(N-[4-amino-5-(6-methoxypyridine-3- 172.2 carbonyl)thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide 172.1

Example 172 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 3.87 (s, 3H), 5.03 (q, J = 7.35 Hz, 1H), 6.85 (dd, J = 8 62, 0.76 Hz, 1H), 7.31 (s, 1H), 7.52 (m, 1H), 7.64 (s, 1H), 7.76 (m, 2H), 7.84 (dd, J = 8.49, 2.41 Hz, 1H), 8.14 (m, 2H), 8.37 (m, 1H) LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 450.3 [M + H]⁺ 24% yield Chiral HPLC Example 172.1 HPLC separation of rac-2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazole-2-yl]-4-chloro-3-fluoro-anilino)propanamide (312 mg, 0.69 mmol, Example 172) on a chiral column gave 78 mg (24% yield) of 2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazol-2-yl]- 4-chloro-3-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec:Prep SFC100; Column: Chiralpak IG 5μ 250 × 30 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic; 30% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 2.61 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IG 5μ, 100 × 4.6 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%) B; isocratic: 30% ; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 172.2

Example 172 ¹HNMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3H), 3.87 (s, 3H), 5.03 (q, J = 7.35 Hz, 1H), 6.85 (dd, J = 8 62, 0.76 Hz, 1H), 7.31 (s, 1H), 7.52 (m, 1H), 7.64 (s, 1H), 7.76 (m, 2H), 7.84 (dd, J = 8.49, 2.41 Hz, 1H), 8.14 (m, 2H), 8.37 (m, 1H) LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 450.3 [M + H]⁺ 23% yield Chiral HPLC Example 172.2 HPLC separation of rac-2-(N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazole-2-yl]-4-chloro-3-fluoro-anilino)propanamide (312 mg, 0.69 mmol, Example 172) on a chiral column gave 73 mg (23% yield) of 2-N-[4-amino-5-(6-methoxypyridine-3-carbonyl)thiazol-2-yl]-4- chloro-3-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec:Prep SFC100; Column: Chiralpak IG 5μ 250 × 30 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic; 30% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 3.40 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IG 5μ, 100 × 4.6 mm; eluent A: CO₂; eluent B: methanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 173

Intermediate 114, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.07 (m, 1H), 6.94 (m, 2H), 7.06 (m, 2H), 7.20 (m, 1H), 7.25 (s, 1H), 7.33 (dd, J = 8.87 Hz, 2H), 7.41 (m, 2H), 7.53 (m, 2H), 7.58 (s, 1H), 7.64 (m, 2H), 8.14 (m, 2H) Biotage (method X) LC-MS (method 2) Rt = 1.26 min MS (ESIpos): m/z = 477.2 [M + H]⁺ 56% yield 174

Intermediate 115, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 3.73 (s, 3H), 5.06 (q, J = 7.35 Hz, 1H), 7.01 (d, J = 9.13 Hz, 2H), 7.16 (m, 2H), 7.21 (s, 1H), 7.27 (t, J = 72 Hz, 1H), 7.46 (m, 2H), 7.54 (m, 3H), 8.15 (m, 2H) Biotage (method X) LC-MS (method 2) Rt = 1.08 min MS (ESIpos): m/z = 463.2 [M + H]⁺ 77% yield 174.1 (R)-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-methoxy- and anilino)propanamide and (S)-2-(N-[4-amino-5-[4- 174.2 (difluoromethoxy)benzoyl]thiazol-2-yl]-4-methoxy-anilino)propanamide 174.1

Example 174 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 3.73 (s, 3H), 5.06 (q, 0 = 7.35 Hz, 1H), 7.01 (d, J = 9.13 Hz, 2H), 7.16 (m, 2H), 7.21 (s, 1H), 7.27 (t, J = 72 Hz, 1H), 7.46 (m, 2H), 7.54 (m, 3H), 8.15 (m, 2H) LC-MS (method 2) Rt = 1.08 min MS (ESIpos): m/z = 463.2 [M + H]⁺ 31% yield Chiral HPLC Example 174.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazole-2-yl]-4-methoxy-anilino)propanamide (316 mg, 0.66 mmol, Example 174 on a chiral column gave 121 mg (31% yield) of 2-N-[4-amino-5-[4-(difluoromethoxy)benzoyl)thiazol-2-yl]-4- methoxy-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Amylose SA 10μ 250 × 50 mm; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: methanol; isocratic; 50% A +50% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.28 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6 mm; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; 254 nm 174.2

Example 174 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 3.73 (s, 3H), 5.06 (q, 0 = 7.35 Hz, 1H), 7.01 (d, J = 9.13 Hz, 2H), 7.16 (m, 2H), 7.21 (s, 1H), 7.27 (t, J = 72 Hz, 1H), 7.46 (m, 2H), 7.54 (m, 3H), 8.15 (m, 2H) LC-MS (method 2) Rt = 1.08 min MS (ESIpos): m/z = 463.2 [M + H]⁺ 31% yield Chiral HPLC Example 174.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazole-2-yl]-4-methoxy-anilino)propanamide (316 mg, 0.66 mmol, Example 174 on a chiral column gave 121 mg (31% yield) of 2-N-[4-amino-5-[4-(difluoromethoxy)benzoyl)thiazol-2-yl]-4- methoxy-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Amylose SA 10μ 250 × 50 mm; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: methanol; isocratic; 50% A +50% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.62 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Amylose SA 3μ, 100 × 4.6 mm; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; 254 nm 175

Intermediate 116, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 5.07 (q, J = 7.35 Hz, 1H), 7.26 (br s, 1H), 7.33 (m, 3H), 7.59 (br s, 1H), 7.63 (m, 3H), 7.71 (m, 2H), 8.23 (m, 3H), 8.38 (m, 1H) Biotage (method X) LC-MS (method 2) Rt = 1.05 min MS (ESIpos): m/z = 430.1 [M + H]⁺ 66% yield 176

Intermediate 117, rac-2- bromo- propanamide ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.57 Hz, 3H), 2.29 (s, 3H), 3.80 (s, 3H), 5.11 (q, J = 7.35 Hz, 1H), 6.99 (d, J = 8.83 Hz, 2H), 7.20 (s, 1H), 7.36 (dd, J = 8.83 Hz, 2H), 7.58 (m, 2H), 7.61 (s, 1H), 7.68 (m, 2H) LC-MS (method 2) Rt = 1.16 min MS (ESIpos): m/z = 414.5 [M + H]⁺ 79% yield 176.1 (R)-2-(4-fluoro-N-[5-(4-methoxybenzoyl)-4-methyl-thiazol-2- and yl]anilino)propanamide and (S)-2-(4-fluoro-N-[5-(4-methoxybenzoy)-4- 176.2 methyl-thiazol-2-yl]anilino)propanamide 176.1

Example 176 ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.57 Hz, 3H), 2.29 (s, 3H), 3.80 (s, 3H), 5.11 (q, J = 7 35 Hz, 1H), 6.99 (d, J = 8.83 Hz, 2H), 7.20 (s, 1H), 7.36 (dd, J = 8.83 Hz, 2H), 7.58 (m, 2H), 7.61 (s, 1H), 7.68 (m, 2H) LC-MS (method 2) Rt = 1.16 min MS (ESIpos): m/z = 414.5 [M + H]⁺ 49% yield Chiral HPLC Example 176.1 HPLC separation of rac-2-(4-fluoro-N-[5-(4-methoxybenzoyl)-4-methyl-thiazole-2-yl]anilino)propanamide (80 mg, 0.19 mmol, Example 176 on a chiral column gave 39 mg (49% yield) of 2-(4-fluoro-N-[5-(4-methoxybenzoyl)-4-methyl-thiazole-2-yl]anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10μ 250 × 50 mm; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic; 80% A +20% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.20 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; 254 nm 176.2

Example 176 ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.57 Hz, 3H), 2.29 (s, 3H), 3.80 (s, 3H), 5.11 (q, J = 7 35 Hz, 1H), 6.99 (d, J = 8.83 Hz, 2H), 7.20 (s, 1H), 7.36 (dd, J = 8.83 Hz, 2H), 7.58 (m, 2H), 7.61 (s, 1H), 7.68 (m, 2H) LC-MS (method 2) Rt = 1.16 min MS (ESIpos): m/z = 414.5 [M + H]⁺ 49% yield Chiral HPLC Example 176.2 HPLC separation of rac-2-(4-fluoro-N-[5-(4-methoxybenzoyl)-4-methyl-thiazole-2-yl]anilino)propanamide (80 mg, 0.19 mmol, Example 176 on a chiral column gave 31 mg (39% yield) of 2-(4-fluoro-N-[5-(4-methoxybenzoyl)-4-methyl-thiazole-2-yl]anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10μ 250 × 50 mm; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic; 80% A +20% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 4.84 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; 254 nm 177

Intermediate 118, rac-2- bromo- propanamide ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 0.75-0.80 (m, 4H), 1.16 (d, J = 7.31 Hz, 3H), 1.77 (m, 1H), 5.05 (m, 1H), 7.24 (br s, 1H), 7.32 (dd, J = 8.74 Hz, 2H), 7.45 (d, J = 8.58 Hz, 2H), 7.57 (m, 3H), 7.64 (dd, J = 8.74, 4.93 Hz, 2H), 8.11 (m, 2H), 10.34 (s, 1H) RP-HPLC (method C basic) LC-MS (method 1) Rt = 0.99 min MS (ESIpos): m/z = 468.5 [M + H]⁺ 57% yield 178

Intermediate 119, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 3.16 (m, 4H), 3.70 (m, 4H), 5.06 (q, J = 7.35 Hz, 1H), 6.90 (d, J = 8.87 Hz, 2H), 7.24 (br s, 1H), 7.34 (dd, J = 8.74 Hz, 2H), 7.43 (m, J = 8.87 Hz, 2H), 7.57 (br s, 1H), 7.65 (m, 2H), 7.97 (m, 2H) LC-MS (method 2) Rt = 1.01 min MS (ESIpos): m/z = 470.3 [M + H]⁺ 48% yield 179

Intermediate 120, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.14 (d, J = 7.35 Hz, 3H), 5.04 (q, 0 = 7.35 Hz, 1H), 5.32 (m, 2H), 6.27 (dd, 0 = 2.03 Hz, 1H), 7.15 (d, 0 = 8.36 Hz, 2H), 7.23 (s, 1H), 7.31 (m, 2H), 7.43 (m, 2H), 7.45 (m, 1H), 7.60 (m, 3H), 7.82 (dd, 0 = 2.28, 0.76 Hz, 1H), 8.18 (m, 2H) RP-HPLC (method C basic) LC-MS (method 1) Rt = 1.0 min MS (ESIpos): m/z = 465.6 [M + H]⁺ 66% yield 180

Intermediate 121, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, 0 = 7.35 Hz, 3H), 2.92 (s, 6H), 5.05 (q, 0 = 7.35 Hz, 1H), 6.65 (m, 2H), 7.23 (s, 1H), 7.34 (dd, 0 = 8.87 Hz, 2H), 7.43 (m, 2H), 7.57 (br d, 0 = 0.76 Hz, 1H), 7.65 (m, 2H), 7.96 (m, 2H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.13 min MS (ESIpos): m/z = 428.5 [M + H]⁺ 63% yield 181

Intermediate 122, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (br d, 0 = 7.35 Hz, 3H), 1.93 (m, 4H), 3.23 (m, 4H), 5.06 (m, 1H), 6.47 (br d, 0 = 8.62 Hz, 2H), 7.24 (m, 1H), 7.34 (s, 2H), 7.42 (br d, 0 = 8.36 Hz, 2H), 7.57 (br s, 1H), 7.65 (m, 2H), 7.97 (m, 2H) LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 454.3 [M + H]⁺ 82% yield 182

Intermediate 123, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 5.03-5.06 (m, 1H), 5.08-5.12 (m, 2H), 7.09-7.14 (m, 4H), 7.22 (s, 1H), 7.28 (s, 1H), 7.35-7.45-7.62 (m, 10H), 8.10-8.15 (m, 2H) Biotage (method X) LC-MS (method 2) Rt = 1.29 min MS (ESIpos): m/z = 539.4 [M + H]⁺ 73% yield 182.1 (R)-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-4-benzyloxy- and anilino)propanamide and (S)-2-(N-[4-amino-5-[4- 182.2 (difluoromethoxy)benzoyl]thiazol-2-yl]-4-benzyloxy-anilino)propanamide 182.1

Example 182 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 5.05 (m, 1H), 5.11 (m, 2H), 7.13 (m, 4H), 7.22 (s, 1H), 7.28 (s, 1H), 7.35-7.55 (m, 10H), 8.13 (m, 2H) LC-MS (method 2) Rt = 1.29 min MS (ESIpos): m/z = 539.4 [M + H]⁺ 44% yield Chiral HPLC Example 182.1 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazole-2-yl]-4-benzyloxy-anilino)propanamide (813 mg, 1.51 mmol, Example 182 on a chiral column gave 360 mg (44% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazole-2-yl]-4- benzyloxy-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Cellulose SB 10μ 250 × 50 mm; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic; 60% A +40% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.76 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 182.2

Example 182 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 5.03-5.06 (m, 1H), 5.09-5.12 (m, 2H), 7.09-7.14 (m, 4H), 7.22 (s, 1H), 7.28 (s, 1H), 7.35-7.55 (m, 10H), 8.10-8.15 (m, 2H) LC-MS (method 2) Rt = 1.29 min MS (ESIpos): m/z = 539.4 [M + H]⁺ 45% yield Chiral HPLC Example 182.2 HPLC separation of rac-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazole-2-yl]-4-benzyloxy-anilino)propanamide (813 mg, 1.51 mmol, Example 182 on a chiral column gave 365 mg (45% yield) of 2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazole-2-yl]-4- benzyloxy-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-3; Column: YMC Cellulose SB 10μ 250 × 50 mm; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic; 60% A +40% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.49 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 183

Intermediate 124, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 5.03-5.06 (m, 1H), 7.20-7.28 (m, 4H), 7.33 (d, J = 8.87 Hz, 3H), 7.42 (d, J = 8.11 Hz, 1H), 7.59-7.63 (m, 3H), 8.17-8.21 (m, 2H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.13 min MS (ESIpos): m/z = 451.5 [M + H]⁺ 41% yield 184

Intermediate 125, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3H), 4.97-5.09 (m, 1H), 7.30 (s, 1H), 7.40-7.48 (m, 1H), 7.48-7.53 (m, 1H), 7.55-7.61 (m, 1H), 7.63 (s, 1H), 7.74-7.81 (m, 1H), 7.85-7.90 (m, 1H), 8.07-8.41 (m, 2H), 8.61 (dd, J = 4.82, 1.52 Hz, 1H), 8.69 (dd, J = 2.28, 0.76 Hz, 1H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 0.92 min MS (ESIpos): m/z = 404.3 [M + H]⁺ 54% yield 184.1 (R)-2-(N-[4-amino-5-(pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(pyridine-3-carbonyl)thiazol-2- 184.2 yl]-3,4-difluoro-anilino)propanamide 184.1

Example 184 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3H), 4.97-5.09 (m, 1H), 7.30 (s, 1H), 7.40-7.48 (m, 1H), 7.48-7.53 (m, 1H), 7.55-7.61 (m, 1H), 7.63 (s, 1H), 7.74-7.81 (m, 1H), 7.85-7.90 (m, 1H), 8.07-8.41 (m, 2H), 8.61 (dd, J = 4.82, 1.52 Hz, 1H), 8.69 (dd, J = 2.28, 0.76 Hz, 1H) LC-MS (method 2) Rt = 0.92 min MS (ESIpos): m/z = 404.3 [M + H]⁺ 39% yield Chiral HPLC Example 184.1 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide (115 mg, 0.29 mmol, Example 184) on a chiral column gave 45 mg (39% yield) of 2-(N-[4-amino-5-(pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10μ 250 × 50 mm; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic; 50% A +50% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 2.11 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; 254 nm 184.2

Example 184 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3H), 4.97-5.09 (m, 1H), 7.30 (s, 1H), 7.40-7.48 (m, 1H), 7.48-7.53 (m, 1H), 7.55-7.61 (m, 1H), 7.63 (s, 1H), 7.74-7.81 (m, 1H), 7.85-7.90 (m, 1H), 8.07-8.41 (m, 2H), 8.61 (dd, J = 4.82, 1.52 Hz, 1H), 8.69 (dd, J = 2.28, 0.76 Hz, 1H) LC-MS (method 2) Rt = 0.92 min MS (ESIpos): m/z = 404.3 [M + H]⁺ 43% yield Chiral HPLC Example 184.2 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro-anilino)propanamide (115 mg, 0.29 mmol, Example 184) on a chiral column gave 50 mg (43% yield) of 2-(N-[4-amino-5-(pyridine-3-carbonyl)thiazol-2-yl]-3,4-difluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10μ 250 × 50 mm; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic; 50% A +50% B; flow: 100 mL/min; temperature: 25° C.; UV: 280 nm Analytical chiral HPLC: Rt = 2.83 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; 254 nm 185

Intermediate 126, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 2.03 (s, 3H), 5.05 (q, J = 7.35 Hz, 1H), 7.24 (s, 1H), 7.32 (dd, J = 8.87 Hz, 2H), 7.44 (d, J = 8.87 Hz, 2H), 7.55 (d, J = 8.62 Hz, 2H), 7.58 (br s, 1H), 7.61-7.66 (m, 2H), 7.81-8.39 (m, 2H), 10.11 (br s, 1H) RP-HPLC (method C basic) LC-MS (method 1) Rt = 0.87 min MS (ESIpos): m/z = 442.4 [M + H]⁺ 54% yield 186

Intermediate 127, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.60 Hz, 3H), 5.00-5.13 (m, 1H), 7.27 (s, 1H), 7.34 (dd, J = 8.87 Hz, 2H), 7.41 (dd, J = 5.07, 1.27 Hz, 1H), 7.49 (s, 1H), 7.59 (s, 1H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 8.20-8.42 (m, 2H), 8.45 (d, J = 5.07 Hz, 1H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.03 min MS (ESIpos): m/z = 420.3 [M + H]⁺ 25% yield 187

Intermediate 128, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.60 Hz, 3H), 2.45 (s, 3H), 5.06 (br s, 1H), 7.10-7.23 (m, 1H), 7.23-7.28 (m, 2H), 7.33 (dd, J = 8.74 Hz, 2H), 7.59 (br s, 1H), 7.63 (dd, J = 8.87, 5.07 Hz, 2H), 8.05-8.42 (m, 2H), 8.46 (d, J = 5.07 Hz, 1H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 0.90 min MS (ESIpos): m/z = 400.5 [M + H]⁺ 66% yield 187.1 (R)-2-(N-[4-amino-5-(2-methylpyridine-4-carbonyl)thiazol-2-yl]-4-fluoro- and anilino)propanamide and (S)-2-(N-[4-amino-5-(2-methylpyridine-4- 187.2 carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide 187.1

Example 187 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.60 Hz, 3H), 2.45 (s, 3H), 5.06 (br s, 1H), 7.10-7.23 (m, 1H), 7.23-7.28 (m, 2H), 7.33 (dd, J = 8.74 Hz, 2H), 7.59 (br s, 1H), 7.63 (dd, J = 8.87, 5.07 Hz, 2H), 8.05-8.42 (m, 2H), 8.46 (d, J = 5.07 Hz, 1H) 32% yield Chiral HPLC Example 187.1 HPLC separation of rac-2-(N-[4-amino-5-(2-methylpyridine-4-carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (333 mg, 0.83 mmol, Example 187) on a chiral column gave 105 mg (32% yield) of 2-(N-[4-amino-5-(2-methylpyridine-4-carbonyl)thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC 100; Column: Chiralpak IG 5μ 250 × 30 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic; 15% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 2.81 min Instrument: Agilent 1260, Aurora SFC-Modul; Column: Chiralpak IG 5μ, 100 × 4.6; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 25% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 187.2

Example 187 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.60 Hz, 3H), 2.45 (s, 3H), 5.06 (br s, 1H), 7.10-7.23 (m, 1H), 7.23-7.28 (m, 2H), 7.33 (dd, J = 8.74 Hz, 2H), 7.59 (br s, 1H), 7.63 (dd, J = 8.87, 5.07 Hz, 2H), 8.05-8.42 (m, 2H), 8.46 (d, J = 5.07 Hz, 1H) 23% yield Chiral HPLC Example 187.2 HPLC separation of rac-2-(N-[4-amino-5-(2-methylpyridine-4-carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (333 mg, 0.83 mmol, Example 187) on a chiral column gave 78 mg (23% yield) of 2-(N-[4-amino-5-(2-methylpyridine-4-carbonyl)thiazol-2-yl]-4-fluoro- anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC 100; Column: Chiralpak IG 5μ 250 × 30 mm; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic; 15% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 3.56 min Instrument: Agilent 1260, Aurora SFC-Modul; Column: Chiralpak IG 5μ, 100 × 4.6; eluent A: CO₂; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 25% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 188

Intermediate 129, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3H), 4.99-5.13 (m, 1H), 6.97 (t, J = 52 Hz, 1H), 7.27 (s, 1H), 7.33 (t, J = 8.87 Hz, 2H), 7.56-7.61 (m, 2H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 7.67 (s, 1H), 8.20-8.57 (m, 2H), 8.73 (d, J = 4.56 Hz, 1H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.00 min MS (ESIpos): m/z = 436.3 [M + H]⁺ 13% yield 189

Intermediate 130, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.22 (d, J = 7.35 Hz, 3H), 5.05 (q, J = 7.35 Hz, 1H), 5.74 (s, 1H), 7.32 (s, 1H), 7.37-7.44 (m, 2H), 7.48-7.55 (m, 2H), 7.56-7.65 (m, 3H), 8.06-8.42 (m, 2H), 8.68-8.73 (m, 2H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 0.80 min MS (ESIpos): m/z = 368.2 [M + H]⁺ 17% yield 190

Intermediate 131, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.15 (d, J = 7.35 Hz, 3H), 3.83 (s, 3H), 5.00-5.11 (m, 1H), 6.76 (s, 1H), 6.99 (dd, J = 5.20, 1.39 Hz, 1H), 7.26 (s, 1H), 7.30-7.37 (m, 2H), 7.59 (s, 1H), 7.63 (dd, J = 8.87, 5.07 Hz, 2H), 8.17-8.20 (m, 1H), 8.21-8.48 (m, 2H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 0.98 min MS (ESIpos): m/z = 416.2 [M + H]⁺ 29% yield 191

Intermediate 133; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.12 (br s, 2H), 7.57 (s, 1H), 7.46-7.53 (m, 3H), 7.34-7.44 (m, 4H), 7.21-7.28 (m, 2H), 4.98-5.10 (m, 1H), 3.87 (s, 3H), 1.16 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.06 min MS (ESIpos): m/z = 415.3 [M + H]⁺ 78% yield 192

Intermediate 134; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1H NMR (400 MHz, DMSO-d6, 22° C.): Shift = 7.95-8.40 (m, 3H), 7.86 (dd, J = 10.4, 1.8 Hz, 1H), 7.64-7.72 (m, 2H), 7.50-7.55 (m, 2H), 7.38-7.48 (m, 3H), 7.36 (s, 1H), 5.01 (q, J = 7.4 Hz, 1H), 1.22 (d, J = 7.4 Hz, 3H) LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 410.3 [M + H]⁺ 18% yield 193

Intermediate 135; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.17 (br s, 2H), 7.66-7.72 (m, 2H), 7.59 (s, 1H), 7.51-7.57 (m, 2H), 7.46-7.51 (m, 2H), 7.36-7.44 (m, 3H), 7.26 (s, 1H), 5.05 (q, J = 7.3 Hz, 1H), 1.16 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 1.19 min MS (ESIpos): m/z = 445.3 [M + H]⁺ 47% yield 194

Intermediate 136; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.13 (br s, 2H), 7.90 (d, J = 2.5 Hz, 1H), 7.60-7.68 (m, 2H), 7.45-7.52 (m, 3H), 7.39-7.44 (m, 3H), 7.39 (t J = 73.0 Hz, 1H), 7.29 (s, 1H), 5.02 (q, J = 7.1 Hz, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.18 min MS (ESIpos): m/z = 467.4 [M + H]⁺ 68% yield 195

Intermediate 137; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.11 (br s, 2H), 7.52 (s, 1H), 7.41-7.49 (m, 4H), 7.34-7.41 (m, 3H), 7.20 (s, 1H), 6.98 (d, J = 9.1 Hz, 2H), 5.00-5.12 (m, 1H), 4.03 (q, J = 6.8 Hz, 2H), 1.32 (t, J = 7.0 Hz, 3H), 1.14 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 411.5 [M + H]⁺ 61% yield 196

Intermediate 138; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.08 (br s, 2H), 7.54 (s, 1H), 7.46-7.51 (m, 2H), 7.36-7.44 (m, 3H), 7.22 (s, 1H), 7.14 (d, J = 1.8 Hz, 1H), 7.01-7.07 (m, 1H), 6.96-7.00 (m, 1H), 6.10 (s, 2H), 4.97-5.09 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.03 min MS (ESIpos): m/z = 411.4 [M + H]⁺ 67% yield 197

Intermediate 139; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.13 (br s, 2H), 7.71 (d, J = 2.0 Hz, 1H), 7.61 (s, 1H), 7.45-7.56 (m, 4H), 7.35-7.44 (m, 3H), 7.28 (s, 1H), 5.03 (br d, J = 6.8 Hz, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.2 min MS (ESIpos): m/z = 447.3 [M + H]⁺ 51% yield 198

Intermediate 140; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.14 (br s, 2H), 7.69-7.76 (m, 1H), 7.62 (s, 1H), 7.46-7.52 (m, 4H), 7.36-7.46 (m, 3H), 7.34 (t, J = 73.0 Hz, 1H), 7.29 (s, 1H), 5.03 (q, J = 7.1 Hz, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.12 min MS (ESIpos): m/z = 451.3 [M + H]⁺ 49% yield 199

Intermediate 141; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.12 (br s, 2H), 7.53 (s, 1H), 7.44-7.51 (m, 6H), 7.32-7.43 (m, 6H), 7.21 (s, 1H), 7.09 (d, J = 9.1 Hz, 2H), 4.99-5.15 (m, 3H), 1.15 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.28 min MS (ESIpos): m/z = 473.4 [M + H]⁺ 57% yield 200

Intermediate 142; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.93-8.45 (m, 2H), 7.91 (d, J = 2.3 Hz, 1H), 7.63-7.67 (m, 1H), 7.62 (s, 1H), 7.47-7.53 (m, 3H), 7.40 (t, J = 73.0 Hz, 1H), 7.29 (s, 1H), 6.92-6.96 (m, 2H), 4.98-5.07 (m, 1H), 3.76 (s, 3H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.17 min MS (ESIpos): m/z = 497.3 [M + H]⁺ 63% yield 201

Intermediate 143; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.93-8.54 (m, 2H), 7.91 (d, J = 2.5 Hz, 1H), 7.61-7.68 (m, 2H), 7.55-7.61 (m, 2H), 7.08-7.53 (m, 6H), 4.98-5.08 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 533.3 [M + H]⁺ 58% yield 202

Intermediate 144; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.92-8.51 (m, 2H), 7.90 (d, J = 2.5 Hz, 1H), 7.60-7.68 (m, 2H), 7.50-7.55 (m, 2H), 7.45-7.50 (m, 3H), 7.39 (t, J = 73.0 Hz, 1H), 7.30 (s, 1H), 4.98-5.08 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.28 min MS (ESIpos): m/z = 501.3 [M + H]⁺ 56% yield 203

Intermediate 145; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.61-8.66 (m, 2H), 8.06-8.54 (m, 2H), 7.90 (d, J = 2.5 Hz, 1H), 7.61-7.67 (m, 2H), 7.48 (d, J = 8.6 Hz, 1H), 7.40-7.45 (m, 2H), 7.39 (t, J = 72.7 Hz, 1H), 7.31 (s, 1H), 4.98-5.07 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 0.98 min MS (ESIpos): m/z = 468.3 [M + H]⁺ 47% yield 204

Intermediate 146; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.41 (d, J = 1.8 Hz, 1H), 8.05-8.38 (m, 2H), 8.02 (dd, J = 8.6, 2.5 Hz, 1H), 7.91 (d, J = 2.5 Hz, 1H), 7.72 (t, J = 72.5 Hz, 1H), 7.61-7.68 (m, 2H), 7.47-7.52 (m, 1H), 7.40 (t, J = 72.8 Hz, 1H), 7.31 (s, 1H), 7.10-7.15 (m, 1H), 4.98-5.08 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 534.3 [M + H]⁺ 42% yield 205

Intermediate 147; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.77-8.42 (m, 2H), 7.74 (dd, J = 11.4, 1.3 Hz, 1H), 7.62 (s, 1H), 7.47-7.53 (m, 4H), 7.35 (t, J = 73.0 Hz, 1H), 6.91-6.97 (m, 2H), 4.99-5.08 (m, 1H), 3.76 (s, 3H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.13 min MS (ESIpos): m/z = 481.3 [M + H]⁺ 57% yield 206

Intermediate 148; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.80-8.55 (m, 2H), 7.73 (dd, J = 11.3, 1.4 Hz, 1H), 7.62 (s, 1H), 7.46-7.55 (m, 6H), 7.35 (t, J = 72.8 Hz, 1H), 7.30 (s, 1H), 4.99-5.08 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.24 min MS (ESIpos): m/z = 485.3 [M + H]⁺ 53% yield 207

Intermediate 149; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.78-8.47 (m, 2H), 7.73 (dd, J = 11.2, 1.3 Hz, 1H), 7.62 (s, 1H), 7.55-7.60 (m, 2H), 7.34-7.54 (m, 3H), 7.09-7.32 (m, 4H), 4.98-5.08 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.18 min MS (ESIpos): m/z = 517.3 [M + H]⁺ 50% yield 208

Intermediate 150; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.61-8.65 (m, 2H), 8.30 (br s, 2H), 7.73 (dd, J = 11.0, 1.4 Hz, 1H), 7.63 (s, 1H), 7.48-7.52 (m, 2H), 7.39-7.45 (m, 2H), 7.35 (t, J = 73.0 Hz, 1H), 7.31 (s, 1H), 4.98-5.08 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 0.94 min MS (ESIpos): m/z = 452.3 [M + H]⁺ 48% yield 209

Intermediate 151; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.41 (d, J = 2.0 Hz, 1H), 8.02 (dd, J = 8.5, 2.4 Hz, 3H), 7.74 (dd, J = 11.4, 1.3 Hz, 1H), 7.72 (t, J = 72.5 Hz, 1H), 7.63 (s, 1H), 7.49-7.53 (m, 2H), 7.36 (t, J = 73.0 Hz, 1H), 7.31 (s, 1H), 7.10-7.15 (m, 1H), 4.99-5.09 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.18 min MS (ESIpos): m/z = 518.3 [M + H]⁺ 51% yield 210

Intermediate 152; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.41 (d, J = 2.0 Hz, 1H), 8.02 (dd, J = 8.5, 2.4 Hz, 3H), 7.74 (dd, J = 11.4, 1.3 Hz, 1H), 7.72 (t, J = 72.5 Hz, 1H), 7.63 (s, 1H), 7.49-7.53 (m, 2H), 7.36 (t, J = 73.0 Hz, 1H), 7.31 (s, 1H), 7.10-7.15 (m, 1H), 4.99-5.09 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.18 min MS (ESIpos): m/z = 518.3 [M + H]⁺ 51% yield 211

Intermediate 153; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.01 (dd, J = 1.9, 0.9 Hz, 3H), 7.74 (d, J = 1.8 Hz, 2H), 7.66 (s, 1H), 7.49-7.55 (m, 2H), 7.37-7.47 (m, 3H), 7.32 (s, 1H), 4.95-5.05 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.29 min MS (ESIpos): m/z = 485.3 [M + H]⁺ 48% yield 212

Intermediate 154; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.80-8.36 (m, 2H), 7.72 (d, J = 1.8 Hz, 1H), 7.61 (s, 1H), 7.46-7.57 (m, 4H), 7.28 (s, 1H), 6.90-6.97 (m, 2H), 5.03 (br d, J = 7.6 Hz, 1H), 3.76 (s, 3H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.19 min MS (ESIpos): m/z = 476.3 [M + H]⁺ 67% yield 213

Intermediate 155; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.19 (br s, 2H), 7.71 (d, J = 2.0 Hz, 1H), 7.61 (s, 1H), 7.44-7.56 (m, 6H), 7.29 (s, 1H), 4.98-5.09 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.31 min MS (ESIpos): m/z = 481.2 [M + H]⁺ 59% yield 214

Intermediate 156; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.80-8.54 (m, 2H), 7.72 (d, J = 1.8 Hz, 1H), 7.61 (s, 1H), 7.53-7.60 (m, 3H), 7.47-7.52 (m, 1H), 7.30 (br s, 1H), 7.28 (t, J = 73.8 Hz, 1H), 7.18 (d, J = 8.6 Hz, 2H), 5.04 (br d, J = 6.3 Hz, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.23 min MS (ESIpos): m/z = 513.3 [M + H]⁺ 37% yield 215

Intermediate 157; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.60-8.65 (m, 2H), 8.07-8.53 (m, 2H), 7.71 (d, J = 2.0 Hz, 1H), 7.63 (s, 1H), 7.52-7.58 (m, 1H), 7.46-7.51 (m, 1H), 7.39-7.45 (m, 2H), 7.30 (s, 1H), 4.95-5.13 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 0.99 min MS (ESIpos): m/z = 448.3 [M + H]⁺ 64% yield 216

Intermediate 158; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.41 (d, J = 2.3 Hz, 3H), 8.02 (dd, J = 8.5, 2.4 Hz, 1H), 7.72 (d, J = 2.0 Hz, 1H), 7.71 (t, J = 72.5 Hz, 1H), 7.62 (s, 1H), 7.54-7.58 (m, 1H), 7.51 (d, J = 1.8 Hz, 1H), 7.30 (s, 1H), 7.11 (d, J = 8.4 Hz, 1H), 4.96-5.12 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.23 min MS (ESIpos): m/z = 514.3 [M + H]⁺ 59% yield 217

Intermediate 159; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.88-8.42 (m, 2H), 7.85 (dd, J = 11.2, 2.5 Hz, 1H), 7.75 (td, J = 8.7, 1.0 Hz, 1H), 7.64 (s, 1H), 7.58-7.63 (m, 1H), 7.48-7.55 (m, 2H), 7.32 (s, 1H), 6.91-6.97 (m, 2H), 5.01 (q J = 7.4 Hz, 1H), 3.77 (s, 3H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.23 min MS (ESIpos): m/z = 499.3 [M + H]⁺ 55% yield 218

Intermediate 160; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.90-8.49 (m, 2H), 7.85 (dd, J = 11.2, 2.3 Hz, 1H), 7.75 (td, J = 8.7, 1.0 Hz, 1H), 7.65 (s, 1H), 7.58-7.62 (m, 1H), 7.51-7.56 (m, 2H), 7.45-7.50 (m, 2H), 7.32 (s, 1H), 4.97-5.06 (m, 1H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.34 min MS (ESIpos): m/z = 503.3 [M + H]⁺ 46% yield 219

Intermediate 161; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.89-8.50 (m, 2H), 7.85 (dd, J = 11.2, 2.3 Hz, 1H), 7.70-7.80 (m, 1H), 7.65 (s, 1H), 7.57-7.63 (m, 3H), 7.32 (s, 1H), 7.29 (t, J = 73.8 Hz, 1H), 7.19 (d, J = 8.6 Hz, 2H), 5.02 (q, J = 7.4 Hz, 1H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.26 min MS (ESIpos): m/z = 525.3 [M + H]⁺ 40% yield 220

Intermediate 162; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.61-8.66 (m, 2H), 8.05-8.54 (m, 2H), 7.85 (dd, J = 11.2, 2.3 Hz, 1H), 7.71-7.78 (m, 1H), 7.66 (s, 1H), 7.60 (dd, J = 8.7, 1.4 Hz, 1H), 7.41-7.46 (m, 2H), 7.33 (s, 1H), 4.97-5.06 (m, 1H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 470.3 [M + H]⁺ 40% yield 221

Intermediate 163; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.42 (d, J = 2.0 Hz, 1H), 8.23 (br s, 2H), 8.03 (dd, J = 8.5, 2.4 Hz, 1H), 7.86 (dd, J = 11.2, 2.3 Hz, 1H), 7.73-7.79 (m, 1H), 7.72 (t, J = 72.5 Hz, 1H), 7.66 (s, 1H), 7.58-7.64 (m, 1H), 7.34 (s, 1H), 7.13 (dd, J = 8.6, 0.8 Hz, 1H), 4.97-5.06 (m, 1H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.29 min MS (ESIpos): m/z = 536.3 [M + H]⁺ 51% yield 222

Intermediate 164; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.78-8.36 (m, 2H), 7.74 (s, 2H), 7.66 (s, 1H), 7.49-7.55 (m, 2H), 7.32 (s, 1H), 6.91-6.98 (m, 2H), 5.00 (q, J = 7.0 Hz, 1H), 3.77 (s, 3H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.29 min MS (ESIpos): m/z = 515.3 [M + H]⁺ 46% yield 223

Intermediate 165; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.03-8.45 (m, 2H), 8.01 (t, J = 1.4 Hz, 1H), 7.74 (s, 2H), 7.66 (s, 1H), 7.51-7.57 (m, 2H), 7.45-7.50 (m, 2H), 7.33 (s, 1H), 4.96-5.05 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.4 min MS (ESIpos): m/z = 519.2 [M + H]⁺ 40% yield 224

Intermediate 166; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.04-8.37 (m, 2H), 8.01 (t, J = 1.3 Hz, 1H), 7.74 (s, 2H), 7.66 (s, 1H), 7.57-7.62 (m, 2H), 7.33 (s, 1H), 7.29 (t J = 73.8 Hz, 1H), 7.19 (d, J = 8.6 Hz, 2H), 5.00 (q, J = 7.0 Hz, 1H), 1.19 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 1.32 min MS (ESIpos): m/z = 551,3 [M + H]⁺ 42% yield 225

Intermediate 167; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.62-8.66 (m, 2H), 8.03-8.60 (m, 2H), 8.01 (t, J = 1.4 Hz, 1H), 7.74 (s, 2H), 7.67 (s, 1H), 7.41-7.46 (m, 2H), 7.34 (s, 1H), 4.95-5.04 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.09 min MS (ESIpos): m/z = 486.2 [M + H]⁺ 42% yield 226

Intermediate 168; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.42 (d, J = 1.8 Hz, 1H), 8.07-8.40 (m, 2H), 8.01-8.06 (m, 2H), 7.75 (s, 2H), 7.72 (t, J = 72.2 Hz, 1H), 7.67 (s, 1H), 7.34 (s, 1H), 7.13 (d, J = 9.1 Hz, 1H), 4.96-5.05 (m, 1H), 1.20 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 1.33 min MS (ESIpos): m/z = 552.2 [M + H]⁺ 34% yield 227

Intermediate 169; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.32 (d, J = 2.5 Hz, 1H), 7.93 (dd, J = 8.9, 2.8 Hz, 3H), 7.61 (s, 1H), 7.47-7.52 (m, 2H), 7.35-7.44 (m, 3H), 7.26 (s, 1H), 6.93 (d, J = 8.9 Hz, 1H), 4.98-5.16 (m, 1H), 3.87 (s, 3H), 1.17 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.01 min MS (ESIpos): m/z = 398.3 [M + H]⁺ 75% yield 228

Intermediate 170; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.56 (d, J = 2.5 Hz, 1H), 8.30 (dd, J = 8.7, 2.7 Hz, 1H), 8.15 (br s, 2H), 7.68 (s, 1H), 7.52 (dd, J = 7.6, 1.5 Hz, 2H), 7.37-7.48 (m, 4H), 7.33 (s, 1H), 4.97-5.07 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.16 min MS (ESIpos): m/z = 452.3 [M + H]⁺ 50% yield 229

Intermediate 171; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.46 (d, J = 2.3 Hz, 1H), 8.18 (dd, J = 8.7, 2.7 Hz, 3H), 7.74 (t, J = 72.2 Hz, 1H), 7.66 (s, 1H), 7.47-7.53 (m, 2H), 7.36-7.46 (m, 3H), 7.31 (s, 1H), 7.23 (d, J = 8.6 Hz, 1H), 5.04 (br d, J = 5.6 Hz, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.1 min MS (ESIpos): m/z = 434.3 [M + H]⁺ 68% yield 230

Intermediate 172; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.95 (s, 1H), 8.35-8.40 (m, 1H), 8.09 (br d, J = 8.4 Hz, 3H), 7.72 (s, 1H), 7.50-7.56 (m, 2H), 7.34-7.47 (m, 4H), 4.97-5.04 (m, 1H), 1.21 (br d, J = 7.1 Hz, 3H). LC-MS (method 2) Rt = 1.1 min MS (ESIpos): m/z = 436.3 [M + H]⁺ 20% yield 231

Intermediate 173; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.86 (d, J = 2.3 Hz, 1H), 7.89-8.50 (m, 3H), 7.86 (d, J = 8.4 Hz, 1H), 7.70 (s, 1H), 7.49-7.54 (m, 2H), 7.37-7.46 (m, 3H), 7.34 (s, 1H), 7.03 (t, J = 54.7 Hz, 1H), 5.03 (q, J = 7.4 Hz, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.0 min MS (ESIpos): m/z = 418.3 [M + H]⁺ 39% yield 232

Intermediate 174; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.61 (d, J = 2.8 Hz, 1H), 8.14 (dd, J = 8.4, 2.8 Hz, 3H), 7.69 (d, J = 8.6 Hz, 2H), 7.48-7.55 (m, 2H), 7.37-7.46 (m, 3H), 7.33 (br s, 1H), 4.96-5.07 (m, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.03 min MS (ESIpos): m/z = 402.2 [M + H]⁺ 49% yield 233

Intermediate 175; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.46 (d, J = 2.3 Hz, 1H), 7.72-8.42 (m, 3H), 7.67 (s, 1H), 7.48-7.54 (m, 2H), 7.33-7.47 (m, 4H), 7.32 (s, 1H), 4.97-5.10 (m, 1H), 1.19 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 0.97 min MS (ESIpos): m/z = 386.3 [M + H]⁺ 65% yield 234

Intermediate 176; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.58 (d, J = 2.3 Hz, 1H), 7.90 (dd, J = 8.1,2.5 Hz, 3H), 7.62 (s, 1H), 7.46-7.52 (m, 2H), 7.35-7.44 (m, 4H), 7.27 (s, 1H), 5.01-5.12 (m, 1H), 1.16 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 0.91 min MS (ESIpos): m/z = 382.3 [M + H]⁺ 69% yield 235

Intermediate 177; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.78-8.47 (m, 3H), 7.73 (td, J = 4.3, 2.5 Hz, 1H), 7.62-7.70 (m, 2H), 7.47-7.54 (m, 2H), 7.35-7.46 (m, 3H), 7.30 (s, 1H), 5.02 (br d, J = 6.8 Hz, 1H), 1.17 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.26 min MS (ESIpos): m/z = 469.3 [M + H]⁺ 52% yield 236

Intermediate 178; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.97 (dd, J = 2.0, 1.3 Hz, 3H), 7.85 (d, J = 8.6 Hz, 1H), 7.65-7.73 (m, 2H), 7.48-7.55 (m, 2H), 7.36-7.47 (m, 3H), 7.32 (s, 1H), 5.03 (q, J = 7.4 Hz, 1H), 1.18 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 1.33 min MS (ESIpos): m/z = 485.2 [M + H]⁺ 44% yield 237

Intermediate 179; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.77-8.54 (m, 2H), 7.72 (d, J = 7.6 Hz, 1H), 7.62 (s, 1H), 7.48-7.58 (m, 4H), 7.36-7.44 (m, 3H), 7.27 (s, 1H), 7.26 (t, J = 72.8 Hz, 1H), 4.97-5.08 (m, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 451.3 [M + H]⁺ 66% yield 238

Intermediate 180; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.78-8.54 (m, 2H), 7.69-7.77 (m, 2H), 7.63 (s, 1H), 7.48-7.55 (m, 3H), 7.36-7.45 (m, 3H), 7.09-7.33 (m, 2H), 5.02 (q, J = 7.4 Hz, 1H), 1.19 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 1.22 min MS (ESIpos): m/z = 467.3 [M + H]⁺ 51% yield 239

Intermediate 181; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.03-8.39 (m, 2H), 7.93-8.03 (m, 1H), 7.61-7.72 (m, 2H), 7.52 (br d, J = 4.3 Hz, 2H), 7.42 (br d, J = 7.1 Hz, 4H), 7.34 (br s, 1H), 4.65-5.38 (m, 1H), 1.09-1.27 (m, 3H). LC-MS (method 2) Rt = 1.19 min MS (ESIpos): m/z = 469.2 [M + H]⁺ 50% yield 240

Intermediate 182; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.88-8.43 (m, 3H), 7.64-7.87 (m, 2H), 7.60 (br d, J = 8.4 Hz, 1H), 7.27-7.56 (m, 6H), 4.93-5.30 (m, 1H), 1.09 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.24 min MS (ESIpos): m/z = 485.1 [M + H]⁺ 41% yield 241

Intermediate 183; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.94-8.49 (m, 2H), 7.88 (br s, 1H), 7.63 (br s, 1H), 7.50 (br s, 2H), 7.39-7.45 (m, 3H), 7.38 (t, J = 73.3 Hz, 1H), 7.35 (br d, J = 2.0 Hz, 1H), 7.31 (br s, 1H), 7.18 (br d, J = 8.9 Hz, 1H), 4.82-5.40 (m, 1H), 1.15 (br d, J = 1.3 Hz, 3H). LC-MS (method 2) Rt = 1.09 min MS (ESIpos): m/z = 451.2 [M + H]⁺ 38% yield 242

Intermediate 184; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.01 (d, J = 8.9 Hz, 3H), 7.60-7.72 (m, 1H), 7.20-7.59 (m, 9H), 4.97-5.21 (m, 1H), 1.09 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.13 min MS (ESIpos): m/z = 467.1 [M + H]⁺ 44% yield 243

Intermediate 185; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.77-8.41 (m, 2H), 7.67-7.76 (m, 4H), 7.60 (s, 1H), 7.45-7.52 (m, 2H), 7.27 (s, 1H), 7.10 (t, J = 55.8 Hz, 1H), 6.90-6.95 (m, 2H), 5.08 (q, J = 7.3 Hz, 1H), 3.75 (s, 3H), 1.17 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.05 min MS (ESIpos): m/z = 447.2 [M + H]⁺ 54% yield 244

Intermediate 186; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.81-8.54 (m, 2H), 7.67-7.75 (m, 4H), 7.60 (s, 1H), 7.48-7.53 (m, 2H), 7.43-7.48 (m, 2H), 7.28 (s, 1H), 7.09 (t, J = 55.5 Hz, 1H), 5.08 (q, J = 7.3 Hz, 1H), 1.17 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 451.1 [M + H]⁺ 48% yield 245

Intermediate 187; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 7.76-8.57 (m, 2H), 7.67-7.76 (m, 4H), 7.61 (s, 1H), 7.52-7.59 (m, 2H), 7.14-7.20 (m, 2H), 6.93-7.48 (m, 3H), 5.08 (q, J = 7.5 Hz, 1H), 1.17 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1,09 min MS (ESIpos): m/z = 483.2 [M + H]⁺ 45% yield 246

Intermediate 188; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.59-8.64 (m, 2H), 8.06-8.55 (m, 2H), 7.67-7.76 (m, 4H), 7.62 (s, 1H), 7.37-7.43 (m, 2H), 7.29 (s, 1H), 7.09 (t, J = 55.8 Hz, 1H), 5.02-5.13 (m, 1H), 1.17 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 0.85 min MS (ESIpos): m/z = 418.2 [M + H]⁺ 49% yield 247

Intermediate 189; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.03-8.53 (m, 3H), 8.00 (dd, J = 8.5, 2.4 Hz, 1H), 7.50-7.91 (m, 6H), 7.29 (s, 1H), 6.94-7.26 (m, 2H), 5.08 (q, J = 7.1 Hz, 1H), 1.18 (d, J = 7.6 Hz, 3H). LC-MS (method 2) Rt = 1.13 min MS (ESIpos): m/z = 484.5 [M + H]⁺ 53% yield 248

Intermediate 190; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.57 (d, J = 2.5 Hz, 1H), 8.31 (dd, J = 8.6, 2.5 Hz, 1H), 7.90-8.28 (m, 2H), 7.65-7.71 (m, 1H), 7.50-7.55 (m, 2H), 7.46 (d, J = 8.6 Hz, 1H), 7.33 (s, 1H), 6.92-6.97 (m, 2H), 5.03 (q, J = 6.8 Hz, 1H), 3.77 (s, 3H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 482.4 [M + H]⁺ 47% yield 249

Intermediate 191; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.56 (d, J = 2.5 Hz, 1H), 8.30 (dd, J = 8.7, 2.7 Hz, 3H), 7.69 (s, 1H), 7.51-7.57 (m, 2H), 7.43-7.50 (m, 3H), 7.34 (s, 1H), 5.03 (br d, J = 7.1 Hz, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.24 min MS (ESIpos): m/z = 486.3 [M + H]⁺ 23% yield 250

Intermediate 192; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.57 (d, J = 2.5 Hz, 1H), 8.30 (dd, J = 8.7, 2.7 Hz, 1H), 8.11 (br s, 2H), 7.68 (s, 1H), 7.57-7.62 (m, 2H), 7.45 (d, J = 9.1 Hz, 1H), 7.34 (s, 1H), 7.29 (t, J = 73.8 Hz, 1H), 7.19 (d, J = 8.6 Hz, 2H), 5.03 (br d, J = 6.6 Hz, 1H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.19 min MS (ESIpos): m/z = 518.3 [M + H]⁺ 31% yield 251

Intermediate 293; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.57 (d, J = 2.5 Hz, 1H), 8.43 (d, J = 2.3 Hz, 1H), 8.31 (dd, J = 8.7, 2.7 Hz, 3H), 8.04 (dd, J = 8.5, 2.4 Hz, 1H), 7.72 (t, J = 72.5 Hz, 1H), 7.70 (s, 1H), 7.46 (d, J = 9.1 Hz, 1H), 7.35 (s, 1H), 7.13 (d, J = 9.1 Hz, 1H), 5.03 (br d, J = 6.8 Hz, 1H), 1.20 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.21 min MS (ESIpos): m/z = 519.4 [M + H]⁺ 26% yield 252

Intermediate 194; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.47 (d, J = 2.5 Hz, 1H), 8.19 (dd, J = 8.6, 2.8 Hz, 3H), 7.75 (t, J = 72.2 Hz, 1H), 7.65 (s, 1H), 7.48-7.53 (m, 2H), 7.30 (s, 1H), 7.25 (d, J = 8.1 Hz, 1H), 6.92-6.96 (m, 2H), 5.05 (br d, J = 5.8 Hz, 1H), 3.76 (s, 3H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.09 min MS (ESIpos): m/z = 464.3 [M + H]⁺ 65% yield 253

Intermediate 195; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.46 (d, J = 2.3 Hz, 1H), 8.18 (dd, J = 8.7, 2.7 Hz, 3H), 7.74 (t, J = 72.5 Hz, 1H), 7.66 (s, 1H), 7.50-7.55 (m, 2H), 7.44-7.49 (m, 2H), 7.31 (s, 1H), 7.22-7.27 (m, 1H), 5.05 (br s, 1H), 1.18 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.19 min MS (ESIpos): m/z = 468.3 [M + H]⁺ 51% yield 254

Intermediate 196; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.47 (d, J = 2.5 Hz, 1H), 8.19 (dd, J = 8.7, 2.7 Hz, 3H), 7.75 (t, J = 72.5 Hz, 1H), 7.66 (s, 1H), 7.56-7.61 (m, 2H), 7.31 (s, 1H), 7.29 (t, J = 73.8 Hz, 1H), 7.25 (d, J = 8.6 Hz, 1H), 7.18 (d, J = 8.6 Hz, 2H), 5.05 (br s, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.14 min MS (ESIpos): m/z = 500.4 [M + H]⁺ 44% yield 255

Intermediate 197; rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 8.47 (d, J = 2.5 Hz, 1H), 8.41 (d, J = 2.0 Hz, 1H), 8.19 (dd, J = 8.6, 2.5 Hz, 3H), 8.03 (dd, J = 8.6, 2.5 Hz, 1H), 7.75 (t, J = 72.5 Hz, 1H), 7.72 (t, J = 72.3 Hz, 1H), 7.67 (s, 1H), 7.32 (s, 1H), 7.25 (d, J = 9.1 Hz, 1H), 7.12 (d, J = 8.6 Hz, 1H), 5.06 (br s, 1H), 1.19 (d, J = 7.4 Hz, 3H). LC-MS (method 2) Rt = 1.13 min MS (ESIpos): m/z = 501.5 [M + H]⁺ 56% yield 256

Intermediate 217, rac-2- bromo- propanamide ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.35 Hz, 3H), 5.05 (m, J = 6.34 Hz, 1H), 5.18 (s, 2H), 7.26 (s, 1H), 7.29-7.44 (m, 7H), 7.59 (s, 1H), 7.64 (dd, J = 8.87, 5.07 Hz, 2H), 7.80-7.85 (m, 1H), 7.85-7.91 (m, 1H), 7.94-8.36 (m, 2H), 8.40 (d, J = 1.77 Hz, 1H), 10.49 (s, 1H) RP-HPLC (method C) LC-MS (method 2) Rt = 1.16 min MS (ESIpos): m/z = 535.4 [M + H]⁺ 7% yield

Example 257 Rac-Ethyl 4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]benzoate

Ethyl 4-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]benzoate (14.2 g, 36.9 mmol, Intermediate 210) was suspended in DMF (300 mL) and treated with rac-2-bromo propionamide (7.9 g, 52 mmol) and potassium carbonate (9 g, 65 mmol). The reaction mixture was stirred at rt overnight and then treated with water. After 30 min the reaction mixture was extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness to give 15.47 g (33 mmol, 89% yield) of the title compound.

LC-MS (method 2): Rt=1.17 min; MS(ESIpos) m/z=457.3 [M+H]⁺

Example 258 Rac-Ethyl 4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-chloro-3-fluoro-anilino)thiazole-5-carbonyl]benzoate

Ethyl 4-[4-amino-2-(4-chloro-3-fluoro-anilino)thiazole-5-carbonyl]benzoate (1.24 g, 2.52 mmol, Intermediate 212) was suspended in DMF (18 mL) and treated with rac-2-bromo propionamide (765.5 mg, 5.04 mmol) and potassium carbonate (522 mg, 3.78 mmol). The reaction mixture was stirred for 3 h and then treated with water. After 30 min the reaction mixture was extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The crude product was purified by Biotage (method X) to give 1.02 g (82% yield) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.19 (m, J=8.0 Hz, 3H), 1.31 (t, J=7.1 Hz, 3H), 4.30 (q, J=7.10 Hz, 2H), 5.02 (m, 1H), 7.31 (s, 1H), 7.51 (dt, J=8.49, 1.20 Hz, 1H), 7.62 (m, 3H), 7.74 (m, 2H), 7.97 (m, 2H), 8.28 (m, 2H).

LC-MS (method 2): Rt=1.20 min; MS(ESIpos) m/z=491.2 [M+H]⁺

Example 259 Rac-Ethyl 2-[4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoate

rac-Ethyl 2-[4-[4-amino-2-(4-fluoroanilino)thiazole-5-carbonyl]phenoxy]-2-methyl-propanoate (1.01 g, 2.28 mmol, Intermediate 214) was suspended in DMF (20 mL) and treated with rac-2-bromo propionamide (519 mg, 3.4 mmol) and potassium carbonate (1.57 g, 11.4 mmol). The reaction mixture was stirred overnight at rt and then treated with water. After 30 min the reaction mixture was extracted three times with ethyl acetate. The combined organic phases were washed with brine, filtered through a water repellent filter circle (MN 617 WA) and evaporated to dryness. The crude product was purified by Biotage (method X) to give 926 mg (1.79 mmol, 78% yield) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.07 (t, J=7.1 Hz, 3H), 1.16 (d, J=7.35 Hz, 3H), 1.53 (s, 6H), 4.13 (q, J=7.1 Hz, 2H), 5.06 (m, 1H), 6.74 (m, 2H), 7.24 (s, 1H), 7.33 (t, J=8.74 Hz, 2H), 7.44 (m, 2H), 7.57 (s, 1H), 7.63 (m, 2H), 8.13 (m, 2H).

LC-MS (method 2): Rt=1.22 min; MS(ESIpos) m/z=515.5 [M+H]⁺

Example 260 Rac-4-[4-amino-2-(N-(2-amino-1-methyl-2-oxo-ethyl)-4-fluoro-anilino)thiazole-5-carbonyl]-N-cyclohexyl-benzamide

4-[(4-amino-2-{[(2RS)-1-amino-1-oxopropan-2-yl](4-fluorophenyl)amino}-1,3-thiazol-5-yl)carbonyl]benzoic acid (Intermediate 211, 75 mg, 0.175 mmol) and cyclohexane amine (35 mg, 0.35 mmol) were solved in 1 mL DMF and treated with HATU (133 mg, 0.35 mmol), N,N-diisopropylethylamine (68 mg, 0.53 mmol) and DMAP (1 mg, 9 μM), The reaction mixture was stirred at rt overnight, filtrated and purified by RP-HPLC (method D) to yield 48 mg (0.09 mmol, 53%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.06-1.14 (m, 1H), 1.16 (d, J=7.35 Hz, 3H), 1.20-1.36 (m, 4H), 1.55-1.64 (m, 1H), 1.68-1.76 (m, 2H), 1.76-1.83 (m, 2H), 3.66-3.79 (m, 1H), 5.01-5.12 (m, 1H), 7.25 (s, 1H), 7.32 (t, J=8.74 Hz, 2H), 7.52 (d, J=8.36 Hz, 2H), 7.58 (s, 1H), 7.64 (dd, J=8.87, 5.07 Hz, 2H), 7.78 (d, J=8.36 Hz, 2H), 7.98-8.12 (br s, 1H), 8.23 (d, J=7.86 Hz, 1H), 8.20-8.50 (br s, 1H).

LC-MS (method 2): Rt=1.15 min; MS(ESIpos) m/z=510.4 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 10, below, using the procedure as for Example 260.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) after precipitation, extraction or filtration of the reaction mixture if necessary.

Enantiomers were separated from their racemate by chiral HPLC using the column and solvent conditions stated.

TABLE 10 Examples 261-273 Example Chemical structure Starting number Compound name materials Analytics/purification/yield 261

Intermediate 211, propan- 2-amine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.11-1.19 (m, 9 H), 3.99- 4.11 (m, 1 H), 5.00-5.13 (m, 1 H), 7.25 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.53 (d, J = 8.62 Hz, 2 H), 7.58 (s, 1 H), 7.60-7.67 (m, 2 H), 7.79 (d, J = 8.36 Hz, 2 H), 7.94- 8.22 (m, 1 H), 8.24 (d, J = 7.86 Hz, 1 H), 8.27-8.48 (m, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.00 min MS (ESIpos): m/z = 470.7 [M + H]⁺ 73% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-fluoro- anilino)thiazole-5-carbonyl]-N- isopropyl-benzamide 262

Intermediate 211, 1- phenylmeth- anamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 4.46 (d, J = 6.08 Hz, 2 H), 5.01- 5.11 (m, 1 H), 7.21-7.28 (m, 2 H), 7.28-7.35 (m, 6 H), 7.53- 7.57 (m, 2 H), 7.58 (s, 1 H), 7.60- 7.66 (m, 2 H), 7.83-7.87 (m, 2 H), 8.09 (br s, 1 H), 8.38 (br s, 1 H), 9.07 (t, J = 5.96 Hz, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.10 min MS (ESIpos): m/z = 518.4 [M + H]⁺ 59% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-fluoro- anilino)thiazole-5-carbonyl]-N- benzyl-benzamide 263

Intermediate 211, S-1- amino- propan- 2-ol ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.04 (d, J = 6.34 Hz, 3 H), 1.13-1.19 (m, 3 H), 3.17 (td, J = 5.96, 1.52 Hz, 2 H), 3.70-3.81 (m, 1 H), 4.73 (d, J = 4.82 Hz, 1 H), 5.01-5.13 (m, 1 H), 7.26 (s, 1 H), 7.32 (t, J = 8.74 Hz, 2 H), 7.53 (d, J = 8.36 Hz, 2 H), 7.58 (s, 1 H), 7.61-7.66 (m, 2 H), 7.81 (d, J = 8.62 Hz, 2 H), 7.91-8.14 (m, 1 H), 8.17-8.38 (m, 1 H), 8.43 (t, J = 5.83 Hz, 1 H) RP-HPLC (method C basic) 4-[4-amino-2-(N-(2-amino-1- LC-MS (method 2) Rt = 0.83 min methyl-2-oxo-ethyl)-4-fluoro- MS (ESIpos): m/z = 486.6 [M + H]⁺ anilino)thiazole-5-carbonyl]-N- 73% yield [(2S)-2-hydroxypropyl]benzamide (mixture of stereoisomers) 264

Intermediate 211, 2- methoxy- ethanamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 3.25 (s, 3 H), 3.36-3.46 (m, 4 H), 5.00-5.12 (m, 1 H), 7.26 (s, 1 H), 7.29-7.36 (m, 2 H), 7.54 (d, J = 8.36 Hz, 2 H), 7.58 (s, 1 H), 7.63 (dd, J = 8.87, 5.07 Hz, 2 H), 7.80 (d, J = 8.36 Hz, 2 H), 7.90- 8.16 (m, 1 H), 8.17-8.47 (m, 1 H), 8.55 (t, J = 5.20 Hz, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 0.90 min MS (ESIpos): m/z = 486.6 [M + H]⁺ rac-4-[4-amino-2-(N-(2-amino-1- 68% yield methyl-2-oxo-ethyl)-4-fluoro- anilino)thiazole-5-carbonyl]-N-(2- methoxyethyl)benzamide 265

Intermediate 211, R-1- amino- propan- 2-ol ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.04 (d, J = 6.08 Hz, 3 H), 1.16 (d, J = 7.60 Hz, 3 H), 3.17 (td, J = 6.02, 1.65 Hz, 2 H), 3.70-3.80 (m, 1 H), 4.73 (d, J = 4.82 Hz, 1 H), 5.01-5.12 (m, 1 H), 7.25 (s, 1 H), 7.32 (t J = 8.74 Hz, 2 H), 7.51- 7.57 (m, 2 H), 7.58 (s, 1 H), 7.60- 7.67 (m, 2 H), 7.81 (d, J = 8.36 Hz, 2 H), 7.92-8.17 (m, 1 H), 8.18- 8.38 (m, 1 H), 8.43 (t, J = 5.70 Hz, 1 H) RP-HPLC (method C basic) 4-[4-amino-2-(N-(2-amino-1- LC-MS (method 2) Rt = 0.83 min methyl-2-oxo-ethyl)-4-fluoro- MS (ESIpos): m/z = 486.6 [M + H]⁺ anilino)thiazole-5-carbonyl]-N- 64% yield [(2R)-2-hydroxypropyl]benzamide (mixture of stereoisomers) 266

Intermediate 211, cyclopro- panamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 0.51-0.58 (m, 2 H), 0.64- 0.71 (m, 2 H), 1.16 (d, J = 7.35 Hz, 3 H), 2.82 (m, J = 4.06 Hz, 1 H), 5.06 (m, J = 7.10 Hz, 1 H), 7.25 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.52 (d, J = 8.36 Hz, 2 H), 7.58 (s, 1 H), 7.61-7.67 (m, 2 H), 7.73- 7.81 (m, 2H), 7.92-8.17 (m, 1 H), 8.18-8.40 (m, 1 H), 8.44 (d, J = 4.31 Hz, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 0.93 min rac-4-[4-amino-2-(N-(2-amino-1- MS (ESIpos): m/z = 468.5 [M + H]⁺ methyl-2-oxo-ethyl)-4-fluoro- 68% yield anilino)thiazole-5-carbonyl]-N- cyclopropyl-benzamide 267

Intermediate 211, cyclo- pentanamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.47-1.58 (m, 4 H), 1.63-1.73 (m, 2 H), 1.81-1.93 (m, 2 H), 4.14-4.26 (m, 1 H), 5.01-5.13 (m, 1 H), 7.25 (s, 1 H), 7.32 (t, J = 8.87 Hz, 2 H), 7.50-7.55 (m, 2 H), 7.58 (s, 1 H), 7.61-7.66 (m, 2 H), 7.75-7.82 (m, 2 H), 7.94- 8.11 (m, 1 H), 8.12-8.27 (m, 1 H), 8.31 (d, J = 7.35 Hz, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.08 min rac-4-[4-amino-2-(N-(2-amino-1- MS (ESIpos): m/z = 496.4 [M + H]⁺ methyl-2-oxo-ethyl)-4-fluoro- 47% yield anilino)thiazole-5-carbonyl]-N- cyclopentyl-benzamide 268

Intermediate 213, 2- phenoxy- ethanamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 3.58-3.65 (m, 2 H), 4.09 (t, J = 5.83 Hz, 2 H), 4.98-5.08 (m, 1 H), 6.90-6.97 (m, 3 H), 7.23- 7.34 (m, 3 H), 7.48-7.53 (m, 1 H), 7.55-7.59 (m, 2 H), 7.61- 7.66 (m, 1 H), 7.71-7.78 (m, 2 H), 7.81-7.87 (m, 2 H), 7.96- 8.57 (m, 2 H), 8.71-8.77 (m, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.15 min MS (ESIpos): m/z = 582.2 [M + H]⁺ 16% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-chloro-3- fluoro-anilino)thiazole-5-carbonyl]- N-(2-phenoxyethyl)benzamide 269

Intermediate 213, 2- (trifluoro- methoxy) ethanamine hydrochloride (1:1) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.55 (q, J = 5.35 Hz, 2 H), 4.18 (dd, J = 5.35 Hz, 2 H), 4.99-5.07 (m, 1 H), 7.30 (s, 1 H), 7.51 (dt, J = 8.62, 1.14 Hz, 1 H), 7.56-7.61 (m, 2 H), 7.63 (s, 1 H), 7.72-7.78 (m, 2 H), 7.79-7.88 (m, 2 H), 8.02-8.47 (m, 2 H), 8.78 (t, J = 5.58 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.11 min MS (ESIpos): m/z = 574.1 [M + H]⁺ 32% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-chloro-3- fluoro-anilino)thiazole-5-carbonyl]- N-[2-(trifluoromethoxy) ethyl]benzamide 270

Intermediate 213, 2- (difluoro- methoxy) ethanamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.48 (q, J = 5.58 Hz, 2 H), 3.91- 3.96 (m, 2 H), 4.98-5.09 (m, 1 H), 6.68 (t, J = 76 Hz, 1 H), 7.30 (s, 1 H), 7.48-7.53 (m, 1 H), 7.56- 7.60 (m, 2 H), 7.63 (s, 1 H), 7.70- 7.79 (m, 2 H), 7.79-7.86 (m, 2 H), 7.95-8.51 (m, 2 H), 8.66- 8.71 (m, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.02 min MS (ESIpos): m/z = 556.1 [M + H]⁺ 18% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-chloro-3- fluoro-anilino)thiazole-5-carbonyl]- N-[2- (difluoromethoxy)ethyl]benzamide 271

Intermediate 213, 2-tert- butoxy- ethanamine hydrochloride (1:1) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.12 (s, 9 H), 1.20 (d, J = 7.35 Hz, 3 H), 3.27-3.33 (m, 2 H), 3.37-3.43 (m, 2 H), 4.98- 5.07 (m, 1 H), 7.30 (s, 1 H), 7.47- 7.54 (m, 1 H), 7.54-7.59 (m, 2 H), 7.63 (s, 1 H), 7.71-7.78 (m, 2 H), 7.79-7.84 (m, 2 H), 7.97- 8.45 (m, 2 H), 8.52 (t, J = 5.58 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.02 min MS (ESIpos): m/z = 562.2 [M + H]⁺ 33% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-chloro-3- fluoro-anilino)thiazole-5-carbonyl]- N-(2-tert-butoxyethyl)benzamide 272

Intermediate 213, 2- methoxy- ethanamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.25 (s, 3 H), 3.37-3.47 (m, 4 H), 4.98-5.08 (m, 1 H), 7.31 (s, 1 H), 7.49-7.54 (m, 1 H), 7.54-7.59 (m, 2 H), 7.63 (s, 1 H), 7.71-7.78 (m, 2 H), 7.80-7.85 (m, 2 H), 7.97-8.46 (m, 2 H), 8.53-8.59 (m, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 0.95 min MS (ESIpos): m/z = 520.2 [M + H]⁺ 17% yield rac-4-[4-amino-2-(N-(2-amino-1- methyl-2-oxo-ethyl)-4-chloro-3- fluoro-anilino)thiazole-5-carbonyl]- N-(2-methoxyethyl)benzamide 273

Intermediate 215, 1-(4- chlorophenyl) methanamine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.45 (s, 6 H), 4.23 (d, J = 6.08 Hz, 2 H), 5.00-5.13 (m, 1 H), 6.77- 6.82 (m, 2 H), 7.13-7.19 (m, 2 H), 7.24 (s, 1 H), 7.27-7.36 (m, 4 H), 7.38-7.45 (m, 2 H), 7.57 (s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.80-8.57 (m, 2 H), 8.68 (t, J = 6.08 Hz, 1 H) RP-HPLC (method C basic) LC-MS (method 2) Rt = 1.23 min MS (ESIpos): m/z = 610.4 [M + H]⁺ 77% yield rac-2-[4-[4-amino-2-(N-(2-amino- 1-methyl-2-oxo-ethyl)-4-fluoro- anilino)thiazole-5- carbonyl]phenoxy]-N-[(4- chlorophenyl)methyl]-2-methyl- propanamide

Example 274 Rac-2-(N-[4-amino-5-(6-bromopyridine-3-carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide

[4-amino-2-(4-fluoroanilino)-1,3-thiazol-5-yl](6-bromopyridin-3-yl)methanone (2.52 g, 6.4 mmol, Intermediate 218) was suspended in DMF (100 mL) and treated with rac-2-bromo propionamide (1.46 g, 9.6 mmol) and potassium carbonate (4.43 g, 32 mmol). The reaction mixture was stirred overnight at rt and then treated with water. After 30 min the precipitate was filtered off, washed with water and dried in vacuo to give 2.2 g (4.4 mmol, 69% yield) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.16 (d, J=7.35 Hz, 3H), 5.05 (m, 1H), 7.27 (m, 1H), 7.34 (t, J=8.87 Hz, 2H), 7.65 (m, 4H), 7.80 (dd, J=8.24, 2.41 Hz, 1H), 8.25 (m, 2H), 8.48 (d, J=2.03 Hz, 1H).

LC-MS (method 2): Rt=1.08 min; MS(ESIpos) m/z=466.1 [M+H]⁺

Example 275 Rac-2-(N-[4-amino-5-[6-[4-(trifluoromethyl)-1-piperidyl]pyridine-3-carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide

2-(N-[4-amino-5-(6-bromopyridine-3-carbonyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (50 mg, 0.11 mmol, Example 274), 4-(trifluoromethyl)piperidine (25 mg, 0.16 mmol), tetrabutylammonium iodide (4 mg, 0.01 mmol) and potassium carbonate (18 mg, 0.13 mmol) were suspended in DMSO and stirred overnight at 50° C. The reaction mixture was filtrated and purified by RP-HPLC (method D) to yield 24 mg (0.04 mmol, 41%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.17 (d, J=7.35 Hz, 3H), 130-1.43 (m, 2H), 1.84 (br d, J=11.15 Hz, 2H), 256-2.65 (m, 1H), 2.87 (td, J=12.86, 2.15 Hz, 2H), 4.42-4.51 (m, 2H), 5.00-5.11 (m, 1H), 6.84 (d, J=8.87 Hz, 1H), 7.25 (s, 1H), 7.35 (t, J=8.87 Hz, 2H), 7.58 (s, 1H), 7.61-7.72 (m, 3H), 7.80-8.26 (m, 2H), 8.31 (d, J=2.28 Hz, 1H).

LC-MS (method 2): Rt=1.25 min; MS(ESIpos) m/z=537.5 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 11, below, using the procedure as for Example 275.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) after precipitation, extraction or filtration of the reaction mixture if necessary.

Enantiomers were separated from their racemate by chiral HPLC using the column and solvent conditions stated.

TABLE 11 Examples 276-285.2 Example Chemical structure Starting number Compound name materials Analytics/purification/yield 276

Example 274, 4- methyl- piperidine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 0.89 (d, J = 6.08 Hz, 3 H), 0.97-1.09 (m, 2 H), 1.17 (d, J = 7.35 Hz, 3 H), 1.57-1.68 (m, 3 H), 2.76-2.87 (m, 2 H), 4.32 (br d, J = 13.18 Hz, 2 H), 5.01-5.10 (m, 1 H), 6.78 (d, J = 8.87 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.87 Hz, 2 H), 7.58 (br s, 1 H), 7.62-7.69 (m, 3 H), 7.86-8.24 (m, 2 H), 8.29 (d, J = 2.28 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.28 min rac-2-(N-[4-amino-5-[6-(4-methyl- MS (ESIpos): m/z = 438.3 [M + H]⁺ 1-piperidyl)pyridine-3- 56% yield carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 277

Example 274, 4-(oxetan-3- yl)piperidine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 0.94 (br dd, J = 12.29, 3.42 Hz, 2 H), 1.16 (d, J = 7.35 Hz, 3 H), 1.61 (br d, J = 11.15 Hz, 2 H), 1.81-1.94 (m, 1 H), 2.63-2.73 (m, 1 H), 2.79-2.89 (m, 2 H), 4.30-4.40 (m, 4 H), 4.59 (dd, J = 7.86, 6.08 Hz, 2 H), 5.05 (q, J = 7.10 Hz, 1 H), 6.79 (d, J = 8.87 Hz, 1 H), 7.25 (s, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.58 (s, 1 H), 7.60-7.70 (m, 3 H), 7.80-8.23 (m, 2 H), 8.29 (d, J = 2.53 Hz, 1 H) rac-2-(N-[4-amino-5-[6-[4-(oxetan- RP-HPLC (method D basic) 3-yl)-1-piperidyl]pyridine-3- LC-MS (method 2) Rt = 1.06 min carbonyl]thiazol-2-yl]-4-fluoro- MS (ESIpos): m/z = 525.5 [M + H]⁺ anilino)propanamide 60% yield 278

Example 274, N- methyl- methanamine ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.25 Hz, 3 H), 3.03 (s, 6 H), 5.01-5.09 (m, 1 H), 6.61 (d, J = 8.83 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.83 Hz, 2 H), 7.58 (s, 1 H), 7.63-7.70 (m, 3 H), 7.82-8.26 (m, 2 H), 8.30 (d, J = 2.21 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 429.5 [M + H]⁺ 51% yield rac-2-(N-[4-amino-5-[6- (dimethylamino)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 279

Example 274, 4,4- dimethyl- piperidine hydrochloride (1:1) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 0.95 (s, 6 H), 1.17 (d, J = 7.35 Hz, 3 H), 1.28-1.34 (m, 4 H), 3.52-3.60 (m, 4 H), 5.00- 5.12 (m, 1 H), 6.78 (d, J = 9.13 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.87 Hz, 2 H), 7.55-7.61 (m, 1 H), 7.62-7.69 (m, 3 H), 7.80-8.24 (m, 2 H), 8.29 (d, J = 2.53 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.37 min MS (ESIpos): m/z = 497.5 [M + H]⁺ 59% yield rac-2-(N-[4-amino-5-[6-(4,4- dimethyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 280

Example 274, 3- azabicyclo [3.2.1]octane hydrochloride (1:1) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 1.37-1.46 (m, 2 H), 1.50-1.56 (m, 2 H), 1.56-1.67 (m, 2 H), 2.26-2.32 (m, 2 H), 2.85 (d, J = 10.39 Hz, 2 H), 3.93 (br d, J = 10.65 Hz, 2 H), 4.99-5.10 (m, 1 H), 6.67 (d, J = 8.87 Hz, 1 H), 7.25 (s, 1 H), 7.34 (t, J = 8.87 Hz, 2 H), 7.58 (s, 1 H), 7.61-7.69 (m, 3 H), 7.75-8.25 (m, 2 H), 8.29 (d, J = 2.53 Hz, 1 H) RP-HPLC (method D basic) rac-2-(N-[4-amino-5-[6-(3- LC-MS (method 2) Rt = 1.29 min azabicyclo[3.2.1]octan-3- MS (ESIpos): m/z = 495.3 [M + H]⁺ yl)pyridine-3-carbonyl]thiazol-2- 60% yield yl]-4-fluoro-anilino)propanamide 281

Example 274, 3,5- dimethyl- piperidine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 0.87 (d, J = 6.59 Hz, 6 H), 1.17 (d, J = 7.60 Hz, 3H), 1.44- 1.57 (m, 2 H), 1.75 (br d, J = 12.42 Hz, 1 H), 2.25-2.34 (m, 2 H), 4.34 (br d, J = 9.89 Hz, 2 H), 5.01- 5.12 (m, 1 H), 6.80 (d, J = 8.87 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.74 Hz, 2 H), 7.58 (s, 1 H), 7.62-7.70 (m, 3 H), 7.81-8.26 (m, 2 H), 8.29 (d, J = 2.53 Hz, 1 H), 0.78 (q, J = 12 Hz, 1 H) RP-HPLC (method D basic) rac-2-(N-[4-amino-5-[6-(3,5- LC-MS (method 2) Rt = 1.37 min dimethyl-1-piperidyl)pyridine-3- MS (ESIpos): m/z = 497.3 [M + H]⁺ carbonyl]thiazol-2-yl]-4-fluoro- 59% yield anilino)propanamide 282

Example 274, 3- azabicyclo [3.1.0]hexane hydrochloride (1:1) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 0.13 (d, J = 4.31 Hz, 1 H), 0.72 (td, J = 7.67, 4.69 Hz, 1 H), 1.16 (d, J = 7.35 Hz, 3H), 1.63- 1.69 (m, 2 H), 3.34-3.41 (m, 2 H), 3.63 (br d, J = 10.39 Hz, 2 H), 5.05 (br d, J = 7.35 Hz, 1 H), 6.42 (d, J = 8.62 Hz, 1 H), 7.25 (s, 1 H), 7.34 (t, J = 8.74 Hz, 2 H), 7.58 (s, 1 H), 7.60-7.70 (m, 3 H), 7.83- 8.22 (m, 2 H), 8.27 (d, J = 2.28 Hz, 1 H) RP-HPLC (method D basic) rac-2-(N-[4-amino-5-[6-(3- LC-MS (method 2) Rt = 1.12 min azabicyclo[3.1.0]hexan-3- MS (ESIpos): m/z = 467.3 [M + H]⁺ yl)pyridine-3-carbonyl]thiazol-2- 72% yield yl]-4-fluoro-anilino)propanamide 283

Example 274, piperidine ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.35 Hz, 3 H), 1.45-1.55 (m, 4 H), 1.56-1.64 (m, 2 H), 3.53-3.59 (m, 4 H), 5.00-5.10 (m, 1 H), 6.78 (d, J = 9.12 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.74 Hz, 2 H), 7.58 (s, 1 H), 7.62-7.70 (m, 3 H), 7.80- 8.23 (m, 2 H), 8.29 (d, J = 2.28 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.22 min MS (ESIpos): m/z = 469.5 [M + H]⁺ rac-2-(N-[4-amino-5-[6-(1- 100% yield piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 284

Example 274, 4,4- difluoro- piperidine hydrochloride (1:1) ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.60 Hz, 3 H), 1.90-2.03 (m, 4 H), 3.69-3.76 (m, 4 H), 5.00-5.11 (m, 1 H), 6.93 (d, J = 9.13 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.74 Hz, 2 H), 7.58 (s, 1 H), 7.62-7.72 (m, 3 H), 7.80-8.26 (m, 2 H), 8.31-8.34 (m, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.09 min MS (ESIpos): m/z = 466.2 [M + H]⁺ 26% yield rac-2-(N-[4-amino-5-[6-(4,4- difluoro-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 285

Example 274, 4- methyl- piperidine-4- carbonitrile hydrochloride (1:1) ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.25 Hz, 3 H), 1.35 (s, 3 H), 1.49 (td, J = 12.77, 4.10 Hz, 2 H), 1.90 (br d, J = 13.87 Hz, 2 H), 2.97-3.05 (m, 2 H), 4.36 (br d, J = 13.87 Hz, 2 H), 5.01- 5.10 (m, 1 H), 6.86 (d, J = 8.83 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.83 Hz, 2 H), 7.58 (s, 1 H), 7.63-7.70 (m, 3 H), 7.78-8.29 (m, 2 H), 8.31 (d, J = 2.21 Hz, 1 H) RP-HPLC (method D basic) LC-MS (method 2) Rt = 1.04 min rac-2-(N-[4-amino-5-[6-(4-cyano- MS (ESIpos): m/z = 508.8 [M + H]⁺ 4-methyl-1-piperidyl)pyridine-3- 64% yield carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 285.1 (R)-2-(N-[4-amino-5-[6-(4-cyano- and 4-methyl-1-piperidyl)pyridine-3- 285.2 carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide and (S)-2-(N- [4-amino-5-[6-(4-cyano-4-methyl- 1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide 285.1

Example 285 ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.25 Hz, 3 H), 1.35 (s, 3 H), 1.49 (td, J = 12.77, 4.10 Hz, 2 H), 1.90 (br d, J = 13.87 Hz, 2 H), 2.97-3.05 (m, 2 H), 4.36 (br d, J = 13.87 Hz, 2 H), 5.01- 5.10 (m, 1 H), 6.86 (d, J = 8.83 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.83 Hz, 2 H), 7.58 (s, 1 H), 7.63-7.70 (m, 3 H), 7.78-8.29 (m, 2 H), 8.31 (d, J = 2.21 Hz, 1 H) LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 508.8 [M + H]⁺ 2-(N-[4-amino-5-[6-(4-cyano-4- 39% yield methyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (enantiomer 1) Chiral HPLC Example 285.1 HPLC separation of rac-2-(N-[4-amino-5-[6-(4-cyano-4-methyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide (98 mg, 0.19 mmol, Example 285) on a chiral column gave 38 mg (39% yield) of 2-(N-[4-amino-5-[6-(4-cyano-4-methyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 1. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10 μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.37 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3 μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 285.2

Example 285 ¹H NMR (500 MHz, DMSO-d₆) δ ppm = 1.17 (d, J = 7.25 Hz, 3 H), 1.35 (s, 3 H), 1.49 (td, J = 12.77, 4.10 Hz, 2 H), 1.90 (br d, J = 13.87 Hz, 2 H), 2.97-3.05 (m, 2 H), 4.36 (br d, J = 13.87 Hz, 2 H), 5.01- 5.10 (m, 1 H), 6.86 (d, J = 8.83 Hz, 1 H), 7.25 (s, 1 H), 7.35 (t, J = 8.83 Hz, 2 H), 7.58 (s, 1 H), 7.63-7.70 (m, 3 H), 7.78-8.29 (m, 2 H), 8.31 (d, J = 2.21 Hz, 1 H) LC-MS (method 2) Rt = 1.04 min MS (ESIpos): m/z = 508.8 [M + H]⁺ 2-(N-[4-amino-5-[6-(4-cyano-4- 42% yield methyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro- anilino)propanamide (enantiomer 2) Chiral HPLC Example 285.2 HPLC separation of rac-2-(N-[4-amino-5-[6-(4-cyano-4-methyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide (98 mg, 0.19 mmol, Example 285) on a chiral column gave 41 mg (42% yield) of 2-(N-[4-amino-5-[6-(4-cyano-4-methyl-1-piperidyl)pyridine-3- carbonyl]thiazol-2-yl]-4-fluoro-anilino)propanamide, enantiomer 2. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SC 10 μ, 250 × 50; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.22 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3 μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm

Example 286 2-(N-[4-amino-5-(4-hydroxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (Single Enantiomer)

2-(N-[4-amino-5-(4-benzyloxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide (enantiomer 2, example 170.2, 41 mg, 0.084 mmol) was solved in ethanol (1 mL). Under nitrogen, Pd/C (133 mg) was added and the reaction mixture was purged with H₂ and stirred under atmospheric H₂ for 5 h at rt. The mixture was filtrated via Celite and the solvent was evaporated under reduced pressure. The residue was purified by RP-HPLC (method B) to yield 22 mg (0.05 mmol, 61%) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.16 (d, J=7.35 Hz, 3H), 4.99-5.10 (m, 1H), 6.70-6.75 (m, 2H), 7.24 (s, 1H), 7.30-7.40 (m, 4H), 7.57 (s, 1H), 7.61-7.66 (m, 2H), 7.84-8.32 (m, 2H), 9.90 (br s, 1H).

LC-MS (method 2): Rt=0.68 min; MS(ESIpos) m/z=401.3 [M+H]⁺

The following examples were prepared from the starting materials stated in Table 12, below, using the procedure as for Example 286.

The crude product was either purified by RP-HPLC (methods A-D depending on polarity) or by preparative flash chromatography (methods X, Y or Z depending on polarity) after precipitation, extraction or filtration of the reaction mixture if necessary.

Enantiomers were separated from their racemate by chiral HPLC using the column and solvent conditions stated.

TABLE 12 Examples 287-291 Example Chemical structure Starting number Compound name materials Analytics/purification/yield 287

Example 165.1 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 4.96-5.06 (m, 1 H), 6.72 (d, J = 8.62 Hz, 2 H), 7.28 (s, 1 H), 7.37-7.43 (m, 2H), 7.48-7.54 (m, 1 H), 7.55-7.64 (m, 2 H), 7.72-7.83 (m, 1 H), 7.83-8.25 (m, 2 H) RP-HPLC (method B basic) LC-MS (method 2) Rt = 0.68 min MS (ESIpos): m/z = 419.3 [M + H]⁺ 49% yield 2-(N-[4-amino-5-(4- hydroxybenzoyl)thiazol-2- yl]-3,4-difluoro- anilino)propanamide (enantiomer 1) 288

Example 165.2 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 4.96-5.06 (m, 1 H), 6.72 (d, J = 8.62 Hz, 2 H), 7.28 (s, 1 H), 7.37-7.43 (m, 2 H), 7.48-7.54 (m, 1 H), 7.55-7.64 (m, 2 H), 7.72-7.83 (m, 1 H), 7.83-8.25 (m, 2 H) RP-HPLC (method B basic) LC-MS (method 2) Rt = 0.68 min MS (ESIpos): m/z = 419.3 [M + H]⁺ 43% yield 2-(N-[4-amino-5-(4- hydroxybenzoyl)thiazol-2- yl]-3,4-difluoro- anilino)propanamide (enantiomer 2) 289

Example 166.1 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 4.97-5.06 (m, 1 H), 6.72-6.78 (m, 2H), 7.29 (s, 1 H), 7.38-7.46 (m, 2 H), 7.51 (dt, J = 8.49, 1.20 Hz, 1 H), 7.62 (s, 1 H), 7.71-7.79 (m, 2 H), 7.83-8.26 (m, 2 H), 9.93 (s, 1 H) Biotage (method X) LC-MS (method 2) Rt = 0.76 min MS (ESIpos): m/z = 435.2 [M + H]⁺ 21% yield 2-(N-[4-amino-5-(4- hydroxybenzoyl)thiazol-2- yl]-4-chloro-3-fluoro- anilino)propanamide (enantiomer 1) 290

Example 166.2 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.19 (d, J = 7.35 Hz, 3 H), 4.97-5.06 (m, 1 H), 6.72-6.78 (m, 2 H), 7.29 (s, 1 H), 7.38-7.46 (m, 2 H), 7.51 (dt, J = 8.49, 1.20 Hz, 1 H), 7.62 (s, 1 H), 7.71-7.79 (m, 2 H), 7.83-8.26 (m, 2 H), 9.93 (s, 1 H) Biotage (method X) LC-MS (method 2) Rt = 0.76 min MS (ESIpos): m/z = 435.2 [M + H]⁺ 2-(N-[4-amino-5-(4- 27% yield hydroxybenzoyl)thiazol-2- yl]-4-chloro-3-fluoro- anilino)propanamide (enantiomer 2) 291

Example 182.2 ¹H NMR (400 MHz, DMSO-d₆) δ ppm = 1.14 (d, J = 7.35 Hz, 3 H), 4.96-5.13 (m, 1 H), 6.80 (d, J = 8.87 Hz, 2 H), 7.14-7.21 (m, 3 H), 7.27 (t, J = 72 Hz, 1H), 7.32 (d, J = 8.62 Hz, 2 H), 7.49 (s, 1 H), 7.52-7.58 (m, 2 H), 7.78-8.55 (m, 2 H), 9.85 (s, 1 H) RP-HPLC (method B basic) LC-MS (method 2) Rt = 0.68 min MS (ESIpos): m/z = 449.3 [M + H]⁺ 47% yield 2-(N-[4-amino-5-[4- (difluoromethoxy)benzoyl] thiazol-2-yl]-4-hydroxy- anilino)propanamide (single enantiomer)

Example 292 Rac-2-(N-[4-amino-5-[4-(2-hydroxyethoxy)benzoyl]thiazol-2-yl]-4-fluoro-anilino)propanamide

[4-amino-2-(4-fluoroanilino)thiazol-5-yl]-[4-(2-hydroxyethoxy)phenyl]methanone (Intermediate 209, 74 mg, 0.2 mmol) was suspended in DMF (4 mL) and treated with rac-2-bromopropamide (30 mg, 0.2 mmol) and potassium carbonate (137 mg, 0.2 mmol). The reaction mixture was stirred at rt for 4 days. The filtrate was purified by RP-HPLC (method C, basic) to give 47 mg (53% yield) of the title compound.

¹H NMR (400 MHz, DMSO-d₆) δ ppm=1.15 (d, J=7.35 Hz, 3H) 3.64-3.71 (m, 2H) 398 (t, J=4.94 Hz, 2H) 4.87-4.93 (m, 1H) 5.00-5.11 (m, 1H) 6.88-6.95 (m, 2H) 7.23 (s, 1H) 7.30-7.36 (m, 2H) 7.44-7.49 (m, 2H) 7.56-7.60 (m, 1H) 7.61-7.66 (m, 2H) 7.74-8.34 (m, 2H) 9.02-9.07 (m, 1H).

LC-MS (method 1): Rt=0.84 min; MS(ESIpos) m/z=445.6 [M+H]⁺.

EXPERIMENTAL SECTION—DETERMINATION OF ABSOLUTE STEREOCHEMISTRY BY MEANS OF X-RAY-ANALYSIS Determination of the Absolute Configuration of Example 49.2 (R)-2-(N-[4-amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2-yl]-3,4-difluoro-anilino)propanamide

The crystallographic data of Example 49.2 as well as a figure depicting the thermal ellipsoids and numbering of the structure, are shown in Table 13 and FIG. 6 . Colorless crystals of Example 49.2 were obtained by slow evaporation of an ethanol solution. A single crystal was mounted on a cryoloop using a protective oil. Single-crystal X-ray diffraction data were collected at 100 K on a Rigaku XtaLAB Synergy S system with a kappa goniometer and a HyPix-6000HE hybrid photon detector using Cu X-ray radiation (CuKα, =1.54178 Å). Data were integrated using the program CrysAlisPRO. SHELXM was used for structure solution and SHELXL was used for full-matrix least-squares refinement on F2. In the asymmetric unit two molecules of Example 49.2 and two water molecules are present. The di-fluorinated phenyl rings in both molecules are disordered via a 180° rotation of the ring systems. The occupancies for the alternative positions were refined to 0.25/0.75 in Molecule A and 0.35/0.65 in Molecule B, respectively. All non-hydrogen atoms were refined anisotropically. All hydrogen atoms were added in calculated positions and refined riding on their resident atoms. Hydrogens attached to the nitrogen atoms in 49.2 as well as at the water molecules were located in the difference Fourier map and placed manually. The isotropic temperature factors of the hydrogen atoms were refined as 1.2 and 1.5 times the size of the temperature factors of the corresponding heavy atoms, respectively. The absolute stereochemistry (R-configuration) could be assigned unambiguously with a Flack Parameter of 0.008 (7). The program XP was used for molecular representations.

TABLE 13 Crystal data and structure refinement for Example 49.2 Identification code Example 49.2 Empirical formula C40 H36 F8 N8 O8 S2 Formula weight 972.89 Temperature 100(2) K Wavelength 1.54184 Å Crystal system Triclinic Space group P1 Unit cell dimensions a = 7.59740(10) Å a = 78.1410(10)°. b = 9.28630(1) Å b = 89.0340(10)°. c = 15.1835(2) Å g = 83.8330(10)°. Volume 1042.28(2) Å³ Z 1 Density (calculated) 1.550 Mg/m³ Absorption coefficient 2.049 mm⁻¹ F(000) 500 Crystal size 0.050 × 0.040 × 0.160 mm³ Theta range for data collection 2.974 to 68.246°. Index ranges −9 <= h <= 9, −11 <= k <= 11, −18 <= l <= 18 Reflections collected 74868 Independent reflections 7441 (R(int) = 0.0428] Completeness to theta = 67.684° 99.9% Refinement method Full-matrix least-squares on F² Data/restraints/parameters 7441/153/651 Goodness-of-fit on F² 1.044 Final R indices (I > 2sigma(I)] R1 = 0.0425, wR2 = 0.1062 R indices (all data) R1 = 0.0437, wR2 = 0.1072 Absolute structure parameter 0.008(7) Extinction coefficient n/a Largest diff. peak and hole 0.879 and −0.502 e.Å⁻³

Determination of the Absolute Configuration of Example 59.1 (R)-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-chloro-3-fluoro-anilino)propanamide

The crystallographic data of Example 59.1 as well as a figure depicting the thermal ellipsoids and numbering of the structure, are shown in Table 14 and FIG. 7 . Colorless crystals of Example 59.1 were obtained by slow evaporation from a toluene solution. A single crystal was mounted on a cryoloop using a protective oil. Single-crystal X-ray diffraction data were collected at 100 K on a Rigaku XtaLAB Synergy S system with a kappa goniometer and a HyPix-6000HE hybrid photon detector using Cu X-ray radiation (CuKα, =1.54178 Å). Data were integrated using the program CrysAlisPRO. SHELXM was used for structure solution and SHELXL was used for full-matrix least-squares refinement on F2. In the asymmetric unit four molecules of Example 59.1 and one disordered toluene molecules are present. The fluorinated phenyl ring systems in three of the four molecules are disordered via a 180° rotation of the ring systems. The occupancies for the alternative positions were refined to 0.30/0.70 in Molecule B, 0.20/0.80 in Molecule C and 0.15/0.85 in Molecule D, respectively. The toluene solvent molecule is disordered over a pseudo 2-fold axis and the occupancies for both alternative positions refined with a ratio of 0.45/0.55. All non-hydrogen atoms were refined anisotropically. Hydrogen atoms were added in calculated positions and refined riding on their resident atoms. Hydrogens attached to amine and amide nitrogen atoms were either located in the difference Fourier map and placed manually or were refined using the riding model. The isotropic temperature factors of the hydrogen atoms were refined as 1.2 and 1.5 times the size of the temperature factors of the corresponding heavy atoms, respectively. The absolute stereochemistry (R-configuration) could be assigned unambiguously with a Flack Parameter of 0.015 (11). The program XP was used for molecular representations.

TABLE 14 Crystal data and structure refinement for Example 59.1 Identification code 59.1 Empirical formula C20 H18 N4 O3 F Cl S + 0.25 (C7 H8) Formula weight 448.9 + 23.0 Temperature 100(2) K Wavelength 1.54178 Å Crystal system Triclinic Space group P1 Unit cell dimensions a = 8.9781(2) Å a = 103.099(2)°. b = 13.3867(3) Å b = 92.827(2)°. c = 18.9350(3) Å g = 101.417(2)°. Volume 2162.07(8) Å³ Z 4 Density (calculated) 1.450 Mg/m³ Absorption coefficient 2.827 mm⁻¹ F(000) 978 Crystal size 0.070 × 0.060 × 0.005 mm³ Theta range for data collection 2.407 to 68.401°. Index ranges −10 <= h <= 10, −16 <= k <= 16, −22 <= l <= 22 Reflections collected 76934 Independent reflections 15381 [R(int) = 0.0650] Completeness to theta = 67.679° 100.0% Refinement method Full-matrix least-squares on F² Data/restraints/parameters 15381/1400/1272 Goodness-of-fit on F² 1.003 Final R indices (I > 2sigma(I)] R1 = 0.0496, wR2 = 0.1120 R indices (all data) R1 = 0.0605, wR2 = 0.1185 Absolute structure parameter 0.015(11) Extinction coefficient n/a Largest diff. peak and hole 0.462 and −0.430 e.Å⁻³

Determination of the Absolute Configuration of Example 61.2 (R)-2-(N-(4-amino-5-benzoyl-thiazol-2-yl)-4-fluoro-anilino)propanamide

The crystallographic data of Example 61.2 as well as a figure depicting the thermal ellipsoids and numbering of the structure, are shown in Table 15 and FIG. 8 . Colorless crystals of Example 61.2 were obtained by slow evaporation from an acetonitrile solution. A single crystal was mounted on a cryoloop using a protective oil. Single-crystal X-ray diffraction data were collected at 100 K on a Rigaku XtaLAB Synergy S system with a kappa goniometer and a HyPix-6000HE hybrid photon detector using Cu X-ray radiation (CuKα, =1.54178 Å). Data were integrated using the program CrysAlisPRO. SHELXM was used for structure solution and SHELXL was used for full-matrix least-squares refinement on F2. In the asymmetric unit eight molecules of Example 61.2 and two water molecules are present. All non-hydrogen atoms were refined anisotropically. All hydrogen atoms were added in calculated positions and refined riding on their resident atoms. Hydrogens attached to amine and amide nitrogen atoms were either located in the difference Fourier map and placed manually or were refined using the riding model. The isotropic temperature factors of the hydrogen atoms were refined as 1.2 and 1.5 times the size of the temperature factors of the corresponding heavy atoms, respectively. The absolute stereochemistry (R-configuration) could be assigned unambiguously with a Flack Parameter of 0027 (11). The program XP was used for molecular representations.

TABLE 15 Crystal data and structure refinement for Example 61.2 Identification code Example 61.2 Empirical formula C19 H20 F N4 O2 S + 0.1875 H2 O Formula weight 390.45 Temperature 100(2) K Wavelength 1.54178 Å Crystal system Tetragonal Space group P4(1) Unit cell dimensions a = 17.135 Å a = 90°. b = 17.135 Å b = 90°. c = 49.3911(2) Å g = 90°. Volume 14502.48(6) Å³ Z 32 Density (calculated) 1.431 Mg/m³ Absorption coefficient 1.886 mm⁻¹ F(000) 6544 Crystal size 0.3 × 0.2 × 0.1 mm³ Theta range for data collection 2.58 to 77.25°. Index ranges −14 <= h <= 15, 0 <= k <= 21, −59 <= l <= 61 Reflections collected 178451 Independent reflections 26790 [R(int) = 0.0513] Completeness to theta = 77.25° 91.2% Refinement method Full-matrix least-squares on F² Data/restraints/parameters 26790/1889/2043 Goodness-of-fit on F² 1.315 Final R indices (I > 2sigma(I)] R1 = 0.0508, wR2 = 0.1276 R indices (all data) R1 = 0.0538, wR2 = 0.1292 Absolute structure parameter 0.027(11) Largest diff. peak and hole 0.475 and −0.425 e.Å⁻³

Determination of the Absolute Configuration of Example 62.2 (R)-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide

The crystallographic data of Example 62.2 as well as a figure depicting the thermal ellipsoids and numbering of the structure, are shown in Table 16 and FIG. 9 . Colorless crystals of Example 62.2 were already present in the purified sample. A single crystal was mounted on a cryoloop using a protective oil. Single-crystal X-ray diffraction data were collected at 100 K on a Rigaku XtaLAB Synergy S system with a kappa goniometer and a HyPix-6000HE hybrid photon detector using Cu X-ray radiation (CuKα, =1.54178 Å). Data were integrated using the program CrysAlisPRO. SHELXM was used for structure solution and SHELXL was used for full-matrix least-squares refinement on F2. In the asymmetric unit two molecules of Example 62.2 are present. All non-hydrogen atoms were refined anisotropically. All hydrogen atoms were added in calculated positions and refined riding on their resident atoms. Hydrogens attached to the nitrogen atoms were located in the difference Fourier map and placed manually. The isotropic temperature factors of the hydrogen atoms were refined as 1.2 and 1.5 times the size of the temperature factors of the corresponding heavy atoms, respectively. The absolute stereochemistry (R-configuration) could be assigned unambiguously with a Flack Parameter of 0.004 (3). The program XP was used for molecular representations.

TABLE 16 Crystal data and structure refinement for Example 62.2 Identification code Example 62.2 Empirical formula C40 H38 F2 N8 O6 S2 Formula weight 828.90 Temperature 100(2) K Wavelength 1.54184 Å Crystal system Monoclinic Space group I2 Unit cell dimensions a = 14.128 Å a = 90°. b = 13.01730(10) Å b = 92.60°. c = 21.74910(10) Å g = 90°. Volume 3995.63(4) Å³ Z 4 Density (calculated) 1.378 Mg/m³ Absorption coefficient 1.778 mm⁻¹ F(000) 1728 Crystal size 0.100 × 0.070 × 0.020 mm³ Theta range for data collection 3.656 to 77.353°. Index ranges −17 <= h <= 17, −16 <= k <= 15, −27 <= l <= 27 Reflections collected 75900 Independent reflections 8071 [R(int) = 0.0302] Completeness to theta = 67.684° 100.0% Refinement method Full-matrix least-squares on F² Data/restraints/parameters 8071/1/551 Goodness-of-fit on F² 1.065 Final R indices [I > 2sigma(I)] R1 = 0.0226, wR2 = 0.0591 R indices (all data) R1 = 0.0227, wR2 = 0.0592 Absolute structure parameter −0.004(3) Extinction coefficient n/a Largest diff. peak and hole 0.151 and −0.239 e.Å⁻³

EXPERIMENTAL SECTION—BIOLOGICAL ASSAYS AND BIOLOGICAL DATA

Table 17, below, lists the abbreviations used in this paragraph and in the Assays section as far as they are not explained within the text body. Other abbreviations have their meanings customary per se to the skilled person.

TABLE 17 Abbreviations nL nanoliter μL microliter mL milliliter nM nanomolar μM micromolar mM millimolar min minute(s) s second(s) kDa kilodalton MW molecular weight cAMP cyclic adenosine monophosphat ADP adenosine diphosphate ATP adenosine triphosphate FCS fetal calf serum FBS fetal bovine serum PBS phosphate buffered saline RPMI Roswell Park Memorial Institute ACK lysing buffer ammonium-chloride-potassium lysis buffer DMEM Dulbecco's Modified Eagle's Medium HEPES 2-[4-(2-hydroxyethyl)piperazin-1- yl]ethanesulfonic acid MOPS 3-(N-morpholino)propanesulfonic acid Pen/Strep penicillin and streptomycin HBS-P+ buffer containing 0.1M HEPES, 1.5M NaCl and 0.5% v/v Surfactant P20 DTT DL-Dithiothreitol BGG bovine gamma globulin PBMC peripheral blood mononuclear cells APC antigen presenting cells CD cluster of differentiation IgG immunoglobulin G OKT3 CD3 monoclonal antibody FLAG-Tag amino acid sequence DYKDDDDK DNA deoxyribonucleic acid CFSE carboxyfluorescein succinimidyl ester OVA ovalbumin antigen FACS fluorescence-activated cell sorting s.c. subcutaneous i.v. intravenous i.p. intraperitoneal n.d. not determined

Examples were tested in selected biological assays one or more times. When tested more than once, data are reported as either average values or as median values, wherein

-   -   the average value, also referred to as the arithmetic mean         value, represents the sum of the values obtained divided by the         number of times tested, and     -   the median value represents the middle number of the group of         values when ranked in ascending or descending order. If the         number of values in the data set is odd, the median is the         middle value. If the number of values in the data set is even,         the median is the arithmetic mean of the two middle values.

Examples were synthesized one or more times. When synthesized more than once, data from biological assays represent average values or median values calculated utilizing data sets obtained from testing of one or more synthetic batch.

The in vitro activity of the compounds of the present invention can be demonstrated in the following assays:

Human DGKζ Kinase Activity Inhibition Assay.

Human diacylglycerol kinase zeta (DGKζ) inhibitory activity of compounds of the present invention was quantified employing the human DGKζ kinase activity assay as described in the following paragraphs. In essence, the enzyme activity was measured by quantification of the adenosine-di-phosphate (ADP) generated as a co-product of the enzyme reaction via the “ADP-Glo™ Kinase Assay” kit from the company Promega. This detection system works as follows: In a first step the adenosine-tri-phosphate (ATP) not consumed in the kinase reaction is quantitatively converted to cyclic adenosine-mono-phosphate (cAMP) employing an adenylate cyclase (“ADP-Glo-reagent”), then the adenylate cyclase is stopped and the ADP generated in the kinase reaction is converted to ATP, which subsequently generates in a luciferase-based reaction a glow-luminescence signal (“Kinase Detection Reagent”).

C-terminally FLAG-tagged, recombinant full-length human DGKζ (inhouse expressed in baculovirus infected insect cells, purified using anti-Flag pulldown and size exclusion chromatography) was used as enzyme. As an alternative, commercially available enzyme by Carnabio can be used. As substrate for the kinase, 1,2-dioleoyl-sn-glycerol, reconstituted in octyl-β-D-glucopyranoside micelles, was used. For the preparation of the micelles, 1 volume of a 16.1 mM solution of 1,2-dioleoyl-sn-glycerol (Avanti, Cat. #08001-25G) in chloroform was slowly evaporated using a nitrogen stream. Subsequently, 22.55 volumes of a 510 mM solution of octyl-β-D-glucopyranoside (Sigma-Aldrich, Cat. #08001-10G) in 50 mM MOPS buffer (pH 7.4) were added, and the mixture was sonicated in an ultrasonic bath for 20 s. Then 35 volumes of 50 mM MOPS buffer (pH 7.4) were added to yield a solution of 0.28 mM 1,2 dioleoyl-sn-glycerol and 200 mM octyl-β-D-glucopyranoside, which was aliquoted, flash-frozen in liquid nitrogen, and stored at −20° C. until use. For each experiment, a fresh aliquot was quickly thawed and diluted 24-fold with aqueous assay buffer (described below) containing 95.7 μM adenosine triphosphate (Promega) to yield a 1.67-fold concentrated substrate solution.

For the assay 50 nl of a 100-fold concentrated solution of the test compound in dimethyl sulfoxide (DMSO, Sigma) was pipetted into either a white 1536-well or a white low-volume 384-well microtiter plate (both Greiner Bio-One, Frickenhausen, Germany). Subsequently, 2 μl of a solution of human DGK; in aqueous assay buffer [50 mM (3-(N-morpholino)propanesulfonic acid (MOPS, pH 7.4, Sigma-Aldrich), 1 mM dithiothreitol (DTT, Sigma-Aldrich), 100 mM NaCl (Sigma-Aldrich), 10 mM MgCl₂ (Sigma-Aldrich), 0.1% (w/v) bovine gamma globulin (BGG, Sigma-Aldrich), 1 μM CaCl₂ (Sigma-Aldrich)] were added to the wells, and the mixture was incubated for 15 min at 22° C. to allow pre-binding of the test compounds to the enzyme. The reaction was initiated by the addition of 3 μL of substrate solution [preparation described above; 11.7 μM 1,2-dioleoyl-sn-glycerol (=>final conc. in the 5 μL assay volume is 7 μM), 8.33 mM octyl-β-D-glucopyranoside (=>final conc. in 5 μL assay volume is 5 mM), and 91.67 μM adenosine triphosphate (=>final conc. in 5 μL assay volume is 55 μM) in assay buffer] and the resulting mixture was incubated for a reaction time of 20 min at 22° C. The concentration of DGKζ was adjusted depending of the activity of the enzyme lot and was chosen appropriate to have the assay in the linear range, a typical concentration is about 0.1 nM. The reaction was stopped by the addition of 2.5 μL of “ADP-Glo-reagent” (1 to 1.5 diluted with water) and the resulting mixture was incubated at 22° C. for 1 h to convert the ATP not consumed in the kinase reaction completely to cAMP. Subsequently 2.5 μl of the “kinase detection reagent” (1.2 fold more concentrated than recommended by the producer) were added, the resulting mixture was incubated at 22° C. for 1 h and then the luminescence measured with a suitable measurement instrument (e.g. Viewlux™ from Perkin-Elmer). The amount of emitted light was taken as a measure for the amount of ADP generated and thereby for the activity of the DGKζ.

The data were normalised (enzyme reaction without inhibitor=0% inhibition, all other assay components but no enzyme=100% inhibition). Usually the test compounds were tested on the same microtiterplate in 11 different concentrations in the range of 20 μM to 0.07 nM (20 μM, 5.7 μM, 1.6 μM, 0.47 μM, 0.13 μM, 38 nM, 11 nM, 3.1 nM, 0.9 nM, 0.25 nM and 0.07 nM, the dilution series prepared separately before the assay on the level of the 100 fold concentrated solutions in DMSO by serial dilutions, exact concentrations may vary depending pipettors used) in duplicate values for each concentration and IC₅₀ values were calculated using Genedata Screener™ software.

TABLE 18 IC₅₀ values of examples in in vitro human DGKζ kinase activity inhibition assays. Example number IC₅₀ [nM] 1 946 2 935 3 927 4 833 5 757 6 740 7 705 8 618 9 536 10 425 11 406 12 364 13 357 14 334 15 266 16 283 17 261 18 207 19 200 20 161 21 142 21.1 5510 21.2 51 22 130 23 130 24 115 25 101 26 74.8 27 69.6 27.1 8650 27.2 43.6 28 61 29 58.4 29.1 12500 29.2 46.2 30 57.5 31 43.5 32 38.7 33 42.8 33.1 8610 33.2 22.8 34 34.4 35 25.3 36 22.2 37 19.8 37.1 2000 37.2 9.46 38 19.3 38.1 2000 38.2 7.37 39 19 40 15.4 40.1 792 40.2 7.08 41 13.5 41.1 2930 41.2 7.73 42 11.3 43 11.1 43.1 2730 43.2 5.93 44 8.83 44.1 1390 44.2 6.72 45 338 45.1 201 45.2 8910 45.3 1050 45.4 >20000 46 7.07 46.1 1470 46.2 5.69 47 3.77 47.1 1020 47.2 1.96 48 3.72 48.1 1.76 48.2 120 49 2.76 49.1 1030 49.2 2.1 50 378 50.1 127 50.2 14300 51 16.6 51.1 8.85 51.2 872 52 99.2 52.1 72.7 52.2 11400 53 30.8 53.1 20.3 53.2 1780 54 20.7 55 9.74 55.1 6 55.2 835 56 120 56.1 50.5 56.2 7640 57 155 57.1 137 57.2 14638 58 16.7 58.1 8 58.2 1047 59 10.3 59.1 4 59.2 424 60 403 61 93.2 61.1 5180 61.2 108 62 32.2 62.1 4620 62.2 23.2 63 33.9 63.1 5170 63.2 18.5 64 55.8 65 101 66 363 67 2270 68 457 69 64.1 70 196 71 2310 72 985 73 148 73.1 3720 73.2 82.1 74 190 74.1 17100 74.2 81.4 75 419 75.1 >20000 75.2 214 76 252 76.1 114 76.2 11000 77 533 77.1 234 77.2 10200 78 7220 78.1 >20000 78.2 7550 79 6790 79.1 1660 79.2 2390 80 10.8 81 8.71 81.1 1020 81.2 10.1 82 89.6 83 24.3 84 24.6 85 20.3 86 759 87 8.53 88 667 89 131 90 63.3 91 701 92 16.7 93 187 94 25.7 95 39 96 31.4 97 24.7 98 73.5 99 75.8 100 176 101 329 102 506 103 41.9 104 234 105 6.45 106 33.3 107 77.3 108 19.4 109 51.7 110 366 111 334 112 300 113 703 114 658 115 272 116 581 117 14.9 118 22.4 119 4.54 120 16.7 121 19.9 122 9.07 123 100 124 48.3 125 44 126 28.8 127 73.1 128 72.2 129 97.1 130 46.5 131 180 132 93.3 133 42.5 134 76.8 135 49.5 136 740 137 238 138 61.6 139 56.9 140 37.5 141 389 142 14.5 143 12.7 144 8.79 145 2.66 146 21.6 147 291 148 76.4 149 405 150 372 151 387 152 111 153 107 154 55.6 155 200 156 231 157 190 158 6.71 159 214 160 187 160.1 115 160.2 8448 161 15 161.1 9 161.2 2573 162 22 162.1 12 162.2 6574 163 9 163.1 5 163.2 1091 164 7 164.1 5 164.2 944 165 3 165.1 2 165.2 331 166 3 166.1 2 166.2 129 167 2 167.1 3 167.2 303 168 19 169 3 169.1 3 169.2 859 170 7 170.1 5 170.2 1000 171 11 172 46 172.1 24 172.2 4892 173 25 174 51 174.1 26 174.2 3793 175 20 176 91 176.1 34 176.2 672 177 40 178 48 179 57 180 62 181 65 182 91 182.1 84 182.2 10527 183 222 184 428 184.1 297 184.2 >20000 185 301 186 392 187 1400 187.1 553 187.2 >20000 188 1240 189 6352 190 553 191 356 192 224 193 35 194 66 195 1000 196 128 197 90 198 88 199 396 200 70 201 25 202 69 203 381 204 73 205 54 206 48 207 22 208 672 209 45 210 69 211 183 212 41 213 42 214 16 215 279 216 32 217 41 218 64 219 13 220 219 221 31 222 108 223 235 224 45 225 252 226 87 227 393 228 218 229 193 230 199 231 662 232 112 233 267 234 1640 235 542 236 730 237 749 238 667 239 236 240 1328 241 382 242 2031 243 51 244 30 245 22 246 662 247 40 248 136 249 120 250 36 251 87 252 106 253 59 254 32 255 76 256 9 257 n.d. 258 19 259 19 260 29 261 90 262 6 263 539 264 376 265 459 266 287 267 63 268 3 269 12 270 13 271 18 272 39 273 20 274 107 275 29 276 29 277 494 278 230 279 48 280 43 281 98 282 53 283 36 284 33 285 68 285.1 34 285.2 432 286 31 287 18 288 3442 289 10 290 936 291 178 292 282

TABLE 19 IC₅₀ values of intermediates in in vitro human DGKζ kinase activity inhibition assays. Intermediate number IC₅₀ [nM] 41 >20000 43 >20000 62 >20000 63 >20000 64 >20000 65 >20000 66 >20000 67 >20000

Transactivation Assay in Jurkat IL2-Reporter Cell Line

Transactivation assays were carried out in Jurkat cells purchased from Promega (Promega, #CS187001) stably transfected with a firefly luciferase reporter gene construct under the control of the IL2-promoter. Cells were cultured as specified by the manufacturer. Bulk cells were harvested at a culture density of approx. 1 E+06 cells/mL, suspended in cryo-storage medium (70% RPMI/20% FCS/10% DMSO), frozen at controlled rate of −1°/min in 1.8 mL cryo-vials with cell densities of 1 E+07 to 1 E+08 cells per vial, and stored at −150° C. or below until further use. Frozen cells were thawed and cultured in medium at a starting density of 3.5 E+05 cells/mL for 6 days. On day 6 cells were centrifuged for 5 min at 300×g, medium was decanted and cell concentration was adjusted to 5.0 E+06 cells/mL with fresh assay medium (500 mL RPMI (Gibco, #22400)+5 mL L-Glutamin (Sigma, #G7513)+5 mL Penicillin/Streptomycin (Sigma #P0781)+5 mL Non-essential amino acids (Invitrogen, #11140)+5 mL sodium-pyruvate (Gibco #1136088), 5 mL FBS (Biochrom, #S0615)). Cell working stock was split in two parts: neutral control and compounds with EC30 stimulation, high control with EC100 stimulation.

An antibody premix was prepared by diluting anti-CD3 (BD Pharmingen, #555329), anti-CD28 (BD Pharmingen, #555725) and goat anti mouse anti-IgG (ThermoFisher, #31160) antibodies at 1/1/4 ratio in assay medium at 2-fold of final concentration (final concentrations depend on cell batch, typically for neutral control 0.055/0.055/0.22 μg/mL, for high control 0.5/0.5/2 mg/mL). The premix solutions were added to the cells in 1+1 volume prior use.

Fifty nL of a 100-fold concentrated solution of the test compounds in DMSO were transferred into a white microtiter test plate (384, Greiner Bio-One, Germany). For this, either a Hummingbird liquid handler (Digilab, USA) or an Echo acoustic system (Labcyte, USA) was used. Five μL of the freshly prepared cell suspension was added to the wells of a test plate and incubated at 37° C. in a 5% CO₂ atmosphere. After completion of the incubation for 4 hours, 3 μl of Bio-Glo Luciferase assay reagent (Promega, #G7941, prepared as recommended by the supplier) were added to all wells. The test plate was incubated at 20° C. for 10 min before measurement of the luminescence in a microplate reader (typically Pherastar by BMG, Germany, or ViewLux by Perkin-Elmer, USA). Data were normalized (neutral control=0% effect, high control=100% effect). Compounds were tested in duplicates at up to 11 concentrations (typically 20 μM, 5.7 μM, 1.6 μM, 0.47 μM, 0.13 μM, 38 nM, 11 nM, 3.1 nM, 0.89 nM, 0.25 nM and 0,073 nM). Dilution series were made prior to the assay in a 100-fold concentrated form by serial dilution. ECs, values were calculated by 4-Parameter fitting using a commercial software package (Genedata Analyzer, Switzerland).

Polyclonal Activation of Human PBMCs

To test the effect of DGKζ inhibitors of the present invention on IL-2 and IFN-γ secretion of human Peripheral Blood Mononuclear Cells (PBMCs) a 24 h human PBMC assay was performed as screening assay. For this, a 96 well flat bottom plate was coated with a suboptimal stimulation condition (EC 10-30) of human aCD3 (Invitrogen, clone OKT3) antibody in 50 μL PBS/well at 4° C. overnight. PBMCs isolated and frozen at liquid N₂ from leucapherese samples was thawed and resuspended in culture medium (X-Vivo-20). 4×10⁵ cells/well were plated. Wells were treated with the DGKζ inhibitors of the present invention at the respective concentrations (5-fold dilution steps from 10 μM to 3 nM) and the final DMSO concentration per well is 0.1%. Medium+DMSO (0.1%) was used as baseline value. As positive controls 1000 ng/mL aCD3+aCD28 (1 μg/mL) and a DGKζ reference inhibitor was used. After 24 h the medium was collected and hIL-2 or hIFN-γ ELISA were performed. The following parameters were calculated: EC₅₀ value, concentration at 50% increase; max increase in % and respective concentration and maximum effect normalized to max concentration (10 μM) of a selected DGKζ reference inhibitor.

In Vitro Activation of Mouse OT-I Antigen-Specific T-Cells

To test the effect of DGKζ inhibitors of the present invention in murine antigen-specific T-cells, spleens and lymph nodes of OT-I mice were collected and mashed through a 40 μm cell strainer and incubated for 1 min in 1 mL ACK lysing buffer (Gibco)/spleen. 4×106 cells/mL were incubated in medium containing 0.05 ng/mL SIINFEKL (FIG. 2 ) in a 50 mL falcon at 37° C. for 30 min. Afterwards cells were centrifuged and 4×106 cells/mL were resuspended in fresh medium (DMEM; 10% FCS, 1% Pen/Strep, 0.1% β-mercaptoethanol, 1% HEPES). 4×105 cells were plated per well in a 96-well round bottom plate. Wells were treated with DGKζ inhibitors of the present invention at the respective concentrations (5-fold dilution steps from 10 μM to 3 nM) in a final DMSO concentration of 0.1%. Medium+DMSO (0.1%) was used as baseline value. As positive controls cells incubated with the 4×SIINFEKL concentration (0.2 ng/ml) and a DGKζ reference inhibitor were used. The plates were centrifuged to reduce the distance between T-cells and APCs before incubation. After 24 h the medium was collected and mIL-2 or mIFN-γ ELISAs were performed. The following parameters were calculated: EC₅₀ value, concentration at 50% increase; max increase in % and respective concentration and maximum effect normalized to max concentration (10 μM) of a selected DGKζ reference inhibitor.

DGKζ Surface Plasmon Resonance Interaction Assay

The ability of the compounds described in this invention to bind to DGKζ were determined using surface plasmon resonance (SPR). This allows for the quantification of binding in terms of the equilibrium dissociation constant (K_(D) [M]), as well as association and dissociation rate constants (k_(on) [1/Ms] and k_(off) [1/s], respectively). The measurements were performed using Biacore® T200, Biacore® S200 or Biacore® 8K (GE Healthcare).

All buffers described in this section were prepared with 10×HBS-P+ Buffer (GE Healthcare, #BR100671) supplemented with additional buffer components as indicated below, dithiothreitol (DTT from Sigma, #D0632-25G), Adenosine 5′-triphosphate (ATP from Sigma, #A26209-10G), MgCl₂ (Sigma, #M1028-100 ML), dimethyl sulfoxide (DMSO from Biomol, #54686.500).

For SPR measurements, recombinant and biotinylated human DGKζ (obtained from Carna Biosciences, Product number: 12-410-20N) was immobilized via the streptavidin-biotin interaction onto a Series S Sensor Chip SA (GE Healthcare, #BR-1005-31). Briefly, DGKζ was diluted to a concentration of 10 μg/mL in Immobilization Buffer (10 mM HEPES, 150 mM NaCl, 0.05% v/v Surfactant P20, 2 mM MgCl₂, 1 mM DTT, pH 7.4) and captured on the SA Chip surface using a flow rate of 10 μL/min for 500 seconds at a temperature of 10° C. Immobilization levels of approximately 6000 RU were typically achieved. The reference surface consisted of a streptavidin surface without immobilized protein. Compounds were diluted from 10 mM DMSO stock solution into Running Buffer (10 mM HEPES, 150 mM NaCl, 0.05% v/v Surfactant P20, 2 mM MgCl₂, 1 mM DTT, 0.2 mM ATP and 1% v/v DMSO, pH 7.4). For SPR-binding measurements serial dilutions (typically 1:3 dilutions resulting in 8 concentrations up to 2 μM or 20 μM) were injected over immobilized protein. Binding affinity and kinetics were measured at 18° C. and at a flow rate of 100 μL/min.

A variation of the assay with an additional regeneration step was performed by injection of Regeneration Buffer without ATP (10 mM HEPES, 150 mM NaCl, 0.05% v/v Surfactant P20, 1 mM DTT and 1% v/v DMSO, pH 7.4) for 200 s at a flow rate of 30 μL/min

The double-referenced sensorgrams were fit to a simple reversible Langmuir 1:1 reaction mechanism as implemented in the Biacore® T200, S200 and 8K evaluation software (Biacore T200 Evaluation Software version 2.0, Biacore S200 Evaluation Software version 1.0, Biacore 8K Evaluation Software, GE Healthcare).

Expression of DGKζ in Insect Cells Using the Baculovirus System

Expression Constructs:

The cDNA encoding the full length sequence of human DGKζ (Uniprot Q13574-2) was optimized for expression in eukaryotic cells and synthesized by the GeneArt Technology at Life Technologies.

The DNA sequence encoded the following sequence:

Construct DGKζ_Hu Amino Acid M1 to V928

Additionally the expression construct encoded: a Kozak DNA sequence for translation initiation (GCCACC), a translational start codon for methionine followed by amino acid glycine, a Flag (DYKDDDDK) sequence at the N-terminus of DGKζ, and at the C-terminus of DGKζ two stop codons and moreover 5′ and 3′ att-DNA sequences for Gateway Cloning.

The DGKζ construct was subcloned using the Gateway Technology into the Destination vector pD-INS. The vector pD-INS is a Baculovirus transfer vector (based on vector pVL1393, Pharmingen) which enables the expression of the Flag-DGK) protein. The respective protein was named DGKz_hu_1.

Generation of Recombinant Baculovirus

The DGKζ transfer vector was co-transfected in Sf9 cells with Baculovirus DNA (Flashbac Gold DNA, Oxford Expression Technologies) using Fugene HD (Roche). After 5 days the supernatant of the transfected cells containing the recombinant Baculovirus encoding the various DGK proteins was used for further infection of Sf9 cells for virus amplification whereby the virus titer was monitored using qPCR.

DGKζ Expression in Sf9 Cells Using Bioreactor

Sf9 cells cultured (Insect-xpress medium, Lonza, 27° C.) in a Wave-bioreactor with a disposable culture bag were infected at a cell density of 10⁶ cells/mL with one of the recombinant baculovirus stocks at a multiplicity of infection of 1 and incubated for 72. Subsequently, the cells were harvested by centrifugation (800×g) and the cell pellet frozen at −80° C.

Purification of the DGKz_Hu_1 Protein:

Purification of the DGKz_hu_1 protein was achieved by a two-step chromatography procedure as follows.

The pelleted cells (from 8 L cell culture) were resuspended in Lysis-Buffer (25 mM Tris HCl 80; 500 mM NaCl; 250 mM Sucrose, 1 mM DTT; 0.1% Triton X-100; Complete Protease Inhibitor Cocktail-(Roche)) and lysed by a freeze-thaw cycle followed by an incubation on ice for 60 min in the presence of Benzonase (25 U/mL). The lysate was centrifuged at 63.000×g for 30 min at 4° C. The soluble supernatant was than incubated with 40 mL anti-Flag M2 Agarose (Sigma) in a plastic flask rotating for 16 h at 4° C. for binding of the tagged DGK; proteins, subsequently rinsed with 5×50 mL Wash-Buffer (25 mM Tris HCl 8.0; 500 mM NaCl; 250 mM Sucrose; 1 mM DTT) and finally the bound protein was eluted using Elution-Buffer (Wash-Buffer with 250 μg/mL FLAG-Peptide, incubated 30 min. at 4° C. with 3×25 mL).

The elution fractions from the affinity chromatography were concentrated (using Amicon Ultra 15, Centrifugal Filters, 30 kDa MW cut-off; Millipore #UFC903024) to 25 mL and applied to a size exclusion chromatography column (S200 prep grade 26/60, GE Healthcare) and the resulting monomeric peak fraction was collected, pooled and again concentrated. Wash-buffer with 300 mM NaCl was used for size exclusion chromatography and the final concentrated sample. The final protein sample concentration was 5 to 10 mg/mL and the yield was 5 mg final protein per L cell culture.

The in vivo activity of the compounds of the present invention can be demonstrated in the following assays:

In Vivo Activation of Murine Antigen Specific OT-I T Cells

Oral Administration of compounds enhances antigen-specific T cell activation in vivo.

Direct detection of antigen-specific T cell proliferation in vivo is technically challenging, since it requires the presence of T cells specific for a cognate antigen and also a specific measurement procedure for cell proliferation. Both these requirements are fulfilled in the OT-I transfer model, which utilizes the direct transfer of CD8 T cells transgenic for a T cell receptor recognizing an Ovalbumin-derived peptide as antigen.

Before transfer, the OT-I T cells were labeled with the fluorescent dye CFSE, which was diluted by every cell division and therefore allowed detection of cell proliferation. After transfer of the CFSE-labeled T cells, mice were vaccinated with the Ovalbumin antigen OVA-30 (FIG. 3 ). Only transferred OT-I cells were able to recognize the OVA-antigen presented by APC and only these transferred T cells then got activated. Flow cytometric analysis of CFSE-levels in the OT-I cells can be combined with measurement of multiple activation markers like CD69, CD25 and PD1. In particular, Wild type C57Bl6 mice received 2×10×6 CFSE-labeled OT-I T cells and were vaccinated one day later by intravenous application of 2.5 μg OVA-30. Mice were then divided into groups which received vehicle only, DGKζ inhibitors of the present invention alone or in combination with other immune modulating agents. Mice were treated for 2 to 20 days and T cell composition (incl. transferred OT-I cells) of spleen, blood and lymph nodes were analysed by FACS.

In Vivo Syngeneic Tumor Models

Mice were assigned to a study at the age of 6-8 weeks. Animal husbandry, feeding and health conditions were according to animal welfare guidelines. Syngeneic tumor cell lines were cultivated with appropriate medium and split at least 3 times before inoculation. Female mice were inoculated with appropriate amount of tumor cells in medium or a medium/matrigel mixture s.c, i.v., or i.p, depending on the model. After 4-10 days the mice were randomized and therapeutic treatment started when tumors had reached a size of approx. 40-70 mm².

Tumor size was measured using calipers determining length (a) and width (b). Tumor volume was calculated according to:

v=(a×b{circumflex over ( )}2)/2

Significance of monotherapies and combination treatment was calculated versus control group as determined by 2-Way ANOVA analysis. 

1-16. (canceled)
 17. A compound having the structure:

or a tautomer, hydrate, or solvate thereof, or a pharmaceutically acceptable salt of any of the foregoing.
 18. The compound of claim 17, wherein the compound is

or a tautomer, hydrate, or solvate thereof, or a pharmaceutically acceptable salt of any of the foregoing.
 19. The compound of claim 17, wherein the compound is

or a pharmaceutically acceptable salt thereof.
 20. The compound of claim 17, wherein the compound is


21. A pharmaceutical composition comprising a compound of claim 18, or a tautomer, hydrate, or solvate thereof, or a pharmaceutically acceptable salt of any of the foregoing, and at least one pharmaceutically acceptable excipient.
 22. A pharmaceutical composition comprising a compound of claim 19, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient.
 23. A pharmaceutical composition comprising a compound of claim 20 and at least one pharmaceutically acceptable excipient.
 24. A method of treating or preventing a disease or condition associated with dysregulated immune responses or aberrant DGKζ signaling in a mammal, comprising administering an effective amount of a compound of claim 18, or a tautomer, hydrate, or solvate thereof, or a pharmaceutically acceptable salt of any of the foregoing, to the mammal.
 25. The method of claim 24, wherein the mammal is a human.
 26. The method of claim 24, wherein the disease is cancer.
 27. The method of claim 24, wherein the compound or pharmaceutically acceptable salt thereof exercises its effect through T-cell activation.
 28. The method of claim 24, wherein the disease is non-small cell lung cancer, gastric adenocarcinoma, cancer of the gastroesophageal junction, clear cell renal cell carcinoma, or melanoma.
 29. The method of claim 24, wherein the disease is non-small cell lung cancer.
 30. The method of claim 24, wherein the disease is gastric adenocarcinoma.
 31. The method of claim 24, wherein the disease is cancer of the gastroesophageal junction.
 32. The method of claim 24, wherein the disease is clear cell renal cell carcinoma.
 33. The method of claim 24, wherein the disease is melanoma.
 34. A method of treating or preventing a disease or condition associated with dysregulated immune responses or aberrant DGKζ signaling in a mammal, comprising administering an effective amount of a compound of claim 19, or a pharmaceutically acceptable salt thereof, to the mammal.
 35. The method of claim 34, wherein the compound is (R)-2-(N-[4-amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4-fluoro-anilino)propanamide.
 36. The method of claim 34, wherein the mammal is a human.
 37. The method of claim 34, wherein the disease is cancer.
 38. The method of claim 34, wherein the compound or pharmaceutically acceptable salt thereof exercises its effect through T-cell activation.
 39. The method of claim 34, wherein the disease is non-small cell lung cancer, gastric adenocarcinoma, cancer of the gastroesophageal junction, clear cell renal cell carcinoma, or melanoma.
 40. The method of claim 34, wherein the disease is non-small cell lung cancer.
 41. The method of claim 34, wherein the disease is gastric adenocarcinoma.
 42. The method of claim 34, wherein the disease is cancer of the gastroesophageal junction.
 43. The method of claim 34, wherein the disease is clear cell renal cell carcinoma.
 44. The method of claim 34, wherein the disease is melanoma. 